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The map label for this gene is mesJ

Identifier: 21672395

GI number: 21672395

Start: 116022

End: 117359

Strand: Reverse

Name: mesJ

Synonym: BUsg103

Alternate gene names: 21672395

Gene position: 117359-116022 (Counterclockwise)

Preceding gene: 21672405

Following gene: 21672382

Centisome position: 18.3

GC content: 22.57

Gene sequence:

>1338_bases
TTGATTGAAAAAATTATTAATCAATATCAAAAAAAATTATTTTTAATAGCATATAGTGGCGGAATCGATTCTACAGTTCT
TCTTTATAAAATGTTAAAAATAAAAGAAAAAAATCCTCAAATTAAAATACGTGCCATTCATATTAATCACAATCTTCATC
CATCTTCAAAAAAATGGGAAGAACACTGTATAAAAATTTGTCACAAATATAAAATACCAATAATTACTAAAGAAATTAAG
ATTCTTTTAAAAAAAAATATCGAAGAAACATTAAGAATTAAACGATATAATACTATTTATAATTATTTACTTAATGATGA
AATTTTACTTACTGGACATCATCTGAATGATCAATGTGAAACACTCTTTTTATCTTTAAAAAGAGGAAGTGGACCAACTG
GATTATCCGGAATGTCTATTGAAAATTTTTTAGGAAAAAAAAGAATAGTTCGTCCTTTTTTAACAAAAACTAAAAAAGAA
TTACAAAAATGGGCTTGCGAAAACAACTTAGAATCGATTGAAGATTTTAGTAATTTTAATATTGATTACGATCGTAATTT
TATACGTCATAAATTAATTCCTATATTAGAACAAAGATGGCCTTTTTTTTTAAAAAATTGTTTTCGAACAACAGTTATAT
GTCGCGAAGAAACTAAATTAAAAAATATATTTTTAAAAGAAAAAATTCAAAATCTTATTAATTTTGATGAATCTTTAAAT
ATTCAAAATTTTAAAAACATAAATAAAGAAGTATGTAAAGCACTTATTCGATATTGGATTTCTTTAAAAAATATAAAAAT
GCCATCATATAAAACAATTGAATGCATTTATAATGAAATAATATGCAGTAAGAAAGATTCTAATCCAAAAATAATTATAG
ACAAAAACGAGATCAGACGTTACAAAACATCTCTCTATTTTATAAAAATACAAAAAGATATCAGTAATATATTTTTGTTC
TGGCATAACACAGATAAAAAATTATTGCTTCCTGAAGATTTAGGATATTTAATAAAAAACGACAAAGGCTTTATTCTTCC
TTCACCTAAAAAAAATGAATTAATTAATATTCGTTTTCAACTTGAAGGTAAAATTCTCATTTTAGGACGAGAAAAAAGAA
GAAAAATCAAAAAAATATGGCAAGAACACAATATACCTCCTTGGTTGAGAAATAAAATTCCTCTTTTATTTTATAATAAT
CGTTTTATTAGCGCTATTGGTTTGTTTGTTATTAAGGAAAAAATTATTAACAAGGAAAAAGAAATTCAAAAAAATTGGAA
AATATCTTGGATCAACAACGTACATTTTAATAGAAAAAATTTCTTTTTATTTTATTAA

Upstream 100 bases:

>100_bases
GTTCGTAGCTCAATAGGTTAGAGCATTACCATGACATGGTAGAGGTTAGTGGTTCAAGTCCACTCGAACGTATTAAAAAA
TTTTACTAGGTTATCTGTAT

Downstream 100 bases:

>100_bases
AATCATCTAGTAAAATGATAAAAAAAATGAGATTTTATTTACATAAAAGAGATAATTGTAAAATTTTATCATGATTAATT
CTATTATATAATTTTTTAAA

Product: putative cell cycle protein MesJ

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MIEKIINQYQKKLFLIAYSGGIDSTVLLYKMLKIKEKNPQIKIRAIHINHNLHPSSKKWEEHCIKICHKYKIPIITKEIK
ILLKKNIEETLRIKRYNTIYNYLLNDEILLTGHHLNDQCETLFLSLKRGSGPTGLSGMSIENFLGKKRIVRPFLTKTKKE
LQKWACENNLESIEDFSNFNIDYDRNFIRHKLIPILEQRWPFFLKNCFRTTVICREETKLKNIFLKEKIQNLINFDESLN
IQNFKNINKEVCKALIRYWISLKNIKMPSYKTIECIYNEIICSKKDSNPKIIIDKNEIRRYKTSLYFIKIQKDISNIFLF
WHNTDKKLLLPEDLGYLIKNDKGFILPSPKKNELINIRFQLEGKILILGREKRRKIKKIWQEHNIPPWLRNKIPLLFYNN
RFISAIGLFVIKEKIINKEKEIQKNWKISWINNVHFNRKNFFLFY

Sequences:

