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The map label for this gene is tuf [H]

Identifier: 213692784

GI number: 213692784

Start: 2097982

End: 2099181

Strand: Reverse

Name: tuf [H]

Synonym: Blon_1922

Alternate gene names: 213692784

Gene position: 2099181-2097982 (Counterclockwise)

Preceding gene: 213692785

Following gene: 213692783

Centisome position: 74.1

GC content: 63.5

Gene sequence:

>1200_bases
ATGGCAAAGGAAAAGTACGAGCGTACTAAGCCGCACGTTAACATCGGTACCATCGGCCACGTCGACCACGGTAAGACTAC
CCTGACTGCCGCCATCTCCAAGGTGCTGCACGAGGAGTTCCCGGATGTCAACCCGGAGTACGACTTCAACCAGATCGATT
CCGCTCCGGAAGAGGCCGCCCGCGGTATCACCATCAACATCGCCCACATCGAGTACCAGACCGAGAAGCGTCACTACGCT
CACGTCGACTGCCCGGGCCACGCCGACTTCGTGAAGAACATGATCACCGGTGCCGCCCAGATGGATGGCGCCATCCTCGT
TGTGGCCGCCACCGACGGCCCGATGGCCCAGACCCGCGAGCACGTGCTGCTCGCCCGTCAGGTCGGCGTTCCGAAGATCC
TCGTCGCCCTCAACAAGTGCGACATGGTCGACGATGAAGAGCTCATCGAGCTCGTCGAAGAAGAGGTCCGTGACCTCCTC
GACGAGAACGGCTTCGATCGCGACTGCCCGGTCATCCACACCTCCGCCTACGGCGCCCTGCACGACGACGCTCCGGACCA
CGAGAAGTGGGTCCAGTCCGTCAAGGACCTCATGGCCGCCGTCGATGACTACATCCCAACCCCGGTTCACGACCTGGACA
AGCCGTTCCTGATGCCGATCGAGGACGTCTTCACCATCTCCGGCCGTGGTACCGTCGTCACCGGTCGTGTCGAGCGTGGC
CAGCTGGCCGTCAACACCCCGGTCGAGATCGTCGGCATCCGTCCGACCCAGACCACCACCGTCACCTCCATCGAGACCTT
CCACAAGACCATGGACGCCTGCGAGGCTGGCGACAACACCGGTCTGCTGCTGCGTGGCCTCGGCCGTGAGGATGTCGAGC
GCGGTCAGGTCGTGGCCAAGCCGGGCTCCGTCACCCCGCACACCAAGTTCGAGGGCGAAGTCTACGTGCTGACCAAGGAC
GAAGGCGGCCGTCACTCGCCGTTCTTCTCCAACTACCGTCCGCAGTTCTACTTCCGCACCACCGACGTCACCGGCGTCAT
CGAGCTGCCGGAAGGCGTCGAGATGGTTCAGCCCGGCGACCACGCCACCTTCACCGTTGAGCTGATTCAGCCCATCGCCA
TGGAGGAAGGCCTGACCTTCGCTGTGCGTGAAGGCGGCCACACCGTTGGCTCCGGTCGTGTGACCAAGATCCTCGCCTGA

Upstream 100 bases:

>100_bases
CTCTTCAAAGTGGCACATAACCCAGAAACCCAGTAGAATAAGCGAGTCCCTGTGCGAAAGGGCGCAGGAAACTCACGAGA
CGTCCAGGAGGACAAAAGTA

Downstream 100 bases:

>100_bases
TTTTCATCAGACAAGAATCTTCGCTTGAACTAGCGTTATAGGAAATCCCCTTCGAGGAAACTCAGAGGGGATTTCCTATA
ACCGCAGCAGGTATGGATAT

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MAKEKYERTKPHVNIGTIGHVDHGKTTLTAAISKVLHEEFPDVNPEYDFNQIDSAPEEAARGITINIAHIEYQTEKRHYA
HVDCPGHADFVKNMITGAAQMDGAILVVAATDGPMAQTREHVLLARQVGVPKILVALNKCDMVDDEELIELVEEEVRDLL
DENGFDRDCPVIHTSAYGALHDDAPDHEKWVQSVKDLMAAVDDYIPTPVHDLDKPFLMPIEDVFTISGRGTVVTGRVERG
QLAVNTPVEIVGIRPTQTTTVTSIETFHKTMDACEAGDNTGLLLRGLGREDVERGQVVAKPGSVTPHTKFEGEVYVLTKD
EGGRHSPFFSNYRPQFYFRTTDVTGVIELPEGVEMVQPGDHATFTVELIQPIAMEEGLTFAVREGGHTVGSGRVTKILA

