Click here to switch to the map view.

The map label for this gene is ispA

Identifier: 21672714

GI number: 21672714

Start: 511562

End: 512446

Strand: Reverse

Name: ispA

Synonym: BUsg449

Alternate gene names: 21672714

Gene position: 512446-511562 (Counterclockwise)

Preceding gene: 30410823

Following gene: 21672713

Centisome position: 79.89

GC content: 24.75

Gene sequence:

>885_bases
ATGAATTTTTCTCATTTATATGACATCTACTATAATCGTATAAATAAAAAGTTGTTCGAAATAATACAAGAATTGCCCTT
TCAGGATTCTGTTCTTTTTAGAGCAATGAAATACAGTACCTTATCAGGAGGAAAAAGATTACGTGCATGTTTAATATATG
CTACTGGAGAAACATTTCAAGTCAATATTGCTGCACTTGATGTTATATCTGCTGCTGTTGAATTAGTTCATTCGTATTCT
TTAATTCATGATGATTTGCCATGTATTGATAACGATTATTTTAGAAGAGGGAAAATTTCTTGTCATATAAAGTATGGTGA
AAATTTTGCTTTATTGGCAGGTGATGCATTACAAGGATTGGCATTTAATATTTTATCAAATAGTAATATGCCAGGAGTAC
ACGATTCTATTCGTTTAAAAATGATTGCTGAGTTTTCTAATGCAATTGGTTATTCTGGGATGTGTATTGGTCAAATGCTA
GATTTAGAAAAAGAAAGAAAAAAAATAAATATATCTGAATTAGAAAAGATTAATTTATATAAAACTGCATTTTTAATTCG
GTGCTCTATACGTTTAGCTTATTTTGCTTCTAACAATTTTTCTAAAGAAGTTTTATTTATTTTAGATAAATTTTCAGTTT
CTATTGGTTTGGCTTTTCAAATTCAAGATGATATCTTGGATTTAAAAAATGATATCAAAAAGTTAGAGAGTAAAAGAAAT
AAAACAAAAAATACATATCCATTATTAATAGGATTAAAAAAATCTAAAATTAAAATAAAAGAATTATATAAAGAAGCTTT
TTTTACATTAGAAATTTTAAAAAAAAATTTTAATGTTAATATATTAAAATTACTGACACAGTTTATAATGAAACGTTTTA
AATAA

Upstream 100 bases:

>100_bases
ATAATTTTTTTATAGTATAATTTATATGAATATCTTAAATGTTTATATATTTTAATTTAATTTTTTATTAATTTTATAAT
TAATCTTTTAGGTGTTAAAT

Downstream 100 bases:

>100_bases
GTTTAAAAATGAGTTTCCAATGAATTTTGACATTACAAGATATCCAATTTTATCTTTTGCTCATTCAGTAAGACGTTTAC
GACTTTTATCGATTGAACAA

Product: geranyltranstransferase

Products: NA

Alternate protein names: FPP synthase; (2E,6E)-farnesyl diphosphate synthase; Geranyltranstransferase

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MNFSHLYDIYYNRINKKLFEIIQELPFQDSVLFRAMKYSTLSGGKRLRACLIYATGETFQVNIAALDVISAAVELVHSYS
LIHDDLPCIDNDYFRRGKISCHIKYGENFALLAGDALQGLAFNILSNSNMPGVHDSIRLKMIAEFSNAIGYSGMCIGQML
DLEKERKKINISELEKINLYKTAFLIRCSIRLAYFASNNFSKEVLFILDKFSVSIGLAFQIQDDILDLKNDIKKLESKRN
KTKNTYPLLIGLKKSKIKIKELYKEAFFTLEILKKNFNVNILKLLTQFIMKRFK

Sequences:

