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The map label for this gene is phoA [H]

Identifier: 213691061

GI number: 213691061

Start: 166830

End: 168908

Strand: Direct

Name: phoA [H]

Synonym: Blon_0140

Alternate gene names: 213691061

Gene position: 166830-168908 (Clockwise)

Preceding gene: 213691057

Following gene: 213691062

Centisome position: 5.89

GC content: 66.62

Gene sequence:

>2079_bases
ATGGGCAATCACAAGGCTTTGACGAGCGCCGTAGCGGCGATCGCCTCCATTGCGACGCTCGGCGCGCTCGCCGCTCCGGC
GTTTGCTGTCGATGCCGCGAACGGCACCTACAGCCCCAACGGCAAGTCGGTCGCCGAGCTCGCCAAGCACGGCGCGGCGC
AGCGCATCGCCGCCATCGGCAACAAGAGCGCGAAGAACGTCGTGCTGTTCATCGGCGACGGCATGGGCGATTCGGAAATC
ACCATCGCCCGCGACTATCTGAAAGGCGCCAACGGCCACTTCGATGGTCTGGACGCGGTCGGTCAGCCCGGCGCGCTTGA
CGACGTGCAGGCGGGCACCGGTCAGTACACGACCTTCTCGCTCGGCGACGGCAGCAAGGACAGCGCCGTCGGCAAGGATG
CCGCCGGCAAGCTGGTCGCCAACCCCAACCCGGGCAAGCTCACCCCCGTCACCGATTCCTCCGCGTCCGGTTCCGCATGG
GCGACCGGCACCAAGACCTACAACAACGCCGTCGACGTCGACATCTACGGCAACCCGCAGCTGAATCTCTTCGAGCTGGC
CAAGGCCGCCGGCAAGGCCACCGGCAACGTGTCCACCGCGGAAATCCAGGACGCCACCCCGGCCGTGCTGGAATCGCACT
CCACCGAGCGTGCCTGCTACGGCCCGCAGGGCAAGACCGACGGCACTTCCAACAACGCCTCCAAACAGTGCCTCGCCAAC
CAGCTCAAGGAGAATGGCGGCATCGGCTCCATCTCCGAGCAGCTGCTCGACACCCGCGCCGATGTGACCATCGGCGGTGG
TTCCAAGTACTTCCGCCAGACCGTGCAGGGCGGCGAATACAAGGGCAAGACCGTGTGGGAGCAGGCCAAGGACATGGGCT
TCCAGACCGTTGAGAACGACCCGGCCGCGATGAACGCCCTGCAGTACAAGGATGGCCAGCCGGTGCTCGCCCTGATGAGC
GACGGCAACATGCCGACCAAGTTCAACCCGTCCAAGGCCACCGCCAAGGATCCGGCCAAGGACGCCAACCCGACCGTGTG
CACCCCGAATGACGCCTGGCTCGGCAACCGTGGCGCCTCGCTCAAGGACATGACCAAGAAGGCGCTCGATCTGCTCAACG
CCAACCCGAACGGTCAGAAGAACGGCTTCCTCCTGCAGGTCGAAGGCGCTTCGATCGATAAGCAGGACCACGCCGCCAAC
GCCTGCGGCCAGATCGGCGAGACCGACGACTTCGACCAGGCCATCGCCTACGCCATCAAGAACGTGGACCTCACGAACAC
GCTGGTGATCGTCACCGCCGATCATGCGCACACCTCCCAGGTCCTCAACGCCCAGCCGGCCTACGCGCTGTCCACCGTGC
TCAAGTCCGCCGACGGCAACAACATGGTCGTCTCCTACGGCACCGCCCAGGCAGCCTCCACCGACGCCGAAGGCGGCTAC
AACGGCGGCGACATGGAGCACACCGGCACCCAGCTGCGCATCGCCGCTTCCGGCCCCGGCGCCCAGCGCGTCATCGGCCT
GACCGATCAGACCGACAACTTCTACACCATCGCCGGCGCCCTGGGCCTGGCCACCTCCCCCGACGAGCAGAACGCGCTCT
CCAAGGATGGCACCGTGAAGGTCGCCCGCACGCAGGCCGGCAAGTACGTCGCCAATGTCGACGGGTTCAACGGCGACGCG
CTGCTCAGCTACGAGCTGAAGGACAAGAACGGCGCCGTGGTGTCCAAGTCCGATTCCAGCACCCCGTTGTCCGGCGTGCG
CGTCAAGCCCGCGCAGACCACTTCGATCGCGCTCGACAAGATGTCGGAAGGCGCCGAGTACACGCTCACCGTCACCGGTC
GCCAGTCCGGCAAGGTCGCGACCGTCGCCATCCAGGCGCCGGCCGCCGGCTCCGCCGACAAGAAGCCGGGCGCCGACAAG
AACGGCGTGATCGCTTCGGGCAAGGTGCCGAACGATTCCGCGGTCTCCGGCCCGCTGGGCGCCACCGGTACCGCCGTCAT
GGCCATCGCCATCGCGGCTGTGATGCTGGTTGCGATCGCGATGCTCATTAAGGCCGCGCGTAACGCCAGGAACAACTGA

Upstream 100 bases:

>100_bases
AGCTGGAAGGCGTGACGACGACGGACGTGGCCAAACGGGTGCGGCCGGAGGAGTGCGGGCCGGACGGCGCAGATGACACT
GCATGGAAGAAGGAAGAACA

Downstream 100 bases:

>100_bases
TTCGGCCTTGGTCGACCGGTAATCCCGAGTCGTCCAGCCGCCGGAAAACCATCGCCCGCCGAGCCGATGCCCGGCGGGCG
ATCGTTTTCCGGCGGCTTTT

Product: alkaline phosphatase

Products: NA

Alternate protein names: APase [H]

Number of amino acids: Translated: 692; Mature: 691

Protein sequence:

>692_residues
MGNHKALTSAVAAIASIATLGALAAPAFAVDAANGTYSPNGKSVAELAKHGAAQRIAAIGNKSAKNVVLFIGDGMGDSEI
TIARDYLKGANGHFDGLDAVGQPGALDDVQAGTGQYTTFSLGDGSKDSAVGKDAAGKLVANPNPGKLTPVTDSSASGSAW
ATGTKTYNNAVDVDIYGNPQLNLFELAKAAGKATGNVSTAEIQDATPAVLESHSTERACYGPQGKTDGTSNNASKQCLAN
QLKENGGIGSISEQLLDTRADVTIGGGSKYFRQTVQGGEYKGKTVWEQAKDMGFQTVENDPAAMNALQYKDGQPVLALMS
DGNMPTKFNPSKATAKDPAKDANPTVCTPNDAWLGNRGASLKDMTKKALDLLNANPNGQKNGFLLQVEGASIDKQDHAAN
ACGQIGETDDFDQAIAYAIKNVDLTNTLVIVTADHAHTSQVLNAQPAYALSTVLKSADGNNMVVSYGTAQAASTDAEGGY
NGGDMEHTGTQLRIAASGPGAQRVIGLTDQTDNFYTIAGALGLATSPDEQNALSKDGTVKVARTQAGKYVANVDGFNGDA
LLSYELKDKNGAVVSKSDSSTPLSGVRVKPAQTTSIALDKMSEGAEYTLTVTGRQSGKVATVAIQAPAAGSADKKPGADK
NGVIASGKVPNDSAVSGPLGATGTAVMAIAIAAVMLVAIAMLIKAARNARNN

Sequences:

>Translated_692_residues
MGNHKALTSAVAAIASIATLGALAAPAFAVDAANGTYSPNGKSVAELAKHGAAQRIAAIGNKSAKNVVLFIGDGMGDSEI
TIARDYLKGANGHFDGLDAVGQPGALDDVQAGTGQYTTFSLGDGSKDSAVGKDAAGKLVANPNPGKLTPVTDSSASGSAW
ATGTKTYNNAVDVDIYGNPQLNLFELAKAAGKATGNVSTAEIQDATPAVLESHSTERACYGPQGKTDGTSNNASKQCLAN
QLKENGGIGSISEQLLDTRADVTIGGGSKYFRQTVQGGEYKGKTVWEQAKDMGFQTVENDPAAMNALQYKDGQPVLALMS
DGNMPTKFNPSKATAKDPAKDANPTVCTPNDAWLGNRGASLKDMTKKALDLLNANPNGQKNGFLLQVEGASIDKQDHAAN
ACGQIGETDDFDQAIAYAIKNVDLTNTLVIVTADHAHTSQVLNAQPAYALSTVLKSADGNNMVVSYGTAQAASTDAEGGY
NGGDMEHTGTQLRIAASGPGAQRVIGLTDQTDNFYTIAGALGLATSPDEQNALSKDGTVKVARTQAGKYVANVDGFNGDA
LLSYELKDKNGAVVSKSDSSTPLSGVRVKPAQTTSIALDKMSEGAEYTLTVTGRQSGKVATVAIQAPAAGSADKKPGADK
NGVIASGKVPNDSAVSGPLGATGTAVMAIAIAAVMLVAIAMLIKAARNARNN
>Mature_691_residues
GNHKALTSAVAAIASIATLGALAAPAFAVDAANGTYSPNGKSVAELAKHGAAQRIAAIGNKSAKNVVLFIGDGMGDSEIT
IARDYLKGANGHFDGLDAVGQPGALDDVQAGTGQYTTFSLGDGSKDSAVGKDAAGKLVANPNPGKLTPVTDSSASGSAWA
TGTKTYNNAVDVDIYGNPQLNLFELAKAAGKATGNVSTAEIQDATPAVLESHSTERACYGPQGKTDGTSNNASKQCLANQ
LKENGGIGSISEQLLDTRADVTIGGGSKYFRQTVQGGEYKGKTVWEQAKDMGFQTVENDPAAMNALQYKDGQPVLALMSD
GNMPTKFNPSKATAKDPAKDANPTVCTPNDAWLGNRGASLKDMTKKALDLLNANPNGQKNGFLLQVEGASIDKQDHAANA
CGQIGETDDFDQAIAYAIKNVDLTNTLVIVTADHAHTSQVLNAQPAYALSTVLKSADGNNMVVSYGTAQAASTDAEGGYN
GGDMEHTGTQLRIAASGPGAQRVIGLTDQTDNFYTIAGALGLATSPDEQNALSKDGTVKVARTQAGKYVANVDGFNGDAL
LSYELKDKNGAVVSKSDSSTPLSGVRVKPAQTTSIALDKMSEGAEYTLTVTGRQSGKVATVAIQAPAAGSADKKPGADKN
GVIASGKVPNDSAVSGPLGATGTAVMAIAIAAVMLVAIAMLIKAARNARNN

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI94721246, Length=522, Percent_Identity=28.1609195402299, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI157266296, Length=520, Percent_Identity=27.3076923076923, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI157266292, Length=411, Percent_Identity=28.2238442822384, Blast_Score=94, Evalue=4e-19,
Organism=Escherichia coli, GI48994877, Length=469, Percent_Identity=48.6140724946695, Blast_Score=380, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6320689, Length=468, Percent_Identity=25.8547008547009, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21355151, Length=506, Percent_Identity=23.3201581027668, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI18859923, Length=384, Percent_Identity=25, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI21355981, Length=405, Percent_Identity=24.4444444444444, Blast_Score=77, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24657835, Length=441, Percent_Identity=25.8503401360544, Blast_Score=77, Evalue=5e-14,
Organism=Drosophila melanogaster, GI21358067, Length=521, Percent_Identity=24.9520153550864, Blast_Score=75, Evalue=2e-13,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 70606; Mature: 70475

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNHKALTSAVAAIASIATLGALAAPAFAVDAANGTYSPNGKSVAELAKHGAAQRIAAIG
CCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHCCHHHHHHHHC
NKSAKNVVLFIGDGMGDSEITIARDYLKGANGHFDGLDAVGQPGALDDVQAGTGQYTTFS
CCCCCEEEEEEECCCCCCCEEEEHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
LGDGSKDSAVGKDAAGKLVANPNPGKLTPVTDSSASGSAWATGTKTYNNAVDVDIYGNPQ
CCCCCCCCCCCCCCCCCEEECCCCCCEEECCCCCCCCCEEECCCCCCCCEEEEEEECCCC
LNLFELAKAAGKATGNVSTAEIQDATPAVLESHSTERACYGPQGKTDGTSNNASKQCLAN
CHHHHHHHHHCCCCCCCCEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
QLKENGGIGSISEQLLDTRADVTIGGGSKYFRQTVQGGEYKGKTVWEQAKDMGFQTVEND
HHHHCCCCCHHHHHHHHCCCCEEECCCHHHHHHHHCCCCCCCCHHHHHHHHCCCEEECCC
PAAMNALQYKDGQPVLALMSDGNMPTKFNPSKATAKDPAKDANPTVCTPNDAWLGNRGAS
CHHHHHEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCC
LKDMTKKALDLLNANPNGQKNGFLLQVEGASIDKQDHAANACGQIGETDDFDQAIAYAIK
HHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHH
NVDLTNTLVIVTADHAHTSQVLNAQPAYALSTVLKSADGNNMVVSYGTAQAASTDAEGGY
CCCCCCEEEEEEECCCHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCC
NGGDMEHTGTQLRIAASGPGAQRVIGLTDQTDNFYTIAGALGLATSPDEQNALSKDGTVK
CCCCCCCCCCEEEEEECCCCCCEEEEECCCCCCEEEEEEECCCCCCCCHHHCCCCCCCEE
VARTQAGKYVANVDGFNGDALLSYELKDKNGAVVSKSDSSTPLSGVRVKPAQTTSIALDK
EEEECCCCEEEECCCCCCCEEEEEEEECCCCCEEECCCCCCCCCCEEECCCCCHHHHHHH
MSEGAEYTLTVTGRQSGKVATVAIQAPAAGSADKKPGADKNGVIASGKVPNDSAVSGPLG
HCCCCEEEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCC
ATGTAVMAIAIAAVMLVAIAMLIKAARNARNN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GNHKALTSAVAAIASIATLGALAAPAFAVDAANGTYSPNGKSVAELAKHGAAQRIAAIG
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHCCHHHHHHHHC
NKSAKNVVLFIGDGMGDSEITIARDYLKGANGHFDGLDAVGQPGALDDVQAGTGQYTTFS
CCCCCEEEEEEECCCCCCCEEEEHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
LGDGSKDSAVGKDAAGKLVANPNPGKLTPVTDSSASGSAWATGTKTYNNAVDVDIYGNPQ
CCCCCCCCCCCCCCCCCEEECCCCCCEEECCCCCCCCCEEECCCCCCCCEEEEEEECCCC
LNLFELAKAAGKATGNVSTAEIQDATPAVLESHSTERACYGPQGKTDGTSNNASKQCLAN
CHHHHHHHHHCCCCCCCCEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
QLKENGGIGSISEQLLDTRADVTIGGGSKYFRQTVQGGEYKGKTVWEQAKDMGFQTVEND
HHHHCCCCCHHHHHHHHCCCCEEECCCHHHHHHHHCCCCCCCCHHHHHHHHCCCEEECCC
PAAMNALQYKDGQPVLALMSDGNMPTKFNPSKATAKDPAKDANPTVCTPNDAWLGNRGAS
CHHHHHEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCC
LKDMTKKALDLLNANPNGQKNGFLLQVEGASIDKQDHAANACGQIGETDDFDQAIAYAIK
HHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHH
NVDLTNTLVIVTADHAHTSQVLNAQPAYALSTVLKSADGNNMVVSYGTAQAASTDAEGGY
CCCCCCEEEEEEECCCHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCC
NGGDMEHTGTQLRIAASGPGAQRVIGLTDQTDNFYTIAGALGLATSPDEQNALSKDGTVK
CCCCCCCCCCEEEEEECCCCCCEEEEECCCCCCEEEEEEECCCCCCCCHHHCCCCCCCEE
VARTQAGKYVANVDGFNGDALLSYELKDKNGAVVSKSDSSTPLSGVRVKPAQTTSIALDK
EEEECCCCEEEECCCCCCCEEEEEEEECCCCCEEECCCCCCCCCCEEECCCCCHHHHHHH
MSEGAEYTLTVTGRQSGKVATVAIQAPAAGSADKKPGADKNGVIASGKVPNDSAVSGPLG
HCCCCEEEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCC
ATGTAVMAIAIAAVMLVAIAMLIKAARNARNN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2129542 [H]