Sequences:

>Translated_399_residues
MAKEKYERTKPHVNIGTIGHVDHGKTTLTAAISKVLHEEFPDVNPEYDFNQIDSAPEEAARGITINIAHIEYQTEKRHYA
HVDCPGHADFVKNMITGAAQMDGAILVVAATDGPMAQTREHVLLARQVGVPKILVALNKCDMVDDEELIELVEEEVRDLL
DENGFDRDCPVIHTSAYGALHDDAPDHEKWVQSVKDLMAAVDDYIPTPVHDLDKPFLMPIEDVFTISGRGTVVTGRVERG
QLAVNTPVEIVGIRPTQTTTVTSIETFHKTMDACEAGDNTGLLLRGLGREDVERGQVVAKPGSVTPHTKFEGEVYVLTKD
EGGRHSPFFSNYRPQFYFRTTDVTGVIELPEGVEMVQPGDHATFTVELIQPIAMEEGLTFAVREGGHTVGSGRVTKILA
>Mature_398_residues
AKEKYERTKPHVNIGTIGHVDHGKTTLTAAISKVLHEEFPDVNPEYDFNQIDSAPEEAARGITINIAHIEYQTEKRHYAH
VDCPGHADFVKNMITGAAQMDGAILVVAATDGPMAQTREHVLLARQVGVPKILVALNKCDMVDDEELIELVEEEVRDLLD
ENGFDRDCPVIHTSAYGALHDDAPDHEKWVQSVKDLMAAVDDYIPTPVHDLDKPFLMPIEDVFTISGRGTVVTGRVERGQ
LAVNTPVEIVGIRPTQTTTVTSIETFHKTMDACEAGDNTGLLLRGLGREDVERGQVVAKPGSVTPHTKFEGEVYVLTKDE
GGRHSPFFSNYRPQFYFRTTDVTGVIELPEGVEMVQPGDHATFTVELIQPIAMEEGLTFAVREGGHTVGSGRVTKILA

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=399, Percent_Identity=55.1378446115288, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI4503471, Length=443, Percent_Identity=30.2483069977427, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI4503475, Length=444, Percent_Identity=29.5045045045045, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI223555963, Length=440, Percent_Identity=28.4090909090909, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI5729864, Length=440, Percent_Identity=28.4090909090909, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI54607086, Length=316, Percent_Identity=27.5316455696203, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI194018522, Length=335, Percent_Identity=28.6567164179104, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI194097354, Length=335, Percent_Identity=28.6567164179104, Blast_Score=118, Evalue=8e-27,
Organism=Homo sapiens, GI194018520, Length=335, Percent_Identity=28.6567164179104, Blast_Score=118, Evalue=8e-27,
Organism=Homo sapiens, GI46094014, Length=331, Percent_Identity=30.8157099697885, Blast_Score=118, Evalue=1e-26,
Organism=Escherichia coli, GI1790412, Length=400, Percent_Identity=67.25, Blast_Score=521, Evalue=1e-149,
Organism=Escherichia coli, GI1789737, Length=400, Percent_Identity=67.25, Blast_Score=521, Evalue=1e-149,
Organism=Escherichia coli, GI2367247, Length=340, Percent_Identity=30.5882352941176, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI48994988, Length=242, Percent_Identity=35.1239669421488, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789108, Length=324, Percent_Identity=26.2345679012346, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=404, Percent_Identity=48.019801980198, Blast_Score=338, Evalue=2e-93,
Organism=Caenorhabditis elegans, GI25141371, Length=377, Percent_Identity=42.1750663129974, Blast_Score=295, Evalue=3e-80,
Organism=Caenorhabditis elegans, GI17552884, Length=336, Percent_Identity=34.2261904761905, Blast_Score=161, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17569207, Length=336, Percent_Identity=34.2261904761905, Blast_Score=161, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI115532067, Length=432, Percent_Identity=28.7037037037037, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI115532065, Length=429, Percent_Identity=28.6713286713287, Blast_Score=131, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI32566303, Length=329, Percent_Identity=31.0030395136778, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17506081, Length=276, Percent_Identity=29.7101449275362, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI32566629, Length=452, Percent_Identity=26.3274336283186, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI32566301, Length=158, Percent_Identity=41.7721518987342, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17557151, Length=229, Percent_Identity=28.3842794759825, Blast_Score=78, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=399, Percent_Identity=59.3984962406015, Blast_Score=462, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6325337, Length=348, Percent_Identity=33.6206896551724, Blast_Score=160, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6319594, Length=348, Percent_Identity=33.6206896551724, Blast_Score=160, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6322937, Length=349, Percent_Identity=27.5071633237822, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320377, Length=338, Percent_Identity=27.810650887574, Blast_Score=93, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6323320, Length=241, Percent_Identity=28.6307053941909, Blast_Score=73, Evalue=8e-14,
Organism=Drosophila melanogaster, GI281363316, Length=397, Percent_Identity=53.6523929471033, Blast_Score=436, Evalue=1e-122,
Organism=Drosophila melanogaster, GI17864358, Length=397, Percent_Identity=53.6523929471033, Blast_Score=436, Evalue=1e-122,
Organism=Drosophila melanogaster, GI19921738, Length=392, Percent_Identity=47.9591836734694, Blast_Score=373, Evalue=1e-103,
Organism=Drosophila melanogaster, GI45553807, Length=358, Percent_Identity=33.2402234636872, Blast_Score=151, Evalue=7e-37,
Organism=Drosophila melanogaster, GI45553816, Length=358, Percent_Identity=33.2402234636872, Blast_Score=151, Evalue=7e-37,
Organism=Drosophila melanogaster, GI24651721, Length=358, Percent_Identity=33.2402234636872, Blast_Score=151, Evalue=7e-37,
Organism=Drosophila melanogaster, GI17864154, Length=358, Percent_Identity=33.2402234636872, Blast_Score=151, Evalue=7e-37,
Organism=Drosophila melanogaster, GI24652838, Length=358, Percent_Identity=33.2402234636872, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI17137572, Length=358, Percent_Identity=33.2402234636872, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI45550900, Length=350, Percent_Identity=30, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI19922690, Length=308, Percent_Identity=28.5714285714286, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI17137380, Length=346, Percent_Identity=30.0578034682081, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24647046, Length=284, Percent_Identity=25.3521126760563, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24647048, Length=284, Percent_Identity=25.3521126760563, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI28572034, Length=156, Percent_Identity=38.4615384615385, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43880; Mature: 43749

