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The map label for this gene is leuB [H]

Identifier: 213692926

GI number: 213692926

Start: 2285180

End: 2286211

Strand: Reverse

Name: leuB [H]

Synonym: Blon_2070

Alternate gene names: 213692926

Gene position: 2286211-2285180 (Counterclockwise)

Preceding gene: 213692927

Following gene: 213692925

Centisome position: 80.71

GC content: 62.21

Gene sequence:

>1032_bases
ATGGCAAAGACATATAAAATCGCCGTTATTCCCGGCGACGGTATTGGCAAGGAAGTCACTCCGTGGGCTCAGAAGGCTTT
GGAAAAGGCCGCTGAAGGCGTAGCTGACTTCGAGTATGAGAACTTCGACTTGGGTGCCGAGCGCTATCTGCGCGATGGTG
CGATCCTGCCCGAGGACGAAGAGGGGCGCATCAAGGCCAACGACGCGATTCTGCTCGGTGCCGTGGGCGATCCGCGTATC
AAGGCCGGCATTCTGGAACGCGGCCTGCTGCTGAAGCTGCGCTTCGACCTCGACCAGTACGTGAACCTGCGCCCCTCCAA
GCTGTACAAGGGCGTCACCTCGCCGCTCGCCAACCCCGGCGACATCGACTTCGTGGTGGTGCGTGAAGGCACCGAAGGTC
TGTACTGCGGTGCCGGCGGCGCGGTGCGCCGCAACACCCCGCAGGAGGTGGCCACCGAGGTGTCCATCAACACCGCATAC
GGTGTGGAGCGTGTGGTGCGTTACGCCTTCAAGCTTGCTATGAAGCGCAAGAAGCACGTCACCCTGGTGCACAAGAAGAA
CGTGCTCGTCAACGCCGGTGATATGTGGCAGCGCATCGTCGACAAGGTCGGCGAGGAATACCCTGAGGTCACCCACGACT
ACCAGCACATCGATGCGGCCACCATCTTCCTGGTCTCCGACCCGAGCCGTTTCGATGTGATTCTCACCGACAACCTCTTC
GGCGACATTCTGACCGATGAGGCCGGCTCCGTGGTGGGCGGCGTGGGCTACTCCGCCTCCGGCTGCATCAACGCCTCCGA
CGAGTTCCCGTCCATGTTCGAGCCGATTCACGGTTCCGCTCCGGACATCGCCGGTCAGAACAAGGCCAACCCGACCGCCG
CCATCCTCTCCGCCGCCATGCTGCTCGAGCATCTCGGCTTTGATGATGCGGCCAAGAAGATTCACACCGCCGTCGAAGCG
GACATCGAGGAGCTCGGCTCCACCGTGCGCTCCACCGACCAGGTCGGCAAGGACATTCTGGCCCGCATGTGA

Upstream 100 bases:

>100_bases
TGACCTAATGAATGTCTCGACACGATACGCATGTTGGTTGTGTCCACTAGCTGAGCGGGTTCGGGCCGGCAGGGGGTCGC
GCGAGTAGGCTCCTTGAACT

Downstream 100 bases:

>100_bases
CCTCAAACTTGGCTCCCCTTCAGAGGGGAGCCAGAAAACTCTCTATACTGAGTTGCATGCAAATACGACATGGGGAATGC
AAAACCGTTGGCGGCAAACT

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MAKTYKIAVIPGDGIGKEVTPWAQKALEKAAEGVADFEYENFDLGAERYLRDGAILPEDEEGRIKANDAILLGAVGDPRI
KAGILERGLLLKLRFDLDQYVNLRPSKLYKGVTSPLANPGDIDFVVVREGTEGLYCGAGGAVRRNTPQEVATEVSINTAY
GVERVVRYAFKLAMKRKKHVTLVHKKNVLVNAGDMWQRIVDKVGEEYPEVTHDYQHIDAATIFLVSDPSRFDVILTDNLF
GDILTDEAGSVVGGVGYSASGCINASDEFPSMFEPIHGSAPDIAGQNKANPTAAILSAAMLLEHLGFDDAAKKIHTAVEA
DIEELGSTVRSTDQVGKDILARM

Sequences:

>Translated_343_residues
MAKTYKIAVIPGDGIGKEVTPWAQKALEKAAEGVADFEYENFDLGAERYLRDGAILPEDEEGRIKANDAILLGAVGDPRI
KAGILERGLLLKLRFDLDQYVNLRPSKLYKGVTSPLANPGDIDFVVVREGTEGLYCGAGGAVRRNTPQEVATEVSINTAY
GVERVVRYAFKLAMKRKKHVTLVHKKNVLVNAGDMWQRIVDKVGEEYPEVTHDYQHIDAATIFLVSDPSRFDVILTDNLF
GDILTDEAGSVVGGVGYSASGCINASDEFPSMFEPIHGSAPDIAGQNKANPTAAILSAAMLLEHLGFDDAAKKIHTAVEA
DIEELGSTVRSTDQVGKDILARM
>Mature_342_residues
AKTYKIAVIPGDGIGKEVTPWAQKALEKAAEGVADFEYENFDLGAERYLRDGAILPEDEEGRIKANDAILLGAVGDPRIK
AGILERGLLLKLRFDLDQYVNLRPSKLYKGVTSPLANPGDIDFVVVREGTEGLYCGAGGAVRRNTPQEVATEVSINTAYG
VERVVRYAFKLAMKRKKHVTLVHKKNVLVNAGDMWQRIVDKVGEEYPEVTHDYQHIDAATIFLVSDPSRFDVILTDNLFG
DILTDEAGSVVGGVGYSASGCINASDEFPSMFEPIHGSAPDIAGQNKANPTAAILSAAMLLEHLGFDDAAKKIHTAVEAD
IEELGSTVRSTDQVGKDILARM

