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The map label for this gene is insQ [H]

Identifier: 213692077

GI number: 213692077

Start: 1379394

End: 1380851

Strand: Reverse

Name: insQ [H]

Synonym: Blon_1195

Alternate gene names: 213692077

Gene position: 1380851-1379394 (Counterclockwise)

Preceding gene: 213692079

Following gene: 213692076

Centisome position: 48.75

GC content: 61.52

Gene sequence:

>1458_bases
ATGAGCCAGAAGGTCCGCATCGACGGCGTCAAGCACGTTGGCGCGAGCGTCTTCCTGGGAGTGGACCACTGCGGCAATCC
CTGTTGGACCCGTAATCCCGAGCGCGTCATGGTATGGCTGTGCGACGGATGGCGCACCCGATTCAACCAAAGGCGCGAGC
AGCGCCCGCACAACGTGCCCGTCAAGGACGAGGCCGGCGGGATCATCGACTGGGTCAAGGAGCCTTTGGGTGGCCCCAAC
GTGGCTGATTTGCTTTCGGATAAAGAGGCCCGTCTTCGATGCTCGTGGATGGCGGGCATTCCGGCGCCCATACTGTCCAG
CACGGACAGAATCGAGAATACCGAATGGTTCGCCGGACTCAAACGCAGGAAGACCATTGGAGGCCGTGTTCCCGGTTTCA
AGTCGCGGCATCGCGGCCTTGGTTTCGTCTGCTGGCGCAACGCCTCCAAGACAGGCAACGCGCTTTTTCATCGGACCGGC
CGGAAGTCCGGTGTGGTCGTCATCGCCGGCATGAATCCTACAAGATGGCGCAAGCCCGGTGAAAAGCTCTATTGGCGCAT
CGTCATCCATGTGCGCGTGAGCCAGCCGATACGGCCCTACACCAGCGTCCATGTCGACTGGACCCATCGTAGCCTCGTGT
TCGTCAATATGCCATCGCCATTGCCAAGAATGGGCAACGGCATGGTCGGCATCGACCGTGGCTGCGTGCACACTCTGGCT
TTGTCCGACAGGACCTTCAGGGACATGCCGAAGCCGTCGAAGGCCGAGCTGAAGCGGTTGAAATACCTCCAGCGCCGGAT
GACCCGCCAGGACCGGACCAACGAGGCCAGAGGCGGCAGGATCGCCAAGTTCGAGTCGAAGCGCCGTCGGAAGACCTTGT
CCGAGTTCAACGCCCTGAACAGCCGTATTATTCGTCGCCGCAACGACTGGATCGAGAAGACCACGACAAGGCTCGCCAAG
GCCAACATTCTCATCGCCATGGAGGACCTAGACGTCCAAGCCATGACGAAGCGGCCGAAGCCGAGGCCGGATCCGGCGAA
TCCGGGCCGATACCTCCATAACGGCGCGAAGGCCAAGGCCGGGCTCGACCGGTCGATTTTAATCAACTGTTGGGGTCGCA
TATCGAAGCGGCTTCAGGACAAGATGGGCGCCAATGGCGGCCGTCTTGCCATCGTCCCCGCCGCCTATACCAGCCAAGCC
TGCCATGAGTGCGGGCATGTCGCCAAGGGGAACCGCGAGAGCCAAGCGGTCTTCCATTGCGTCAAGTGCGGGTATAGGGC
GAACGCGGACGTCAACGCGGCTGAAAACATTCTCAGCCGCGCTTTAGCCAAAACGGGCGGGGATACCGCCTTGGACGTGG
AGGATCCGCATTGGCGGCCCGATGAAGCGTCAACCCCCTCAACTCAGGCCGGAAGTTCCGTTGGAGTCCGGGCTGTGAAG
GGAATCCCCCGGCTTTAG

Upstream 100 bases:

>100_bases
AGCCGTGCACGCCATGACCGAAAAAATTCATGCCCCGCGCTTCAGCGCTGGGGTTCAAGGCCGGGACGCATAGGACCGTG
ATGCAAGGAGGTGGTCCACG

Downstream 100 bases:

