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The map label for this gene is ppa [H]

Identifier: 213691199

GI number: 213691199

Start: 334240

End: 334734

Strand: Reverse

Name: ppa [H]

Synonym: Blon_0281

Alternate gene names: 213691199

Gene position: 334734-334240 (Counterclockwise)

Preceding gene: 213691200

Following gene: 213691198

Centisome position: 11.82

GC content: 61.01

Gene sequence:

>495_bases
ATGGCAGAAACCTTCAACGTCGTTGTGGAAATTCCGCGCGGCTCCAAGAACAAGTACGAAGTCGATCACGAGACCGGTCG
CGTCTTCCTGGACCGTACCCTGTTCACCGCAATGGGTTACCCGGATGACTACGGCTACATCGACGGCACCCTGGGCGAGG
ACGGCGATCCGCTGGACGCTCTCGTGATGATTCCGAACTCCGTGTTCCCGGGCTGCGTGGTCGAGTGCCGCGCCGTGGGC
CTGTACCACATGGTCGACGAGGCCGGCGGCGACGACAAGGTGCTGTGCGTGCCCGCCGACGTTCGTTTCGATGACATCAA
GGACATCGACGACGTCAACGAGTACCACAAGGCCGAAATCAAGCACTTCTTCGAGCAGTACAAGGCTCTGGAGCCGGGCA
AGGAAGTCCTGCCGGGCGACTACTGGACCGGTGCTGACGCCGCCGAAAAGGAAATCATTGCCGCGCGTGAGCGTCTCGCG
AACGAAGGCAAGTGA

Upstream 100 bases:

>100_bases
TCTCAGCACGGTTTCTTGAGTTCCGCACCTGTTTTGCAGGCGCAAACAGGGTATTGTGGTGAGACGATAACGTAACTGTC
GATTTTCAAGGAGAAATGAC

Downstream 100 bases:

>100_bases
TTCGATTCCGAATCGTCGGCGGGAGACCTCTCCGGAGGTCTCCCGCCTTTTTGGTTCGACGTGCATCGCGTGATATAGTG
CTGGTGTATTTTTCAGCTAG

Product: Inorganic diphosphatase

Products: NA

Alternate protein names: Pyrophosphate phospho-hydrolase; PPase [H]

Number of amino acids: Translated: 164; Mature: 163

Protein sequence:

>164_residues
MAETFNVVVEIPRGSKNKYEVDHETGRVFLDRTLFTAMGYPDDYGYIDGTLGEDGDPLDALVMIPNSVFPGCVVECRAVG
LYHMVDEAGGDDKVLCVPADVRFDDIKDIDDVNEYHKAEIKHFFEQYKALEPGKEVLPGDYWTGADAAEKEIIAARERLA
NEGK

Sequences:

>Translated_164_residues
MAETFNVVVEIPRGSKNKYEVDHETGRVFLDRTLFTAMGYPDDYGYIDGTLGEDGDPLDALVMIPNSVFPGCVVECRAVG
LYHMVDEAGGDDKVLCVPADVRFDDIKDIDDVNEYHKAEIKHFFEQYKALEPGKEVLPGDYWTGADAAEKEIIAARERLA
NEGK
>Mature_163_residues
AETFNVVVEIPRGSKNKYEVDHETGRVFLDRTLFTAMGYPDDYGYIDGTLGEDGDPLDALVMIPNSVFPGCVVECRAVGL
YHMVDEAGGDDKVLCVPADVRFDDIKDIDDVNEYHKAEIKHFFEQYKALEPGKEVLPGDYWTGADAAEKEIIAARERLAN
EGK

Specific function: Unknown

COG id: COG0221

COG function: function code C; Inorganic pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790673, Length=166, Percent_Identity=48.1927710843374, Blast_Score=145, Evalue=2e-36,

Paralogues:

None

Copy number: 5480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008162 [H]

Pfam domain/function: PF00719 Pyrophosphatase [H]

EC number: =3.6.1.1 [H]

Molecular weight: Translated: 18281; Mature: 18149

Theoretical pI: Translated: 4.18; Mature: 4.18

Prosite motif: PS00387 PPASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETFNVVVEIPRGSKNKYEVDHETGRVFLDRTLFTAMGYPDDYGYIDGTLGEDGDPLDA
CCCCEEEEEECCCCCCCCEEECCCCCEEEEHHHHHHHCCCCCCCCEECCCCCCCCCCCEE
LVMIPNSVFPGCVVECRAVGLYHMVDEAGGDDKVLCVPADVRFDDIKDIDDVNEYHKAEI
EEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHH
KHFFEQYKALEPGKEVLPGDYWTGADAAEKEIIAARERLANEGK
HHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AETFNVVVEIPRGSKNKYEVDHETGRVFLDRTLFTAMGYPDDYGYIDGTLGEDGDPLDA
CCCEEEEEECCCCCCCCEEECCCCCEEEEHHHHHHHCCCCCCCCEECCCCCCCCCCCEE
LVMIPNSVFPGCVVECRAVGLYHMVDEAGGDDKVLCVPADVRFDDIKDIDDVNEYHKAEI
EEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHH
KHFFEQYKALEPGKEVLPGDYWTGADAAEKEIIAARERLANEGK
HHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]