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The map label for this gene is aspA [H]

Identifier: 213691244

GI number: 213691244

Start: 379229

End: 380662

Strand: Reverse

Name: aspA [H]

Synonym: Blon_0327

Alternate gene names: 213691244

Gene position: 380662-379229 (Counterclockwise)

Preceding gene: 213691245

Following gene: 213691243

Centisome position: 13.44

GC content: 63.04

Gene sequence:

>1434_bases
ATGAGCGAAACATCGAACAAAACCCGTCTGGAACATGACTGCATCGGCCAGATGGAGGTGCCGGCGAACGTCTACTGGGG
CATTCACACCCAGCGCGCTATCGGCAACTTCCCGGTCTCCGGCATCACCGATAGCCAACATCCCGAGCTGATTCGCGCCT
ATGCCACGGTCAAGCGCGCCTGCGCCATCGCCAATGAGGAATTAGGCCTGATCGACCCGGCCAAGGCCGAGGCCATTCGC
TCCGCCTGCCTGGAAATCGAGGCCGGCAAACTGGCCGATCAGTTCCCGGTCGATGTGATGCAGGGCGGTGCCGGCACCTC
GTCGAACATGAACATGAACGAGGTGATCGCCAACCGTGCGCTGGAAATCGCCGGCCACCAGCGCGGCGATTACACATACA
TCCATCCGAACGACGACGTCAACGAGTCGCAGTCCACGAACGACACCTACCCGGCCGCCTGCAAACTCGCGCTTATCGAC
GCGATCGGGCCGCTCGCCGAGTCCACCAAGAAACTCGCCAAGGCCTTCCACGATCTGGCCGACAAACATATCAATGACGT
GACGATCGGCCGTACGCAACTGCAGGACGCCGTGCCGATGACCTATGGTCAGGAGTTTCATTCGTTCGCCACGCTGCTGA
AATCCGATTTGGCCGCGTTCGACCGCGTGGTGCCGTTGCTGGCCCAGCTCAACCTCGGTGCCACCGCCATCGGTACCGGC
ATTTGCGCGGATCTGCGGTTCCGCAAGTCCGCCATCAAGCATCTGGCGCACATCACCGGGCTGCCAGTCACCGCCGCGCC
CGATCCGGTGGCCGCCATGACGGATATGGGCGCGTACGTGGCCACGTCGGCTGCCATCAAGAGCCTCGCCGTGCACCTGA
AGAAGGCCGCCGACGATTTGCGACTGCTCAATTCCGGGCCGCGCTGCGGTTTCAACGATCTCAACGTGCCGGCTCGCCAG
GCCGGATCGTCGATTATGCCGGCCAAAGTGAACCCGGTGATCCCCGAATGCGTCAACCAGTGCTGCTTCACGATCTTCGG
CATGGACGTGACCGTGAATTGGGCCGTGGCCGAGGGACAGTTGCAACTGAACGCCTTCGACCCGGTGATGGTGCACGAGC
TGTTGACCGGCATGGCCCTGTTGACGCGGGCCATGGAGACATTCCGCGTCAATTGCGTGGAGGGCATCGAGATCCACGTC
GGTCTGGGACGCTCGTATGCCCAATCCTCGCCGTCGATCGCCGCCGCACTCAACCACTACATCGGCTATGAGCATGCGGC
CGACATCGCCGCCGAAGCCGTGCGCACCGGTCGCACCGTGCGCGAGGTGGCCGGCGAACGCACCGATCTGCCCGCCGAGC
AACTGGATGAGATTCTCGACCCGATTCGTTTGGCCAGGGGCCTCGGACAGACCTGCCGCGAGCATCGGGACTAG

Upstream 100 bases:

>100_bases
TGGATTGCTTCCGTCGAATGAACCCTGCGTGAGGTTTCTCACAAGGCGCAATCAGCCTCTCATGCTGGCGTTTCCAGCCC
TCACACAGGAAGATGGAAGC

Downstream 100 bases:

>100_bases
GCGTCACGGCGACGTCATCACCACGACGATGCTGCACCGCGATACCGGTGGTGCCACCGGCGGCACGCCACCGACGGCAC
CACCGGTATCGCACCACCAG

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MSETSNKTRLEHDCIGQMEVPANVYWGIHTQRAIGNFPVSGITDSQHPELIRAYATVKRACAIANEELGLIDPAKAEAIR
SACLEIEAGKLADQFPVDVMQGGAGTSSNMNMNEVIANRALEIAGHQRGDYTYIHPNDDVNESQSTNDTYPAACKLALID
AIGPLAESTKKLAKAFHDLADKHINDVTIGRTQLQDAVPMTYGQEFHSFATLLKSDLAAFDRVVPLLAQLNLGATAIGTG
ICADLRFRKSAIKHLAHITGLPVTAAPDPVAAMTDMGAYVATSAAIKSLAVHLKKAADDLRLLNSGPRCGFNDLNVPARQ
AGSSIMPAKVNPVIPECVNQCCFTIFGMDVTVNWAVAEGQLQLNAFDPVMVHELLTGMALLTRAMETFRVNCVEGIEIHV
GLGRSYAQSSPSIAAALNHYIGYEHAADIAAEAVRTGRTVREVAGERTDLPAEQLDEILDPIRLARGLGQTCREHRD

Sequences:

>Translated_477_residues
MSETSNKTRLEHDCIGQMEVPANVYWGIHTQRAIGNFPVSGITDSQHPELIRAYATVKRACAIANEELGLIDPAKAEAIR
SACLEIEAGKLADQFPVDVMQGGAGTSSNMNMNEVIANRALEIAGHQRGDYTYIHPNDDVNESQSTNDTYPAACKLALID
AIGPLAESTKKLAKAFHDLADKHINDVTIGRTQLQDAVPMTYGQEFHSFATLLKSDLAAFDRVVPLLAQLNLGATAIGTG
ICADLRFRKSAIKHLAHITGLPVTAAPDPVAAMTDMGAYVATSAAIKSLAVHLKKAADDLRLLNSGPRCGFNDLNVPARQ
AGSSIMPAKVNPVIPECVNQCCFTIFGMDVTVNWAVAEGQLQLNAFDPVMVHELLTGMALLTRAMETFRVNCVEGIEIHV
GLGRSYAQSSPSIAAALNHYIGYEHAADIAAEAVRTGRTVREVAGERTDLPAEQLDEILDPIRLARGLGQTCREHRD
>Mature_476_residues
SETSNKTRLEHDCIGQMEVPANVYWGIHTQRAIGNFPVSGITDSQHPELIRAYATVKRACAIANEELGLIDPAKAEAIRS
ACLEIEAGKLADQFPVDVMQGGAGTSSNMNMNEVIANRALEIAGHQRGDYTYIHPNDDVNESQSTNDTYPAACKLALIDA
IGPLAESTKKLAKAFHDLADKHINDVTIGRTQLQDAVPMTYGQEFHSFATLLKSDLAAFDRVVPLLAQLNLGATAIGTGI
CADLRFRKSAIKHLAHITGLPVTAAPDPVAAMTDMGAYVATSAAIKSLAVHLKKAADDLRLLNSGPRCGFNDLNVPARQA
GSSIMPAKVNPVIPECVNQCCFTIFGMDVTVNWAVAEGQLQLNAFDPVMVHELLTGMALLTRAMETFRVNCVEGIEIHVG
LGRSYAQSSPSIAAALNHYIGYEHAADIAAEAVRTGRTVREVAGERTDLPAEQLDEILDPIRLARGLGQTCREHRD

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=459, Percent_Identity=38.9978213507625, Blast_Score=320, Evalue=3e-87,
Organism=Escherichia coli, GI87082375, Length=457, Percent_Identity=47.4835886214442, Blast_Score=397, Evalue=1e-111,
Organism=Escherichia coli, GI1787896, Length=467, Percent_Identity=39.8286937901499, Blast_Score=330, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI17553882, Length=456, Percent_Identity=38.5964912280702, Blast_Score=316, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI32565146, Length=320, Percent_Identity=35.9375, Blast_Score=202, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6324993, Length=461, Percent_Identity=39.2624728850325, Blast_Score=325, Evalue=9e-90,
Organism=Drosophila melanogaster, GI24662684, Length=468, Percent_Identity=38.034188034188, Blast_Score=312, Evalue=3e-85,
Organism=Drosophila melanogaster, GI24640179, Length=462, Percent_Identity=37.4458874458874, Blast_Score=311, Evalue=5e-85,
Organism=Drosophila melanogaster, GI24640177, Length=459, Percent_Identity=37.6906318082789, Blast_Score=311, Evalue=5e-85,
Organism=Drosophila melanogaster, GI78710009, Length=463, Percent_Identity=36.9330453563715, Blast_Score=303, Evalue=1e-82,
Organism=Drosophila melanogaster, GI24583245, Length=443, Percent_Identity=35.4401805869075, Blast_Score=278, Evalue=7e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 51311; Mature: 51180

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETSNKTRLEHDCIGQMEVPANVYWGIHTQRAIGNFPVSGITDSQHPELIRAYATVKRA
CCCCCCCCCCHHHHCCCCCCCCEEEECCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
CAIANEELGLIDPAKAEAIRSACLEIEAGKLADQFPVDVMQGGAGTSSNMNMNEVIANRA
HHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHH
LEIAGHQRGDYTYIHPNDDVNESQSTNDTYPAACKLALIDAIGPLAESTKKLAKAFHDLA
HHHCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKHINDVTIGRTQLQDAVPMTYGQEFHSFATLLKSDLAAFDRVVPLLAQLNLGATAIGTG
HHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ICADLRFRKSAIKHLAHITGLPVTAAPDPVAAMTDMGAYVATSAAIKSLAVHLKKAADDL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLLNSGPRCGFNDLNVPARQAGSSIMPAKVNPVIPECVNQCCFTIFGMDVTVNWAVAEGQ
HHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCE
LQLNAFDPVMVHELLTGMALLTRAMETFRVNCVEGIEIHVGLGRSYAQSSPSIAAALNHY
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCEEEEEECCCHHHCCCCHHHHHHHHH
IGYEHAADIAAEAVRTGRTVREVAGERTDLPAEQLDEILDPIRLARGLGQTCREHRD
HCHHHHHHHHHHHHHHCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SETSNKTRLEHDCIGQMEVPANVYWGIHTQRAIGNFPVSGITDSQHPELIRAYATVKRA
CCCCCCCCCHHHHCCCCCCCCEEEECCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
CAIANEELGLIDPAKAEAIRSACLEIEAGKLADQFPVDVMQGGAGTSSNMNMNEVIANRA
HHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHH
LEIAGHQRGDYTYIHPNDDVNESQSTNDTYPAACKLALIDAIGPLAESTKKLAKAFHDLA
HHHCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKHINDVTIGRTQLQDAVPMTYGQEFHSFATLLKSDLAAFDRVVPLLAQLNLGATAIGTG
HHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ICADLRFRKSAIKHLAHITGLPVTAAPDPVAAMTDMGAYVATSAAIKSLAVHLKKAADDL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLLNSGPRCGFNDLNVPARQAGSSIMPAKVNPVIPECVNQCCFTIFGMDVTVNWAVAEGQ
HHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCE
LQLNAFDPVMVHELLTGMALLTRAMETFRVNCVEGIEIHVGLGRSYAQSSPSIAAALNHY
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCEEEEEECCCHHHCCCCHHHHHHHHH
IGYEHAADIAAEAVRTGRTVREVAGERTDLPAEQLDEILDPIRLARGLGQTCREHRD
HCHHHHHHHHHHHHHHCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]