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The map label for this gene is ccpA [H]

Identifier: 213690966

GI number: 213690966

Start: 46992

End: 48014

Strand: Direct

Name: ccpA [H]

Synonym: Blon_0042

Alternate gene names: 213690966

Gene position: 46992-48014 (Clockwise)

Preceding gene: 213690965

Following gene: 213690967

Centisome position: 1.66

GC content: 55.03

Gene sequence:

>1023_bases
ATGGCGGGCATCAAAGATGTAGCCCAGGAGGCGGGAGTGTCGGTTAGCACCGTTTCGTACGTGCTTTCCGGCAAGCGTTC
CATCTCCGCCAAAACCACCGATAAGGTGATGGCCGCCGTTGAACGACTTGGATATACTCCGGATGCCAGCGCCCGTAAAA
TGCGTGGTATGCGCAATCATATTTTTGCGCTCAGCGCGCCGATTCGCGGTGATATCAATCAGGCCAAATACAACGCTTAC
TTTCTGCAGACCGCATGGCAGGCAAAGAACCGTGGCTACGATGTGCTGTTGCTTACCGGCGAGGACGCGGTGGCCGACAT
TCGGCGCGTGACGCAAAGCAACTTGGTTGATGGCGTGGTGCTGCTCGACATTGAGGAACATGACGAGCGTACCGCCCAGG
CTGGCTCATATTCCAAACCCTGTGTGGCCATCGGTTACCCTGCAGAGCATGAAGGCTGTGCCTGTGTCGATATCGATTTT
GCCACGGCTGGTCGTATGGCTGTGGATTGCCTGTATTCCAAGGGGCATCGGAGTGCTATGTTTTTGCGTGACAATGAGAC
CGATTGCGATCGCGGTTCGGGCTATGTGCTGCTGTTCCGCAAGGCTTTGCTGGAACGTGCCGAAGAGTTGGGCATGACGA
TTCATGAATCCTTCAAGCATCGTGACGATCGCTTTGATGCCGTGGAATTCGTGCGTGAGCTTTGTTCTTTGGATGAGCGC
CCCACGGCGATTGTCAACCAGGCGAACGCCAATGTGCTGAAGTTGGTATTGCAGCAATTACAGGCTTCGGGATTGCGGGT
GCCAGATGATATTTCGGTATTGTCGTGCGGCACGTATTTCGAGGGAGAGCTGATGCCGCAGCCGATTACCGAGATGCCGG
TCATGCCGCAGGAATTATGTTCTAAGGCTGTGGATCTGCTGGTCGACGCCATCGATGAGCGTCGCGACATCAAGGGATTG
GTGGAACTGTCCGCTCCGGTCATGCATAGTCGAGGTTCCGTTACCAAAGTTGGTGACGTGTGA

Upstream 100 bases:

>100_bases
TCCGGGAGACTTGGCGGATGGTTTCGTTGGATGATGAGTGAGGATTGCGGCACGCGCAATGTGTGGCGCAAGCGATTTGG
AGAGCCGAAGGAGGCGGAAG

Downstream 100 bases:

>100_bases
AAAGAAAACTGTAGGAGTATGTCCGTGAAAGGCGGACGGATTTCCGTATCCATAATCGAAACGATTCGAAAGTAAGTGAA
GGCGTTGTAGCCGAAGCGAA

Product: regulatory protein, LacI

Products: NA

Alternate protein names: Glucose-resistance amylase regulator [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MAGIKDVAQEAGVSVSTVSYVLSGKRSISAKTTDKVMAAVERLGYTPDASARKMRGMRNHIFALSAPIRGDINQAKYNAY
FLQTAWQAKNRGYDVLLLTGEDAVADIRRVTQSNLVDGVVLLDIEEHDERTAQAGSYSKPCVAIGYPAEHEGCACVDIDF
ATAGRMAVDCLYSKGHRSAMFLRDNETDCDRGSGYVLLFRKALLERAEELGMTIHESFKHRDDRFDAVEFVRELCSLDER
PTAIVNQANANVLKLVLQQLQASGLRVPDDISVLSCGTYFEGELMPQPITEMPVMPQELCSKAVDLLVDAIDERRDIKGL
VELSAPVMHSRGSVTKVGDV

Sequences:

>Translated_340_residues
MAGIKDVAQEAGVSVSTVSYVLSGKRSISAKTTDKVMAAVERLGYTPDASARKMRGMRNHIFALSAPIRGDINQAKYNAY
FLQTAWQAKNRGYDVLLLTGEDAVADIRRVTQSNLVDGVVLLDIEEHDERTAQAGSYSKPCVAIGYPAEHEGCACVDIDF
ATAGRMAVDCLYSKGHRSAMFLRDNETDCDRGSGYVLLFRKALLERAEELGMTIHESFKHRDDRFDAVEFVRELCSLDER
PTAIVNQANANVLKLVLQQLQASGLRVPDDISVLSCGTYFEGELMPQPITEMPVMPQELCSKAVDLLVDAIDERRDIKGL
VELSAPVMHSRGSVTKVGDV
>Mature_339_residues
AGIKDVAQEAGVSVSTVSYVLSGKRSISAKTTDKVMAAVERLGYTPDASARKMRGMRNHIFALSAPIRGDINQAKYNAYF
LQTAWQAKNRGYDVLLLTGEDAVADIRRVTQSNLVDGVVLLDIEEHDERTAQAGSYSKPCVAIGYPAEHEGCACVDIDFA
TAGRMAVDCLYSKGHRSAMFLRDNETDCDRGSGYVLLFRKALLERAEELGMTIHESFKHRDDRFDAVEFVRELCSLDERP
TAIVNQANANVLKLVLQQLQASGLRVPDDISVLSCGTYFEGELMPQPITEMPVMPQELCSKAVDLLVDAIDERRDIKGLV
ELSAPVMHSRGSVTKVGDV

Specific function: Transcriptional regulator involved in both the repression of carbohydrate utilization genes such as the alpha- amylase (AmyE) and the acetyl-coenzyme A synthetase (AcsA); and in the positive regulation of genes involved in excretion of excess carbon such

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=350, Percent_Identity=28, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787948, Length=343, Percent_Identity=25.3644314868805, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790369, Length=320, Percent_Identity=26.25, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1787580, Length=204, Percent_Identity=28.921568627451, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789068, Length=290, Percent_Identity=26.2068965517241, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789456, Length=330, Percent_Identity=23.3333333333333, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790689, Length=193, Percent_Identity=28.4974093264249, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006377
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37338; Mature: 37207

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGIKDVAQEAGVSVSTVSYVLSGKRSISAKTTDKVMAAVERLGYTPDASARKMRGMRNH
CCCHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
IFALSAPIRGDINQAKYNAYFLQTAWQAKNRGYDVLLLTGEDAVADIRRVTQSNLVDGVV
EEEEECCCCCCCCHHHHHEEEEHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHCCCEE
LLDIEEHDERTAQAGSYSKPCVAIGYPAEHEGCACVDIDFATAGRMAVDCLYSKGHRSAM
EEEEHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEEEECHHCCCHHHHHHHHHCCCCEEE
FLRDNETDCDRGSGYVLLFRKALLERAEELGMTIHESFKHRDDRFDAVEFVRELCSLDER
EEECCCCCCCCCCCEEHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHCCCCC
PTAIVNQANANVLKLVLQQLQASGLRVPDDISVLSCGTYFEGELMPQPITEMPVMPQELC
CCHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCHHCCCCHHHHH
SKAVDLLVDAIDERRDIKGLVELSAPVMHSRGSVTKVGDV
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEECCCC
>Mature Secondary Structure 
AGIKDVAQEAGVSVSTVSYVLSGKRSISAKTTDKVMAAVERLGYTPDASARKMRGMRNH
CCHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
IFALSAPIRGDINQAKYNAYFLQTAWQAKNRGYDVLLLTGEDAVADIRRVTQSNLVDGVV
EEEEECCCCCCCCHHHHHEEEEHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHCCCEE
LLDIEEHDERTAQAGSYSKPCVAIGYPAEHEGCACVDIDFATAGRMAVDCLYSKGHRSAM
EEEEHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEEEECHHCCCHHHHHHHHHCCCCEEE
FLRDNETDCDRGSGYVLLFRKALLERAEELGMTIHESFKHRDDRFDAVEFVRELCSLDER
EEECCCCCCCCCCCEEHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHCCCCC
PTAIVNQANANVLKLVLQQLQASGLRVPDDISVLSCGTYFEGELMPQPITEMPVMPQELC
CCHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCHHCCCCHHHHH
SKAVDLLVDAIDERRDIKGLVELSAPVMHSRGSVTKVGDV
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1904524; 9387221; 9384377 [H]