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The map label for this gene is yfiK [H]

Identifier: 213692504

GI number: 213692504

Start: 1785950

End: 1786603

Strand: Direct

Name: yfiK [H]

Synonym: Blon_1633

Alternate gene names: 213692504

Gene position: 1785950-1786603 (Clockwise)

Preceding gene: 213692503

Following gene: 213692507

Centisome position: 63.05

GC content: 63.15

Gene sequence:

>654_bases
ATGAGAATCGCAATCGTGGATGATGATCCGATCGTCTGCCAGTCGTTGGAGACGATTCTGACGGCCACCGGCACGGCCGA
GGTGCTATGGACGGCAAATGACGGCGATACCGCCGTGCGCCGTTATTTCGAGACGCCGGCGAGCCGACCGGACGTGCTGC
TGATCGATATTCAGATGCCCGGCACCAGCGGTTTGGATGCCGCGCGCGATATTCTGGCCACGGACCCGGCCGCGCGCATA
CTGTTCCTGACCACGTTTGCGGACCAGTCGTATATCGCGCAGGCGATGGGGCTTGGCGCCAAGGGCTATCTCATCAAACA
GGATGTGGCGGCGGTCGGGCCTGCGCTGCAGGCGGTGATGGCAGGTCAGGTGGTGCTCGGCGCGGAAGTGCTCGGCAAGC
TCACCGAACGGACTCCGGATCCTGCCGATTCCGACGATTCCGGAGATACCGCCGATTCCATCGAAGGCCTGCTTGGCGAG
CGTGAGCGTGAGATCACCGCGCTGGTCGCCGAGGGCCTCGATAACCGCGACATCGCCGCCAGACTGTTCCTGAGCGAAGG
CACGGTCCGCAATCGCATCAGCGCGATACTGGACAAGCTCGGTCTGACCAATCGCACCCAACTCGCCATCCTCTGGCTCA
ACGCCCATCGGTGA

Upstream 100 bases:

>100_bases
GCGCCCTTGGCGGCACTGCGGTTTGCGGGCCGTATGAGGGCGGCTGGCGTGTGTTCGTATCCGTGCCGAAGAAGCGTTGG
ACCATGGAATCGAGGGATTG

Downstream 100 bases:

>100_bases
GGGGCCTTTGGTCGAGTAGGTCTCTCCCTCCGTCATCGCTTCGCGATGCCACCTCGAACGCGATTCAGGACAGCTTGCAG
CTGACCTCTTATTCGCTCGT

Product: two component transcriptional regulator, LuxR family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MRIAIVDDDPIVCQSLETILTATGTAEVLWTANDGDTAVRRYFETPASRPDVLLIDIQMPGTSGLDAARDILATDPAARI
LFLTTFADQSYIAQAMGLGAKGYLIKQDVAAVGPALQAVMAGQVVLGAEVLGKLTERTPDPADSDDSGDTADSIEGLLGE
REREITALVAEGLDNRDIAARLFLSEGTVRNRISAILDKLGLTNRTQLAILWLNAHR

Sequences:

>Translated_217_residues
MRIAIVDDDPIVCQSLETILTATGTAEVLWTANDGDTAVRRYFETPASRPDVLLIDIQMPGTSGLDAARDILATDPAARI
LFLTTFADQSYIAQAMGLGAKGYLIKQDVAAVGPALQAVMAGQVVLGAEVLGKLTERTPDPADSDDSGDTADSIEGLLGE
REREITALVAEGLDNRDIAARLFLSEGTVRNRISAILDKLGLTNRTQLAILWLNAHR
>Mature_217_residues
MRIAIVDDDPIVCQSLETILTATGTAEVLWTANDGDTAVRRYFETPASRPDVLLIDIQMPGTSGLDAARDILATDPAARI
LFLTTFADQSYIAQAMGLGAKGYLIKQDVAAVGPALQAVMAGQVVLGAEVLGKLTERTPDPADSDDSGDTADSIEGLLGE
REREITALVAEGLDNRDIAARLFLSEGTVRNRISAILDKLGLTNRTQLAILWLNAHR

Specific function: Member of the two-component regulatory system yfiJ/yfiK [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786747, Length=208, Percent_Identity=27.8846153846154, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1788521, Length=217, Percent_Identity=31.7972350230415, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788222, Length=217, Percent_Identity=29.4930875576037, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1788712, Length=201, Percent_Identity=23.3830845771144, Blast_Score=64, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23160; Mature: 23160

Theoretical pI: Translated: 4.20; Mature: 4.20

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAIVDDDPIVCQSLETILTATGTAEVLWTANDGDTAVRRYFETPASRPDVLLIDIQMP
CEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCCEEEEEEECC
GTSGLDAARDILATDPAARILFLTTFADQSYIAQAMGLGAKGYLIKQDVAAVGPALQAVM
CCCCCHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHH
AGQVVLGAEVLGKLTERTPDPADSDDSGDTADSIEGLLGEREREITALVAEGLDNRDIAA
HCHHHCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCEE
RLFLSEGTVRNRISAILDKLGLTNRTQLAILWLNAHR
EEEECCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCC
>Mature Secondary Structure
MRIAIVDDDPIVCQSLETILTATGTAEVLWTANDGDTAVRRYFETPASRPDVLLIDIQMP
CEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCCEEEEEEECC
GTSGLDAARDILATDPAARILFLTTFADQSYIAQAMGLGAKGYLIKQDVAAVGPALQAVM
CCCCCHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHH
AGQVVLGAEVLGKLTERTPDPADSDDSGDTADSIEGLLGEREREITALVAEGLDNRDIAA
HCHHHCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCEE
RLFLSEGTVRNRISAILDKLGLTNRTQLAILWLNAHR
EEEECCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8973323; 9384377 [H]