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The map label for this gene is nfrA [H]

Identifier: 213693293

GI number: 213693293

Start: 2748444

End: 2749208

Strand: Direct

Name: nfrA [H]

Synonym: Blon_2447

Alternate gene names: 213693293

Gene position: 2748444-2749208 (Clockwise)

Preceding gene: 213693292

Following gene: 213693294

Centisome position: 97.02

GC content: 60.65

Gene sequence:

>765_bases
ATGGTTACCAACGCAACAATCGAGGCTCTTCTGGGCCGCCGCTCCATCCGTAAGTTCAAGGACGAGGCCATCGACGACGA
TACCCGCGCCACGCTCGAAACCGTCGCCCAGCATGCCGCGTCCAGCCAGTTCCTTAACGACTGGTCCGCCATCCGCATCA
CCGATCCGGCCATCAAAGCCAAGCTCGCCGAATTCGGCCATCAGCCGTATATTGCCACTGCGCCGCTGCTGTACGTGTTC
GTCATCGACGAGCACCGCAACGCGCGCATCGCCGAACGCAAGGGCATCGATCCCGCTTCCGACGAATTCCATCTCAAGTA
CAGTTATCGCTTTACCCAAGCTCAGAACGACGCAGTGCTGGCCCTGCACGCCATGGAGACCGCCGCCTATTCGCTGGGAC
TGGGCTGCGTGATTCTCGGCTCTCTACTCAACAACATCCCCGGCCTTATCGACGTGCTGAATCTGCCGGAATACACGTAT
CCGGTGCTGGGTCTGGCCATCGGCAAGCCTGATCAGAACCCGACCGTCAAGCCGCGTATGCCGCGCACGATGCAGTTCTT
CGAGAACACCTACCCGGCTGACGCCGATGGAGTACTCGACCAGCTGCCCGCCTTCGACGAAGAAGTTCACCGGTACTACG
ATCTGCGCCAAGCCGACCGTCCGGTGGACGCCTTCAGCGATCAGGTCGTCACAATCAGTCGGCAGGATGTCAGCCATCAG
ACGCTGCTCGACAAGGCCGCCGCTCAGGGCTTCCGTCTGGATTAG

Upstream 100 bases:

>100_bases
AGGAACCATCAGTCCAGCACAGTTCCGCCACACAATATCGGCATTTCACTGTGAGGTAATTCACCGTGGCGTCCGGCGTC
TGCCGTGCGACAATAAAGCC

Downstream 100 bases:

>100_bases
TGAGACCAGGACCAACGAGATAGGCTCGGGATGGGCTCGTCACTGAGTCGTCTCAGTCTCAGCACTGCGACGACTCAGTC
CCAACACCTCAACATTTCAT

Product: nitroreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MVTNATIEALLGRRSIRKFKDEAIDDDTRATLETVAQHAASSQFLNDWSAIRITDPAIKAKLAEFGHQPYIATAPLLYVF
VIDEHRNARIAERKGIDPASDEFHLKYSYRFTQAQNDAVLALHAMETAAYSLGLGCVILGSLLNNIPGLIDVLNLPEYTY
PVLGLAIGKPDQNPTVKPRMPRTMQFFENTYPADADGVLDQLPAFDEEVHRYYDLRQADRPVDAFSDQVVTISRQDVSHQ
TLLDKAAAQGFRLD

Sequences:

>Translated_254_residues
MVTNATIEALLGRRSIRKFKDEAIDDDTRATLETVAQHAASSQFLNDWSAIRITDPAIKAKLAEFGHQPYIATAPLLYVF
VIDEHRNARIAERKGIDPASDEFHLKYSYRFTQAQNDAVLALHAMETAAYSLGLGCVILGSLLNNIPGLIDVLNLPEYTY
PVLGLAIGKPDQNPTVKPRMPRTMQFFENTYPADADGVLDQLPAFDEEVHRYYDLRQADRPVDAFSDQVVTISRQDVSHQ
TLLDKAAAQGFRLD
>Mature_254_residues
MVTNATIEALLGRRSIRKFKDEAIDDDTRATLETVAQHAASSQFLNDWSAIRITDPAIKAKLAEFGHQPYIATAPLLYVF
VIDEHRNARIAERKGIDPASDEFHLKYSYRFTQAQNDAVLALHAMETAAYSLGLGCVILGSLLNNIPGLIDVLNLPEYTY
PVLGLAIGKPDQNPTVKPRMPRTMQFFENTYPADADGVLDQLPAFDEEVHRYYDLRQADRPVDAFSDQVVTISRQDVSHQ
TLLDKAAAQGFRLD

Specific function: Reduces FMN, organic nitro compounds and disulfide DTNB. Involved in maintenance of the cellular redox state and the disulfide stress response [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=248, Percent_Identity=35.4838709677419, Blast_Score=117, Evalue=8e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016446
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 28376; Mature: 28376

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTNATIEALLGRRSIRKFKDEAIDDDTRATLETVAQHAASSQFLNDWSAIRITDPAIKA
CCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHH
KLAEFGHQPYIATAPLLYVFVIDEHRNARIAERKGIDPASDEFHLKYSYRFTQAQNDAVL
HHHHHCCCCCEECCCEEEEEEEECCCCCCHHHHCCCCCCCCCEEEEEEEEEECCCCCEEE
ALHAMETAAYSLGLGCVILGSLLNNIPGLIDVLNLPEYTYPVLGLAIGKPDQNPTVKPRM
EEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHEEEECCCCCCCCCCCCC
PRTMQFFENTYPADADGVLDQLPAFDEEVHRYYDLRQADRPVDAFSDQVVTISRQDVSHQ
CHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCHHHHCCCEEEEECCCCHHH
TLLDKAAAQGFRLD
HHHHHHHHCCCCCC
>Mature Secondary Structure
MVTNATIEALLGRRSIRKFKDEAIDDDTRATLETVAQHAASSQFLNDWSAIRITDPAIKA
CCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHH
KLAEFGHQPYIATAPLLYVFVIDEHRNARIAERKGIDPASDEFHLKYSYRFTQAQNDAVL
HHHHHCCCCCEECCCEEEEEEEECCCCCCHHHHCCCCCCCCCEEEEEEEEEECCCCCEEE
ALHAMETAAYSLGLGCVILGSLLNNIPGLIDVLNLPEYTYPVLGLAIGKPDQNPTVKPRM
EEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHEEEECCCCCCCCCCCCC
PRTMQFFENTYPADADGVLDQLPAFDEEVHRYYDLRQADRPVDAFSDQVVTISRQDVSHQ
CHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCHHHHCCCEEEEECCCCHHH
TLLDKAAAQGFRLD
HHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA