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The map label for this gene is ngoFVIIM [H]

Identifier: 213691014

GI number: 213691014

Start: 111635

End: 112807

Strand: Reverse

Name: ngoFVIIM [H]

Synonym: Blon_0092

Alternate gene names: 213691014

Gene position: 112807-111635 (Counterclockwise)

Preceding gene: 213691016

Following gene: 213691013

Centisome position: 3.98

GC content: 55.75

Gene sequence:

>1173_bases
ATGACTGGTGCATCCAGCGGGGCTACCCGTATCGGCTCAACCGCACCACAAGCGCTTGAGTTCTTTGCCGGCATAGGGCT
TGCCCGCCTAGGCCTCGAAGAGGCTGGCTTCCACGTGGCGTGGTCGAACGATATAGATCACGCCAAATGCCAGATGTATA
GGAACCAGTTCGGCAACAATGAGCAGGAACATACCCTGATCGAGGGGGACATGGGTGAGCTACATGGCTCTGACTTGCCT
CACAATATCTCCATTGCTTGGGGCTCCTCCCCTTGTACCGATATATCTCTGGCTGGAACCCGTACAGGGTTGAACGGCAA
ACAGTCCAGCGCTTTCTGGCAGTATATCCGCCTCCTGGAAGAGATGGGCAACAATAGGCCACCTGTAGCCGTACTTGAGA
ATGTCGCAGGTCTTGCTACGTCTCACTCAGGCGAGGATATAGCAGCAGCAGTAAGCGCATTCAACCAACTGGGATATTCA
GTTGACGCGCTGTCAATCGACGCTCGGCGTTTTGTGCCCCAGTCCCGCCCCAGACTGTTCTTGGTCGGCCTAACCAATCC
TCCTGAGGGCTCGCAAGCCAATTCCCCACTACGGCCAGATTGGTTGCAACAGGTGTTTGCCGATACCTCCCTGCGTATGC
ACAGGGCAGTTCTGCCGGAACCGCCGGAACCACTTATGGCGGGCTTTGGCATGTACGCCGAAGAGATGTCAGACGATGAT
TCCCGATGGTGGCCTAAGGGCAAGCTAACGGCATTTCTTGACTCACTGTCCGCTGTGCAATCAGAGAGAGTGATGAAGCT
GAAGGACAATCCGTTCATCTCTTATCGAACGGCATACAGGCGCACTCGGAACGGGAAACCCACGTGGGAAGTCCGAGCGG
ATGACATTTCGGGATGTCTACGAACAGCTCGCGGGGGTTCTTCAAGACAAGCCGTGGTACGACTCGGCAAGGGGTCGACG
GCAGTGCGCTGGATGACTCCAAAGGAGTACGCGGCACTTATGGGCGCATCTCATTACAACCTTAATGGTCTGCGCACCAA
CCAAGCCTTGTTCGGCTTCGGTGACGCAGTGGCAGTACCTGTAGTCGAATGGCTTGGCGAGCACTACTTCATGCCAGCAC
TTCGCGGTACATTGGACAAGGCAAAGCAGCAACACGAGCTACGGTCAGCTTGA

Upstream 100 bases:

>100_bases
TCATAGGAGGAGCGCGCCACAGCGAATACGCATACGATTTCATAGCAAGCAGCACGTACACTAGTACCAGTGAATGTCAG
AAATCGGAAAGGAACAGGGC

Downstream 100 bases:

>100_bases
TAGTCGTCGAAGGAGAGCGAGCATGGGTATTGACGCCATCGAGGAGTTTCGAAAGAGCCACGGCATTAACACCAAAGGCA
ACCTAGCCGGCATACTGCAG

Product: DNA-cytosine methyltransferase

Products: NA

Alternate protein names: M.NgoFVII; Cytosine-specific methyltransferase NgoFVII; M.NgoVII [H]

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MTGASSGATRIGSTAPQALEFFAGIGLARLGLEEAGFHVAWSNDIDHAKCQMYRNQFGNNEQEHTLIEGDMGELHGSDLP
HNISIAWGSSPCTDISLAGTRTGLNGKQSSAFWQYIRLLEEMGNNRPPVAVLENVAGLATSHSGEDIAAAVSAFNQLGYS
VDALSIDARRFVPQSRPRLFLVGLTNPPEGSQANSPLRPDWLQQVFADTSLRMHRAVLPEPPEPLMAGFGMYAEEMSDDD
SRWWPKGKLTAFLDSLSAVQSERVMKLKDNPFISYRTAYRRTRNGKPTWEVRADDISGCLRTARGGSSRQAVVRLGKGST
AVRWMTPKEYAALMGASHYNLNGLRTNQALFGFGDAVAVPVVEWLGEHYFMPALRGTLDKAKQQHELRSA

Sequences:

