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The map label for this gene is pdhD [H]

Identifier: 213692757

GI number: 213692757

Start: 2062552

End: 2064042

Strand: Direct

Name: pdhD [H]

Synonym: Blon_1891

Alternate gene names: 213692757

Gene position: 2062552-2064042 (Clockwise)

Preceding gene: 213692756

Following gene: 213692758

Centisome position: 72.81

GC content: 64.92

Gene sequence:

>1491_bases
ATGAGCGAACAGTTTGATCTGGTCATCATCGGGGCCGGCCCCGGCGGCTATTCCACAGCTCTGCGCGCGGCCGAACTCGG
CATGAAAGTGGCACTCGTCGAGCGTGACGCCACCGTGGGCGGCACATGTCTCAACCGTGGCTGCATCCCCTCCAAGGCGC
TCATCACCGCCACCCACACCATCGACACCGTGCACCGTGCAGCCGAACTCGGCGTCAACGCCTCGGTGAATGGCATCGAC
TTCGGCACGCTGCGCGACTATCGCCTGCGCGTGGTCAAGACGATGGTGGGAGGACTGGCCGGACTGCTCGCCCATCGCGG
CATCACCGTGTTCCGGGCCAATGCCGCGTTCCATGCGGATGAGATTGCTCCAGCAACTTCCAACCACATCGTCCGCCTCG
TCCCCTCCCCCGGCCAAAGCGACATCCTCACCTATCACAAGGCCGATGTCCCCGAACCCAGCGGCCCAACGATGGACCTC
ACGGCCATGAACATCGTCATCGCCACCGGTGCCAAGCCACGCCCATTGCCCGGCAACCCGTTCGCCGGCGCCCTCATCGA
CTCCACACAGGCGCTGGAAGTCAACGAATTTCCCTCTTCGGCGGTGATTATCGGCGCCGGGGCCATCGCCCTTGAATTCG
CCTCGATATGGAACGCCACCGGTTCGAAGGTGACCCTGCTGATTCGCAAGGACCGCGTGCTCTCAGCCTGGGACCGGAGA
GCCGGCACCACGCTGACCCGTGAGCTCAAACGTCATGGCGTGAACATCATCACCCGCGCCAGCGTCACCCACGTGGACAC
CGGCGCAAACCTTGGCGCCACCGTGCACTACACGCGCGAAGGCCAGGATGGCGAACAGAGCGTCTGGGGCGAAATCGCCC
TTGTCGCCATCGGGCGTGACCCGATCACCGACCCAGCATGGGGCGTGACGATCGACGACCACGGACATGTCGCCACGGAT
GCGTACGGTCGCACCGACAAACCGGGCGTATGGGCGGTAGGCGACGTGACGGCCGGTCACGCGCTGGCGCATCGTGCTTT
TGAGCAAGGCATCGTCATCGCGGAGACCATCGCCGGACTCAATCCCAAGCCGGTGGACGAAGCCACCGTGCCGCAGATCG
TGTTCTCCTTCCCCGAAGCGGCGTCGGTCGGGCTCACCGTCGAACAGGCGCAGGCGAGGGAGGATCTCATTGAAATCAAG
GAGACCAACTATCCGATGCTGGCGAACGCGCGCATGCTGATGAGCGGCACGGCCGGCTCATTGACCATCGTCTCCGGCTG
CGACGCGGCCAACCCGGACACCCCGCGCGTGCTCGGCGTGCACATGGTCTCCCCGATGGCCTCCGACATCATCGCCGAAG
CCGAACAGCTGGTCGGCAACCATGTGCCGCTCGCCGACGCAGCCCGCCTCGTTCACCCGCATCCCACATTCAGTGAAACG
CTCGGAGAGGCGCTGCTCAAGGCCGACGGACGACCATTGCACACACGCTGA

Upstream 100 bases:

>100_bases
GAAAACAGTCCAGTGGACTGTTCTTAGGCGAAGTGGCGGCGATAGCCGCATATGGCTCCCTAGCATGGGAGCCATACTTA
TATGCATCAAGGAGGCAACC

Downstream 100 bases:

>100_bases
GACCAACCAGCACAAGCAAGTTCGGACCAGCGCACCTGATAAGATATTGCCCAGTGTGTTTCGTCCCAACAAAGGAAGAA
GTGATGGCGGAGAAGGACGC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex [H]