>Translated_445_residues
MIEKIINQYQKKLFLIAYSGGIDSTVLLYKMLKIKEKNPQIKIRAIHINHNLHPSSKKWEEHCIKICHKYKIPIITKEIK
ILLKKNIEETLRIKRYNTIYNYLLNDEILLTGHHLNDQCETLFLSLKRGSGPTGLSGMSIENFLGKKRIVRPFLTKTKKE
LQKWACENNLESIEDFSNFNIDYDRNFIRHKLIPILEQRWPFFLKNCFRTTVICREETKLKNIFLKEKIQNLINFDESLN
IQNFKNINKEVCKALIRYWISLKNIKMPSYKTIECIYNEIICSKKDSNPKIIIDKNEIRRYKTSLYFIKIQKDISNIFLF
WHNTDKKLLLPEDLGYLIKNDKGFILPSPKKNELINIRFQLEGKILILGREKRRKIKKIWQEHNIPPWLRNKIPLLFYNN
RFISAIGLFVIKEKIINKEKEIQKNWKISWINNVHFNRKNFFLFY
>Mature_445_residues
MIEKIINQYQKKLFLIAYSGGIDSTVLLYKMLKIKEKNPQIKIRAIHINHNLHPSSKKWEEHCIKICHKYKIPIITKEIK
ILLKKNIEETLRIKRYNTIYNYLLNDEILLTGHHLNDQCETLFLSLKRGSGPTGLSGMSIENFLGKKRIVRPFLTKTKKE
LQKWACENNLESIEDFSNFNIDYDRNFIRHKLIPILEQRWPFFLKNCFRTTVICREETKLKNIFLKEKIQNLINFDESLN
IQNFKNINKEVCKALIRYWISLKNIKMPSYKTIECIYNEIICSKKDSNPKIIIDKNEIRRYKTSLYFIKIQKDISNIFLF
WHNTDKKLLLPEDLGYLIKNDKGFILPSPKKNELINIRFQLEGKILILGREKRRKIKKIWQEHNIPPWLRNKIPLLFYNN
RFISAIGLFVIKEKIINKEKEIQKNWKISWINNVHFNRKNFFLFY

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=418, Percent_Identity=36.6028708133971, Blast_Score=335, Evalue=3e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_BUCAP (Q8KA23)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660462.1
- ProteinModelPortal:   Q8KA23
- SMR:   Q8KA23
- EnsemblBacteria:   EBBUCT00000000429
- GeneID:   1005920
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg103
- GeneTree:   EBGT00050000007722
- HOGENOM:   HBG668702
- OMA:   KSAVGFF
- ProtClustDB:   CLSK552873
- BioCyc:   BAPH198804:BUSG103-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- SMART:   SM00977
- TIGRFAMs:   TIGR02433
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C; SSF56037 B3_4

EC number: NA

Molecular weight: Translated: 53550; Mature: 53550

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEKIINQYQKKLFLIAYSGGIDSTVLLYKMLKIKEKNPQIKIRAIHINHNLHPSSKKWE
CHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCHHHH
EHCIKICHKYKIPIITKEIKILLKKNIEETLRIKRYNTIYNYLLNDEILLTGHHLNDQCE
HHHHHHHHHCCCCEEHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHH
TLFLSLKRGSGPTGLSGMSIENFLGKKRIVRPFLTKTKKELQKWACENNLESIEDFSNFN
HHEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
IDYDRNFIRHKLIPILEQRWPFFLKNCFRTTVICREETKLKNIFLKEKIQNLINFDESLN
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHCCCCCCC
IQNFKNINKEVCKALIRYWISLKNIKMPSYKTIECIYNEIICSKKDSNPKIIIDKNEIRR
CHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEHHHHHH
YKTSLYFIKIQKDISNIFLFWHNTDKKLLLPEDLGYLIKNDKGFILPSPKKNELINIRFQ
EEEEEEEEEEEECCCEEEEEEECCCCEEECCCCCCCEEECCCCEECCCCCCCCEEEEEEE
LEGKILILGREKRRKIKKIWQEHNIPPWLRNKIPLLFYNNRFISAIGLFVIKEKIINKEK
ECCEEEEEECHHHHHHHHHHHHCCCCCHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCHH
EIQKNWKISWINNVHFNRKNFFLFY
HHHHCCEEEEEECEEECCCEEEEEC
>Mature Secondary Structure
MIEKIINQYQKKLFLIAYSGGIDSTVLLYKMLKIKEKNPQIKIRAIHINHNLHPSSKKWE
CHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCHHHH
EHCIKICHKYKIPIITKEIKILLKKNIEETLRIKRYNTIYNYLLNDEILLTGHHLNDQCE
HHHHHHHHHCCCCEEHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHH
TLFLSLKRGSGPTGLSGMSIENFLGKKRIVRPFLTKTKKELQKWACENNLESIEDFSNFN
HHEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
IDYDRNFIRHKLIPILEQRWPFFLKNCFRTTVICREETKLKNIFLKEKIQNLINFDESLN
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHCCCCCCC
IQNFKNINKEVCKALIRYWISLKNIKMPSYKTIECIYNEIICSKKDSNPKIIIDKNEIRR
CHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEHHHHHH
YKTSLYFIKIQKDISNIFLFWHNTDKKLLLPEDLGYLIKNDKGFILPSPKKNELINIRFQ
EEEEEEEEEEEECCCEEEEEEECCCCEEECCCCCCCEEECCCCEECCCCCCCCEEEEEEE
LEGKILILGREKRRKIKKIWQEHNIPPWLRNKIPLLFYNNRFISAIGLFVIKEKIINKEK
ECCEEEEEECHHHHHHHHHHHHCCCCCHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCHH
EIQKNWKISWINNVHFNRKNFFLFY
HHHHCCEEEEEECEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438