Click here to switch to the map view.

The map label for this gene is hrdB [H]

Identifier: 213691851

GI number: 213691851

Start: 1134482

End: 1135876

Strand: Direct

Name: hrdB [H]

Synonym: Blon_0960

Alternate gene names: 213691851

Gene position: 1134482-1135876 (Clockwise)

Preceding gene: 213691850

Following gene: 213691852

Centisome position: 40.05

GC content: 60.36

Gene sequence:

>1395_bases
TTGGCCACGAAGGAAACGACGGCAACCAAGCAGACAGCCGAGTCCAGCGAGGAGACCGAGAAGAAGGCCAGCTCTACGAC
CACCGCACGCAAGACTTCCGTCAAGAAGTCCAAGTCCGCAGAGCCCTCGGAAAAGACCACGCGCAAGTCCGCCGCGAAGA
AAACCGCGAAGAAGGCCAAGGACGATGAACTCGTCAAAGACGAGGACACTCAGGTCGATGACGGGCTTGATGACGATGCC
GAGGTCAACGACGACGATTTGGACGTCGACGATCTCGACGAGGATCTGGACGACGTTGACGAAGACGATGCCGACGACGA
CGATGTGGAAGAGGATGAGGACGAGGACGAGGAAGAAGCCAAGTCCACGGCACCCGAGCAGCCCAAGGAAAAGGGTGCCT
ACGTGGTCTCCGACACGGATGATGAGGAAGAAAACATCATCCCTTCGGGCAATCCGAAGCGTCGTGTGATTGCGGCCGGC
GCAACCGCCGACCCGGTCAAGGACTACCTGAAGCAGATCGGCCGCGTGAGCCTGCTGAACGCCGAGCAGGAAGTCGACCT
GTCCGAGCGCATCGAGGCCGGCCTGTACGCCCAGCACCTGCTCGACACCGAAAGCGAAGGCATGGAGTTCAAGCGCAAGC
GTGAACTCAAGTGGGCCGCCGCCGACGGCAAGAAGGCTAAGGACCATCTGCTGGAAGCCAACCTACGACTTGTGGTCTCG
CTCGCCAAGCGCTACACGGGTCGCGGCATGCTCTTCCTTGATTTGATCCAGGAAGGCAACCTTGGTCTGATTCGTGCCGT
CGAGAAGTTCGACTGGAAGAAGGGCTTCAAGTTCTCCACCTACGCCACGTGGTGGATTCGTCAGGCCATCACCCGTGCCA
TGGCCGATCAAGCCCGCACCATTCGCGTGCCTGTGCACATGGTTGAAGTGATCAACAAGCTGTCCCGTGTACAGCGCCAG
ATGCTGCAGGATCTGGGGCGTGAACCTACGCCGGACGAGCTGGCCCGCGAGCTGGATATGCCGGTTGAGAAGGTGCAGGA
AGTGCAGAAGTACGGGCGCGAGCCGATTTCCCTGCACACCCCGCTTGGCGAGGATGGCGATTCCGAGTTCGGTGACCTCA
TTGAGGATACCGACGCCATCGCTCCGTCCGACGCCGTGGCCTTCTCCCTGCTGCAGGAACAGTTCAAGCAGGTGCTGGAA
ACCCTGTCTCCGCGTGAGGCCGGCGTCATCAAGATGCGCTATGGCCTGGAAGACGGCCAGCCGAAGACGCTGGACGACAT
CGGCCGTGTGTATGGCGTGACCCGTGAACGAATCCGTCAGATCGAGTCCAAGACCATGTCCAAGCTGCGCCACCCGTCCC
GTTCACAGACCCTGCGCGACTTCCTGGATCAATGA

Upstream 100 bases:

>100_bases
CGTGGAGGGTGGTTTACTCACATAGTTGACGATTTGCCCTTGCTAGAAGTGGGCCCATGTGGTATATGCGCTTCACGGCG
AGAGATCAGAAGGAGCAGTT

Downstream 100 bases:

>100_bases
TCATCTGGCTCCCCTCTGTGAGGGGAGCCAAGCATTTGAAGAGAAAAATAGGACTGTATGCCCAAAGAAGAATACGGCGC
CAAGGACCTAGCTGTTCTTG

Product: RNA polymerase sigma factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MATKETTATKQTAESSEETEKKASSTTTARKTSVKKSKSAEPSEKTTRKSAAKKTAKKAKDDELVKDEDTQVDDGLDDDA
EVNDDDLDVDDLDEDLDDVDEDDADDDDVEEDEDEDEEEAKSTAPEQPKEKGAYVVSDTDDEEENIIPSGNPKRRVIAAG
ATADPVKDYLKQIGRVSLLNAEQEVDLSERIEAGLYAQHLLDTESEGMEFKRKRELKWAAADGKKAKDHLLEANLRLVVS
LAKRYTGRGMLFLDLIQEGNLGLIRAVEKFDWKKGFKFSTYATWWIRQAITRAMADQARTIRVPVHMVEVINKLSRVQRQ
MLQDLGREPTPDELARELDMPVEKVQEVQKYGREPISLHTPLGEDGDSEFGDLIEDTDAIAPSDAVAFSLLQEQFKQVLE
TLSPREAGVIKMRYGLEDGQPKTLDDIGRVYGVTRERIRQIESKTMSKLRHPSRSQTLRDFLDQ