>Translated_294_residues
MNFSHLYDIYYNRINKKLFEIIQELPFQDSVLFRAMKYSTLSGGKRLRACLIYATGETFQVNIAALDVISAAVELVHSYS
LIHDDLPCIDNDYFRRGKISCHIKYGENFALLAGDALQGLAFNILSNSNMPGVHDSIRLKMIAEFSNAIGYSGMCIGQML
DLEKERKKINISELEKINLYKTAFLIRCSIRLAYFASNNFSKEVLFILDKFSVSIGLAFQIQDDILDLKNDIKKLESKRN
KTKNTYPLLIGLKKSKIKIKELYKEAFFTLEILKKNFNVNILKLLTQFIMKRFK
>Mature_294_residues
MNFSHLYDIYYNRINKKLFEIIQELPFQDSVLFRAMKYSTLSGGKRLRACLIYATGETFQVNIAALDVISAAVELVHSYS
LIHDDLPCIDNDYFRRGKISCHIKYGENFALLAGDALQGLAFNILSNSNMPGVHDSIRLKMIAEFSNAIGYSGMCIGQML
DLEKERKKINISELEKINLYKTAFLIRCSIRLAYFASNNFSKEVLFILDKFSVSIGLAFQIQDDILDLKNDIKKLESKRN
KTKNTYPLLIGLKKSKIKIKELYKEAFFTLEILKKNFNVNILKLLTQFIMKRFK

Specific function: Unknown

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family

Homologues:

Organism=Escherichia coli, GI1786623, Length=299, Percent_Identity=43.4782608695652, Blast_Score=252, Evalue=2e-68,
Organism=Escherichia coli, GI1789578, Length=239, Percent_Identity=24.6861924686192, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPA_BUCAP (Q8K9A0)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660781.1
- ProteinModelPortal:   Q8K9A0
- SMR:   Q8K9A0
- EnsemblBacteria:   EBBUCT00000000229
- GeneID:   1005716
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg449
- GeneTree:   EBGT00050000008059
- HOGENOM:   HBG732667
- OMA:   IRPFLVN
- ProtClustDB:   CLSK316270
- BioCyc:   BAPH198804:BUSG449-MONOMER
- GO:   GO:0005737
- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949
- Gene3D:   G3DSA:1.10.600.10
- PANTHER:   PTHR12001

Pfam domain/function: PF00348 polyprenyl_synt; SSF48576 Terpenoid_synth

EC number: =2.5.1.10

Molecular weight: Translated: 33881; Mature: 33881

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS00723 POLYPRENYL_SYNTHASE_1; PS00444 POLYPRENYL_SYNTHASE_2

Important sites: BINDING 45-45 BINDING 48-48 BINDING 77-77 BINDING 95-95 BINDING 96-96 BINDING 181-181 BINDING 182-182 BINDING 220-220

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFSHLYDIYYNRINKKLFEIIQELPFQDSVLFRAMKYSTLSGGKRLRACLIYATGETFQ
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEECCEEE
VNIAALDVISAAVELVHSYSLIHDDLPCIDNDYFRRGKISCHIKYGENFALLAGDALQGL
EEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCEEEEEEECCCEEEEECCHHHHH
AFNILSNSNMPGVHDSIRLKMIAEFSNAIGYSGMCIGQMLDLEKERKKINISELEKINLY
HHEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHH
KTAFLIRCSIRLAYFASNNFSKEVLFILDKFSVSIGLAFQIQDDILDLKNDIKKLESKRN
HHHEEEEEEEEEEEEECCCCCCHHHEEEEHHCEEEEEEEEEHHHHHHHHHHHHHHHHHHC
KTKNTYPLLIGLKKSKIKIKELYKEAFFTLEILKKNFNVNILKLLTQFIMKRFK
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNFSHLYDIYYNRINKKLFEIIQELPFQDSVLFRAMKYSTLSGGKRLRACLIYATGETFQ
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEECCEEE
VNIAALDVISAAVELVHSYSLIHDDLPCIDNDYFRRGKISCHIKYGENFALLAGDALQGL
EEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCEEEEEEECCCEEEEECCHHHHH
AFNILSNSNMPGVHDSIRLKMIAEFSNAIGYSGMCIGQMLDLEKERKKINISELEKINLY
HHEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHH
KTAFLIRCSIRLAYFASNNFSKEVLFILDKFSVSIGLAFQIQDDILDLKNDIKKLESKRN
HHHEEEEEEEEEEEEECCCCCCHHHEEEEHHCEEEEEEEEEHHHHHHHHHHHHHHHHHHC
KTKNTYPLLIGLKKSKIKIKELYKEAFFTLEILKKNFNVNILKLLTQFIMKRFK
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438