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The map label for this gene is ispF

Identifier: 213692626

GI number: 213692626

Start: 1932998

End: 1933522

Strand: Reverse

Name: ispF

Synonym: Blon_1759

Alternate gene names: 213692626

Gene position: 1933522-1932998 (Counterclockwise)

Preceding gene: 213692627

Following gene: 213692622

Centisome position: 68.26

GC content: 61.71

Gene sequence:

>525_bases
ATGGGGGCTGCAGGAGTGCTGATAGGGCAAGGTTTTGACGCTCACCGTTTTGCGCCTGCCGGTTCAGGCCGTGAACTGTG
GATTGCCGGGCTCTACTGGCCGGTGCCTGATTCATGCAGCGAGGCCGATGCCGCCAGATACGAGGGTATCGAAGGCGATT
CCGATGGCGATGTGGCCGCGCACGCCCTGATTGACGCGTTGCTTGCCGCCGCCCGATTGGGAGACATTGGTTCACTGTTC
GGTGTCGGTGCGGACGCGCATGGTGCCGGAAGGCATGGCATCGATATGCTGCAAGAAGTTGTGGCGCATCTGGCATCCAA
CGGGTATACGCCGGCATCGGCCTCAGTGGCGATCATCGGCAACCGTCCGAAAATCGGCACACGCCGTGCTGAAGCCGAAG
CCGCCCTGTCTGCGGCGGTTGGTTGCCCGGTATCGGTTACCGCCACCACCACCGACCATATGGGATTCACTGGCCGCGGC
GAAGGCATCGCCGCCATCGCCAATGCCTTGGTGGAAAAGATATAA

Upstream 100 bases:

>100_bases
TTTTGAGGCCTCGGTCGTGTCCTTATGCGATGCGCCGGGGCTTCGTGATTCATAATCGTTTGCTGCCGTTCATCTGGGCA
ATGCCATCGAAGGGAACTGC

Downstream 100 bases:

>100_bases
CAAAGCCCCCGCTATGAATTTGCCAGCGGGGGCAAGAAAAATGAATGAGATTACTTCCGCACTGTCTCCACGGCCTTGGC
GATAGCCCAGAACAGGGTGG

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 174; Mature: 173

Protein sequence:

>174_residues
MGAAGVLIGQGFDAHRFAPAGSGRELWIAGLYWPVPDSCSEADAARYEGIEGDSDGDVAAHALIDALLAAARLGDIGSLF
GVGADAHGAGRHGIDMLQEVVAHLASNGYTPASASVAIIGNRPKIGTRRAEAEAALSAAVGCPVSVTATTTDHMGFTGRG
EGIAAIANALVEKI

Sequences:

>Translated_174_residues
MGAAGVLIGQGFDAHRFAPAGSGRELWIAGLYWPVPDSCSEADAARYEGIEGDSDGDVAAHALIDALLAAARLGDIGSLF
GVGADAHGAGRHGIDMLQEVVAHLASNGYTPASASVAIIGNRPKIGTRRAEAEAALSAAVGCPVSVTATTTDHMGFTGRG
EGIAAIANALVEKI
>Mature_173_residues
GAAGVLIGQGFDAHRFAPAGSGRELWIAGLYWPVPDSCSEADAARYEGIEGDSDGDVAAHALIDALLAAARLGDIGSLFG
VGADAHGAGRHGIDMLQEVVAHLASNGYTPASASVAIIGNRPKIGTRRAEAEAALSAAVGCPVSVTATTTDHMGFTGRGE
GIAAIANALVEKI

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=169, Percent_Identity=39.0532544378698, Blast_Score=91, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_BIFLI (B7GT04)

Other databases:

- EMBL:   CP001095
- RefSeq:   YP_002323212.1
- GeneID:   7054401
- GenomeReviews:   CP001095_GR
- KEGG:   bln:Blon_1759
- HOGENOM:   HBG630070
- ProtClustDB:   PRK00084
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 17462; Mature: 17330

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAAGVLIGQGFDAHRFAPAGSGRELWIAGLYWPVPDSCSEADAARYEGIEGDSDGDVAA
CCCCCEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHCCCCCCCCCHHHH
HALIDALLAAARLGDIGSLFGVGADAHGAGRHGIDMLQEVVAHLASNGYTPASASVAIIG
HHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEC
NRPKIGTRRAEAEAALSAAVGCPVSVTATTTDHMGFTGRGEGIAAIANALVEKI
CCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
GAAGVLIGQGFDAHRFAPAGSGRELWIAGLYWPVPDSCSEADAARYEGIEGDSDGDVAA
CCCCEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHCCCCCCCCCHHHH
HALIDALLAAARLGDIGSLFGVGADAHGAGRHGIDMLQEVVAHLASNGYTPASASVAIIG
HHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEC
NRPKIGTRRAEAEAALSAAVGCPVSVTATTTDHMGFTGRGEGIAAIANALVEKI
CCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA