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The map label for this gene is purR [H]

Identifier: 213691097

GI number: 213691097

Start: 210203

End: 210832

Strand: Reverse

Name: purR [H]

Synonym: Blon_0176

Alternate gene names: 213691097

Gene position: 210832-210203 (Counterclockwise)

Preceding gene: 213691098

Following gene: 213691092

Centisome position: 7.44

GC content: 60.48

Gene sequence:

>630_bases
ATGACCGCGAACGAGCCAGCGGAATCATCATCCGCCTCAATCGTCAATGTTGCCGCCATCGCGAATGTTTCCACGGCCAC
GGTATCCCGTGTGCTTTCCGGCAAACGCACCAAGGACGACGACATCGCCCGCCGCGTGCGAGCCGCGGCTCACAGCCTCA
ATTATTCGGTGAATTTTGCGGCCAGTGCGCTGCGTTCCACTGTGACGAGAACCATCGGCCTGATTGTGCCGAGCGTTCAG
GATCCGTTCAGCGCACGGCTCATCGATACGCTTGAGCCGCTGGTCAACAGGAGCGGCCGGCAATTAGCGCTGAGCGTCGG
CAGAACGCCGGAAGTGCAGAAGGAACGCATTGTGTTGATGGCCGCGCGCCATGTGGATGGACTGATTGTCGTGCCACTGC
CCGGCATTGATTTGGGCGATGTGCTGGCGCAATATGCGCAGGAGATTCCTGCCGTGCAAATCGGAGGGTTGCAGCACACC
ACGTTCACGCCTGACTCCGAGCCGGTTCAGCCCGGCCTGCAACAGATTGCCGACGAGCTGCTGCGGCTGCTTGCCGATCA
ATCCAGCACCCCAGTACATGTCAACTTGCCTTCGCGCCTGATTGCTCCGGACTCGCAGGACGCCGAATAA

Upstream 100 bases:

>100_bases
CCAAACGGCCGCCCCGCCTGTCCGGGCGGCCGTCTTTTGTTCTATAGTGATTGCCGGTAATTTCACCGAGCCGGCTTGCT
TTCTGGGAGAGGAGGCACAC

Downstream 100 bases:

>100_bases
ATCGGGCGGCGGGAAGAACCTTACGGAATCAGCGCGCAGAACAGCGATTCAAACACCAGCAACGGGTTGCCATTGCCGGC
AAGCCGCTTGCGGGTGTGCG

Product: regulatory protein, LacI

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MTANEPAESSSASIVNVAAIANVSTATVSRVLSGKRTKDDDIARRVRAAAHSLNYSVNFAASALRSTVTRTIGLIVPSVQ
DPFSARLIDTLEPLVNRSGRQLALSVGRTPEVQKERIVLMAARHVDGLIVVPLPGIDLGDVLAQYAQEIPAVQIGGLQHT
TFTPDSEPVQPGLQQIADELLRLLADQSSTPVHVNLPSRLIAPDSQDAE

Sequences:

>Translated_209_residues
MTANEPAESSSASIVNVAAIANVSTATVSRVLSGKRTKDDDIARRVRAAAHSLNYSVNFAASALRSTVTRTIGLIVPSVQ
DPFSARLIDTLEPLVNRSGRQLALSVGRTPEVQKERIVLMAARHVDGLIVVPLPGIDLGDVLAQYAQEIPAVQIGGLQHT
TFTPDSEPVQPGLQQIADELLRLLADQSSTPVHVNLPSRLIAPDSQDAE
>Mature_208_residues
TANEPAESSSASIVNVAAIANVSTATVSRVLSGKRTKDDDIARRVRAAAHSLNYSVNFAASALRSTVTRTIGLIVPSVQD
PFSARLIDTLEPLVNRSGRQLALSVGRTPEVQKERIVLMAARHVDGLIVVPLPGIDLGDVLAQYAQEIPAVQIGGLQHTT
FTPDSEPVQPGLQQIADELLRLLADQSSTPVHVNLPSRLIAPDSQDAE

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=121, Percent_Identity=34.7107438016529, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1787906, Length=175, Percent_Identity=33.1428571428571, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1789202, Length=136, Percent_Identity=32.3529411764706, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1790194, Length=121, Percent_Identity=31.404958677686, Blast_Score=60, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 22223; Mature: 22092

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTANEPAESSSASIVNVAAIANVSTATVSRVLSGKRTKDDDIARRVRAAAHSLNYSVNFA
CCCCCCCCCCCCCEEEHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHH
ASALRSTVTRTIGLIVPSVQDPFSARLIDTLEPLVNRSGRQLALSVGRTPEVQKERIVLM
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHEE
AARHVDGLIVVPLPGIDLGDVLAQYAQEIPAVQIGGLQHTTFTPDSEPVQPGLQQIADEL
EECCCCCEEEEECCCCCHHHHHHHHHHHCCCEEECCEEECCCCCCCCCCHHHHHHHHHHH
LRLLADQSSTPVHVNLPSRLIAPDSQDAE
HHHHHCCCCCCEEEECCCCEECCCCCCCC
>Mature Secondary Structure 
TANEPAESSSASIVNVAAIANVSTATVSRVLSGKRTKDDDIARRVRAAAHSLNYSVNFA
CCCCCCCCCCCCEEEHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHH
ASALRSTVTRTIGLIVPSVQDPFSARLIDTLEPLVNRSGRQLALSVGRTPEVQKERIVLM
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHEE
AARHVDGLIVVPLPGIDLGDVLAQYAQEIPAVQIGGLQHTTFTPDSEPVQPGLQQIADEL
EECCCCCEEEEECCCCCHHHHHHHHHHHCCCEEECCEEECCCCCCCCCCHHHHHHHHHHH
LRLLADQSSTPVHVNLPSRLIAPDSQDAE
HHHHHCCCCCCEEEECCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA