Click here to switch to the map view.

The map label for this gene is greA [H]

Identifier: 213692643

GI number: 213692643

Start: 1952817

End: 1953296

Strand: Reverse

Name: greA [H]

Synonym: Blon_1776

Alternate gene names: 213692643

Gene position: 1953296-1952817 (Counterclockwise)

Preceding gene: 213692644

Following gene: 213692641

Centisome position: 68.95

GC content: 63.12

Gene sequence:

>480_bases
ATGGCTGAGGACAAGGTCTACCTGCTGACGCAGGAAGCATACAACAAGATGAAGGAAGAGCTCGCCCATCGTGAAGGTGA
GCTGCGTGAGGAAATCGCGCACAAGATCGCCGTGGCGCGTGCCGAGGGCGACTTGTCCGAGAACGGCGGCTACCAGGCCG
CCCGTGAGGCGCAGAGCAAGAACGAGGGCCGCATCACCGAGCTCACCGTCAAGCTGCGCGATTCCAAGATCATCGAGAAT
GCTCCGGAAGGCACCGTGGGCGAGGGCTCTGTGGTGACCATCGAGCTCAACGGCAACGAGATGACCTACGTGCTCGGCAG
CCGTGACATCGTGGCCGGCACCGACTACGACATCATGACCCCGGATTCCCCGATTGGCAAGGCTATCGAGGGTGCCAAGG
TGGGCGACGTCGTCTCCTACACCGCACCGAACGGCCGCGAGCTGTCCGTGACGGTCAAGGCCGCCAAGCCGCTGGCCTGA

Upstream 100 bases:

>100_bases
GACCACAGGTCAGCACATGACGAACGCCGGGGTCATAGCCGGCGTTTTGTCATAAATAACGAAGATTGTTTCAGCATCGA
CAACGGTTCAGGAGGACGAT

Downstream 100 bases:

>100_bases
CGTCGTCTCAACGCGAGGGAGGGGTTCCGGACGATTCCGGAACCCCTCCCCGTTCGTTGGCCCGGCACCGTCGCCGGGCC
TGGAGACGTCAATGCCACAG

Product: GreA/GreB family elongation factor

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MAEDKVYLLTQEAYNKMKEELAHREGELREEIAHKIAVARAEGDLSENGGYQAAREAQSKNEGRITELTVKLRDSKIIEN
APEGTVGEGSVVTIELNGNEMTYVLGSRDIVAGTDYDIMTPDSPIGKAIEGAKVGDVVSYTAPNGRELSVTVKAAKPLA

Sequences:

>Translated_159_residues
MAEDKVYLLTQEAYNKMKEELAHREGELREEIAHKIAVARAEGDLSENGGYQAAREAQSKNEGRITELTVKLRDSKIIEN
APEGTVGEGSVVTIELNGNEMTYVLGSRDIVAGTDYDIMTPDSPIGKAIEGAKVGDVVSYTAPNGRELSVTVKAAKPLA
>Mature_158_residues
AEDKVYLLTQEAYNKMKEELAHREGELREEIAHKIAVARAEGDLSENGGYQAAREAQSKNEGRITELTVKLRDSKIIENA
PEGTVGEGSVVTIELNGNEMTYVLGSRDIVAGTDYDIMTPDSPIGKAIEGAKVGDVVSYTAPNGRELSVTVKAAKPLA

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=152, Percent_Identity=34.8684210526316, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI87082225, Length=143, Percent_Identity=36.3636363636364, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 17135; Mature: 17004

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00829 GREAB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEDKVYLLTQEAYNKMKEELAHREGELREEIAHKIAVARAEGDLSENGGYQAAREAQSK
CCCCEEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCC
NEGRITELTVKLRDSKIIENAPEGTVGEGSVVTIELNGNEMTYVLGSRDIVAGTDYDIMT
CCCCEEEEEEEECCCCHHCCCCCCCCCCCCEEEEEECCCEEEEEECCCEEECCCCCEEEC
PDSPIGKAIEGAKVGDVVSYTAPNGRELSVTVKAAKPLA
CCCCCCHHCCCCCCCCEEEEECCCCCEEEEEEECCCCCC
>Mature Secondary Structure 
AEDKVYLLTQEAYNKMKEELAHREGELREEIAHKIAVARAEGDLSENGGYQAAREAQSK
CCCEEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCC
NEGRITELTVKLRDSKIIENAPEGTVGEGSVVTIELNGNEMTYVLGSRDIVAGTDYDIMT
CCCCEEEEEEEECCCCHHCCCCCCCCCCCCEEEEEECCCEEEEEECCCEEECCCCCEEEC
PDSPIGKAIEGAKVGDVVSYTAPNGRELSVTVKAAKPLA
CCCCCCHHCCCCCCCCEEEEECCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12692562; 11572948 [H]