Click here to switch to the map view.

The map label for this gene is leuA [H]

Identifier: 213691091

GI number: 213691091

Start: 198954

End: 200870

Strand: Direct

Name: leuA [H]

Synonym: Blon_0170

Alternate gene names: 213691091

Gene position: 198954-200870 (Clockwise)

Preceding gene: 213691088

Following gene: 213691093

Centisome position: 7.02

GC content: 62.13

Gene sequence:

>1917_bases
ATGGGTCAGGATCAATCATCCGTTTTTGATCTCGCAGCGGTAGCCGCAGCGTCCAATGGGGGGAACAACGACCCGCTGCT
GCCTCCGGCGCGATTCATCGGCGATCCGCAGAAGCCGAGTCGTATGCCGTACAACAAGTACGCCTCATACAGCGAGCAGA
TCCCGTTTGATTACCCGGAGCGCACGTGGCCGGGCAAGCGACTGCAGCGCGCGCCGCGCTGGTGCTCCGTCGATCTTCGC
GACGGCAATCAGGCCCTCGTCAATCCGATGGATTCCGAGCGCAAGCTGCGGTTCTGGAATCTGCTTGTCTCCATGGGATT
CAAGGAGATCGAGGTGGGCTTCCCGTCCGCTTCCGAGACCGATTTCGACTTCATCCGTATGCTCATCGAGCGTGAGCTTA
TCCCGGACGACGTGACCATCGTGGTGCTCACCCAATGCCGCGAGCACCTCATCCGCCGCACATACGAGGCGCTGAAGGGT
GCCAAGCGCGCCATCGTGCACTTCTACAACTCCGTGTCTGTGCTGCAGCGCGAGGTCGTGTTCCGCAAGAACAAGGAAGA
GATCAAGAAGCTCGCCACCGACGCCGCCGAACTGTGCAAGGACCTCGAGAACGAGGCCAAGGGCATCGACCTGTACTACG
AGTACTCGCCGGAATCCTTCACCGGAACCGAGCCGGAGTACGCCGTCGAGGTGTGCAACGCCGTGATCGGTGTTATCAAG
CCGACTCCCGAGCACCCGATGATCATCAATCTGCCCGCCACCGTGGAAATGACCACGCCGAACGTGTTCGCCGACGAAGT
GGAGTACGTCTCCACCCACCTCGACGACCGCGATTCCGTGGTGCTCTCCCTGCACCCGCACAACGACGAAGGCATGGGCG
TGGCTGCCACCGAGCTGGCCGTGTTGGCCGGCGCCGACCGCGTGGAAGGCTGCCTGCTGGGCAACGGCGAGCGTACCGGC
AACGTCGACTTGGTCACGCTGGGCCTCAACTGGCTCACCCAAGGCATCGACCCGCAGCTTGACCTGTCCAACGTGCCCGA
GATCCGCAAGACGGTCGAATACTGCAACCAGATCAAGATCTCCGAGCGTCACCCGTACGCCGGCAACTTCGTGTTCACCG
CGTTCTCCGGCTCGCATCAGGACGCCATCAAGAAGGGTCTCGAGGCTCGTCAGGTGGCCGCCGAGCGCGCTGGCGCTGAT
CTTGACAGCTTCGTGTGGCTCGTGCCGTACCTGCCGATCGACCCGAAGGACATCGGCCGCACGTACGAGGCCATCATCCG
CGTCAACTCGCAGTCCGGCAAGGGCGGCATGGCCTACCTGCTCAAGACCAACCACAATCTTGACCTGCCCAAGCGTCTTC
AGATCGAATTCGACAAGATCGTGCAGAACTACGCTGACACGACCAAGAAGGAAGTCAAGGACGGGGATATCTGGCGTCTG
TTCAAGGACGAGTATCTGCCGGTCGAACAGTCCGGCATGACCGCGGCCGGTGTGGTTGTGGGCGATACGCATGACGCCTC
GCTTGAGCCGTGGGGTCGGCTGAAGCTGCTCAAGGTGGCCGTCAGCTCCGGGGAGGATGGTTCCGATACCGTGCTCAAGG
CCCGCCTGCTCGACCGCGGCGTGAACGTCGGGGACGATGAGCCCGTGGAGCGCGAGGCGTCCGGCATAGGCAACGGTCCG
ATCGCCGCCTTCCTGAACGCCATTTCCAACTTTGGCGTGGAAGCCTCGATTATGGATTACGTGGAGCACACCATGTCCGT
CGGTACCGACGCCATGGCCGCCTCGTATGTGGAATGCCAGATCGGCGAGGCCGATGACGCGCAGATCGTGTGGGGCGTAG
GCATCGACTCGTCGATTACCACCAGCGCCCTGAAGGCGATCATCTCCGCCATCAACCGTTCTCAGCGTCAGCGCTGA

Upstream 100 bases:

>100_bases
CGTGCGTATTGACAAATCCGCGTGGCCCGGCGCCGCCCTGGCACAACGCCGCAGTCGCGTCCGCAAGAGGGCCCGCGCAA
TAACCAGTAAAGGAGGAACA

Downstream 100 bases:

>100_bases
GCGCTAACAGAGCAAAACGAAGCTCCCTCTGATTGCGTCAGAGGGAGCTTCGTTGTCTGTGTCTACTTACTACTTGACGC
CGTTGGTGTCGGTGTTCCCG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 638; Mature: 637

Protein sequence:

>638_residues
MGQDQSSVFDLAAVAAASNGGNNDPLLPPARFIGDPQKPSRMPYNKYASYSEQIPFDYPERTWPGKRLQRAPRWCSVDLR
DGNQALVNPMDSERKLRFWNLLVSMGFKEIEVGFPSASETDFDFIRMLIERELIPDDVTIVVLTQCREHLIRRTYEALKG
AKRAIVHFYNSVSVLQREVVFRKNKEEIKKLATDAAELCKDLENEAKGIDLYYEYSPESFTGTEPEYAVEVCNAVIGVIK
PTPEHPMIINLPATVEMTTPNVFADEVEYVSTHLDDRDSVVLSLHPHNDEGMGVAATELAVLAGADRVEGCLLGNGERTG
NVDLVTLGLNWLTQGIDPQLDLSNVPEIRKTVEYCNQIKISERHPYAGNFVFTAFSGSHQDAIKKGLEARQVAAERAGAD
LDSFVWLVPYLPIDPKDIGRTYEAIIRVNSQSGKGGMAYLLKTNHNLDLPKRLQIEFDKIVQNYADTTKKEVKDGDIWRL
FKDEYLPVEQSGMTAAGVVVGDTHDASLEPWGRLKLLKVAVSSGEDGSDTVLKARLLDRGVNVGDDEPVEREASGIGNGP
IAAFLNAISNFGVEASIMDYVEHTMSVGTDAMAASYVECQIGEADDAQIVWGVGIDSSITTSALKAIISAINRSQRQR

Sequences:

>Translated_638_residues
MGQDQSSVFDLAAVAAASNGGNNDPLLPPARFIGDPQKPSRMPYNKYASYSEQIPFDYPERTWPGKRLQRAPRWCSVDLR
DGNQALVNPMDSERKLRFWNLLVSMGFKEIEVGFPSASETDFDFIRMLIERELIPDDVTIVVLTQCREHLIRRTYEALKG
AKRAIVHFYNSVSVLQREVVFRKNKEEIKKLATDAAELCKDLENEAKGIDLYYEYSPESFTGTEPEYAVEVCNAVIGVIK
PTPEHPMIINLPATVEMTTPNVFADEVEYVSTHLDDRDSVVLSLHPHNDEGMGVAATELAVLAGADRVEGCLLGNGERTG
NVDLVTLGLNWLTQGIDPQLDLSNVPEIRKTVEYCNQIKISERHPYAGNFVFTAFSGSHQDAIKKGLEARQVAAERAGAD
LDSFVWLVPYLPIDPKDIGRTYEAIIRVNSQSGKGGMAYLLKTNHNLDLPKRLQIEFDKIVQNYADTTKKEVKDGDIWRL
FKDEYLPVEQSGMTAAGVVVGDTHDASLEPWGRLKLLKVAVSSGEDGSDTVLKARLLDRGVNVGDDEPVEREASGIGNGP
IAAFLNAISNFGVEASIMDYVEHTMSVGTDAMAASYVECQIGEADDAQIVWGVGIDSSITTSALKAIISAINRSQRQR
>Mature_637_residues
GQDQSSVFDLAAVAAASNGGNNDPLLPPARFIGDPQKPSRMPYNKYASYSEQIPFDYPERTWPGKRLQRAPRWCSVDLRD
GNQALVNPMDSERKLRFWNLLVSMGFKEIEVGFPSASETDFDFIRMLIERELIPDDVTIVVLTQCREHLIRRTYEALKGA
KRAIVHFYNSVSVLQREVVFRKNKEEIKKLATDAAELCKDLENEAKGIDLYYEYSPESFTGTEPEYAVEVCNAVIGVIKP
TPEHPMIINLPATVEMTTPNVFADEVEYVSTHLDDRDSVVLSLHPHNDEGMGVAATELAVLAGADRVEGCLLGNGERTGN
VDLVTLGLNWLTQGIDPQLDLSNVPEIRKTVEYCNQIKISERHPYAGNFVFTAFSGSHQDAIKKGLEARQVAAERAGADL
DSFVWLVPYLPIDPKDIGRTYEAIIRVNSQSGKGGMAYLLKTNHNLDLPKRLQIEFDKIVQNYADTTKKEVKDGDIWRLF
KDEYLPVEQSGMTAAGVVVGDTHDASLEPWGRLKLLKVAVSSGEDGSDTVLKARLLDRGVNVGDDEPVEREASGIGNGPI
AAFLNAISNFGVEASIMDYVEHTMSVGTDAMAASYVECQIGEADDAQIVWGVGIDSSITTSALKAIISAINRSQRQR

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=572, Percent_Identity=25.5244755244755, Blast_Score=104, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6324225, Length=591, Percent_Identity=46.1928934010152, Blast_Score=520, Evalue=1e-148,
Organism=Saccharomyces cerevisiae, GI6324682, Length=599, Percent_Identity=44.5742904841402, Blast_Score=499, Evalue=1e-142,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 70506; Mature: 70375

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQDQSSVFDLAAVAAASNGGNNDPLLPPARFIGDPQKPSRMPYNKYASYSEQIPFDYPE
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCC
RTWPGKRLQRAPRWCSVDLRDGNQALVNPMDSERKLRFWNLLVSMGFKEIEVGFPSASET
CCCCCHHHHCCCCEEEEEECCCCHHHCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCC
DFDFIRMLIERELIPDDVTIVVLTQCREHLIRRTYEALKGAKRAIVHFYNSVSVLQREVV
CHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRKNKEEIKKLATDAAELCKDLENEAKGIDLYYEYSPESFTGTEPEYAVEVCNAVIGVIK
HHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCC
PTPEHPMIINLPATVEMTTPNVFADEVEYVSTHLDDRDSVVLSLHPHNDEGMGVAATELA
CCCCCCEEEECCCEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHH
VLAGADRVEGCLLGNGERTGNVDLVTLGLNWLTQGIDPQLDLSNVPEIRKTVEYCNQIKI
HHCCCCHHCEEEECCCCCCCCEEEEEECHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCEE
SERHPYAGNFVFTAFSGSHQDAIKKGLEARQVAAERAGADLDSFVWLVPYLPIDPKDIGR
CCCCCCCCCEEEEEECCCCHHHHHHCHHHHHHHHHHCCCCHHHHHHEECCCCCCHHHHHH
TYEAIIRVNSQSGKGGMAYLLKTNHNLDLPKRLQIEFDKIVQNYADTTKKEVKDGDIWRL
HEEEHEEECCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
FKDEYLPVEQSGMTAAGVVVGDTHDASLEPWGRLKLLKVAVSSGEDGSDTVLKARLLDRG
HHHCCCCCCCCCCEEEEEEEECCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHCC
VNVGDDEPVEREASGIGNGPIAAFLNAISNFGVEASIMDYVEHTMSVGTDAMAASYVECQ
CCCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHEEE
IGEADDAQIVWGVGIDSSITTSALKAIISAINRSQRQR
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GQDQSSVFDLAAVAAASNGGNNDPLLPPARFIGDPQKPSRMPYNKYASYSEQIPFDYPE
CCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCC
RTWPGKRLQRAPRWCSVDLRDGNQALVNPMDSERKLRFWNLLVSMGFKEIEVGFPSASET
CCCCCHHHHCCCCEEEEEECCCCHHHCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCC
DFDFIRMLIERELIPDDVTIVVLTQCREHLIRRTYEALKGAKRAIVHFYNSVSVLQREVV
CHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRKNKEEIKKLATDAAELCKDLENEAKGIDLYYEYSPESFTGTEPEYAVEVCNAVIGVIK
HHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCC
PTPEHPMIINLPATVEMTTPNVFADEVEYVSTHLDDRDSVVLSLHPHNDEGMGVAATELA
CCCCCCEEEECCCEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHH
VLAGADRVEGCLLGNGERTGNVDLVTLGLNWLTQGIDPQLDLSNVPEIRKTVEYCNQIKI
HHCCCCHHCEEEECCCCCCCCEEEEEECHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCEE
SERHPYAGNFVFTAFSGSHQDAIKKGLEARQVAAERAGADLDSFVWLVPYLPIDPKDIGR
CCCCCCCCCEEEEEECCCCHHHHHHCHHHHHHHHHHCCCCHHHHHHEECCCCCCHHHHHH
TYEAIIRVNSQSGKGGMAYLLKTNHNLDLPKRLQIEFDKIVQNYADTTKKEVKDGDIWRL
HEEEHEEECCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
FKDEYLPVEQSGMTAAGVVVGDTHDASLEPWGRLKLLKVAVSSGEDGSDTVLKARLLDRG
HHHCCCCCCCCCCEEEEEEEECCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHCC
VNVGDDEPVEREASGIGNGPIAAFLNAISNFGVEASIMDYVEHTMSVGTDAMAASYVECQ
CCCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHEEE
IGEADDAQIVWGVGIDSSITTSALKAIISAINRSQRQR
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA