Click here to switch to the map view.

The map label for this gene is purR [H]

Identifier: 213691186

GI number: 213691186

Start: 314772

End: 315920

Strand: Reverse

Name: purR [H]

Synonym: Blon_0266

Alternate gene names: 213691186

Gene position: 315920-314772 (Counterclockwise)

Preceding gene: 213691187

Following gene: 213691177

Centisome position: 11.15

GC content: 64.58

Gene sequence:

>1149_bases
GTGGGCGCCCACCCGAGAAAAGAGCCACCTGTGAGTGAAAGAACGACATCGGCCGTCGCCGACAAACCCACCCCCACCAT
GCGCGACGTCGCGCAGCTCGCCGGGGTGTCGGTGACCTCGGTGTCCAACTATCTTTCCGATTACCCGTATATGAAGCAGA
CTACCCGCGAACGCATCCGGCAGGCCATCGAGATGCTGGGATATGTAACCGACGAACGGGCACGCAGCCTGCGCTCAGGG
AGAACCGGGCTGATATCCCTGGCCATTCCCAGTCTCACCCAGATATATTTCGCCGAGCTCGCCGAACGCGTGATTGCCGC
GGCACGGCGACGCGGATACGGCGTAACCGTGGAATCCACGGCCTTCAGTCGGCGACATGAGTTGGAAAGCGTCGCCGCCA
TGGCGCGCAAGATGAGTGACGGGTTGATTCTGAGCCCCCTGCTCATGGAACGCCGCGACATCGCCCGCCTGGCCGGCGAC
TATCCGCTCGTCGTGCTCGGCGAGTGCCTGTTTGACGTGCCCGCGCCCCATATCCTCGTGCATAACATCGACGCGGTCAA
GGCGCTGACCCAGCATCTGCTCGACGCGGGTTGCCGGCGCATCGCGCTGATCGGCGGCGCGCTCGACCGACGACGGACCT
CAACCGAAACGCTGCGTACGCAGGGATATCTCGAAGCATTGGCACAGGCCGACGTGCCAGTGGATGCCAAGCTCATCAGG
CCCATCCCCGCCTGGGAGACCGACGAGGAGGCCAAGGACGATGCCAGCGGCGGCGCAGGCTATGTGGGACGGGGCATCGC
CACCAGACGCAACGGTTCGGAAATCGTGCGTCGCATGTTCGAGGAAGGCATCAAGGCCGACGCCCTTCTGTGCTACAACG
ACCTGCTCGCCTTCGGTGCGTTGCGACAACTGCGCGAGATGGGCGTGCGCATCCCGGACGACCTGCGCGTGGTCAGCATG
GACAACCTCGAGGAGGCGCAATACACGATTCCCTCGCTGACCTCCGTCGAGCTTGGCAAGGAGCAGATCGCGCAATGCGC
GGTCGACGCCATTCTGGAGCAAGTGGCCGGCGGCGGGCGCGGCGCAGCCGAGCAGCGTTACGTCGATTTCTCGATCCACT
ACCGGGAGAGCTCGCCGGCGGTGGCGTGA

Upstream 100 bases:

>100_bases
TCCGTGTCCGATGCGTTCCGGCATCGGACACGGAGCCCTTCGACGTTTCGGGAGAACGGGACGGCCGTCGTTATGATGGG
AGGAAACGCACCGCCGGACG

Downstream 100 bases:

>100_bases
TCGACGCACCGGAAACGCCGATGGGGCCCGCGGAATAATCTCCGCGGTCCCCATCATCGCATCATTATCGGTAAGGCATC
GGACTTCCGAGCGGCAACCT

Product: periplasmic binding protein/LacI transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MGAHPRKEPPVSERTTSAVADKPTPTMRDVAQLAGVSVTSVSNYLSDYPYMKQTTRERIRQAIEMLGYVTDERARSLRSG
RTGLISLAIPSLTQIYFAELAERVIAAARRRGYGVTVESTAFSRRHELESVAAMARKMSDGLILSPLLMERRDIARLAGD
YPLVVLGECLFDVPAPHILVHNIDAVKALTQHLLDAGCRRIALIGGALDRRRTSTETLRTQGYLEALAQADVPVDAKLIR
PIPAWETDEEAKDDASGGAGYVGRGIATRRNGSEIVRRMFEEGIKADALLCYNDLLAFGALRQLREMGVRIPDDLRVVSM
DNLEEAQYTIPSLTSVELGKEQIAQCAVDAILEQVAGGGRGAAEQRYVDFSIHYRESSPAVA

Sequences:

>Translated_382_residues
MGAHPRKEPPVSERTTSAVADKPTPTMRDVAQLAGVSVTSVSNYLSDYPYMKQTTRERIRQAIEMLGYVTDERARSLRSG
RTGLISLAIPSLTQIYFAELAERVIAAARRRGYGVTVESTAFSRRHELESVAAMARKMSDGLILSPLLMERRDIARLAGD
YPLVVLGECLFDVPAPHILVHNIDAVKALTQHLLDAGCRRIALIGGALDRRRTSTETLRTQGYLEALAQADVPVDAKLIR
PIPAWETDEEAKDDASGGAGYVGRGIATRRNGSEIVRRMFEEGIKADALLCYNDLLAFGALRQLREMGVRIPDDLRVVSM
DNLEEAQYTIPSLTSVELGKEQIAQCAVDAILEQVAGGGRGAAEQRYVDFSIHYRESSPAVA
>Mature_381_residues
GAHPRKEPPVSERTTSAVADKPTPTMRDVAQLAGVSVTSVSNYLSDYPYMKQTTRERIRQAIEMLGYVTDERARSLRSGR
TGLISLAIPSLTQIYFAELAERVIAAARRRGYGVTVESTAFSRRHELESVAAMARKMSDGLILSPLLMERRDIARLAGDY
PLVVLGECLFDVPAPHILVHNIDAVKALTQHLLDAGCRRIALIGGALDRRRTSTETLRTQGYLEALAQADVPVDAKLIRP
IPAWETDEEAKDDASGGAGYVGRGIATRRNGSEIVRRMFEEGIKADALLCYNDLLAFGALRQLREMGVRIPDDLRVVSMD
NLEEAQYTIPSLTSVELGKEQIAQCAVDAILEQVAGGGRGAAEQRYVDFSIHYRESSPAVA

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=363, Percent_Identity=25.068870523416, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1789068, Length=338, Percent_Identity=28.1065088757396, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1790194, Length=332, Percent_Identity=25.3012048192771, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1787948, Length=341, Percent_Identity=26.6862170087977, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1788474, Length=334, Percent_Identity=23.3532934131737, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI48994940, Length=341, Percent_Identity=24.9266862170088, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1789202, Length=312, Percent_Identity=24.0384615384615, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1786540, Length=339, Percent_Identity=27.4336283185841, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1787906, Length=285, Percent_Identity=24.9122807017544, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787580, Length=345, Percent_Identity=22.6086956521739, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 41867; Mature: 41736

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAHPRKEPPVSERTTSAVADKPTPTMRDVAQLAGVSVTSVSNYLSDYPYMKQTTRERIR
CCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHH
QAIEMLGYVTDERARSLRSGRTGLISLAIPSLTQIYFAELAERVIAAARRRGYGVTVEST
HHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHH
AFSRRHELESVAAMARKMSDGLILSPLLMERRDIARLAGDYPLVVLGECLFDVPAPHILV
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEE
HNIDAVKALTQHLLDAGCRRIALIGGALDRRRTSTETLRTQGYLEALAQADVPVDAKLIR
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHC
PIPAWETDEEAKDDASGGAGYVGRGIATRRNGSEIVRRMFEEGIKADALLCYNDLLAFGA
CCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LRQLREMGVRIPDDLRVVSMDNLEEAQYTIPSLTSVELGKEQIAQCAVDAILEQVAGGGR
HHHHHHCCCCCCCCCEEEECCCCHHHHHCCCCCHHHHHCHHHHHHHHHHHHHHHHCCCCC
GAAEQRYVDFSIHYRESSPAVA
CCCCCEEEEEEEEEECCCCCCC
>Mature Secondary Structure 
GAHPRKEPPVSERTTSAVADKPTPTMRDVAQLAGVSVTSVSNYLSDYPYMKQTTRERIR
CCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHH
QAIEMLGYVTDERARSLRSGRTGLISLAIPSLTQIYFAELAERVIAAARRRGYGVTVEST
HHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHH
AFSRRHELESVAAMARKMSDGLILSPLLMERRDIARLAGDYPLVVLGECLFDVPAPHILV
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEE
HNIDAVKALTQHLLDAGCRRIALIGGALDRRRTSTETLRTQGYLEALAQADVPVDAKLIR
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHC
PIPAWETDEEAKDDASGGAGYVGRGIATRRNGSEIVRRMFEEGIKADALLCYNDLLAFGA
CCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LRQLREMGVRIPDDLRVVSMDNLEEAQYTIPSLTSVELGKEQIAQCAVDAILEQVAGGGR
HHHHHHCCCCCCCCCEEEECCCCHHHHHCCCCCHHHHHCHHHHHHHHHHHHHHHHCCCCC
GAAEQRYVDFSIHYRESSPAVA
CCCCCEEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA