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The map label for this gene is folD [H]

Identifier: 213692622

GI number: 213692622

Start: 1928825

End: 1929700

Strand: Reverse

Name: folD [H]

Synonym: Blon_1755

Alternate gene names: 213692622

Gene position: 1929700-1928825 (Counterclockwise)

Preceding gene: 213692626

Following gene: 213692621

Centisome position: 68.12

GC content: 63.01

Gene sequence:

>876_bases
ATGTCCACAAAAATCGACGGTAAGCAGATTGCGGCCGAAATCAAGACCAACCTTGCCGAGCGAGTGAACAGGCTCAAGGC
GCAGGGCATCCAACCAGGGCTGGGCACGCTGCTGGTGGGCGAAGACCCCGGGTCGATGAAGTACGTGGCCGGCAAGCACG
CCGATTGTCAGGAAGTGGGCATTACCTCAGTCAAGAAGGAACTGCCGGCGGACGCCAGCTTCGACGACATCGCCGCTACC
GTACGCGAACTCAACGAAGATCCGGCCTGCACCGGGTTCATCGTCCAGTTGCCGTTGCCCAAGGGCATCAACGAGAACGC
GATCATCGACATGATCGACCCGGCCAAGGATGCCGACGGCATGCACCCGTACAACCTCGGCGAACTGGTGCTGCATGTGC
GCGGCGACATTTCGACACCGCTGCCTTGCACTCCGCGCGGTGTGCTCGAGCTGCTCGACGCCTATGACATTGATCTGAAC
GGCAAGGAAGTGTGCGTGCTTGGCCGCGGCATCACCATTGGCCGCACGATTGGCCTGATGCTCACGCGCAACGCCGTCAA
CGCCACCGTCACCCTGTGCCACACCGGTACCCGGGATGTGGCCGACCACATGCGCCGCGCCGACGTGATCGTCGCCGCAA
TGGGTTCGGCCGGATTCGTGACGCCCGACAAGATCAAGGACGGCGCCGTGCTGGTCGACGTGGGCATCAGCCGCGTCTAC
GACGAAGAAGCCGGTCGCTACCGCATCAAGGGCGACGTGGACAAGGCCTGCTATGACAAGGCATCCGCCTACACCCCCAA
CCCCGGAGGCGTGGGCCCTATGACCCGAGCCATGCTCCTGGCCAACGTCGTGGAAATGGCCGAACGACACGCCTAG

Upstream 100 bases:

>100_bases
ACGCCTTCTTCGTGAGAGCGAACGATATATAATCCTGACTTGTTCCAACGATAGCCTATCGTTTCGATAGAATGCACCGA
AACTACAGGGGAGGGCGTTG

Downstream 100 bases:

>100_bases
AGTTTCCTAGGAAATTTGGGCAGAGTTGCTTGTATTGTGAATATCCGTATGCCTGTAAGGGTGTGCGTATAACTGAATAT
CGAGAAATTATCCATCCACT

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MSTKIDGKQIAAEIKTNLAERVNRLKAQGIQPGLGTLLVGEDPGSMKYVAGKHADCQEVGITSVKKELPADASFDDIAAT
VRELNEDPACTGFIVQLPLPKGINENAIIDMIDPAKDADGMHPYNLGELVLHVRGDISTPLPCTPRGVLELLDAYDIDLN
GKEVCVLGRGITIGRTIGLMLTRNAVNATVTLCHTGTRDVADHMRRADVIVAAMGSAGFVTPDKIKDGAVLVDVGISRVY
DEEAGRYRIKGDVDKACYDKASAYTPNPGGVGPMTRAMLLANVVEMAERHA

Sequences:

>Translated_291_residues
MSTKIDGKQIAAEIKTNLAERVNRLKAQGIQPGLGTLLVGEDPGSMKYVAGKHADCQEVGITSVKKELPADASFDDIAAT
VRELNEDPACTGFIVQLPLPKGINENAIIDMIDPAKDADGMHPYNLGELVLHVRGDISTPLPCTPRGVLELLDAYDIDLN
GKEVCVLGRGITIGRTIGLMLTRNAVNATVTLCHTGTRDVADHMRRADVIVAAMGSAGFVTPDKIKDGAVLVDVGISRVY
DEEAGRYRIKGDVDKACYDKASAYTPNPGGVGPMTRAMLLANVVEMAERHA
>Mature_290_residues
STKIDGKQIAAEIKTNLAERVNRLKAQGIQPGLGTLLVGEDPGSMKYVAGKHADCQEVGITSVKKELPADASFDDIAATV
RELNEDPACTGFIVQLPLPKGINENAIIDMIDPAKDADGMHPYNLGELVLHVRGDISTPLPCTPRGVLELLDAYDIDLNG
KEVCVLGRGITIGRTIGLMLTRNAVNATVTLCHTGTRDVADHMRRADVIVAAMGSAGFVTPDKIKDGAVLVDVGISRVYD
EEAGRYRIKGDVDKACYDKASAYTPNPGGVGPMTRAMLLANVVEMAERHA

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=294, Percent_Identity=38.4353741496599, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI94721354, Length=295, Percent_Identity=37.9661016949153, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI222418558, Length=295, Percent_Identity=37.2881355932203, Blast_Score=170, Evalue=1e-42,
Organism=Escherichia coli, GI1786741, Length=283, Percent_Identity=39.9293286219081, Blast_Score=196, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17568735, Length=294, Percent_Identity=35.0340136054422, Blast_Score=149, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6319558, Length=291, Percent_Identity=37.8006872852234, Blast_Score=192, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6321643, Length=301, Percent_Identity=35.5481727574751, Blast_Score=172, Evalue=5e-44,
Organism=Drosophila melanogaster, GI17136816, Length=300, Percent_Identity=41.3333333333333, Blast_Score=225, Evalue=2e-59,
Organism=Drosophila melanogaster, GI17136818, Length=300, Percent_Identity=41.3333333333333, Blast_Score=225, Evalue=3e-59,
Organism=Drosophila melanogaster, GI62472483, Length=292, Percent_Identity=37.6712328767123, Blast_Score=164, Evalue=7e-41,
Organism=Drosophila melanogaster, GI45551871, Length=292, Percent_Identity=37.6712328767123, Blast_Score=164, Evalue=7e-41,
Organism=Drosophila melanogaster, GI24645718, Length=292, Percent_Identity=37.6712328767123, Blast_Score=164, Evalue=8e-41,
Organism=Drosophila melanogaster, GI17137370, Length=292, Percent_Identity=37.6712328767123, Blast_Score=164, Evalue=8e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 31003; Mature: 30872

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTKIDGKQIAAEIKTNLAERVNRLKAQGIQPGLGTLLVGEDPGSMKYVAGKHADCQEVG
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEECCCCCCHHHHC
ITSVKKELPADASFDDIAATVRELNEDPACTGFIVQLPLPKGINENAIIDMIDPAKDADG
HHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCCC
MHPYNLGELVLHVRGDISTPLPCTPRGVLELLDAYDIDLNGKEVCVLGRGITIGRTIGLM
CCCCCHHHEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCEEEHHHEEEE
LTRNAVNATVTLCHTGTRDVADHMRRADVIVAAMGSAGFVTPDKIKDGAVLVDVGISRVY
EECCCCCEEEEEEECCCHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCEEEEECCHHHHH
DEEAGRYRIKGDVDKACYDKASAYTPNPGGVGPMTRAMLLANVVEMAERHA
HHCCCCEEECCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STKIDGKQIAAEIKTNLAERVNRLKAQGIQPGLGTLLVGEDPGSMKYVAGKHADCQEVG
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEECCCCCCHHHHC
ITSVKKELPADASFDDIAATVRELNEDPACTGFIVQLPLPKGINENAIIDMIDPAKDADG
HHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCCC
MHPYNLGELVLHVRGDISTPLPCTPRGVLELLDAYDIDLNGKEVCVLGRGITIGRTIGLM
CCCCCHHHEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCEEEHHHEEEE
LTRNAVNATVTLCHTGTRDVADHMRRADVIVAAMGSAGFVTPDKIKDGAVLVDVGISRVY
EECCCCCEEEEEEECCCHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCEEEEECCHHHHH
DEEAGRYRIKGDVDKACYDKASAYTPNPGGVGPMTRAMLLANVVEMAERHA
HHCCCCEEECCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12381787 [H]