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The map label for this gene is maiA [H]

Identifier: 213159157

GI number: 213159157

Start: 3994377

End: 3994913

Strand: Direct

Name: maiA [H]

Synonym: AB57_3868

Alternate gene names: 213159157

Gene position: 3994377-3994913 (Clockwise)

Preceding gene: 213159156

Following gene: 213159158

Centisome position: 98.61

GC content: 39.11

Gene sequence:

>537_bases
TTGGTTAAAAATGAGCAGCAAAGTGAAGACTATTTAAAGTTAAATCGCAGTGCCTTAGTACCAACTTTCGTGGACGGCGA
TTTAACCCTGTCACAGTCATTAAGTATTTTGGAATATCTGGATGAACAATATCCTGAAACCAAATTACTTCCAAATGATG
TGAAAGAAAGAGCCAAAATCCGTGCTTTTGCACAGGCGATTGCCTGTGATATTCACCCGTTAAATAACTTACGCGTGCTG
AAATATTTAAAAAATGATTTAAATGTTTCGGACGAACAAAAAAATTACTGGTATCAGCACTGGATTCTTGAAGGCTTTCA
AACACTCGAACAGCAACTTCAAGATTCAAATGGACAATTTTGCTTTGGACAACACGCAACCATTGCCGACTGCTGCCTGA
TTCCACAGGTTTACAACGCGAAACGCTTCAAGATTGATTTGTCGGCGTTTCCAAAAATTGAATCAATTTATCAACATTGT
TTGTCTATCCCAGCCTTCTATAACGCGGCGCCAGAACAACAACCAGATTGGGAATAG

Upstream 100 bases:

>100_bases
AATTATGAAGCTGTATAGTTACTTTCGTAGCTCTGCAGCTTACAGAGTACGGATTGGTCTCAACCTTAAAAGTCTCGCTT
ATGAAACTGTACCCGTTCAT

Downstream 100 bases:

>100_bases
TAAAAAGGAGTTTCAACATGACATTTGCCATTAAAAAAATACATCACGTTGCATATCGCTGTAAGGATGCGAAAGAAACT
GTTGAGTGGTATAAAAAAAT

Product: maleylacetoacetate isomerase

Products: NA

Alternate protein names: MAAI [H]

Number of amino acids: Translated: 178; Mature: 178

Protein sequence:

>178_residues
MVKNEQQSEDYLKLNRSALVPTFVDGDLTLSQSLSILEYLDEQYPETKLLPNDVKERAKIRAFAQAIACDIHPLNNLRVL
KYLKNDLNVSDEQKNYWYQHWILEGFQTLEQQLQDSNGQFCFGQHATIADCCLIPQVYNAKRFKIDLSAFPKIESIYQHC
LSIPAFYNAAPEQQPDWE

Sequences:

>Translated_178_residues
MVKNEQQSEDYLKLNRSALVPTFVDGDLTLSQSLSILEYLDEQYPETKLLPNDVKERAKIRAFAQAIACDIHPLNNLRVL
KYLKNDLNVSDEQKNYWYQHWILEGFQTLEQQLQDSNGQFCFGQHATIADCCLIPQVYNAKRFKIDLSAFPKIESIYQHC
LSIPAFYNAAPEQQPDWE
>Mature_178_residues
MVKNEQQSEDYLKLNRSALVPTFVDGDLTLSQSLSILEYLDEQYPETKLLPNDVKERAKIRAFAQAIACDIHPLNNLRVL
KYLKNDLNVSDEQKNYWYQHWILEGFQTLEQQLQDSNGQFCFGQHATIADCCLIPQVYNAKRFKIDLSAFPKIESIYQHC
LSIPAFYNAAPEQQPDWE

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI22202624, Length=173, Percent_Identity=44.5086705202312, Blast_Score=145, Evalue=2e-35,
Organism=Homo sapiens, GI22202622, Length=157, Percent_Identity=45.859872611465, Blast_Score=140, Evalue=6e-34,
Organism=Homo sapiens, GI194440732, Length=102, Percent_Identity=45.0980392156863, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17551302, Length=174, Percent_Identity=36.7816091954023, Blast_Score=123, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17556142, Length=174, Percent_Identity=37.3563218390805, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17510461, Length=171, Percent_Identity=35.672514619883, Blast_Score=114, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24645375, Length=174, Percent_Identity=43.1034482758621, Blast_Score=148, Evalue=1e-36,
Organism=Drosophila melanogaster, GI45553325, Length=174, Percent_Identity=43.1034482758621, Blast_Score=148, Evalue=2e-36,
Organism=Drosophila melanogaster, GI21355859, Length=174, Percent_Identity=43.1034482758621, Blast_Score=148, Evalue=2e-36,
Organism=Drosophila melanogaster, GI21355857, Length=176, Percent_Identity=39.7727272727273, Blast_Score=131, Evalue=3e-31,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR005955
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: =5.2.1.2 [H]

Molecular weight: Translated: 20691; Mature: 20691

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKNEQQSEDYLKLNRSALVPTFVDGDLTLSQSLSILEYLDEQYPETKLLPNDVKERAKI
CCCCCCCCHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
RAFAQAIACDIHPLNNLRVLKYLKNDLNVSDEQKNYWYQHWILEGFQTLEQQLQDSNGQF
HHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCE
CFGQHATIADCCLIPQVYNAKRFKIDLSAFPKIESIYQHCLSIPAFYNAAPEQQPDWE
EECCCCHHHHHHHHHHHCCCEEEEEEHHHCCCHHHHHHHHHCCCHHHCCCCCCCCCCC
>Mature Secondary Structure
MVKNEQQSEDYLKLNRSALVPTFVDGDLTLSQSLSILEYLDEQYPETKLLPNDVKERAKI
CCCCCCCCHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
RAFAQAIACDIHPLNNLRVLKYLKNDLNVSDEQKNYWYQHWILEGFQTLEQQLQDSNGQF
HHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCE
CFGQHATIADCCLIPQVYNAKRFKIDLSAFPKIESIYQHCLSIPAFYNAAPEQQPDWE
EECCCCHHHHHHHHHHHCCCEEEEEEHHHCCCHHHHHHHHHCCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]