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The map label for this gene is tig

Identifier: 21672723

GI number: 21672723

Start: 519698

End: 521026

Strand: Direct

Name: tig

Synonym: BUsg458

Alternate gene names: 21672723

Gene position: 519698-521026 (Clockwise)

Preceding gene: 21672722

Following gene: 21672724

Centisome position: 81.02

GC content: 19.11

Gene sequence:

>1329_bases
ATGAAATTTTTTATCGAAAAAAACAAAAAAAATACTGATCGTGTCACAATTAACATTCCAAAAAAAATAGTGAGTAACGA
ACTTATTCGAGAATTTATCGAAATTAATAAAAAAACAAAAATAAATGGTTTTAGAAAAGGAAAAACTCCTATTAAAATTA
TACAAGAAAAATATGGTAATAGAGTTTATTATGATGTATTTAATCAACTAATGCAAAAATTTTTTTATGAATTTATAAAA
AAAGAAAAAATAAAAATTATTGGTTTACCAAAGTATGTTATGCATGAAAATGAAGACCAAAAAGAATATTTCAAATATTC
TGTAAACTACGAAGTTTATCCAAAATTTGAAATAAAAGATGTAAACCTTATTAAAGTAGAAAAAATAATAGTGAATATTA
AAGACGAAGATGTAAAAAAAAACATAGAAAAAACAGCATCTTATGAAAAAGATATATGGAATAAAGTTAATAGAGCAATT
AAAACTAACGATTTAGTGACAATTAATTACTGCATTTATGAAAATAATAAAAAACTAGACAAGTTTAACGTAGAAAAATT
TAAATTTATTGTTTCTCAAAATAATTTCATACCTGAATTAAATAACAAATTAATCAATCATTTTACCAATGATGTGATTT
TTTTTAAAATAAATTTTTGCAAATTTCATCCAGAAGAAGAATTACAAGGTAAAGATATAACATTTAAAATAAAAATATTA
AATGTTGAAGAAAAACAAGAAAATATTGAAATAGAAAAAAATATAAAAACAATTAAAATAAATAAACTAAGTAAATTAAA
CTATCAAACTATAAAAAATAATATTATTGAAAAAATAAAAAGCTTAACGCAAAATCATTTACAAAATCAAATCATAAAAC
AACTAATAATAAAAAATCCTATAAATATACCTCCAACTTTACTAAGAGAAGAAACAAATTTTTTACGCAATAAATTTATA
AAAGAATATAAAGAAAAGCAAGAAAACATTTTGAAAAAAAAATATCATACTAATCTTGAATCAAAAGCTAAAACGAGACT
ACATATAAAATTAATTATAGAAAAAATTATTCGTGATAACAAAATATCAGTAAATGAAGAAAAAGTAGACTTATTAATAA
AAAAAATATCTTTAAAGTATAAAAAACCATTGGAAATTATTAATATATATAAAAAGAATACAATGTTAAGAAAAACGATA
AAAAATATAGAATTAGAAATGCAAGTTATGCAATTTTTAATAAAAAAAGTTAAAATCATTGAAAAAAATTGGACTCTAGA
CGAAATTATGAATTATAACTGGAAAAATAACGAAGAACTTTTTGCTTAA

Upstream 100 bases:

>100_bases
TTTTACTTAAAAAATATGAAATATTAAAATATAGAATATTTTTTATAAATTAATAGAATATCTAAAAAAATTAAATAAAT
GTAATTATTTGAGGCAAAAA

Downstream 100 bases:

>100_bases
GATTAGTATTTTTTTTAATATTCTAATATATTAGAAAAAAATTTTATAAAAATATCTTTTATGCCAAAAATTAAATTATC
GATAATTTAAAAAAATATGT

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MKFFIEKNKKNTDRVTINIPKKIVSNELIREFIEINKKTKINGFRKGKTPIKIIQEKYGNRVYYDVFNQLMQKFFYEFIK
KEKIKIIGLPKYVMHENEDQKEYFKYSVNYEVYPKFEIKDVNLIKVEKIIVNIKDEDVKKNIEKTASYEKDIWNKVNRAI
KTNDLVTINYCIYENNKKLDKFNVEKFKFIVSQNNFIPELNNKLINHFTNDVIFFKINFCKFHPEEELQGKDITFKIKIL
NVEEKQENIEIEKNIKTIKINKLSKLNYQTIKNNIIEKIKSLTQNHLQNQIIKQLIIKNPINIPPTLLREETNFLRNKFI
KEYKEKQENILKKKYHTNLESKAKTRLHIKLIIEKIIRDNKISVNEEKVDLLIKKISLKYKKPLEIINIYKKNTMLRKTI
KNIELEMQVMQFLIKKVKIIEKNWTLDEIMNYNWKNNEELFA

Sequences:

