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The map label for this gene is ispH [H]

Identifier: 213691790

GI number: 213691790

Start: 1063634

End: 1064626

Strand: Direct

Name: ispH [H]

Synonym: Blon_0898

Alternate gene names: 213691790

Gene position: 1063634-1064626 (Clockwise)

Preceding gene: 213691787

Following gene: 213691794

Centisome position: 37.55

GC content: 59.32

Gene sequence:

>993_bases
ATGACCAAAAGCGTTGTGCTCGCTGATCCGCGAGGCTTCTGCGCGGGTGTGGACCGTGCGATTCTTACCGTACAGACCAT
TCTCAAAGCCGCGGAGGCTTCCGGAAAGCGGACGCGCGAGGATGGGCTGCCCCCGGTATATGTGCGCCGGCAGATCGTGC
ACAACAAGCATGTGGTCGAGGACTTGGCCGGGCAAGGCGCGGTGTTCGTCCAGGAATTGGCGGAGATTCCGGATGCTGCT
GCCCAAGCGGGTATTCCGGTGGTGTTTTCCGCCCATGGCGTATCTCCGGTAGTGAAGGCGGAAGCCGAACGTCGTGGCAT
GCATGTGGTGGATGCCACCTGCCCTCTGGTGGGTAAGGTGCACCGTGAGGTGCTGCGTTTCGTGCGCAAGGGGTATGAAA
TCGTCTATATCGGGCACAAGGGCCACGATGAAGCAGTCGGCGTGGTGGGGGAGAACCCCGAACATGTGCACTTGATCGAG
CATGAATCGGATGTCGATTCGTTGGATTTTGCGCCCGACACCAAGTTGGTGCTGCTCTCGCAGACCACGTTGAGCGTGGA
TGAGACCGCCGGCACCATTGCCGCGCTGAAGGCCAAGTTCCCGTGGATTCAAGAGCCGCCGAGTTCGGATATCTGCTATG
CCACGTCGAATCGTCAGGCTGCGGTCAAACTGGTGGCCCAGCAGTCCGATTGCGTGGTGATTGTCGGATCGGCCAACTCC
TCGAATTCCGTGCGACTGATGGAGGTGGCGCAGGAGGGCTTGGGGAAGCGCGGCAAAGCGTATCGCGTGGATGATGCCAG
CGAGCTTGATCCGGCGTGGTTCGTCGGACTGGAGTCCGTGGGTATCTCCTCAGGTGCCTCCGTGCCGGACGAACTGGTGT
CCGGTGTGATTGACGCTCTGCAAAACTTGGGTTTCACCGGTATGAAGTCCATCGAAACCATCAAAGAAAACATGCACTTC
GTGTTGCCGGCGGAGTTGCGTAGGAAGAAATAG

Upstream 100 bases:

>100_bases
GAAACCGGTGATTCGTGGGAGACACGGAATAGTTGTGACGGCAGATTCCTGTAATCCCTGCCTTACACTGGAAAACACTA
TTGACGTTCAGGAGGTTCCG

Downstream 100 bases:

>100_bases
TCATCCTTGGCTCCCCTCACCGAGGGAAGCCAAGAACGGTCACCACGTGCCGATTGGTGCGGCAATGGCGTTCATCACCT
TCAGCAGGTCCTGCGGGTTC

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MTKSVVLADPRGFCAGVDRAILTVQTILKAAEASGKRTREDGLPPVYVRRQIVHNKHVVEDLAGQGAVFVQELAEIPDAA
AQAGIPVVFSAHGVSPVVKAEAERRGMHVVDATCPLVGKVHREVLRFVRKGYEIVYIGHKGHDEAVGVVGENPEHVHLIE
HESDVDSLDFAPDTKLVLLSQTTLSVDETAGTIAALKAKFPWIQEPPSSDICYATSNRQAAVKLVAQQSDCVVIVGSANS
SNSVRLMEVAQEGLGKRGKAYRVDDASELDPAWFVGLESVGISSGASVPDELVSGVIDALQNLGFTGMKSIETIKENMHF
VLPAELRRKK

Sequences:

>Translated_330_residues
MTKSVVLADPRGFCAGVDRAILTVQTILKAAEASGKRTREDGLPPVYVRRQIVHNKHVVEDLAGQGAVFVQELAEIPDAA
AQAGIPVVFSAHGVSPVVKAEAERRGMHVVDATCPLVGKVHREVLRFVRKGYEIVYIGHKGHDEAVGVVGENPEHVHLIE
HESDVDSLDFAPDTKLVLLSQTTLSVDETAGTIAALKAKFPWIQEPPSSDICYATSNRQAAVKLVAQQSDCVVIVGSANS
SNSVRLMEVAQEGLGKRGKAYRVDDASELDPAWFVGLESVGISSGASVPDELVSGVIDALQNLGFTGMKSIETIKENMHF
VLPAELRRKK
>Mature_329_residues
TKSVVLADPRGFCAGVDRAILTVQTILKAAEASGKRTREDGLPPVYVRRQIVHNKHVVEDLAGQGAVFVQELAEIPDAAA
QAGIPVVFSAHGVSPVVKAEAERRGMHVVDATCPLVGKVHREVLRFVRKGYEIVYIGHKGHDEAVGVVGENPEHVHLIEH
ESDVDSLDFAPDTKLVLLSQTTLSVDETAGTIAALKAKFPWIQEPPSSDICYATSNRQAAVKLVAQQSDCVVIVGSANSS
NSVRLMEVAQEGLGKRGKAYRVDDASELDPAWFVGLESVGISSGASVPDELVSGVIDALQNLGFTGMKSIETIKENMHFV
LPAELRRKK

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=326, Percent_Identity=46.6257668711656, Blast_Score=278, Evalue=5e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 35439; Mature: 35308

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSVVLADPRGFCAGVDRAILTVQTILKAAEASGKRTREDGLPPVYVRRQIVHNKHVVE
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHHHHH
DLAGQGAVFVQELAEIPDAAAQAGIPVVFSAHGVSPVVKAEAERRGMHVVDATCPLVGKV
HHCCCCHHHHHHHHHCCCHHHHCCCCEEEECCCCCHHHHHHHHHCCCEEEECCCHHHHHH
HREVLRFVRKGYEIVYIGHKGHDEAVGVVGENPEHVHLIEHESDVDSLDFAPDTKLVLLS
HHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCEEEEEE
QTTLSVDETAGTIAALKAKFPWIQEPPSSDICYATSNRQAAVKLVAQQSDCVVIVGSANS
CCEECCHHHCCHHHHHHHCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCEEEEEECCCC
SNSVRLMEVAQEGLGKRGKAYRVDDASELDPAWFVGLESVGISSGASVPDELVSGVIDAL
CCCEEHHHHHHHCCCCCCCEEECCCCCCCCCHHEEEHHHCCCCCCCCCHHHHHHHHHHHH
QNLGFTGMKSIETIKENMHFVLPAELRRKK
HHCCCCCHHHHHHHHHCCEEEECHHHHCCC
>Mature Secondary Structure 
TKSVVLADPRGFCAGVDRAILTVQTILKAAEASGKRTREDGLPPVYVRRQIVHNKHVVE
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHHHHH
DLAGQGAVFVQELAEIPDAAAQAGIPVVFSAHGVSPVVKAEAERRGMHVVDATCPLVGKV
HHCCCCHHHHHHHHHCCCHHHHCCCCEEEECCCCCHHHHHHHHHCCCEEEECCCHHHHHH
HREVLRFVRKGYEIVYIGHKGHDEAVGVVGENPEHVHLIEHESDVDSLDFAPDTKLVLLS
HHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCEEEEEE
QTTLSVDETAGTIAALKAKFPWIQEPPSSDICYATSNRQAAVKLVAQQSDCVVIVGSANS
CCEECCHHHCCHHHHHHHCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCEEEEEECCCC
SNSVRLMEVAQEGLGKRGKAYRVDDASELDPAWFVGLESVGISSGASVPDELVSGVIDAL
CCCEEHHHHHHHCCCCCCCEEECCCCCCCCCHHEEEHHHCCCCCCCCCHHHHHHHHHHHH
QNLGFTGMKSIETIKENMHFVLPAELRRKK
HHCCCCCHHHHHHHHHCCEEEECHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12381787 [H]