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The map label for this gene is proS [H]

Identifier: 213691491

GI number: 213691491

Start: 665156

End: 666970

Strand: Direct

Name: proS [H]

Synonym: Blon_0589

Alternate gene names: 213691491

Gene position: 665156-666970 (Clockwise)

Preceding gene: 213691489

Following gene: 213691492

Centisome position: 23.48

GC content: 62.48

Gene sequence:

>1815_bases
ATGACTTCCAATACCCTTCGTATGTCAACCATGTTCCTGCGCACCCTGCGCGAGGACCCCGCTGATGCCGATGTCGATTC
GGCCAAGCTGCTGCAGCGCGCCGGCTATATTCGCAAGGCCGCACCCGGTATCTGGACCTGGCTGCCGCTCGGTCTGCGCG
TGCTGAACAAGATCGAGGCCATCATCCGCGAGGAGATCAACGGCATCGGTGCCCAGGAAGTACACTTCCCGGCCCTGCTG
CCGCGCGAACCCTACGAGGCCACCCATCGCTGGGAGGAGTACGGCGATAACATCTTCCGTTTGAAGGATCGCCACGAGGC
CGATTACCTGCTGGCTCCCACCCACGAGGAGATGTTCACCCTTCTGGTCAAGGACATGTACTCCTCTTACAAGGACCTGC
CCGTAACCCTGTACCAGATCCAGACCAAGTACCGCGACGAGTTCCGCCCGCGCGCCGGCCTGATCCGTGGCCGCGAATTC
ATCATGAAGGACGCCTACTCCTTCACCATTGATGAAGAAGGCATGCGCAAGGCCTACTATGACGAGCGCGGCGCTTACGA
GCGCATCTTCCAGCGTCTCGACCTGAAGTACGTACCTGTGTTCGCCATGTCCGGCCCGATGGGTGGCTCCGCATCCGAGG
AGTTCCTGGCTCCGATGCCGATCGGCGAAGATACCTTCGCGCTGGCGCCTTCCGGCAAGGCATGGAATGTTGAGGCCCTG
AGCACTCCGGAGCTACCGGAAATCGATGCTTCCGCCACTCCCGCCGCTTCCAAGGAAGCCACCCCGGATGCCAAGACCAT
CGATAACATGATCGAGCGTGCCAACGCCGACCACCCGCGTACTGATGGCCGTGAATGGCAGGCTTCCGACATCCTCAAGA
ACGTGGTGATCACCGTCAAGCACCCTGAGGATGAAGAGCATGACGAGCCGTGGCGTGAAGTGATTGTCGTCGGCGTGCCC
GGCGACCGTACGGTGGATATGAAGCGACTGGAAGCCCAGTTCGCTCCCGCCGAGCTTGAGGAGGCCACTGAAGAAGACCT
GAAGCAGCACCCCGAACTGGTGCCAGGCTACATCGGACCGATGGTGCTTGGCCCGCAGGCCGAGGCTGCTGGCGTGAAGA
ACCCGGTTCGCTACCTGGTCGACGCGCACGTGGTCAAGGGTTCCGCTTGGTTCACTGGTGCCGATGAGAACGAGGTCGAC
TACTACAACCTGGTGTACGGCCGTGACTTCAAGGCCGACGGCGTGGTGGAAGCCGTTGAGGTTCGCCACGGCGACATGAG
CCCGGACGGTTCCGGTCCGTTGAGCTTCGAGCGTGGTGTGGAAATCGGCCAGGTGTTCCAGCTGGGTCTCAAGTACTCCA
AGGCACTGGACCTGAAGGTGCTCGACCAGAACGGCAAGGCCGTGCCGGTGTGGATGGGCTGCTATGGCATCGGTGTTTCC
CGCGTGCTGGCCTGCATCGCCGAAACACACCACGACGAAGCCGGCTTGGCTTGGCCGTCCGTGATTGCCCCGGCTGCCGT
GCACGTGGTGGCCACCGGTAAGGACGCCGTGGCGTTTGAAGGTGCCGAGAAGCTGGTCTCCGAGCTTGAGGCCAAGGGCC
TTGAGGTTATTTACGATGACCGCAAGAAGGTGTCTCCGGGCGTCAAGTTCAAGGATGCCGAGCTGATCGGCGTGCCGCTG
GTCGCCGTGGTCGGCCGCGATTACGTCAACGACGGCACCATCGAACTGCGCGACCGCAACGGCGAAAACAAGGTCGCCGT
GCCCGCCGCCGAAGCCGCCGACGCGCTCGCCGAGCGCTTCGCCGCCCTCAGCTGA

Upstream 100 bases:

>100_bases
GCCGTCATTGTTGCGCGAGTGAGTCCGAATACCAAGATGAAGACCGGAATAGTGCGAATAGCAGGCGGGCTTGTCGGTTC
ACGCGAATATCTTGGAACAC

Downstream 100 bases:

>100_bases
GTCCCACTTACTCAGCCTCGGCGTTAATCGCATCAAGCGCCATTCTGCTCAGTCGCAGAATGGCGCTTTGTTGTGGATAA
CCGAAATTTATCCACATTCT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 604; Mature: 603

Protein sequence:

>604_residues
MTSNTLRMSTMFLRTLREDPADADVDSAKLLQRAGYIRKAAPGIWTWLPLGLRVLNKIEAIIREEINGIGAQEVHFPALL
PREPYEATHRWEEYGDNIFRLKDRHEADYLLAPTHEEMFTLLVKDMYSSYKDLPVTLYQIQTKYRDEFRPRAGLIRGREF
IMKDAYSFTIDEEGMRKAYYDERGAYERIFQRLDLKYVPVFAMSGPMGGSASEEFLAPMPIGEDTFALAPSGKAWNVEAL
STPELPEIDASATPAASKEATPDAKTIDNMIERANADHPRTDGREWQASDILKNVVITVKHPEDEEHDEPWREVIVVGVP
GDRTVDMKRLEAQFAPAELEEATEEDLKQHPELVPGYIGPMVLGPQAEAAGVKNPVRYLVDAHVVKGSAWFTGADENEVD
YYNLVYGRDFKADGVVEAVEVRHGDMSPDGSGPLSFERGVEIGQVFQLGLKYSKALDLKVLDQNGKAVPVWMGCYGIGVS
RVLACIAETHHDEAGLAWPSVIAPAAVHVVATGKDAVAFEGAEKLVSELEAKGLEVIYDDRKKVSPGVKFKDAELIGVPL
VAVVGRDYVNDGTIELRDRNGENKVAVPAAEAADALAERFAALS

Sequences:

>Translated_604_residues
MTSNTLRMSTMFLRTLREDPADADVDSAKLLQRAGYIRKAAPGIWTWLPLGLRVLNKIEAIIREEINGIGAQEVHFPALL
PREPYEATHRWEEYGDNIFRLKDRHEADYLLAPTHEEMFTLLVKDMYSSYKDLPVTLYQIQTKYRDEFRPRAGLIRGREF
IMKDAYSFTIDEEGMRKAYYDERGAYERIFQRLDLKYVPVFAMSGPMGGSASEEFLAPMPIGEDTFALAPSGKAWNVEAL
STPELPEIDASATPAASKEATPDAKTIDNMIERANADHPRTDGREWQASDILKNVVITVKHPEDEEHDEPWREVIVVGVP
GDRTVDMKRLEAQFAPAELEEATEEDLKQHPELVPGYIGPMVLGPQAEAAGVKNPVRYLVDAHVVKGSAWFTGADENEVD
YYNLVYGRDFKADGVVEAVEVRHGDMSPDGSGPLSFERGVEIGQVFQLGLKYSKALDLKVLDQNGKAVPVWMGCYGIGVS
RVLACIAETHHDEAGLAWPSVIAPAAVHVVATGKDAVAFEGAEKLVSELEAKGLEVIYDDRKKVSPGVKFKDAELIGVPL
VAVVGRDYVNDGTIELRDRNGENKVAVPAAEAADALAERFAALS
>Mature_603_residues
TSNTLRMSTMFLRTLREDPADADVDSAKLLQRAGYIRKAAPGIWTWLPLGLRVLNKIEAIIREEINGIGAQEVHFPALLP
REPYEATHRWEEYGDNIFRLKDRHEADYLLAPTHEEMFTLLVKDMYSSYKDLPVTLYQIQTKYRDEFRPRAGLIRGREFI
MKDAYSFTIDEEGMRKAYYDERGAYERIFQRLDLKYVPVFAMSGPMGGSASEEFLAPMPIGEDTFALAPSGKAWNVEALS
TPELPEIDASATPAASKEATPDAKTIDNMIERANADHPRTDGREWQASDILKNVVITVKHPEDEEHDEPWREVIVVGVPG
DRTVDMKRLEAQFAPAELEEATEEDLKQHPELVPGYIGPMVLGPQAEAAGVKNPVRYLVDAHVVKGSAWFTGADENEVDY
YNLVYGRDFKADGVVEAVEVRHGDMSPDGSGPLSFERGVEIGQVFQLGLKYSKALDLKVLDQNGKAVPVWMGCYGIGVSR
VLACIAETHHDEAGLAWPSVIAPAAVHVVATGKDAVAFEGAEKLVSELEAKGLEVIYDDRKKVSPGVKFKDAELIGVPLV
AVVGRDYVNDGTIELRDRNGENKVAVPAAEAADALAERFAALS

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=233, Percent_Identity=40.7725321888412, Blast_Score=185, Evalue=8e-47,
Organism=Escherichia coli, GI1786392, Length=587, Percent_Identity=40.0340715502555, Blast_Score=391, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI115532348, Length=211, Percent_Identity=37.4407582938389, Blast_Score=139, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6320931, Length=201, Percent_Identity=43.2835820895522, Blast_Score=172, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24656200, Length=228, Percent_Identity=34.6491228070175, Blast_Score=149, Evalue=5e-36,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 66916; Mature: 66785

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSNTLRMSTMFLRTLREDPADADVDSAKLLQRAGYIRKAAPGIWTWLPLGLRVLNKIEA
CCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHH
IIREEINGIGAQEVHFPALLPREPYEATHRWEEYGDNIFRLKDRHEADYLLAPTHEEMFT
HHHHHHCCCCCCCEECCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCEEECCCHHHHHH
LLVKDMYSSYKDLPVTLYQIQTKYRDEFRPRAGLIRGREFIMKDAYSFTIDEEGMRKAYY
HHHHHHHHHHCCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEEECHHHHHHHHH
DERGAYERIFQRLDLKYVPVFAMSGPMGGSASEEFLAPMPIGEDTFALAPSGKAWNVEAL
HCCCHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEEEEC
STPELPEIDASATPAASKEATPDAKTIDNMIERANADHPRTDGREWQASDILKNVVITVK
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHEEEEEE
HPEDEEHDEPWREVIVVGVPGDRTVDMKRLEAQFAPAELEEATEEDLKQHPELVPGYIGP
CCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCC
MVLGPQAEAAGVKNPVRYLVDAHVVKGSAWFTGADENEVDYYNLVYGRDFKADGVVEAVE
EEECCCCHHCCCCHHHHHHHHHHEEECCEEECCCCCCCCCEEEEEECCCCCCCCCCEEHH
VRHGDMSPDGSGPLSFERGVEIGQVFQLGLKYSKALDLKVLDQNGKAVPVWMGCYGIGVS
HHCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHCCEEEEECCCCCEEEEEHHHHHHHHH
RVLACIAETHHDEAGLAWPSVIAPAAVHVVATGKDAVAFEGAEKLVSELEAKGLEVIYDD
HHHHHHHHHCCCCCCCCCHHHHCCCEEEEEECCCCCEECCCHHHHHHHHHHCCCEEEECC
RKKVSPGVKFKDAELIGVPLVAVVGRDYVNDGTIELRDRNGENKVAVPAAEAADALAERF
CHHCCCCCEECCCCEECCHHHHHHCCCCCCCCEEEEECCCCCCEEEECCHHHHHHHHHHH
AALS
HCCC
>Mature Secondary Structure 
TSNTLRMSTMFLRTLREDPADADVDSAKLLQRAGYIRKAAPGIWTWLPLGLRVLNKIEA
CCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHH
IIREEINGIGAQEVHFPALLPREPYEATHRWEEYGDNIFRLKDRHEADYLLAPTHEEMFT
HHHHHHCCCCCCCEECCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCEEECCCHHHHHH
LLVKDMYSSYKDLPVTLYQIQTKYRDEFRPRAGLIRGREFIMKDAYSFTIDEEGMRKAYY
HHHHHHHHHHCCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEEECHHHHHHHHH
DERGAYERIFQRLDLKYVPVFAMSGPMGGSASEEFLAPMPIGEDTFALAPSGKAWNVEAL
HCCCHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEEEEC
STPELPEIDASATPAASKEATPDAKTIDNMIERANADHPRTDGREWQASDILKNVVITVK
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHEEEEEE
HPEDEEHDEPWREVIVVGVPGDRTVDMKRLEAQFAPAELEEATEEDLKQHPELVPGYIGP
CCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCC
MVLGPQAEAAGVKNPVRYLVDAHVVKGSAWFTGADENEVDYYNLVYGRDFKADGVVEAVE
EEECCCCHHCCCCHHHHHHHHHHEEECCEEECCCCCCCCCEEEEEECCCCCCCCCCEEHH
VRHGDMSPDGSGPLSFERGVEIGQVFQLGLKYSKALDLKVLDQNGKAVPVWMGCYGIGVS
HHCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHCCEEEEECCCCCEEEEEHHHHHHHHH
RVLACIAETHHDEAGLAWPSVIAPAAVHVVATGKDAVAFEGAEKLVSELEAKGLEVIYDD
HHHHHHHHHCCCCCCCCCHHHHCCCEEEEEECCCCCEECCCHHHHHHHHHHCCCEEEECC
RKKVSPGVKFKDAELIGVPLVAVVGRDYVNDGTIELRDRNGENKVAVPAAEAADALAERF
CHHCCCCCEECCCCEECCHHHHHHCCCCCCCCEEEEECCCCCCEEEECCHHHHHHHHHHH
AALS
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12381787 [H]