>100_bases
CCGTGGGGAGGATGTCAACCTGCGTCCAGGAGGATGCAGGCCGGAACATGCGACCCCGCCCCGTATAGCAGGCAGCCGGA
ACAAGATCCGGTATTCGTAG

Product: transposase, IS605 OrfB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MSQKVRIDGVKHVGASVFLGVDHCGNPCWTRNPERVMVWLCDGWRTRFNQRREQRPHNVPVKDEAGGIIDWVKEPLGGPN
VADLLSDKEARLRCSWMAGIPAPILSSTDRIENTEWFAGLKRRKTIGGRVPGFKSRHRGLGFVCWRNASKTGNALFHRTG
RKSGVVVIAGMNPTRWRKPGEKLYWRIVIHVRVSQPIRPYTSVHVDWTHRSLVFVNMPSPLPRMGNGMVGIDRGCVHTLA
LSDRTFRDMPKPSKAELKRLKYLQRRMTRQDRTNEARGGRIAKFESKRRRKTLSEFNALNSRIIRRRNDWIEKTTTRLAK
ANILIAMEDLDVQAMTKRPKPRPDPANPGRYLHNGAKAKAGLDRSILINCWGRISKRLQDKMGANGGRLAIVPAAYTSQA
CHECGHVAKGNRESQAVFHCVKCGYRANADVNAAENILSRALAKTGGDTALDVEDPHWRPDEASTPSTQAGSSVGVRAVK
GIPRL

Sequences:

>Translated_485_residues
MSQKVRIDGVKHVGASVFLGVDHCGNPCWTRNPERVMVWLCDGWRTRFNQRREQRPHNVPVKDEAGGIIDWVKEPLGGPN
VADLLSDKEARLRCSWMAGIPAPILSSTDRIENTEWFAGLKRRKTIGGRVPGFKSRHRGLGFVCWRNASKTGNALFHRTG
RKSGVVVIAGMNPTRWRKPGEKLYWRIVIHVRVSQPIRPYTSVHVDWTHRSLVFVNMPSPLPRMGNGMVGIDRGCVHTLA
LSDRTFRDMPKPSKAELKRLKYLQRRMTRQDRTNEARGGRIAKFESKRRRKTLSEFNALNSRIIRRRNDWIEKTTTRLAK
ANILIAMEDLDVQAMTKRPKPRPDPANPGRYLHNGAKAKAGLDRSILINCWGRISKRLQDKMGANGGRLAIVPAAYTSQA
CHECGHVAKGNRESQAVFHCVKCGYRANADVNAAENILSRALAKTGGDTALDVEDPHWRPDEASTPSTQAGSSVGVRAVK
GIPRL
>Mature_484_residues
SQKVRIDGVKHVGASVFLGVDHCGNPCWTRNPERVMVWLCDGWRTRFNQRREQRPHNVPVKDEAGGIIDWVKEPLGGPNV
ADLLSDKEARLRCSWMAGIPAPILSSTDRIENTEWFAGLKRRKTIGGRVPGFKSRHRGLGFVCWRNASKTGNALFHRTGR
KSGVVVIAGMNPTRWRKPGEKLYWRIVIHVRVSQPIRPYTSVHVDWTHRSLVFVNMPSPLPRMGNGMVGIDRGCVHTLAL
SDRTFRDMPKPSKAELKRLKYLQRRMTRQDRTNEARGGRIAKFESKRRRKTLSEFNALNSRIIRRRNDWIEKTTTRLAKA
NILIAMEDLDVQAMTKRPKPRPDPANPGRYLHNGAKAKAGLDRSILINCWGRISKRLQDKMGANGGRLAIVPAAYTSQAC
HECGHVAKGNRESQAVFHCVKCGYRANADVNAAENILSRALAKTGGDTALDVEDPHWRPDEASTPSTQAGSSVGVRAVKG
IPRL

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG0675

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transposase 35 family [H]

Homologues:

Organism=Escherichia coli, GI288549314, Length=213, Percent_Identity=38.9671361502347, Blast_Score=125, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001959
- InterPro:   IPR010095
- InterPro:   IPR021027 [H]

Pfam domain/function: PF12323 HTH_14; PF01385 Transposase_2; PF07282 Transposase_35 [H]

EC number: NA

Molecular weight: Translated: 54448; Mature: 54317

Theoretical pI: Translated: 11.32; Mature: 11.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKVRIDGVKHVGASVFLGVDHCGNPCWTRNPERVMVWLCDGWRTRFNQRREQRPHNVP
CCCCEEECCHHHCCCEEEEEHHHCCCCCCCCCCCEEEEEEECCHHHHHHHHHHCCCCCCC
VKDEAGGIIDWVKEPLGGPNVADLLSDKEARLRCSWMAGIPAPILSSTDRIENTEWFAGL
CCCCCCCHHHHHHCCCCCCCHHHHHCCCCHHEEEEEECCCCHHHHCCCCCCCCHHHHHHH
KRRKTIGGRVPGFKSRHRGLGFVCWRNASKTGNALFHRTGRKSGVVVIAGMNPTRWRKPG
HHHHHCCCCCCCCCHHCCCCEEEEECCCCCCCCHHHEECCCCCCEEEEECCCCHHCCCCC
EKLYWRIVIHVRVSQPIRPYTSVHVDWTHRSLVFVNMPSPLPRMGNGMVGIDRGCVHTLA
CEEEEEEEEEEEECCCCCCCCEEEEEEECCEEEEEECCCCCHHHCCCCEEECCCCEEEEH
LSDRTFRDMPKPSKAELKRLKYLQRRMTRQDRTNEARGGRIAKFESKRRRKTLSEFNALN
CCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHH
SRIIRRRNDWIEKTTTRLAKANILIAMEDLDVQAMTKRPKPRPDPANPGRYLHNGAKAKA
HHHHHHHHHHHHHHHHHHHHCEEEEEEECCCHHHHHCCCCCCCCCCCCCHHHHCCCHHHC
GLDRSILINCWGRISKRLQDKMGANGGRLAIVPAAYTSQACHECGHVAKGNRESQAVFHC
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHCHHCCCCCHHHHHHHH
VKCGYRANADVNAAENILSRALAKTGGDTALDVEDPHWRPDEASTPSTQAGSSVGVRAVK
HHCCCCCCCCCCHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCHHHHC
GIPRL
CCCCC
>Mature Secondary Structure 
SQKVRIDGVKHVGASVFLGVDHCGNPCWTRNPERVMVWLCDGWRTRFNQRREQRPHNVP
CCCEEECCHHHCCCEEEEEHHHCCCCCCCCCCCEEEEEEECCHHHHHHHHHHCCCCCCC
VKDEAGGIIDWVKEPLGGPNVADLLSDKEARLRCSWMAGIPAPILSSTDRIENTEWFAGL
CCCCCCCHHHHHHCCCCCCCHHHHHCCCCHHEEEEEECCCCHHHHCCCCCCCCHHHHHHH
KRRKTIGGRVPGFKSRHRGLGFVCWRNASKTGNALFHRTGRKSGVVVIAGMNPTRWRKPG
HHHHHCCCCCCCCCHHCCCCEEEEECCCCCCCCHHHEECCCCCCEEEEECCCCHHCCCCC
EKLYWRIVIHVRVSQPIRPYTSVHVDWTHRSLVFVNMPSPLPRMGNGMVGIDRGCVHTLA
CEEEEEEEEEEEECCCCCCCCEEEEEEECCEEEEEECCCCCHHHCCCCEEECCCCEEEEH
LSDRTFRDMPKPSKAELKRLKYLQRRMTRQDRTNEARGGRIAKFESKRRRKTLSEFNALN
CCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHH
SRIIRRRNDWIEKTTTRLAKANILIAMEDLDVQAMTKRPKPRPDPANPGRYLHNGAKAKA
HHHHHHHHHHHHHHHHHHHHCEEEEEEECCCHHHHHCCCCCCCCCCCCCHHHHCCCHHHC
GLDRSILINCWGRISKRLQDKMGANGGRLAIVPAAYTSQACHECGHVAKGNRESQAVFHC
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHCHHCCCCCHHHHHHHH
VKCGYRANADVNAAENILSRALAKTGGDTALDVEDPHWRPDEASTPSTQAGSSVGVRAVK
HHCCCCCCCCCCHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCHHHHC
GIPRL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]