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The map label for this gene is dapA

Identifier: 21672380

GI number: 21672380

Start: 100663

End: 101547

Strand: Reverse

Name: dapA

Synonym: BUsg088

Alternate gene names: 21672380

Gene position: 101547-100663 (Counterclockwise)

Preceding gene: 21672382

Following gene: 21672375

Centisome position: 15.83

GC content: 30.4

Gene sequence:

>885_bases
ATGTTCAAAGGAAGTATTGTTGCATTAATTACACCAATGAATGAAGAAGGTGAAATTTGTCATTCAAGCTTAAAAAAGCT
AATTGATTATCATGTATTAAATAAAACAAAAGCTATTGTTTCTATTGGAACGACTGGAGAATCGGCTACTCTTAGTCAAG
AAGAACATATTGAAGTAGTTATGCTAACTTTAAAACTTGCAAATAAACGCATTCCTATCATTGCAGGTACAGGTGCGAAT
GCGACAACAGAAGCAATATCCTTAACAAAAAGATTTGAAAAATCAGGAATTGAAGCATGTCTTACTGTTACACCATACTA
TAACAAACCAACTCAAGAAGGATTATACCAGCATTTTAAAGCAATTTCAGAAAACACAAAATTACCACAAATTTTATATA
ATGTTCCAAGTCGAACTGGATGTGATTTACTTCCATCAACTGTGGCAAGATTATCTGAATTTAAAAATATTATTGGAATT
AAAGAAGCGACAGGTGATCTATCAAGAATACATAAAATTAAAAAATTGGTTAAAGACGATTTTTTATTAATTAGTGGTGA
TGACCCTACGGCTTTAGATTTTATTCAATTAGGTGGTCAAGGAGTGATATCAGTTACAGCAAATATTGCTGCAAAAGAAA
TGACACAGATGTGTTCATATGCACTTGAAGGTAACTTTAAAAGTGCAAGATCTATAAATGAACGTTTGATGTTATTACAT
GAATCTTTATTTATAGAACCTAATCCTATTCCAATAAAATGGTTAGCTAAAAAAATAGGTTTAATAAAAAGTGATACACT
TCGACTACCGATGACACCAATTTTAAATTCTACACGTACACAAATTGAAAAAGCACTTCAATATGCTAATCTTCAAAAGA
TATAA

Upstream 100 bases:

>100_bases
CAATTTTTTATAACTATGAGATTTATAACTTTTATTTACTTAATTTTAGATTTGTATGAAAGATACCATAAAGTATTTTT
TATGGAGGAGAACAAAATAA

Downstream 100 bases:

>100_bases
ATTTTTTTTTAAAGTAATGATCACAATTTTTAAAATTGGAATTTTTTCTTTAATAACATCTTGCTCCTTAAAAAATAATG
ACATTCTTTTTAAGAATGAG

Product: dihydrodipicolinate synthase

Products: NA

Alternate protein names: DHDPS

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MFKGSIVALITPMNEEGEICHSSLKKLIDYHVLNKTKAIVSIGTTGESATLSQEEHIEVVMLTLKLANKRIPIIAGTGAN
ATTEAISLTKRFEKSGIEACLTVTPYYNKPTQEGLYQHFKAISENTKLPQILYNVPSRTGCDLLPSTVARLSEFKNIIGI
KEATGDLSRIHKIKKLVKDDFLLISGDDPTALDFIQLGGQGVISVTANIAAKEMTQMCSYALEGNFKSARSINERLMLLH
ESLFIEPNPIPIKWLAKKIGLIKSDTLRLPMTPILNSTRTQIEKALQYANLQKI

Sequences:

