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The map label for this gene is livM [H]

Identifier: 213691027

GI number: 213691027

Start: 127080

End: 128195

Strand: Direct

Name: livM [H]

Synonym: Blon_0106

Alternate gene names: 213691027

Gene position: 127080-128195 (Clockwise)

Preceding gene: 213691026

Following gene: 213691028

Centisome position: 4.49

GC content: 60.84

Gene sequence:

>1116_bases
ATGGGATCTGTTGCCGCATTCGTCACCAAGTACCGTTCATGGATCGGTTCCATCGCCGCCGTCGCGTTGATCATCCTCGC
GCCGACGGTCATGAGCGCGTACAGTCTGGGGCTGTTGTCCAAATATCTGTGTTACGCGATGGTCGCGGTCGGCATTGGCC
TGGCATGGGGGCAGGGAGGCATGCTGACCTTGGGTCAGGGACTGTTCTTCGGACTCGGCGCCTATGTGATGGCCATCCAC
CTGAAGATCGAAGAACTCGGTCCAGACGCGGTTCCCGACTTCATGGCACAGTACGGCATCGACAGACTTCCCGGATTCTG
GGAGCTTTTCCGCAATCCGGTCGTCACCATCATCGGCGTCGCCGTGGTGCCGGGTGCCGTTGCGGCGGCCCTCGGTTGGG
CCGTGTTCGGCAGAGGCGTGCGTGGCGCCTACTTCGCGATCCTTTCCCAAGCGTTGGTCGCCGCGTTCGCCGTGCTGCTT
GTTGGCCAGTCCAAGACCACGGGCGGCACCAATGGCCTGAACGACTTCTCCGGTTTCTTCGGCTACGATTTGACCGACCC
GGCCAACAAAAGGATGATCTACTACATTTGCGCGTTCTGCGTGCTGGGCATGGTGCTCATCGCCGATTGGATTCGTCATT
CATTCATGGGCGAGCTCATCATCGGCGTGCGGGACCAGGAGAACCGTATGAAGTTCCTCGGCTACAATCCCGCTTCCATC
AAAGTGTTCACGTTCTCCCTCGCGGCCGTGTTCGCGGGCATCGGCGGCGCCATGTTCGTACCGGTCGTCGGCATCATCTC
CCCCTCCGACATCGATGTGATTCCATCCATCATGATGCTTGCCGGCGTGGTGATCGGGGGAAGAAGCACCTTGCTTGGTC
CGGTCATCGGCACGCTGATCATTCGATTCGCCGAAACGCAGCTCTCCGAGGCCTGCCCGTCCGTTTGGACGTACGTCGAA
GGCGCGATCTTCATCCTGGTCATCGCCTTCGCCCCGATGGGCCTTGGCCAGATCAGGAACTTCCGTCCATGGCTGGCCGG
AAGGCTCGGCCATGATGGCGTGAAGGCCACGGTGCCGAACGAGGCGGCAACGACCACAAAGGCGGTGAACGCATGA

Upstream 100 bases:

>100_bases
CATTTTCCTGATCGTGGTGATCTTCCTGCAGTTCCGTCCTCAAGGTCTGGTCGCGGTCCGTTCCAGGAATCTGGCATAAG
GCAAGAAGGGAGTGCATGTT

Downstream 100 bases:

>100_bases
GCGACGAAAACAAGACCGAGGGCGCCGAGCTGGATCAGCTGCGCGATGAGATGCGCAGGATGCGCGATGAAGCCAGACGC
TTCCGCGATGAGGCCGCCAG

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MGSVAAFVTKYRSWIGSIAAVALIILAPTVMSAYSLGLLSKYLCYAMVAVGIGLAWGQGGMLTLGQGLFFGLGAYVMAIH
LKIEELGPDAVPDFMAQYGIDRLPGFWELFRNPVVTIIGVAVVPGAVAAALGWAVFGRGVRGAYFAILSQALVAAFAVLL
VGQSKTTGGTNGLNDFSGFFGYDLTDPANKRMIYYICAFCVLGMVLIADWIRHSFMGELIIGVRDQENRMKFLGYNPASI
KVFTFSLAAVFAGIGGAMFVPVVGIISPSDIDVIPSIMMLAGVVIGGRSTLLGPVIGTLIIRFAETQLSEACPSVWTYVE
GAIFILVIAFAPMGLGQIRNFRPWLAGRLGHDGVKATVPNEAATTTKAVNA

Sequences:

>Translated_371_residues
MGSVAAFVTKYRSWIGSIAAVALIILAPTVMSAYSLGLLSKYLCYAMVAVGIGLAWGQGGMLTLGQGLFFGLGAYVMAIH
LKIEELGPDAVPDFMAQYGIDRLPGFWELFRNPVVTIIGVAVVPGAVAAALGWAVFGRGVRGAYFAILSQALVAAFAVLL
VGQSKTTGGTNGLNDFSGFFGYDLTDPANKRMIYYICAFCVLGMVLIADWIRHSFMGELIIGVRDQENRMKFLGYNPASI
KVFTFSLAAVFAGIGGAMFVPVVGIISPSDIDVIPSIMMLAGVVIGGRSTLLGPVIGTLIIRFAETQLSEACPSVWTYVE
GAIFILVIAFAPMGLGQIRNFRPWLAGRLGHDGVKATVPNEAATTTKAVNA
>Mature_370_residues
GSVAAFVTKYRSWIGSIAAVALIILAPTVMSAYSLGLLSKYLCYAMVAVGIGLAWGQGGMLTLGQGLFFGLGAYVMAIHL
KIEELGPDAVPDFMAQYGIDRLPGFWELFRNPVVTIIGVAVVPGAVAAALGWAVFGRGVRGAYFAILSQALVAAFAVLLV
GQSKTTGGTNGLNDFSGFFGYDLTDPANKRMIYYICAFCVLGMVLIADWIRHSFMGELIIGVRDQENRMKFLGYNPASIK
VFTFSLAAVFAGIGGAMFVPVVGIISPSDIDVIPSIMMLAGVVIGGRSTLLGPVIGTLIIRFAETQLSEACPSVWTYVEG
AIFILVIAFAPMGLGQIRNFRPWLAGRLGHDGVKATVPNEAATTTKAVNA

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=333, Percent_Identity=24.024024024024, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 39243; Mature: 39112

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSVAAFVTKYRSWIGSIAAVALIILAPTVMSAYSLGLLSKYLCYAMVAVGIGLAWGQGG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
MLTLGQGLFFGLGAYVMAIHLKIEELGPDAVPDFMAQYGIDRLPGFWELFRNPVVTIIGV
CEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHCCHHHHHHH
AVVPGAVAAALGWAVFGRGVRGAYFAILSQALVAAFAVLLVGQSKTTGGTNGLNDFSGFF
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHC
GYDLTDPANKRMIYYICAFCVLGMVLIADWIRHSFMGELIIGVRDQENRMKFLGYNPASI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEECCCCCEE
KVFTFSLAAVFAGIGGAMFVPVVGIISPSDIDVIPSIMMLAGVVIGGRSTLLGPVIGTLI
EEHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
IRFAETQLSEACPSVWTYVEGAIFILVIAFAPMGLGQIRNFRPWLAGRLGHDGVKATVPN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHCCCCCCCEEECCC
EAATTTKAVNA
CHHHCHHHCCC
>Mature Secondary Structure 
GSVAAFVTKYRSWIGSIAAVALIILAPTVMSAYSLGLLSKYLCYAMVAVGIGLAWGQGG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
MLTLGQGLFFGLGAYVMAIHLKIEELGPDAVPDFMAQYGIDRLPGFWELFRNPVVTIIGV
CEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHCCHHHHHHH
AVVPGAVAAALGWAVFGRGVRGAYFAILSQALVAAFAVLLVGQSKTTGGTNGLNDFSGFF
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHC
GYDLTDPANKRMIYYICAFCVLGMVLIADWIRHSFMGELIIGVRDQENRMKFLGYNPASI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEECCCCCEE
KVFTFSLAAVFAGIGGAMFVPVVGIISPSDIDVIPSIMMLAGVVIGGRSTLLGPVIGTLI
EEHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
IRFAETQLSEACPSVWTYVEGAIFILVIAFAPMGLGQIRNFRPWLAGRLGHDGVKATVPN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHCCCCCCCEEECCC
EAATTTKAVNA
CHHHCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]