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The map label for this gene is dnaT

Identifier: 21672317

GI number: 21672317

Start: 22755

End: 23255

Strand: Reverse

Name: dnaT

Synonym: BUsg023

Alternate gene names: 21672317

Gene position: 23255-22755 (Counterclockwise)

Preceding gene: 21672320

Following gene: 21672316

Centisome position: 3.63

GC content: 24.95

Gene sequence:

>501_bases
ATGAAAATTTTAGTTTCAAAAAAAATAACTCTTGACTTATTTTGCAAAAATCCAATTCAAATTATAGAAAAAGAAAAAAA
TGAAATTATATCTATATCAAAAAACAAAAAAACTGTATTTTATGTAATACAGCCAAAAGCTTTAAAAAAATTATTTGATA
TTAAAGAATATTTTGTAAAAAGTAAAATAACAAAAAAAGAAAAAAAAATCATAGAAAAGTTTCCAATGCATACTAATTGG
ATTCCCGACAAAGATTTTATACAAAAAGCTGCATTATGGGGAATATCATTAAACGAAGAAGTTTCAAAATATGAACTTGC
TTCCTTTATTTCCTATTGGGAAGCAGAAGGTTTATTTTTTCATCATATACAATGGCAACAGAAATTAGCTAGAAGTTTAG
AACGAAGTCGATCTATGAATAATGCAATACAACAAAAAAAAGATATTACTTATATACCAGTACCTGATCAAAAAACACCT
GATGGATTTCGAGGCAAGTAA

Upstream 100 bases:

>100_bases
ATAAGAAGAAAACTACAAATAGAATAGCATGTTATCTTTACTTTTTTAAAATTTAAAAAATAAAAGTCATTGTAATAAAA
GCAAATAGGATCAAATATTC

Downstream 100 bases:

>100_bases
TGACATTTTATAATGAATTTTTTAAACGCCTTCAACGTCTTATGCCTAGTAATGTAAAACCAAAATTTGAAAGTGATCAA
GATTTATTAGCTTGGAATCA

Product: primosomal protein DnaI

Products: NA

Alternate protein names: Primosomal protein I

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MKILVSKKITLDLFCKNPIQIIEKEKNEIISISKNKKTVFYVIQPKALKKLFDIKEYFVKSKITKKEKKIIEKFPMHTNW
IPDKDFIQKAALWGISLNEEVSKYELASFISYWEAEGLFFHHIQWQQKLARSLERSRSMNNAIQQKKDITYIPVPDQKTP
DGFRGK

Sequences:

>Translated_166_residues
MKILVSKKITLDLFCKNPIQIIEKEKNEIISISKNKKTVFYVIQPKALKKLFDIKEYFVKSKITKKEKKIIEKFPMHTNW
IPDKDFIQKAALWGISLNEEVSKYELASFISYWEAEGLFFHHIQWQQKLARSLERSRSMNNAIQQKKDITYIPVPDQKTP
DGFRGK
>Mature_166_residues
MKILVSKKITLDLFCKNPIQIIEKEKNEIISISKNKKTVFYVIQPKALKKLFDIKEYFVKSKITKKEKKIIEKFPMHTNW
IPDKDFIQKAALWGISLNEEVSKYELASFISYWEAEGLFFHHIQWQQKLARSLERSRSMNNAIQQKKDITYIPVPDQKTP
DGFRGK

Specific function: This protein is required for primosome-dependent normal DNA replication; it is also involved in inducing stable DNA replication during SOS response. It forms, in concert with dnaB protein and other prepriming proteins dnaC, N, N', N'' a prepriming protein

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaT family

Homologues:

Organism=Escherichia coli, GI1790824, Length=177, Percent_Identity=36.1581920903955, Blast_Score=136, Evalue=7e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNAT_BUCAP (Q8KA78)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660384.1
- EnsemblBacteria:   EBBUCT00000000043
- GeneID:   1005839
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg023
- GeneTree:   EBGT00050000008058
- HOGENOM:   HBG416722
- ProtClustDB:   PRK02854
- BioCyc:   BAPH198804:BUSG023-MONOMER
- HAMAP:   MF_01061
- InterPro:   IPR020917

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 19665; Mature: 19665

Theoretical pI: Translated: 10.38; Mature: 10.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVSKKITLDLFCKNPIQIIEKEKNEIISISKNKKTVFYVIQPKALKKLFDIKEYFVK
CEEEECCCCEEEEEECCHHHHHHHCCCCEEEEECCCEEEEEEECCHHHHHHHHHHHHHHH
SKITKKEKKIIEKFPMHTNWIPDKDFIQKAALWGISLNEEVSKYELASFISYWEAEGLFF
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEE
HHIQWQQKLARSLERSRSMNNAIQQKKDITYIPVPDQKTPDGFRGK
EHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MKILVSKKITLDLFCKNPIQIIEKEKNEIISISKNKKTVFYVIQPKALKKLFDIKEYFVK
CEEEECCCCEEEEEECCHHHHHHHCCCCEEEEECCCEEEEEEECCHHHHHHHHHHHHHHH
SKITKKEKKIIEKFPMHTNWIPDKDFIQKAALWGISLNEEVSKYELASFISYWEAEGLFF
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEE
HHIQWQQKLARSLERSRSMNNAIQQKKDITYIPVPDQKTPDGFRGK
EHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438