Click here to switch to the map view.

The map label for this gene is gdh [H]

Identifier: 213690939

GI number: 213690939

Start: 13433

End: 14779

Strand: Reverse

Name: gdh [H]

Synonym: Blon_0011

Alternate gene names: 213690939

Gene position: 14779-13433 (Counterclockwise)

Preceding gene: 213690940

Following gene: 213690938

Centisome position: 0.52

GC content: 63.18

Gene sequence:

>1347_bases
ATGCTCACTGACGAGTACGTCAAGCGCGTCTACAACCAGGTCGTGGCCCGCGATGGCGATCAGCCCGAATTTCTGCAAGC
CGTCTACGAAGTGCTCGACACCCTTCAGCCGGTCGTCGCAAAGCACCCGGAATACGAGAAGAACGGTGTGCTCGAGCGCA
TCGTCGAACCTGAGCGCGTGGTGAAGTTCCGCGTCGCCTGGACCGACGACGAAGGCAAGGTGCAGGTCAACCGTGGTTAC
CGCGTGCAGTTCAATTCCGCCATCGGCCCGTACAAGGGCGGCCTGCGCTTCCACCCGACCGTCAACGAGAGCGTCATCAA
GTTCCTCGGTTTCGAGCAGATCCTGAAGAACTCCCTGACCACGCTGCCGATGGGCGGCGGCAAGGGCGGCTCCGACTTCA
ACCCGAAGGGCAAGTCCGACGCGGAAGTCATGCGCTTCTGCCAGGCCTTCATGACCGAGCTGTGCCGTCACATCGGCCAG
TTCACCGACGTTCCCGCCGGCGACATCAACGTCGGTGGCCGTGAGATCGGCTACCTGTTCGGCCAGTACAAGCGCATCCG
TGACGAATACTCCGGCGTGCTGACCGGCAAGGGCCTCGAATTCGGTGGCTCTCTGGCCCGCACCGAGGCCACCGGTTACG
GCGTGTGCTACTACACCCAGGAAGCCCTGCGCGTGCTGCGCAACGACTCCTTCAAGGGCAAGACCGTCGTGATCTCCGGT
GCCGGCAACGTGGCCATCTACGCCGCGCAGAAGGCCGAGGAGCTGGGCGCCAAGGTCGTGACCGTGTCCGATTCCAACGG
CTATGTCTACGATCCGAACGGCATCGACGTCGCCGTGGTCAAGCAGATCAAGGAGGTCGAGCGCGGCCGTATCAAGGAGT
ACGCCGAGCGCGTTACATCCGCCGAATACCACGAGGGTTGCTCCGGCGTGTGGACCGTGCCGTGCGACATCGCGCTGCCG
TGCGCCACTCAGAACGAAATCGACGAGGAATCCGCCAAGGCTCTTGTAGCCAACGGCTGCAAGGTGGTGTGCGAGGGCGC
CAACATGCCTTCCACCCCCGAAGCCATCGAGGTCTACAAGTCCAATAAGCTGCTGTACGGCCCGGCCAAGGCTGCCAACG
CCGGTGGCGTGGCCGTCTCCGGTCTGGAGATGAGCCAGAACAGCTACCGTCTGAGCTGGACCTTCGAGGAGGTCGACGCC
AAGCTCAAGTCCATCATGGAATCCATCGTCGCCAACTCCCTGGCCGCCGCCAAGGAGTACGGCGAGGAAGGCGATCTGAT
GGCCGGCGCCAACATCGCCGGTTTCGTCAAGGTAGCCAACGCCATGGTCGCCCAGGGCGTGCTGTGA

Upstream 100 bases:

>100_bases
GCGCTGAACGCTTTCCCGTTATCCGAGCGCCCTCTATGCAACACAGGGTCGGGTGCATAGAATAAGAACGTCTTATCTAT
CTATCGAATCGAGGAGAACA

Downstream 100 bases:

>100_bases
CGCTTGCCGCTAAGCAGTACTAAGCAGTAGCGTAATGCCCTTGCTTCCCCGTGAGGAGGCAAGGGCATTGCTATATGTAC
AACGAACGCAACAAGTGCTA

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MLTDEYVKRVYNQVVARDGDQPEFLQAVYEVLDTLQPVVAKHPEYEKNGVLERIVEPERVVKFRVAWTDDEGKVQVNRGY
RVQFNSAIGPYKGGLRFHPTVNESVIKFLGFEQILKNSLTTLPMGGGKGGSDFNPKGKSDAEVMRFCQAFMTELCRHIGQ
FTDVPAGDINVGGREIGYLFGQYKRIRDEYSGVLTGKGLEFGGSLARTEATGYGVCYYTQEALRVLRNDSFKGKTVVISG
AGNVAIYAAQKAEELGAKVVTVSDSNGYVYDPNGIDVAVVKQIKEVERGRIKEYAERVTSAEYHEGCSGVWTVPCDIALP
CATQNEIDEESAKALVANGCKVVCEGANMPSTPEAIEVYKSNKLLYGPAKAANAGGVAVSGLEMSQNSYRLSWTFEEVDA
KLKSIMESIVANSLAAAKEYGEEGDLMAGANIAGFVKVANAMVAQGVL

Sequences:

>Translated_448_residues
MLTDEYVKRVYNQVVARDGDQPEFLQAVYEVLDTLQPVVAKHPEYEKNGVLERIVEPERVVKFRVAWTDDEGKVQVNRGY
RVQFNSAIGPYKGGLRFHPTVNESVIKFLGFEQILKNSLTTLPMGGGKGGSDFNPKGKSDAEVMRFCQAFMTELCRHIGQ
FTDVPAGDINVGGREIGYLFGQYKRIRDEYSGVLTGKGLEFGGSLARTEATGYGVCYYTQEALRVLRNDSFKGKTVVISG
AGNVAIYAAQKAEELGAKVVTVSDSNGYVYDPNGIDVAVVKQIKEVERGRIKEYAERVTSAEYHEGCSGVWTVPCDIALP
CATQNEIDEESAKALVANGCKVVCEGANMPSTPEAIEVYKSNKLLYGPAKAANAGGVAVSGLEMSQNSYRLSWTFEEVDA
KLKSIMESIVANSLAAAKEYGEEGDLMAGANIAGFVKVANAMVAQGVL
>Mature_448_residues
MLTDEYVKRVYNQVVARDGDQPEFLQAVYEVLDTLQPVVAKHPEYEKNGVLERIVEPERVVKFRVAWTDDEGKVQVNRGY
RVQFNSAIGPYKGGLRFHPTVNESVIKFLGFEQILKNSLTTLPMGGGKGGSDFNPKGKSDAEVMRFCQAFMTELCRHIGQ
FTDVPAGDINVGGREIGYLFGQYKRIRDEYSGVLTGKGLEFGGSLARTEATGYGVCYYTQEALRVLRNDSFKGKTVVISG
AGNVAIYAAQKAEELGAKVVTVSDSNGYVYDPNGIDVAVVKQIKEVERGRIKEYAERVTSAEYHEGCSGVWTVPCDIALP
CATQNEIDEESAKALVANGCKVVCEGANMPSTPEAIEVYKSNKLLYGPAKAANAGGVAVSGLEMSQNSYRLSWTFEEVDA
KLKSIMESIVANSLAAAKEYGEEGDLMAGANIAGFVKVANAMVAQGVL

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=436, Percent_Identity=31.1926605504587, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI4885281, Length=437, Percent_Identity=30.6636155606407, Blast_Score=171, Evalue=2e-42,
Organism=Escherichia coli, GI1788059, Length=445, Percent_Identity=56.8539325842697, Blast_Score=505, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI17544676, Length=387, Percent_Identity=29.7157622739018, Blast_Score=144, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6324951, Length=452, Percent_Identity=53.5398230088496, Blast_Score=423, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6319256, Length=456, Percent_Identity=52.4122807017544, Blast_Score=420, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24649283, Length=450, Percent_Identity=30, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI45553475, Length=381, Percent_Identity=30.4461942257218, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI45549226, Length=381, Percent_Identity=30.4461942257218, Blast_Score=148, Evalue=7e-36,
Organism=Drosophila melanogaster, GI24649460, Length=315, Percent_Identity=31.4285714285714, Blast_Score=122, Evalue=6e-28,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 48669; Mature: 48669

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTDEYVKRVYNQVVARDGDQPEFLQAVYEVLDTLQPVVAKHPEYEKNGVLERIVEPERV
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCE
VKFRVAWTDDEGKVQVNRGYRVQFNSAIGPYKGGLRFHPTVNESVIKFLGFEQILKNSLT
EEEEEEEECCCCCEEECCCEEEEECCCCCCCCCCEEECCCCHHHHHHHHCHHHHHHCCCC
TLPMGGGKGGSDFNPKGKSDAEVMRFCQAFMTELCRHIGQFTDVPAGDINVGGREIGYLF
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
GQYKRIRDEYSGVLTGKGLEFGGSLARTEATGYGVCYYTQEALRVLRNDSFKGKTVVISG
HHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHCCCCCCCEEEEEC
AGNVAIYAAQKAEELGAKVVTVSDSNGYVYDPNGIDVAVVKQIKEVERGRIKEYAERVTS
CCCEEEEEEHHHHHCCCEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
AEYHEGCSGVWTVPCDIALPCATQNEIDEESAKALVANGCKVVCEGANMPSTPEAIEVYK
HHHHCCCCCEEEECCCEEECCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHH
SNKLLYGPAKAANAGGVAVSGLEMSQNSYRLSWTFEEVDAKLKSIMESIVANSLAAAKEY
CCEEEECCCCCCCCCCEEEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHC
GEEGDLMAGANIAGFVKVANAMVAQGVL
CCCCCEEECCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLTDEYVKRVYNQVVARDGDQPEFLQAVYEVLDTLQPVVAKHPEYEKNGVLERIVEPERV
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCE
VKFRVAWTDDEGKVQVNRGYRVQFNSAIGPYKGGLRFHPTVNESVIKFLGFEQILKNSLT
EEEEEEEECCCCCEEECCCEEEEECCCCCCCCCCEEECCCCHHHHHHHHCHHHHHHCCCC
TLPMGGGKGGSDFNPKGKSDAEVMRFCQAFMTELCRHIGQFTDVPAGDINVGGREIGYLF
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
GQYKRIRDEYSGVLTGKGLEFGGSLARTEATGYGVCYYTQEALRVLRNDSFKGKTVVISG
HHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHCCCCCCCEEEEEC
AGNVAIYAAQKAEELGAKVVTVSDSNGYVYDPNGIDVAVVKQIKEVERGRIKEYAERVTS
CCCEEEEEEHHHHHCCCEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
AEYHEGCSGVWTVPCDIALPCATQNEIDEESAKALVANGCKVVCEGANMPSTPEAIEVYK
HHHHCCCCCEEEECCCEEECCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHH
SNKLLYGPAKAANAGGVAVSGLEMSQNSYRLSWTFEEVDAKLKSIMESIVANSLAAAKEY
CCEEEECCCCCCCCCCEEEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHC
GEEGDLMAGANIAGFVKVANAMVAQGVL
CCCCCEEECCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]