>Translated_442_residues
MKFFIEKNKKNTDRVTINIPKKIVSNELIREFIEINKKTKINGFRKGKTPIKIIQEKYGNRVYYDVFNQLMQKFFYEFIK
KEKIKIIGLPKYVMHENEDQKEYFKYSVNYEVYPKFEIKDVNLIKVEKIIVNIKDEDVKKNIEKTASYEKDIWNKVNRAI
KTNDLVTINYCIYENNKKLDKFNVEKFKFIVSQNNFIPELNNKLINHFTNDVIFFKINFCKFHPEEELQGKDITFKIKIL
NVEEKQENIEIEKNIKTIKINKLSKLNYQTIKNNIIEKIKSLTQNHLQNQIIKQLIIKNPINIPPTLLREETNFLRNKFI
KEYKEKQENILKKKYHTNLESKAKTRLHIKLIIEKIIRDNKISVNEEKVDLLIKKISLKYKKPLEIINIYKKNTMLRKTI
KNIELEMQVMQFLIKKVKIIEKNWTLDEIMNYNWKNNEELFA
>Mature_442_residues
MKFFIEKNKKNTDRVTINIPKKIVSNELIREFIEINKKTKINGFRKGKTPIKIIQEKYGNRVYYDVFNQLMQKFFYEFIK
KEKIKIIGLPKYVMHENEDQKEYFKYSVNYEVYPKFEIKDVNLIKVEKIIVNIKDEDVKKNIEKTASYEKDIWNKVNRAI
KTNDLVTINYCIYENNKKLDKFNVEKFKFIVSQNNFIPELNNKLINHFTNDVIFFKINFCKFHPEEELQGKDITFKIKIL
NVEEKQENIEIEKNIKTIKINKLSKLNYQTIKNNIIEKIKSLTQNHLQNQIIKQLIIKNPINIPPTLLREETNFLRNKFI
KEYKEKQENILKKKYHTNLESKAKTRLHIKLIIEKIIRDNKISVNEEKVDLLIKKISLKYKKPLEIINIYKKNTMLRKTI
KNIELEMQVMQFLIKKVKIIEKNWTLDEIMNYNWKNNEELFA

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=437, Percent_Identity=30.4347826086957, Blast_Score=221, Evalue=9e-59,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_BUCAP (Q8K991)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660790.1
- ProteinModelPortal:   Q8K991
- SMR:   Q8K991
- EnsemblBacteria:   EBBUCT00000000341
- GeneID:   1005707
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg458
- GeneTree:   EBGT00050000007708
- HOGENOM:   HBG366224
- OMA:   ETAINDG
- ProtClustDB:   CLSK316279
- BioCyc:   BAPH198804:BUSG458-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 53503; Mature: 53503

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFFIEKNKKNTDRVTINIPKKIVSNELIREFIEINKKTKINGFRKGKTPIKIIQEKYGN
CCEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCC
RVYYDVFNQLMQKFFYEFIKKEKIKIIGLPKYVMHENEDQKEYFKYSVNYEVYPKFEIKD
EEHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCCCHHHEEEEECEEEECCEEECC
VNLIKVEKIIVNIKDEDVKKNIEKTASYEKDIWNKVNRAIKTNDLVTINYCIYENNKKLD
CCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEEEEEECCCCCC
KFNVEKFKFIVSQNNFIPELNNKLINHFTNDVIFFKINFCKFHPEEELQGKDITFKIKIL
CCCHHHEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEEEEEECCHHHCCCCCEEEEEEEE
NVEEKQENIEIEKNIKTIKINKLSKLNYQTIKNNIIEKIKSLTQNHLQNQIIKQLIIKNP
EECCHHHCCEEECCCEEEEEECHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
INIPPTLLREETNFLRNKFIKEYKEKQENILKKKYHTNLESKAKTRLHIKLIIEKIIRDN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
KISVNEEKVDLLIKKISLKYKKPLEIINIYKKNTMLRKTIKNIELEMQVMQFLIKKVKII
CCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKNWTLDEIMNYNWKNNEELFA
HCCCCHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKFFIEKNKKNTDRVTINIPKKIVSNELIREFIEINKKTKINGFRKGKTPIKIIQEKYGN
CCEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCC
RVYYDVFNQLMQKFFYEFIKKEKIKIIGLPKYVMHENEDQKEYFKYSVNYEVYPKFEIKD
EEHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCCCHHHEEEEECEEEECCEEECC
VNLIKVEKIIVNIKDEDVKKNIEKTASYEKDIWNKVNRAIKTNDLVTINYCIYENNKKLD
CCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEEEEEECCCCCC
KFNVEKFKFIVSQNNFIPELNNKLINHFTNDVIFFKINFCKFHPEEELQGKDITFKIKIL
CCCHHHEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEEEEEECCHHHCCCCCEEEEEEEE
NVEEKQENIEIEKNIKTIKINKLSKLNYQTIKNNIIEKIKSLTQNHLQNQIIKQLIIKNP
EECCHHHCCEEECCCEEEEEECHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
INIPPTLLREETNFLRNKFIKEYKEKQENILKKKYHTNLESKAKTRLHIKLIIEKIIRDN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
KISVNEEKVDLLIKKISLKYKKPLEIINIYKKNTMLRKTIKNIELEMQVMQFLIKKVKII
CCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKNWTLDEIMNYNWKNNEELFA
HCCCCHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438