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The map label for this gene is insF1 [H]

Identifier: 213691570

GI number: 213691570

Start: 776152

End: 776832

Strand: Direct

Name: insF1 [H]

Synonym: Blon_0672

Alternate gene names: 213691570

Gene position: 776152-776832 (Clockwise)

Preceding gene: 213691569

Following gene: 213691571

Centisome position: 27.4

GC content: 64.32

Gene sequence:

>681_bases
ATGAGCGTTACGGCGCCAGGAAGATCAAGGCCGCGCCTGGAGCGGAGGGGCGTCATCACCTCCAGAAGGCGCATCGTCGA
CATCATGAAACGGCGGGGCATGACGAGCGCGTACGCGCGCAGAACGTTCAAACCACACAGGACGCGGGTTAACGAGGCCA
GGCTCGCGAACATCATCGACCGCGAGTTCGACGGCTACGAGCCGCGCACCCGCCTGGCCAGTGACCTGACGTACGTGCGC
GTCGGCGGCAAGTGGGCGTACGCGTGCCTGCTGTCCGGCCTGGCGAACAGGAGCATCGCCGGCCACAGCGCCGACACGGG
CCGCACCGCCGACCTGGTCATGGCCGCGTTCGCCACGCTCGGCTTCCCCCTGACCGAGGTGGAGATGTTCCACACGGACC
GGGGCGGCGGGTTCGACAACGCGAAAATCGTCGGGCTGCTCGATGTGTTCGACATCGGAGGATCGCCGTCGCGCAGGGGC
GACCCGTACGACAACGCCGTGGTCGAATCCACCAACAGGCTCCTGAAGAAGGAGCTCATATACCGGAACCACTACACCAG
CCTGGAACAACTACGCTCCGACCTCAACGACTATGTGTGGTGGTCCGACGACCAAAGACTCCACTCCACCCTCGGATACC
GGAGTCCGAAGGAATTCACCGAACAGGGACTCGTCCTCTAA

Upstream 100 bases:

>100_bases
AGCGTTCCCCGCTCCACCTACTACTGGATGACCGAACATCCCGAAGCCGAGCGGGTGGATCCGATCGCCGGCGACGTGCA
CGCGGTCCGGTGCGACAGCC

Downstream 100 bases:

>100_bases
GAACCTGTCCAACACACCGTTGCCAATCCAGAATGCCTGTGCCACGGGCTGCTTTCGGTGTTCGGACATGTCGGCGGGAT
GCCGCCCGTGCTCGTGCTCG

Product: Integrase, catalytic region

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MSVTAPGRSRPRLERRGVITSRRRIVDIMKRRGMTSAYARRTFKPHRTRVNEARLANIIDREFDGYEPRTRLASDLTYVR
VGGKWAYACLLSGLANRSIAGHSADTGRTADLVMAAFATLGFPLTEVEMFHTDRGGGFDNAKIVGLLDVFDIGGSPSRRG
DPYDNAVVESTNRLLKKELIYRNHYTSLEQLRSDLNDYVWWSDDQRLHSTLGYRSPKEFTEQGLVL

Sequences:

>Translated_226_residues
MSVTAPGRSRPRLERRGVITSRRRIVDIMKRRGMTSAYARRTFKPHRTRVNEARLANIIDREFDGYEPRTRLASDLTYVR
VGGKWAYACLLSGLANRSIAGHSADTGRTADLVMAAFATLGFPLTEVEMFHTDRGGGFDNAKIVGLLDVFDIGGSPSRRG
DPYDNAVVESTNRLLKKELIYRNHYTSLEQLRSDLNDYVWWSDDQRLHSTLGYRSPKEFTEQGLVL
>Mature_225_residues
SVTAPGRSRPRLERRGVITSRRRIVDIMKRRGMTSAYARRTFKPHRTRVNEARLANIIDREFDGYEPRTRLASDLTYVRV
GGKWAYACLLSGLANRSIAGHSADTGRTADLVMAAFATLGFPLTEVEMFHTDRGGGFDNAKIVGLLDVFDIGGSPSRRGD
PYDNAVVESTNRLLKKELIYRNHYTSLEQLRSDLNDYVWWSDDQRLHSTLGYRSPKEFTEQGLVL

Specific function: Involved in the transposition of the insertion sequence IS3 [H]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=190, Percent_Identity=24.7368421052632, Blast_Score=79, Evalue=3e-15,
Organism=Escherichia coli, GI1788405, Length=201, Percent_Identity=30.8457711442786, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787263, Length=201, Percent_Identity=30.8457711442786, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786753, Length=201, Percent_Identity=30.8457711442786, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786570, Length=201, Percent_Identity=30.8457711442786, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786490, Length=201, Percent_Identity=30.8457711442786, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789981, Length=214, Percent_Identity=23.8317757009346, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 25629; Mature: 25498

Theoretical pI: Translated: 10.26; Mature: 10.26

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVTAPGRSRPRLERRGVITSRRRIVDIMKRRGMTSAYARRTFKPHRTRVNEARLANIID
CCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHH
REFDGYEPRTRLASDLTYVRVGGKWAYACLLSGLANRSIAGHSADTGRTADLVMAAFATL
HHCCCCCHHHHHHHCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
GFPLTEVEMFHTDRGGGFDNAKIVGLLDVFDIGGSPSRRGDPYDNAVVESTNRLLKKELI
CCCHHHHHEEECCCCCCCCCCEEEEEEHHEECCCCCCCCCCCCCHHHHHHHHHHHHHHHH
YRNHYTSLEQLRSDLNDYVWWSDDQRLHSTLGYRSPKEFTEQGLVL
HHHHHHHHHHHHHHHHHCEECCCCHHHHHHHCCCCCHHHHHCCCCC
>Mature Secondary Structure 
SVTAPGRSRPRLERRGVITSRRRIVDIMKRRGMTSAYARRTFKPHRTRVNEARLANIID
CCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHH
REFDGYEPRTRLASDLTYVRVGGKWAYACLLSGLANRSIAGHSADTGRTADLVMAAFATL
HHCCCCCHHHHHHHCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
GFPLTEVEMFHTDRGGGFDNAKIVGLLDVFDIGGSPSRRGDPYDNAVVESTNRLLKKELI
CCCHHHHHEEECCCCCCCCCCEEEEEEHHEECCCCCCCCCCCCCHHHHHHHHHHHHHHHH
YRNHYTSLEQLRSDLNDYVWWSDDQRLHSTLGYRSPKEFTEQGLVL
HHHHHHHHHHHHHHHHHCEECCCCHHHHHHHCCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987844; 9278503 [H]