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MSEQFDLVIIGAGPGGYSTALRAAELGMKVALVERDATVGGTCLNRGCIPSKALITATHTIDTVHRAAELGVNASVNGID
FGTLRDYRLRVVKTMVGGLAGLLAHRGITVFRANAAFHADEIAPATSNHIVRLVPSPGQSDILTYHKADVPEPSGPTMDL
TAMNIVIATGAKPRPLPGNPFAGALIDSTQALEVNEFPSSAVIIGAGAIALEFASIWNATGSKVTLLIRKDRVLSAWDRR
AGTTLTRELKRHGVNIITRASVTHVDTGANLGATVHYTREGQDGEQSVWGEIALVAIGRDPITDPAWGVTIDDHGHVATD
AYGRTDKPGVWAVGDVTAGHALAHRAFEQGIVIAETIAGLNPKPVDEATVPQIVFSFPEAASVGLTVEQAQAREDLIEIK
ETNYPMLANARMLMSGTAGSLTIVSGCDAANPDTPRVLGVHMVSPMASDIIAEAEQLVGNHVPLADAARLVHPHPTFSET
LGEALLKADGRPLHTR

Sequences:

>Translated_496_residues
MSEQFDLVIIGAGPGGYSTALRAAELGMKVALVERDATVGGTCLNRGCIPSKALITATHTIDTVHRAAELGVNASVNGID
FGTLRDYRLRVVKTMVGGLAGLLAHRGITVFRANAAFHADEIAPATSNHIVRLVPSPGQSDILTYHKADVPEPSGPTMDL
TAMNIVIATGAKPRPLPGNPFAGALIDSTQALEVNEFPSSAVIIGAGAIALEFASIWNATGSKVTLLIRKDRVLSAWDRR
AGTTLTRELKRHGVNIITRASVTHVDTGANLGATVHYTREGQDGEQSVWGEIALVAIGRDPITDPAWGVTIDDHGHVATD
AYGRTDKPGVWAVGDVTAGHALAHRAFEQGIVIAETIAGLNPKPVDEATVPQIVFSFPEAASVGLTVEQAQAREDLIEIK
ETNYPMLANARMLMSGTAGSLTIVSGCDAANPDTPRVLGVHMVSPMASDIIAEAEQLVGNHVPLADAARLVHPHPTFSET
LGEALLKADGRPLHTR
>Mature_495_residues
SEQFDLVIIGAGPGGYSTALRAAELGMKVALVERDATVGGTCLNRGCIPSKALITATHTIDTVHRAAELGVNASVNGIDF
GTLRDYRLRVVKTMVGGLAGLLAHRGITVFRANAAFHADEIAPATSNHIVRLVPSPGQSDILTYHKADVPEPSGPTMDLT
AMNIVIATGAKPRPLPGNPFAGALIDSTQALEVNEFPSSAVIIGAGAIALEFASIWNATGSKVTLLIRKDRVLSAWDRRA
GTTLTRELKRHGVNIITRASVTHVDTGANLGATVHYTREGQDGEQSVWGEIALVAIGRDPITDPAWGVTIDDHGHVATDA
YGRTDKPGVWAVGDVTAGHALAHRAFEQGIVIAETIAGLNPKPVDEATVPQIVFSFPEAASVGLTVEQAQAREDLIEIKE
TNYPMLANARMLMSGTAGSLTIVSGCDAANPDTPRVLGVHMVSPMASDIIAEAEQLVGNHVPLADAARLVHPHPTFSETL
GEALLKADGRPLHTR

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=492, Percent_Identity=31.9105691056911, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI50301238, Length=498, Percent_Identity=30.1204819277108, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI291045266, Length=459, Percent_Identity=25.4901960784314, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI22035672, Length=485, Percent_Identity=26.8041237113402, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI148277071, Length=437, Percent_Identity=24.4851258581236, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI148277065, Length=437, Percent_Identity=24.4851258581236, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI33519430, Length=437, Percent_Identity=24.4851258581236, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI33519428, Length=437, Percent_Identity=24.4851258581236, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI33519426, Length=437, Percent_Identity=24.4851258581236, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI291045268, Length=361, Percent_Identity=26.0387811634349, Blast_Score=79, Evalue=7e-15,
Organism=Escherichia coli, GI1786307, Length=484, Percent_Identity=30.5785123966942, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI87082354, Length=455, Percent_Identity=26.5934065934066, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87081717, Length=489, Percent_Identity=27.8118609406953, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1789915, Length=426, Percent_Identity=28.6384976525822, Blast_Score=130, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI32565766, Length=492, Percent_Identity=33.130081300813, Blast_Score=238, Evalue=7e-63,
Organism=Caenorhabditis elegans, GI71983429, Length=426, Percent_Identity=27.9342723004695, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71983419, Length=426, Percent_Identity=27.9342723004695, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17557007, Length=504, Percent_Identity=26.5873015873016, Blast_Score=122, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6321091, Length=508, Percent_Identity=31.2992125984252, Blast_Score=214, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6325166, Length=502, Percent_Identity=25.0996015936255, Blast_Score=133, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6325240, Length=499, Percent_Identity=24.0480961923848, Blast_Score=121, Evalue=2e-28,
Organism=Drosophila melanogaster, GI21358499, Length=506, Percent_Identity=31.8181818181818, Blast_Score=234, Evalue=8e-62,
Organism=Drosophila melanogaster, GI17737741, Length=508, Percent_Identity=24.8031496062992, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24640551, Length=503, Percent_Identity=24.8508946322068, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24640553, Length=503, Percent_Identity=24.8508946322068, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24640549, Length=503, Percent_Identity=24.8508946322068, Blast_Score=99, Evalue=6e-21,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 52141; Mature: 52010

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQFDLVIIGAGPGGYSTALRAAELGMKVALVERDATVGGTCLNRGCIPSKALITATHT
CCCCEEEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCCCHHHCCCCCCCCEEEEEEHH
IDTVHRAAELGVNASVNGIDFGTLRDYRLRVVKTMVGGLAGLLAHRGITVFRANAAFHAD
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHH
EIAPATSNHIVRLVPSPGQSDILTYHKADVPEPSGPTMDLTAMNIVIATGAKPRPLPGNP
HCCCCCCCCEEEEECCCCCCCEEEEECCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCC
FAGALIDSTQALEVNEFPSSAVIIGAGAIALEFASIWNATGSKVTLLIRKDRVLSAWDRR
CCCCEECCCCEEEECCCCCCEEEEECCHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHH
AGTTLTRELKRHGVNIITRASVTHVDTGANLGATVHYTREGQDGEQSVWGEIALVAIGRD
CCHHHHHHHHHCCCCEEEECEEEEECCCCCCCEEEEEECCCCCCCHHHCCEEEEEEECCC
PITDPAWGVTIDDHGHVATDAYGRTDKPGVWAVGDVTAGHALAHRAFEQGIVIAETIAGL
CCCCCCCEEEECCCCCEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHCCEEEEHHHCCC
NPKPVDEATVPQIVFSFPEAASVGLTVEQAQAREDLIEIKETNYPMLANARMLMSGTAGS
CCCCCCCCHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCEEECCEEEEECCCCC
LTIVSGCDAANPDTPRVLGVHMVSPMASDIIAEAEQLVGNHVPLADAARLVHPHPTFSET
EEEEECCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHH
LGEALLKADGRPLHTR
HHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SEQFDLVIIGAGPGGYSTALRAAELGMKVALVERDATVGGTCLNRGCIPSKALITATHT
CCCEEEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCCCHHHCCCCCCCCEEEEEEHH
IDTVHRAAELGVNASVNGIDFGTLRDYRLRVVKTMVGGLAGLLAHRGITVFRANAAFHAD
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHH
EIAPATSNHIVRLVPSPGQSDILTYHKADVPEPSGPTMDLTAMNIVIATGAKPRPLPGNP
HCCCCCCCCEEEEECCCCCCCEEEEECCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCC
FAGALIDSTQALEVNEFPSSAVIIGAGAIALEFASIWNATGSKVTLLIRKDRVLSAWDRR
CCCCEECCCCEEEECCCCCCEEEEECCHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHH
AGTTLTRELKRHGVNIITRASVTHVDTGANLGATVHYTREGQDGEQSVWGEIALVAIGRD
CCHHHHHHHHHCCCCEEEECEEEEECCCCCCCEEEEEECCCCCCCHHHCCEEEEEEECCC
PITDPAWGVTIDDHGHVATDAYGRTDKPGVWAVGDVTAGHALAHRAFEQGIVIAETIAGL
CCCCCCCEEEECCCCCEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHCCEEEEHHHCCC
NPKPVDEATVPQIVFSFPEAASVGLTVEQAQAREDLIEIKETNYPMLANARMLMSGTAGS
CCCCCCCCHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCEEECCEEEEECCCCC
LTIVSGCDAANPDTPRVLGVHMVSPMASDIIAEAEQLVGNHVPLADAARLVHPHPTFSET
EEEEECCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHH
LGEALLKADGRPLHTR
HHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2200674; 2253629; 6896188; 8805537 [H]