>Translated_294_residues
MFKGSIVALITPMNEEGEICHSSLKKLIDYHVLNKTKAIVSIGTTGESATLSQEEHIEVVMLTLKLANKRIPIIAGTGAN
ATTEAISLTKRFEKSGIEACLTVTPYYNKPTQEGLYQHFKAISENTKLPQILYNVPSRTGCDLLPSTVARLSEFKNIIGI
KEATGDLSRIHKIKKLVKDDFLLISGDDPTALDFIQLGGQGVISVTANIAAKEMTQMCSYALEGNFKSARSINERLMLLH
ESLFIEPNPIPIKWLAKKIGLIKSDTLRLPMTPILNSTRTQIEKALQYANLQKI
>Mature_294_residues
MFKGSIVALITPMNEEGEICHSSLKKLIDYHVLNKTKAIVSIGTTGESATLSQEEHIEVVMLTLKLANKRIPIIAGTGAN
ATTEAISLTKRFEKSGIEACLTVTPYYNKPTQEGLYQHFKAISENTKLPQILYNVPSRTGCDLLPSTVARLSEFKNIIGI
KEATGDLSRIHKIKKLVKDDFLLISGDDPTALDFIQLGGQGVISVTANIAAKEMTQMCSYALEGNFKSARSINERLMLLH
ESLFIEPNPIPIKWLAKKIGLIKSDTLRLPMTPILNSTRTQIEKALQYANLQKI

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; first step. [C]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family

Homologues:

Organism=Homo sapiens, GI31543060, Length=243, Percent_Identity=27.5720164609054, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI13540533, Length=249, Percent_Identity=28.1124497991968, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1788823, Length=291, Percent_Identity=69.7594501718213, Blast_Score=449, Evalue=1e-128,
Organism=Escherichia coli, GI87082415, Length=288, Percent_Identity=27.7777777777778, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1786463, Length=241, Percent_Identity=26.5560165975104, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1789620, Length=233, Percent_Identity=25.3218884120172, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): DAPA_BUCAP (Q8KA24)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660447.1
- ProteinModelPortal:   Q8KA24
- SMR:   Q8KA24
- EnsemblBacteria:   EBBUCT00000000493
- GeneID:   1005905
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg088
- GeneTree:   EBGT00050000008107
- HOGENOM:   HBG358848
- OMA:   AQGNFVE
- ProtClustDB:   PRK03170
- BioCyc:   BAPH198804:BUSG088-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00418
- InterPro:   IPR013785
- InterPro:   IPR005263
- InterPro:   IPR002220
- InterPro:   IPR020625
- InterPro:   IPR020624
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR12128
- PRINTS:   PR00146
- TIGRFAMs:   TIGR00674

Pfam domain/function: PF00701 DHDPS

EC number: =4.2.1.52

Molecular weight: Translated: 32452; Mature: 32452

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: PS00665 DHDPS_1; PS00666 DHDPS_2

Important sites: ACT_SITE 161-161 BINDING 106-106

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKGSIVALITPMNEEGEICHSSLKKLIDYHVLNKTKAIVSIGTTGESATLSQEEHIEVV
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHE
MLTLKLANKRIPIIAGTGANATTEAISLTKRFEKSGIEACLTVTPYYNKPTQEGLYQHFK
EEEEHHCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHH
AISENTKLPQILYNVPSRTGCDLLPSTVARLSEFKNIIGIKEATGDLSRIHKIKKLVKDD
HHHCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHCC
FLLISGDDPTALDFIQLGGQGVISVTANIAAKEMTQMCSYALEGNFKSARSINERLMLLH
EEEEECCCCCHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ESLFIEPNPIPIKWLAKKIGLIKSDTLRLPMTPILNSTRTQIEKALQYANLQKI
HHHEECCCCCCHHHHHHHHCCCCCCCEECCCHHHHCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFKGSIVALITPMNEEGEICHSSLKKLIDYHVLNKTKAIVSIGTTGESATLSQEEHIEVV
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHE
MLTLKLANKRIPIIAGTGANATTEAISLTKRFEKSGIEACLTVTPYYNKPTQEGLYQHFK
EEEEHHCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHH
AISENTKLPQILYNVPSRTGCDLLPSTVARLSEFKNIIGIKEATGDLSRIHKIKKLVKDD
HHHCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHCC
FLLISGDDPTALDFIQLGGQGVISVTANIAAKEMTQMCSYALEGNFKSARSINERLMLLH
EEEEECCCCCHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ESLFIEPNPIPIKWLAKKIGLIKSDTLRLPMTPILNSTRTQIEKALQYANLQKI
HHHEECCCCCCHHHHHHHHCCCCCCCEECCCHHHHCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438