>Translated_390_residues
MTGASSGATRIGSTAPQALEFFAGIGLARLGLEEAGFHVAWSNDIDHAKCQMYRNQFGNNEQEHTLIEGDMGELHGSDLP
HNISIAWGSSPCTDISLAGTRTGLNGKQSSAFWQYIRLLEEMGNNRPPVAVLENVAGLATSHSGEDIAAAVSAFNQLGYS
VDALSIDARRFVPQSRPRLFLVGLTNPPEGSQANSPLRPDWLQQVFADTSLRMHRAVLPEPPEPLMAGFGMYAEEMSDDD
SRWWPKGKLTAFLDSLSAVQSERVMKLKDNPFISYRTAYRRTRNGKPTWEVRADDISGCLRTARGGSSRQAVVRLGKGST
AVRWMTPKEYAALMGASHYNLNGLRTNQALFGFGDAVAVPVVEWLGEHYFMPALRGTLDKAKQQHELRSA
>Mature_389_residues
TGASSGATRIGSTAPQALEFFAGIGLARLGLEEAGFHVAWSNDIDHAKCQMYRNQFGNNEQEHTLIEGDMGELHGSDLPH
NISIAWGSSPCTDISLAGTRTGLNGKQSSAFWQYIRLLEEMGNNRPPVAVLENVAGLATSHSGEDIAAAVSAFNQLGYSV
DALSIDARRFVPQSRPRLFLVGLTNPPEGSQANSPLRPDWLQQVFADTSLRMHRAVLPEPPEPLMAGFGMYAEEMSDDDS
RWWPKGKLTAFLDSLSAVQSERVMKLKDNPFISYRTAYRRTRNGKPTWEVRADDISGCLRTARGGSSRQAVVRLGKGSTA
VRWMTPKEYAALMGASHYNLNGLRTNQALFGFGDAVAVPVVEWLGEHYFMPALRGTLDKAKQQHELRSA

Specific function: This methylase recognizes the double-stranded sequence GCSGC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the NgoFVII endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 42740; Mature: 42608

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGASSGATRIGSTAPQALEFFAGIGLARLGLEEAGFHVAWSNDIDHAKCQMYRNQFGNN
CCCCCCCCCCCCCCCHHHHHHHHHCCHHHHCHHHCCEEEEECCCCCHHHHHHHHHHHCCC
EQEHTLIEGDMGELHGSDLPHNISIAWGSSPCTDISLAGTRTGLNGKQSSAFWQYIRLLE
CCCCEEEECCCHHCCCCCCCCEEEEEECCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHH
EMGNNRPPVAVLENVAGLATSHSGEDIAAAVSAFNQLGYSVDALSIDARRFVPQSRPRLF
HHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEEECHHHCCCCCCCCEE
LVGLTNPPEGSQANSPLRPDWLQQVFADTSLRMHRAVLPEPPEPLMAGFGMYAEEMSDDD
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCC
SRWWPKGKLTAFLDSLSAVQSERVMKLKDNPFISYRTAYRRTRNGKPTWEVRADDISGCL
CCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCEEHHHHHHHCCCCCCCEEEECHHHHHHH
RTARGGSSRQAVVRLGKGSTAVRWMTPKEYAALMGASHYNLNGLRTNQALFGFGDAVAVP
HHHCCCCCCEEEEEECCCCCEEEEECHHHHHHHHCCCCCCCCCEECCCHHHCCCCHHHHH
VVEWLGEHYFMPALRGTLDKAKQQHELRSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TGASSGATRIGSTAPQALEFFAGIGLARLGLEEAGFHVAWSNDIDHAKCQMYRNQFGNN
CCCCCCCCCCCCCCHHHHHHHHHCCHHHHCHHHCCEEEEECCCCCHHHHHHHHHHHCCC
EQEHTLIEGDMGELHGSDLPHNISIAWGSSPCTDISLAGTRTGLNGKQSSAFWQYIRLLE
CCCCEEEECCCHHCCCCCCCCEEEEEECCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHH
EMGNNRPPVAVLENVAGLATSHSGEDIAAAVSAFNQLGYSVDALSIDARRFVPQSRPRLF
HHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEEECHHHCCCCCCCCEE
LVGLTNPPEGSQANSPLRPDWLQQVFADTSLRMHRAVLPEPPEPLMAGFGMYAEEMSDDD
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCC
SRWWPKGKLTAFLDSLSAVQSERVMKLKDNPFISYRTAYRRTRNGKPTWEVRADDISGCL
CCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCEEHHHHHHHCCCCCCCEEEECHHHHHHH
RTARGGSSRQAVVRLGKGSTAVRWMTPKEYAALMGASHYNLNGLRTNQALFGFGDAVAVP
HHHCCCCCCEEEEEECCCCCEEEEECHHHHHHHHCCCCCCCCCEECCCHHHCCCCHHHHH
VVEWLGEHYFMPALRGTLDKAKQQHELRSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7607490 [H]