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKYERTKPHVNIGTIGHVDHGKTTLTAAISKVLHEEFPDVNPEYDFNQIDSAPEEAA
CCCCHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHH
RGITINIAHIEYQTEKRHYAHVDCPGHADFVKNMITGAAQMDGAILVVAATDGPMAQTRE
CCEEEEEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHH
HVLLARQVGVPKILVALNKCDMVDDEELIELVEEEVRDLLDENGFDRDCPVIHTSAYGAL
HHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCC
HDDAPDHEKWVQSVKDLMAAVDDYIPTPVHDLDKPFLMPIEDVFTISGRGTVVTGRVERG
CCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHEEEECCCCEEEEEEECCC
QLAVNTPVEIVGIRPTQTTTVTSIETFHKTMDACEAGDNTGLLLRGLGREDVERGQVVAK
EEEECCCEEEEEECCCCCEEEHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHCCEEEEC
PGSVTPHTKFEGEVYVLTKDEGGRHSPFFSNYRPQFYFRTTDVTGVIELPEGVEMVQPGD
CCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCEECCCC
HATFTVELIQPIAMEEGLTFAVREGGHTVGSGRVTKILA
CCEEEEEHHHHHHHHCCCEEEEECCCCEECCCCEEEECC
>Mature Secondary Structure 
AKEKYERTKPHVNIGTIGHVDHGKTTLTAAISKVLHEEFPDVNPEYDFNQIDSAPEEAA
CCCHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHH
RGITINIAHIEYQTEKRHYAHVDCPGHADFVKNMITGAAQMDGAILVVAATDGPMAQTRE
CCEEEEEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHH
HVLLARQVGVPKILVALNKCDMVDDEELIELVEEEVRDLLDENGFDRDCPVIHTSAYGAL
HHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCC
HDDAPDHEKWVQSVKDLMAAVDDYIPTPVHDLDKPFLMPIEDVFTISGRGTVVTGRVERG
CCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHEEEECCCCEEEEEEECCC
QLAVNTPVEIVGIRPTQTTTVTSIETFHKTMDACEAGDNTGLLLRGLGREDVERGQVVAK
EEEECCCEEEEEECCCCCEEEHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHCCEEEEC
PGSVTPHTKFEGEVYVLTKDEGGRHSPFFSNYRPQFYFRTTDVTGVIELPEGVEMVQPGD
CCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCEECCCC
HATFTVELIQPIAMEEGLTFAVREGGHTVGSGRVTKILA
CCEEEEEHHHHHHHHCCCEEEEECCCCEECCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA