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The map label for this gene is vfr [H]

Identifier: 213692924

GI number: 213692924

Start: 2283244

End: 2283963

Strand: Reverse

Name: vfr [H]

Synonym: Blon_2068

Alternate gene names: 213692924

Gene position: 2283963-2283244 (Counterclockwise)

Preceding gene: 213692925

Following gene: 213692923

Centisome position: 80.63

GC content: 59.03

Gene sequence:

>720_bases
ATGAGCTTGGAAACGCCAAACGCTTTTGCTGACGAGGAGAACATCCTGCTGCATACGGCATTGTTCAAGCAGGTATCGCC
AGCCGAAGCCGAGGAGCTGTTGCCGCATTTGAAGCGTGCGGCGTTCGATAAGGGCGACTATATCTTCTCCGAAGGCGATA
CCGATCAGCGCATGTATTTACTGGAGCAGGGGCGCGTCAAGCTGATTCGCACTTCTTCCGATGACCGCGTGCAACTGCTG
TCCATCCACGCGCCGGGCGAAGTGCTTGGCGAGATTCCGGTATTCGACCCCCATGGTGGCCCGCGCACCGCGTCAGCAGT
GGCCATGAGCCATGGAACGCGCGTGGTCTGGCTGGAGCATGATGCCCTGTTCGCATGGCTTGATGAGCATCCGCGTGTGG
CCATCGACATGTTGCAGGTCATGGCCAACCGCCAGCGCGGCAACAACGAACGCATCTCCGACTTGGTATTCATGGATGTG
CCGGCGCGTCTGGCCAAGATATTGCTCAACTTGGGCTCACGTTTCGGCGAGCCGGTGGAACAGGGTCTCAAAGTGCCGCA
TGACCTGACTCAGGAGGAGCTGGCCCAACTGGTGGGCTCCAGTCGTGAGACCGTGAACAAGGCGTTGATGGACTTTTCCA
ACCGCGGATGGATCGCACGTGACGGCCGATCGATCATCATCTTCCAGCCAGGTATGCTGATTCGACGCTCGCGGCGCTGA

Upstream 100 bases:

>100_bases
ACCTCATCGACTGGTTCTCCGCATGAGCGTCCGCGCCGAACGGGCGCGTTACAATATGACAGTCGAGGTCAGGCACGGGA
CGATCGATAAGGATGGTGCG

Downstream 100 bases:

>100_bases
CCGTTCGGCTGACTTCCAGCCGATGTTACGCTAGTGCGGTTCTTGTGATGATATGTTCACGGGAGCTGACCGTAAGCCCG
GTTGGTAGGGCACCCCATTT

Product: transcriptional regulator, Crp/Fnr family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MSLETPNAFADEENILLHTALFKQVSPAEAEELLPHLKRAAFDKGDYIFSEGDTDQRMYLLEQGRVKLIRTSSDDRVQLL
SIHAPGEVLGEIPVFDPHGGPRTASAVAMSHGTRVVWLEHDALFAWLDEHPRVAIDMLQVMANRQRGNNERISDLVFMDV
PARLAKILLNLGSRFGEPVEQGLKVPHDLTQEELAQLVGSSRETVNKALMDFSNRGWIARDGRSIIIFQPGMLIRRSRR

Sequences:

>Translated_239_residues
MSLETPNAFADEENILLHTALFKQVSPAEAEELLPHLKRAAFDKGDYIFSEGDTDQRMYLLEQGRVKLIRTSSDDRVQLL
SIHAPGEVLGEIPVFDPHGGPRTASAVAMSHGTRVVWLEHDALFAWLDEHPRVAIDMLQVMANRQRGNNERISDLVFMDV
PARLAKILLNLGSRFGEPVEQGLKVPHDLTQEELAQLVGSSRETVNKALMDFSNRGWIARDGRSIIIFQPGMLIRRSRR
>Mature_238_residues
SLETPNAFADEENILLHTALFKQVSPAEAEELLPHLKRAAFDKGDYIFSEGDTDQRMYLLEQGRVKLIRTSSDDRVQLLS
IHAPGEVLGEIPVFDPHGGPRTASAVAMSHGTRVVWLEHDALFAWLDEHPRVAIDMLQVMANRQRGNNERISDLVFMDVP
ARLAKILLNLGSRFGEPVEQGLKVPHDLTQEELAQLVGSSRETVNKALMDFSNRGWIARDGRSIIIFQPGMLIRRSRR

Specific function: Can bind cyclic AMP. Is a global regulator of virulence factor expression and is required for exotoxin A and protease production [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=201, Percent_Identity=23.3830845771144, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR018488
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 26899; Mature: 26768

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLETPNAFADEENILLHTALFKQVSPAEAEELLPHLKRAAFDKGDYIFSEGDTDQRMYL
CCCCCCCCCCCCCCEEEHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCEEEE
LEQGRVKLIRTSSDDRVQLLSIHAPGEVLGEIPVFDPHGGPRTASAVAMSHGTRVVWLEH
EECCCEEEEEECCCCCEEEEEEECCHHHHCCCCCCCCCCCCCHHHHHHHCCCCEEEEEEC
DALFAWLDEHPRVAIDMLQVMANRQRGNNERISDLVFMDVPARLAKILLNLGSRFGEPVE
CCEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHEEEECCHHHHHHHHHHHHHHCCHHHH
QGLKVPHDLTQEELAQLVGSSRETVNKALMDFSNRGWIARDGRSIIIFQPGMLIRRSRR
HHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCEEECCCEEEEECCCHHHHCCCC
>Mature Secondary Structure 
SLETPNAFADEENILLHTALFKQVSPAEAEELLPHLKRAAFDKGDYIFSEGDTDQRMYL
CCCCCCCCCCCCCEEEHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCEEEE
LEQGRVKLIRTSSDDRVQLLSIHAPGEVLGEIPVFDPHGGPRTASAVAMSHGTRVVWLEH
EECCCEEEEEECCCCCEEEEEEECCHHHHCCCCCCCCCCCCCHHHHHHHCCCCEEEEEEC
DALFAWLDEHPRVAIDMLQVMANRQRGNNERISDLVFMDVPARLAKILLNLGSRFGEPVE
CCEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHEEEECCHHHHHHHHHHHHHHCCHHHH
QGLKVPHDLTQEELAQLVGSSRETVNKALMDFSNRGWIARDGRSIIIFQPGMLIRRSRR
HHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCEEECCCEEEEECCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8002577; 10984043 [H]