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The map label for this gene is dnaE [H]

Identifier: 213691703

GI number: 213691703

Start: 936943

End: 939579

Strand: Direct

Name: dnaE [H]

Synonym: Blon_0808

Alternate gene names: 213691703

Gene position: 936943-939579 (Clockwise)

Preceding gene: 213691701

Following gene: 213691704

Centisome position: 33.08

GC content: 47.36

Gene sequence:

>2637_bases
ATGCAGCCGATTCGAATTCGAAGCAACCATGACTATGGAGGCGGTGCTCTTATGCGGGATTTCGTTAATTTGCATGTACA
TAGTGACTATTCGCTGGCAGATGGTGTCTCTACTGTGAGCGAGCTGATGAGTGAAGCGCAACGATTAGGTCAGCCAGCAA
TTGGTCTGACTGATCACGGCAGTGTCGGCGGTTTGATGGAGTTCTGGAAAGCAGGACGTCAGCGAGGCATTAAGCCGATT
TTGGGTGTTGAAGCATACGTAACGCCAGAAACTGGCCGCACGGATACGAGATGCGTATCTTGGGATAATTCGTGGAAAGA
GAGGAATACTCGAAGAAGTGTTACTTGTGTGCCTCATGATCCTTATGATGTCAGCGGTGGTGGGCTGTTTACTCACTTGA
CGCTATGGGCAGAGTCCGATGAAGGTCTTGTCAATCTCATGAAAGCATCTTCCGTGGCGAATCTCGAAGGTCTGGTCTCG
CGTTATCCTCGCATGGATAACAATGTCTTGTCATCATTTGCAAAAGGACTTATTTGTGGGTCGGGCTGCATGTCTGGAGC
CATTCCGACAAGATTGTTGCTTGGACAATTTGATGAAGCGTTGCGAATGGCCGGTGAACTACAGGATATTTTCGGTCGAG
ATAATTTCTTTATAGAAATCATGGATCATGGTTTGTCCATTGAGCACCAAATCAGGAATGATCTAGTGGAACTGGCGAAA
CGAATTAATGCGCCGTTAGTCGCAACTAGTGATGTGCATTATGCAAAGCGTGATGACAGTCTAAAACATGATGTGCGTAT
ATGCATTGAAACAGGTGATGTGATGGATGGTCCTCATCGTTTCAAGTTTGAAAGCGAGGAGTATTATTTGCGTTCATCTG
CGGTTATGCGGTCTCTGTTCTCCGATGTGCCTGAAGCCTGCGACAATACGTTGCTCATAGCAGAGCGGTGCGATACCGGA
TTTTGTGTAGCGGAAGATGGCTCATTAATGCCGCAGCATGTTGGTGCTGATGGCCGAGCGTCGAGTGAAATTCTTCAAGG
GGAAGTTGAAGGATGGCTGCAAACGAAGTATGGAAGCATACCGGATTCAGTGTGGAATCGTGCTCAACACGAAATGGCTG
TGATTCGTGAAACCCATGCTGAAGAATACTTGATGGCCGTCGCATATTGTACGAATCGATTGCGAGAAGAGGGGCATTTG
GTCGGGCCCGGGAAGGGGATATTGGCTCGCAGCCTTGTCGCCTATGCGCTGGGGATTAGTGAGATTGATCCCATCGAATA
CGATTTACCCTTTACTGATTTTCAAGATTCAGTCTTTCCTACAGCTGTTTTGCAAACGGAACGGGGACTGTCCAGGCGGG
TATTGCAATATCTATGTGACCGATATGGGAATGGTTATGCAGCTGCTGTAATTGCATACAGCCGTTTTAACGATGCCAAG
GCAGTGAAAGATGTGTCGCATGTATGCGGATATCAACGGTTCTGGGATGCATCCTCGGCTGAGGTTTGGTTGCAGGATAA
CGCCAAGAAGTCTATTGCGGATGGGTTGAAAGACTGTATATATGACTGTTCCGTTCGCAGTGATGTGTTGGCTCTTTGCA
ACAAGCCCCTTGCCGAAGTAACGTCCATTGTTCGTTTTCCTTCGGGGGCAGAAAGCACCGCGTTTGATGCAAAAGGCTGT
GATGAACTTGGGCTTCCTCGTCTCCGTATTGCAGGTTCGAGAGTTTTGCAGGTGGTGCAGGATGCCCTTCGATTCATACC
GGATTGCAATGTGCCGCAAGTATGGGACGATTCTTGCAGTTCGTTGGATGAGAGCCGTGTGTATGACTTACTGTCCGCGG
GCTATACGGCCGGTATTCCATTCTTGGATGGAATGAAGAGCGAACTGATGCAGCAGAAGCTTACCTCTTTTGCGGAGCTA
ATTGACATATGCCATAGCAGAGGTGCCGACCGCGCAGATGCTGTTGCTCGTGCAATGTTCGCATACCGTGTCGCCTATGT
GAAAACGTTATACACAATCGAATTCATGACCGCAATGTTGAATAGTTATAACGAGGATGGCGCTAGAGCGAAACTGGAAA
GTATATTTGAAGAAATGTACTGTTTAGGTATTCATGTAAGAATGCCAAACATTAATCAATCTCAATATTATGCGACAGCG
TATTCCAGTACCGGTGACGATGAGAAATCGGCATATGGAATTCAGCTCGGTTTTAATGCGATACGGGGCATTTCGGAACA
ATTGGCAGAAGAAATCGTTTCAACCCGTAATAAGGGCAGTGCCTATCGCAGTTTTAATGATTTTATACGTCGTGTTTCAT
TGGAACATTGCAATGCAGGGAATGTCAATTACCTCATTAGAGCCGGTGCTTTTGATTGCGTCAACCCCTGCAGGAAGGCT
CTTGCCCAATGTGGCAATGACCTTATCGAGGCGGAAAGGCAATACAGAGCAGCACAATCCCATGGACAGGGTGATTTATT
GCAGGATGTAATGGATGCAAGTCCTTGTGCGCAAAAGAGTACAGATGAAGTCGTCCCTAATGGTTCGGATTGGGATCAAA
CCACTCGGCAACAGCTCCAGTGTGAGTCTTTCAAATGGCCAATAGGACGTGCGATTGAACAATATGTGTGTGCTTAG

Upstream 100 bases:

>100_bases
TGCGATGAGACGGCCATCATGTTCCTCTCGCTGAGTATCGAGACTGCTTCAAGAACCAGTAGATAATTACCGTTGATTGT
AGCGCGGCAGGAAACTGAAC

Downstream 100 bases:

>100_bases
ACGGCAATGTGATACTACGAAATCACTAGGCTTGCGTAAGAGTGCAAAGTGTCATGCGCGAAGTCCCGGATATTTGTTGC
GTCAGTTATATGCTGAGAAC

Product: DNA polymerase III, alpha subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 878; Mature: 878

Protein sequence:

>878_residues
MQPIRIRSNHDYGGGALMRDFVNLHVHSDYSLADGVSTVSELMSEAQRLGQPAIGLTDHGSVGGLMEFWKAGRQRGIKPI
LGVEAYVTPETGRTDTRCVSWDNSWKERNTRRSVTCVPHDPYDVSGGGLFTHLTLWAESDEGLVNLMKASSVANLEGLVS
RYPRMDNNVLSSFAKGLICGSGCMSGAIPTRLLLGQFDEALRMAGELQDIFGRDNFFIEIMDHGLSIEHQIRNDLVELAK
RINAPLVATSDVHYAKRDDSLKHDVRICIETGDVMDGPHRFKFESEEYYLRSSAVMRSLFSDVPEACDNTLLIAERCDTG
FCVAEDGSLMPQHVGADGRASSEILQGEVEGWLQTKYGSIPDSVWNRAQHEMAVIRETHAEEYLMAVAYCTNRLREEGHL
VGPGKGILARSLVAYALGISEIDPIEYDLPFTDFQDSVFPTAVLQTERGLSRRVLQYLCDRYGNGYAAAVIAYSRFNDAK
AVKDVSHVCGYQRFWDASSAEVWLQDNAKKSIADGLKDCIYDCSVRSDVLALCNKPLAEVTSIVRFPSGAESTAFDAKGC
DELGLPRLRIAGSRVLQVVQDALRFIPDCNVPQVWDDSCSSLDESRVYDLLSAGYTAGIPFLDGMKSELMQQKLTSFAEL
IDICHSRGADRADAVARAMFAYRVAYVKTLYTIEFMTAMLNSYNEDGARAKLESIFEEMYCLGIHVRMPNINQSQYYATA
YSSTGDDEKSAYGIQLGFNAIRGISEQLAEEIVSTRNKGSAYRSFNDFIRRVSLEHCNAGNVNYLIRAGAFDCVNPCRKA
LAQCGNDLIEAERQYRAAQSHGQGDLLQDVMDASPCAQKSTDEVVPNGSDWDQTTRQQLQCESFKWPIGRAIEQYVCA

Sequences:

>Translated_878_residues
MQPIRIRSNHDYGGGALMRDFVNLHVHSDYSLADGVSTVSELMSEAQRLGQPAIGLTDHGSVGGLMEFWKAGRQRGIKPI
LGVEAYVTPETGRTDTRCVSWDNSWKERNTRRSVTCVPHDPYDVSGGGLFTHLTLWAESDEGLVNLMKASSVANLEGLVS
RYPRMDNNVLSSFAKGLICGSGCMSGAIPTRLLLGQFDEALRMAGELQDIFGRDNFFIEIMDHGLSIEHQIRNDLVELAK
RINAPLVATSDVHYAKRDDSLKHDVRICIETGDVMDGPHRFKFESEEYYLRSSAVMRSLFSDVPEACDNTLLIAERCDTG
FCVAEDGSLMPQHVGADGRASSEILQGEVEGWLQTKYGSIPDSVWNRAQHEMAVIRETHAEEYLMAVAYCTNRLREEGHL
VGPGKGILARSLVAYALGISEIDPIEYDLPFTDFQDSVFPTAVLQTERGLSRRVLQYLCDRYGNGYAAAVIAYSRFNDAK
AVKDVSHVCGYQRFWDASSAEVWLQDNAKKSIADGLKDCIYDCSVRSDVLALCNKPLAEVTSIVRFPSGAESTAFDAKGC
DELGLPRLRIAGSRVLQVVQDALRFIPDCNVPQVWDDSCSSLDESRVYDLLSAGYTAGIPFLDGMKSELMQQKLTSFAEL
IDICHSRGADRADAVARAMFAYRVAYVKTLYTIEFMTAMLNSYNEDGARAKLESIFEEMYCLGIHVRMPNINQSQYYATA
YSSTGDDEKSAYGIQLGFNAIRGISEQLAEEIVSTRNKGSAYRSFNDFIRRVSLEHCNAGNVNYLIRAGAFDCVNPCRKA
LAQCGNDLIEAERQYRAAQSHGQGDLLQDVMDASPCAQKSTDEVVPNGSDWDQTTRQQLQCESFKWPIGRAIEQYVCA
>Mature_878_residues
MQPIRIRSNHDYGGGALMRDFVNLHVHSDYSLADGVSTVSELMSEAQRLGQPAIGLTDHGSVGGLMEFWKAGRQRGIKPI
LGVEAYVTPETGRTDTRCVSWDNSWKERNTRRSVTCVPHDPYDVSGGGLFTHLTLWAESDEGLVNLMKASSVANLEGLVS
RYPRMDNNVLSSFAKGLICGSGCMSGAIPTRLLLGQFDEALRMAGELQDIFGRDNFFIEIMDHGLSIEHQIRNDLVELAK
RINAPLVATSDVHYAKRDDSLKHDVRICIETGDVMDGPHRFKFESEEYYLRSSAVMRSLFSDVPEACDNTLLIAERCDTG
FCVAEDGSLMPQHVGADGRASSEILQGEVEGWLQTKYGSIPDSVWNRAQHEMAVIRETHAEEYLMAVAYCTNRLREEGHL
VGPGKGILARSLVAYALGISEIDPIEYDLPFTDFQDSVFPTAVLQTERGLSRRVLQYLCDRYGNGYAAAVIAYSRFNDAK
AVKDVSHVCGYQRFWDASSAEVWLQDNAKKSIADGLKDCIYDCSVRSDVLALCNKPLAEVTSIVRFPSGAESTAFDAKGC
DELGLPRLRIAGSRVLQVVQDALRFIPDCNVPQVWDDSCSSLDESRVYDLLSAGYTAGIPFLDGMKSELMQQKLTSFAEL
IDICHSRGADRADAVARAMFAYRVAYVKTLYTIEFMTAMLNSYNEDGARAKLESIFEEMYCLGIHVRMPNINQSQYYATA
YSSTGDDEKSAYGIQLGFNAIRGISEQLAEEIVSTRNKGSAYRSFNDFIRRVSLEHCNAGNVNYLIRAGAFDCVNPCRKA
LAQCGNDLIEAERQYRAAQSHGQGDLLQDVMDASPCAQKSTDEVVPNGSDWDQTTRQQLQCESFKWPIGRAIEQYVCA

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase [H]

COG id: COG0587

COG function: function code L; DNA polymerase III, alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA polymerase type-C family. DnaE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786381, Length=673, Percent_Identity=27.0430906389302, Blast_Score=197, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011708
- InterPro:   IPR004365
- InterPro:   IPR004013
- InterPro:   IPR003141
- InterPro:   IPR016195
- InterPro:   IPR004805 [H]

Pfam domain/function: PF07733 DNA_pol3_alpha; PF02811 PHP; PF01336 tRNA_anti [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 97395; Mature: 97395

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPIRIRSNHDYGGGALMRDFVNLHVHSDYSLADGVSTVSELMSEAQRLGQPAIGLTDHG
CCCCEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
SVGGLMEFWKAGRQRGIKPILGVEAYVTPETGRTDTRCVSWDNSWKERNTRRSVTCVPHD
CCCHHHHHHHHHHHCCCCCEECCEEEECCCCCCCCCEEECCCCCHHHCCCCCCEEECCCC
PYDVSGGGLFTHLTLWAESDEGLVNLMKASSVANLEGLVSRYPRMDNNVLSSFAKGLICG
CCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEC
SGCMSGAIPTRLLLGQFDEALRMAGELQDIFGRDNFFIEIMDHGLSIEHQIRNDLVELAK
CCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHH
RINAPLVATSDVHYAKRDDSLKHDVRICIETGDVMDGPHRFKFESEEYYLRSSAVMRSLF
HCCCCEEEECCCCHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
SDVPEACDNTLLIAERCDTGFCVAEDGSLMPQHVGADGRASSEILQGEVEGWLQTKYGSI
HHHHHHHCCCEEEEECCCCCEEEECCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCC
PDSVWNRAQHEMAVIRETHAEEYLMAVAYCTNRLREEGHLVGPGKGILARSLVAYALGIS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHCCC
EIDPIEYDLPFTDFQDSVFPTAVLQTERGLSRRVLQYLCDRYGNGYAAAVIAYSRFNDAK
CCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEHEEHHHCCHHH
AVKDVSHVCGYQRFWDASSAEVWLQDNAKKSIADGLKDCIYDCSVRSDVLALCNKPLAEV
HHHHHHHHHCHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHH
TSIVRFPSGAESTAFDAKGCDELGLPRLRIAGSRVLQVVQDALRFIPDCNVPQVWDDSCS
HHHHHCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
SLDESRVYDLLSAGYTAGIPFLDGMKSELMQQKLTSFAELIDICHSRGADRADAVARAMF
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AYRVAYVKTLYTIEFMTAMLNSYNEDGARAKLESIFEEMYCLGIHVRMPNINQSQYYATA
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCEEEEE
YSSTGDDEKSAYGIQLGFNAIRGISEQLAEEIVSTRNKGSAYRSFNDFIRRVSLEHCNAG
ECCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCC
NVNYLIRAGAFDCVNPCRKALAQCGNDLIEAERQYRAAQSHGQGDLLQDVMDASPCAQKS
CCEEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCC
TDEVVPNGSDWDQTTRQQLQCESFKWPIGRAIEQYVCA
CCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MQPIRIRSNHDYGGGALMRDFVNLHVHSDYSLADGVSTVSELMSEAQRLGQPAIGLTDHG
CCCCEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
SVGGLMEFWKAGRQRGIKPILGVEAYVTPETGRTDTRCVSWDNSWKERNTRRSVTCVPHD
CCCHHHHHHHHHHHCCCCCEECCEEEECCCCCCCCCEEECCCCCHHHCCCCCCEEECCCC
PYDVSGGGLFTHLTLWAESDEGLVNLMKASSVANLEGLVSRYPRMDNNVLSSFAKGLICG
CCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEC
SGCMSGAIPTRLLLGQFDEALRMAGELQDIFGRDNFFIEIMDHGLSIEHQIRNDLVELAK
CCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHH
RINAPLVATSDVHYAKRDDSLKHDVRICIETGDVMDGPHRFKFESEEYYLRSSAVMRSLF
HCCCCEEEECCCCHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
SDVPEACDNTLLIAERCDTGFCVAEDGSLMPQHVGADGRASSEILQGEVEGWLQTKYGSI
HHHHHHHCCCEEEEECCCCCEEEECCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCC
PDSVWNRAQHEMAVIRETHAEEYLMAVAYCTNRLREEGHLVGPGKGILARSLVAYALGIS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHCCC
EIDPIEYDLPFTDFQDSVFPTAVLQTERGLSRRVLQYLCDRYGNGYAAAVIAYSRFNDAK
CCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEHEEHHHCCHHH
AVKDVSHVCGYQRFWDASSAEVWLQDNAKKSIADGLKDCIYDCSVRSDVLALCNKPLAEV
HHHHHHHHHCHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHH
TSIVRFPSGAESTAFDAKGCDELGLPRLRIAGSRVLQVVQDALRFIPDCNVPQVWDDSCS
HHHHHCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
SLDESRVYDLLSAGYTAGIPFLDGMKSELMQQKLTSFAELIDICHSRGADRADAVARAMF
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AYRVAYVKTLYTIEFMTAMLNSYNEDGARAKLESIFEEMYCLGIHVRMPNINQSQYYATA
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCEEEEE
YSSTGDDEKSAYGIQLGFNAIRGISEQLAEEIVSTRNKGSAYRSFNDFIRRVSLEHCNAG
ECCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCC
NVNYLIRAGAFDCVNPCRKALAQCGNDLIEAERQYRAAQSHGQGDLLQDVMDASPCAQKS
CCEEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCC
TDEVVPNGSDWDQTTRQQLQCESFKWPIGRAIEQYVCA
CCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]