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=335, Percent_Identity=32.5373134328358, Blast_Score=171, Evalue=6e-43,
Organism=Homo sapiens, GI4758582, Length=321, Percent_Identity=30.5295950155763, Blast_Score=128, Evalue=8e-30,
Organism=Homo sapiens, GI28178838, Length=321, Percent_Identity=30.5295950155763, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI28178816, Length=319, Percent_Identity=27.2727272727273, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI28178821, Length=319, Percent_Identity=27.2727272727273, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI28178819, Length=160, Percent_Identity=31.25, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1788101, Length=333, Percent_Identity=45.945945945946, Blast_Score=268, Evalue=4e-73,
Organism=Escherichia coli, GI87081683, Length=361, Percent_Identity=39.3351800554017, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1787381, Length=366, Percent_Identity=30.6010928961749, Blast_Score=125, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI71986051, Length=347, Percent_Identity=32.5648414985591, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17550882, Length=338, Percent_Identity=31.9526627218935, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI25144293, Length=346, Percent_Identity=30.0578034682081, Blast_Score=118, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17505779, Length=316, Percent_Identity=28.7974683544304, Blast_Score=105, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6322097, Length=361, Percent_Identity=34.0720221606648, Blast_Score=166, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6319830, Length=360, Percent_Identity=34.7222222222222, Blast_Score=158, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6324709, Length=336, Percent_Identity=33.0357142857143, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6324291, Length=331, Percent_Identity=32.6283987915408, Blast_Score=142, Evalue=9e-35,
Organism=Drosophila melanogaster, GI24643270, Length=322, Percent_Identity=33.2298136645963, Blast_Score=175, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24643268, Length=322, Percent_Identity=33.2298136645963, Blast_Score=175, Evalue=5e-44,
Organism=Drosophila melanogaster, GI24661184, Length=339, Percent_Identity=31.858407079646, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI161078635, Length=341, Percent_Identity=29.9120234604106, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI161078633, Length=341, Percent_Identity=29.9120234604106, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24650122, Length=341, Percent_Identity=29.9120234604106, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI161078637, Length=341, Percent_Identity=29.9120234604106, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI161078639, Length=337, Percent_Identity=30.2670623145401, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI281362242, Length=340, Percent_Identity=29.7058823529412, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24648872, Length=340, Percent_Identity=29.7058823529412, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI20130355, Length=341, Percent_Identity=24.0469208211144, Blast_Score=99, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001804 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 37175; Mature: 37043

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTYKIAVIPGDGIGKEVTPWAQKALEKAAEGVADFEYENFDLGAERYLRDGAILPEDE
CCCEEEEEEEECCCCCCCCCHHHHHHHHHHHCCHHCCCCCCCCCCHHHHHHCCCCCCCCC
EGRIKANDAILLGAVGDPRIKAGILERGLLLKLRFDLDQYVNLRPSKLYKGVTSPLANPG
CCCEEECCEEEEECCCCCHHHHHHHCCCEEEEEEECHHHHCCCCHHHHHHHHHCCCCCCC
DIDFVVVREGTEGLYCGAGGAVRRNTPQEVATEVSINTAYGVERVVRYAFKLAMKRKKHV
CEEEEEEECCCCEEEECCCCCCCCCCHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHCE
TLVHKKNVLVNAGDMWQRIVDKVGEEYPEVTHDYQHIDAATIFLVSDPSRFDVILTDNLF
EEEEECCEEEECHHHHHHHHHHHHHHCHHHHCCHHHCCEEEEEEEECCCCEEEEEECCCH
GDILTDEAGSVVGGVGYSASGCINASDEFPSMFEPIHGSAPDIAGQNKANPTAAILSAAM
HHHHHCCCCCEEECCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
LLEHLGFDDAAKKIHTAVEADIEELGSTVRSTDQVGKDILARM
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKTYKIAVIPGDGIGKEVTPWAQKALEKAAEGVADFEYENFDLGAERYLRDGAILPEDE
CCEEEEEEEECCCCCCCCCHHHHHHHHHHHCCHHCCCCCCCCCCHHHHHHCCCCCCCCC
EGRIKANDAILLGAVGDPRIKAGILERGLLLKLRFDLDQYVNLRPSKLYKGVTSPLANPG
CCCEEECCEEEEECCCCCHHHHHHHCCCEEEEEEECHHHHCCCCHHHHHHHHHCCCCCCC
DIDFVVVREGTEGLYCGAGGAVRRNTPQEVATEVSINTAYGVERVVRYAFKLAMKRKKHV
CEEEEEEECCCCEEEECCCCCCCCCCHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHCE
TLVHKKNVLVNAGDMWQRIVDKVGEEYPEVTHDYQHIDAATIFLVSDPSRFDVILTDNLF
EEEEECCEEEECHHHHHHHHHHHHHHCHHHHCCHHHCCEEEEEEEECCCCEEEEEECCCH
GDILTDEAGSVVGGVGYSASGCINASDEFPSMFEPIHGSAPDIAGQNKANPTAAILSAAM
HHHHHCCCCCEEECCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
LLEHLGFDDAAKKIHTAVEADIEELGSTVRSTDQVGKDILARM
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA