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The map label for this gene is rihB [H]

Identifier: 213692866

GI number: 213692866

Start: 2191207

End: 2192163

Strand: Reverse

Name: rihB [H]

Synonym: Blon_2007

Alternate gene names: 213692866

Gene position: 2192163-2191207 (Counterclockwise)

Preceding gene: 213692867

Following gene: 213692865

Centisome position: 77.39

GC content: 65.31

Gene sequence:

>957_bases
ATGACAAAGCTCATTCTCGACCTTGACACCGGTGTGGATGACGCGCTCGCCATCGCATACGCATTGGGCAGTCCGGAAGT
GGAATTGATCGGCATCACCGGCACATACGGCAATGTGCTGCTCGACCAGGGCGTGCGCAACGCGCTGGCCATCACCGATC
TGCTCGGCCACCCGGAAGTCGGGGTGTACAAGGGTCTGCCGCACTCCAGCACCACCGACTCGTTCGAGGTGCTGGAGATC
TCCAAGTTCATCCACGGCGCCAACGGCATAGGCGACGTCTCGATCCCCGATTCGAACCGCGAACCGCAATCCGAGCCGGC
GGTCGACTTCATCATCGACGCGGCCAAGACGTACGGCAAGGAGCTCGTCTACGTGCCCACCGGCCCGATGACCAACATCG
CCGCCGCATTGCGCAAGGCGCCGGAGATCAAGGACGAGATCGGCCGCATCGTGCTTATGGGCGGTGCGCTGACCGTCTGC
GGCAACGTGAACGCCTGGGAGGAGGCGAACATCTCCCAGGATCCCGACGCCGCCGATTACCTGTTCCGTTCGGGCGCCCC
GGCCACGATGATCGGCCTCGACGTGACGCTCCAGACCCTGCTCACCTACCGGGAGACCCGGCAGTGGCGTGATCTGGGCA
CCAAGGCCGGCGCGTTCCTGGCGGACATGACCGACTTCTACATCAAGGCATACGAGACGACCTCCCCGCATCTGGGCGGC
TGCGGCCTGCACGACCCCCTGGCGGTCGGCGTGGCCGTGGACCCCACGCTGGTCGACACCCTGCCCATGAACCTCAAGGT
GGATGTGGACGGCCCCACGCGCGGCCGCACCATCGGCGACAATACGCGCCTCAACGATCCGGTCAAGACCATGAAGGCCG
CGGTCGGCGTCGACGTGCCGCGTTTCCTGCACGAGTTCATGACCCGCATCACCGCGCTCGCCAAGCACGCCGGGTAA

Upstream 100 bases:

>100_bases
TCTGACGCGCACCATCGCGGCATGGCGGCTATCATAGATGATGCTTGCGGGGGAATCAGTGACGGCCATTCGGTCTCGCC
CCCCAAGGGAAGGGTGTTTC

Downstream 100 bases:

>100_bases
CGGCGTCGGCGCACGGAACATGCCGAACACCGCAAACAGAGCATAGCAAACAGAGAACAGAGAGGAATTCCATGACATCA
TCGACCAACACCGCGGACGT

Product: Inosine/uridine-preferring nucleoside hydrolase

Products: NA

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MTKLILDLDTGVDDALAIAYALGSPEVELIGITGTYGNVLLDQGVRNALAITDLLGHPEVGVYKGLPHSSTTDSFEVLEI
SKFIHGANGIGDVSIPDSNREPQSEPAVDFIIDAAKTYGKELVYVPTGPMTNIAAALRKAPEIKDEIGRIVLMGGALTVC
GNVNAWEEANISQDPDAADYLFRSGAPATMIGLDVTLQTLLTYRETRQWRDLGTKAGAFLADMTDFYIKAYETTSPHLGG
CGLHDPLAVGVAVDPTLVDTLPMNLKVDVDGPTRGRTIGDNTRLNDPVKTMKAAVGVDVPRFLHEFMTRITALAKHAG

Sequences:

>Translated_318_residues
MTKLILDLDTGVDDALAIAYALGSPEVELIGITGTYGNVLLDQGVRNALAITDLLGHPEVGVYKGLPHSSTTDSFEVLEI
SKFIHGANGIGDVSIPDSNREPQSEPAVDFIIDAAKTYGKELVYVPTGPMTNIAAALRKAPEIKDEIGRIVLMGGALTVC
GNVNAWEEANISQDPDAADYLFRSGAPATMIGLDVTLQTLLTYRETRQWRDLGTKAGAFLADMTDFYIKAYETTSPHLGG
CGLHDPLAVGVAVDPTLVDTLPMNLKVDVDGPTRGRTIGDNTRLNDPVKTMKAAVGVDVPRFLHEFMTRITALAKHAG
>Mature_317_residues
TKLILDLDTGVDDALAIAYALGSPEVELIGITGTYGNVLLDQGVRNALAITDLLGHPEVGVYKGLPHSSTTDSFEVLEIS
KFIHGANGIGDVSIPDSNREPQSEPAVDFIIDAAKTYGKELVYVPTGPMTNIAAALRKAPEIKDEIGRIVLMGGALTVCG
NVNAWEEANISQDPDAADYLFRSGAPATMIGLDVTLQTLLTYRETRQWRDLGTKAGAFLADMTDFYIKAYETTSPHLGGC
GLHDPLAVGVAVDPTLVDTLPMNLKVDVDGPTRGRTIGDNTRLNDPVKTMKAAVGVDVPRFLHEFMTRITALAKHAG

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively. Has a clear preference for cytidine over uridine. Strictly specific for ribonucleosides [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788486, Length=302, Percent_Identity=36.0927152317881, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1786871, Length=301, Percent_Identity=33.2225913621262, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1786213, Length=314, Percent_Identity=32.1656050955414, Blast_Score=105, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI32565474, Length=292, Percent_Identity=30.1369863013699, Blast_Score=97, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17565698, Length=302, Percent_Identity=27.4834437086093, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6320608, Length=256, Percent_Identity=27.734375, Blast_Score=89, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24583915, Length=202, Percent_Identity=32.1782178217822, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24641839, Length=215, Percent_Identity=29.3023255813954, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24641837, Length=222, Percent_Identity=28.3783783783784, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022977 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: =3.2.2.8 [H]

Molecular weight: Translated: 33945; Mature: 33814

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLILDLDTGVDDALAIAYALGSPEVELIGITGTYGNVLLDQGVRNALAITDLLGHPEV
CCEEEEEECCCCCHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCC
GVYKGLPHSSTTDSFEVLEISKFIHGANGIGDVSIPDSNREPQSEPAVDFIIDAAKTYGK
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
ELVYVPTGPMTNIAAALRKAPEIKDEIGRIVLMGGALTVCGNVNAWEEANISQDPDAADY
EEEEECCCCHHHHHHHHHHCCCHHHHHCEEEEECCEEEEECCCCCCCCCCCCCCCCHHHH
LFRSGAPATMIGLDVTLQTLLTYRETRQWRDLGTKAGAFLADMTDFYIKAYETTSPHLGG
HHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEECCCCCCCCC
CGLHDPLAVGVAVDPTLVDTLPMNLKVDVDGPTRGRTIGDNTRLNDPVKTMKAAVGVDVP
CCCCCCEEEEEEECCHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHH
RFLHEFMTRITALAKHAG
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKLILDLDTGVDDALAIAYALGSPEVELIGITGTYGNVLLDQGVRNALAITDLLGHPEV
CEEEEEECCCCCHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCC
GVYKGLPHSSTTDSFEVLEISKFIHGANGIGDVSIPDSNREPQSEPAVDFIIDAAKTYGK
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
ELVYVPTGPMTNIAAALRKAPEIKDEIGRIVLMGGALTVCGNVNAWEEANISQDPDAADY
EEEEECCCCHHHHHHHHHHCCCHHHHHCEEEEECCEEEEECCCCCCCCCCCCCCCCHHHH
LFRSGAPATMIGLDVTLQTLLTYRETRQWRDLGTKAGAFLADMTDFYIKAYETTSPHLGG
HHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEECCCCCCCCC
CGLHDPLAVGVAVDPTLVDTLPMNLKVDVDGPTRGRTIGDNTRLNDPVKTMKAAVGVDVP
CCCCCCEEEEEEECCHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHH
RFLHEFMTRITALAKHAG
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA