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The map label for this gene is clpP

Identifier: 21672724

GI number: 21672724

Start: 521153

End: 521746

Strand: Direct

Name: clpP

Synonym: BUsg459

Alternate gene names: 21672724

Gene position: 521153-521746 (Clockwise)

Preceding gene: 21672723

Following gene: 21672725

Centisome position: 81.25

GC content: 29.12

Gene sequence:

>594_bases
ATGAAATCTAATTCAGTATTAATTCCAATGGTAGTCGAACAACATTCAAGAGGAGAACGTTCATATGACATATATTCTAG
ATTGTTGAAAGAACGAATAATTTTTATCACAGGTACAATTGAAGACAATATGGCAAGCTCAATTGTAGCACAAATGCTGT
TTTTAGAAGCAGAAAGTTTAGATAAAGATATATTTTTATATATAAATTCACCAGGAGGTATTATTACATCAGGGATGTCA
ATTTACGATACTATGCAATTTATAAAACCCGATGTTAACACTATTTGTATAGGACAAGCGTGTTCAATGGCAGCTTTTTT
ACTTTCATCTGGTACAAAGGGTAAAAGATCTTGTTTACCAAATGCAAGAATTATGATTCATCAACCATTAGGTGGATATC
AAGGACAAGCTTCAGATATTGCTATTCATGCAGAAGAGATTAACAAAATGAAAAAAAAATTAAACAAATTAATGTCTTTA
AATACGGGTCAATCTATTAAAAAAATTAATAAAGATACAGAACGAGATTGCTTTTTATCAGCACATGAGTCTATAAAATA
TGGATTAATTGATTTAATTTTAACTCAACGTTAA

Upstream 100 bases:

>100_bases
TATATTAGAAAAAAATTTTATAAAAATATCTTTTATGCCAAAAATTAAATTATCGATAATTTAAAAAAATATGTACTACT
ATATTCATAGAGAAAATTAA

Downstream 100 bases:

>100_bases
GAAAAATTTTTATTAAAAATAATAAAACAGAATCTATTTACTCAAAAAAAAATCGATAAATATATGAATTAAAGAGGTTA
AAAATATGACAGATAAGAGT

Product: ATP-dependent Clp protease proteolytic subunit

Products: NA

Alternate protein names: Endopeptidase Clp

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MKSNSVLIPMVVEQHSRGERSYDIYSRLLKERIIFITGTIEDNMASSIVAQMLFLEAESLDKDIFLYINSPGGIITSGMS
IYDTMQFIKPDVNTICIGQACSMAAFLLSSGTKGKRSCLPNARIMIHQPLGGYQGQASDIAIHAEEINKMKKKLNKLMSL
NTGQSIKKINKDTERDCFLSAHESIKYGLIDLILTQR

Sequences:

>Translated_197_residues
MKSNSVLIPMVVEQHSRGERSYDIYSRLLKERIIFITGTIEDNMASSIVAQMLFLEAESLDKDIFLYINSPGGIITSGMS
IYDTMQFIKPDVNTICIGQACSMAAFLLSSGTKGKRSCLPNARIMIHQPLGGYQGQASDIAIHAEEINKMKKKLNKLMSL
NTGQSIKKINKDTERDCFLSAHESIKYGLIDLILTQR
>Mature_197_residues
MKSNSVLIPMVVEQHSRGERSYDIYSRLLKERIIFITGTIEDNMASSIVAQMLFLEAESLDKDIFLYINSPGGIITSGMS
IYDTMQFIKPDVNTICIGQACSMAAFLLSSGTKGKRSCLPNARIMIHQPLGGYQGQASDIAIHAEEINKMKKKLNKLMSL
NTGQSIKKINKDTERDCFLSAHESIKYGLIDLILTQR

Specific function: Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins

COG id: COG0740

COG function: function code OU; Protease subunit of ATP-dependent Clp proteases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S14 family

Homologues:

Organism=Homo sapiens, GI5174419, Length=190, Percent_Identity=54.7368421052632, Blast_Score=226, Evalue=9e-60,
Organism=Escherichia coli, GI1786641, Length=192, Percent_Identity=72.9166666666667, Blast_Score=310, Evalue=3e-86,
Organism=Caenorhabditis elegans, GI17538017, Length=191, Percent_Identity=52.8795811518325, Blast_Score=218, Evalue=1e-57,
Organism=Drosophila melanogaster, GI20129427, Length=197, Percent_Identity=54.8223350253807, Blast_Score=233, Evalue=4e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CLPP_BUCAP (Q8K990)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660791.1
- ProteinModelPortal:   Q8K990
- SMR:   Q8K990
- MEROPS:   S14.001
- EnsemblBacteria:   EBBUCT00000000371
- GeneID:   1005706
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg459
- GeneTree:   EBGT00050000007912
- HOGENOM:   HBG558421
- OMA:   SPMEAQD
- ProtClustDB:   PRK00277
- BioCyc:   BAPH198804:BUSG459-MONOMER
- GO:   GO:0005737
- GO:   GO:0006508
- HAMAP:   MF_00444
- InterPro:   IPR001907
- InterPro:   IPR018215
- PANTHER:   PTHR10381
- PRINTS:   PR00127
- TIGRFAMs:   TIGR00493

Pfam domain/function: PF00574 CLP_protease

EC number: =3.4.21.92

Molecular weight: Translated: 21970; Mature: 21970

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS00382 CLP_PROTEASE_HIS; PS00381 CLP_PROTEASE_SER

Important sites: ACT_SITE 102-102 ACT_SITE 127-127

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSNSVLIPMVVEQHSRGERSYDIYSRLLKERIIFITGTIEDNMASSIVAQMLFLEAESL
CCCCCEEEEHEECCCCCCCCCHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHCC
DKDIFLYINSPGGIITSGMSIYDTMQFIKPDVNTICIGQACSMAAFLLSSGTKGKRSCLP
CCEEEEEECCCCCEEECCHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHCCCCCCHHCCC
NARIMIHQPLGGYQGQASDIAIHAEEINKMKKKLNKLMSLNTGQSIKKINKDTERDCFLS
CCEEEEECCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHH
AHESIKYGLIDLILTQR
HHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKSNSVLIPMVVEQHSRGERSYDIYSRLLKERIIFITGTIEDNMASSIVAQMLFLEAESL
CCCCCEEEEHEECCCCCCCCCHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHCC
DKDIFLYINSPGGIITSGMSIYDTMQFIKPDVNTICIGQACSMAAFLLSSGTKGKRSCLP
CCEEEEEECCCCCEEECCHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHCCCCCCHHCCC
NARIMIHQPLGGYQGQASDIAIHAEEINKMKKKLNKLMSLNTGQSIKKINKDTERDCFLS
CCEEEEECCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHH
AHESIKYGLIDLILTQR
HHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438