Sequences:

>Translated_464_residues
MATKETTATKQTAESSEETEKKASSTTTARKTSVKKSKSAEPSEKTTRKSAAKKTAKKAKDDELVKDEDTQVDDGLDDDA
EVNDDDLDVDDLDEDLDDVDEDDADDDDVEEDEDEDEEEAKSTAPEQPKEKGAYVVSDTDDEEENIIPSGNPKRRVIAAG
ATADPVKDYLKQIGRVSLLNAEQEVDLSERIEAGLYAQHLLDTESEGMEFKRKRELKWAAADGKKAKDHLLEANLRLVVS
LAKRYTGRGMLFLDLIQEGNLGLIRAVEKFDWKKGFKFSTYATWWIRQAITRAMADQARTIRVPVHMVEVINKLSRVQRQ
MLQDLGREPTPDELARELDMPVEKVQEVQKYGREPISLHTPLGEDGDSEFGDLIEDTDAIAPSDAVAFSLLQEQFKQVLE
TLSPREAGVIKMRYGLEDGQPKTLDDIGRVYGVTRERIRQIESKTMSKLRHPSRSQTLRDFLDQ
>Mature_463_residues
ATKETTATKQTAESSEETEKKASSTTTARKTSVKKSKSAEPSEKTTRKSAAKKTAKKAKDDELVKDEDTQVDDGLDDDAE
VNDDDLDVDDLDEDLDDVDEDDADDDDVEEDEDEDEEEAKSTAPEQPKEKGAYVVSDTDDEEENIIPSGNPKRRVIAAGA
TADPVKDYLKQIGRVSLLNAEQEVDLSERIEAGLYAQHLLDTESEGMEFKRKRELKWAAADGKKAKDHLLEANLRLVVSL
AKRYTGRGMLFLDLIQEGNLGLIRAVEKFDWKKGFKFSTYATWWIRQAITRAMADQARTIRVPVHMVEVINKLSRVQRQM
LQDLGREPTPDELARELDMPVEKVQEVQKYGREPISLHTPLGEDGDSEFGDLIEDTDAIAPSDAVAFSLLQEQFKQVLET
LSPREAGVIKMRYGLEDGQPKTLDDIGRVYGVTRERIRQIESKTMSKLRHPSRSQTLRDFLDQ

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=283, Percent_Identity=53.7102473498233, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI1789098, Length=363, Percent_Identity=36.6391184573003, Blast_Score=212, Evalue=5e-56,
Organism=Escherichia coli, GI1789871, Length=243, Percent_Identity=28.8065843621399, Blast_Score=83, Evalue=4e-17,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 52237; Mature: 52106

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATKETTATKQTAESSEETEKKASSTTTARKTSVKKSKSAEPSEKTTRKSAAKKTAKKAK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
DDELVKDEDTQVDDGLDDDAEVNDDDLDVDDLDEDLDDVDEDDADDDDVEEDEDEDEEEA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
KSTAPEQPKEKGAYVVSDTDDEEENIIPSGNPKRRVIAAGATADPVKDYLKQIGRVSLLN
HHCCCCCHHHCCCEEEECCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCEECCC
AEQEVDLSERIEAGLYAQHLLDTESEGMEFKRKRELKWAAADGKKAKDHLLEANLRLVVS
CHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LAKRYTGRGMLFLDLIQEGNLGLIRAVEKFDWKKGFKFSTYATWWIRQAITRAMADQART
HHHHHCCCCEEEEEHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCE
IRVPVHMVEVINKLSRVQRQMLQDLGREPTPDELARELDMPVEKVQEVQKYGREPISLHT
EECHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCEEEEC
PLGEDGDSEFGDLIEDTDAIAPSDAVAFSLLQEQFKQVLETLSPREAGVIKMRYGLEDGQ
CCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCC
PKTLDDIGRVYGVTRERIRQIESKTMSKLRHPSRSQTLRDFLDQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure 
ATKETTATKQTAESSEETEKKASSTTTARKTSVKKSKSAEPSEKTTRKSAAKKTAKKAK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
DDELVKDEDTQVDDGLDDDAEVNDDDLDVDDLDEDLDDVDEDDADDDDVEEDEDEDEEEA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
KSTAPEQPKEKGAYVVSDTDDEEENIIPSGNPKRRVIAAGATADPVKDYLKQIGRVSLLN
HHCCCCCHHHCCCEEEECCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCEECCC
AEQEVDLSERIEAGLYAQHLLDTESEGMEFKRKRELKWAAADGKKAKDHLLEANLRLVVS
CHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LAKRYTGRGMLFLDLIQEGNLGLIRAVEKFDWKKGFKFSTYATWWIRQAITRAMADQART
HHHHHCCCCEEEEEHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCE
IRVPVHMVEVINKLSRVQRQMLQDLGREPTPDELARELDMPVEKVQEVQKYGREPISLHT
EECHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCEEEEC
PLGEDGDSEFGDLIEDTDAIAPSDAVAFSLLQEQFKQVLETLSPREAGVIKMRYGLEDGQ
CCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCC
PKTLDDIGRVYGVTRERIRQIESKTMSKLRHPSRSQTLRDFLDQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8690707 [H]