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The map label for this gene is gshA [H]

Identifier: 213159109

GI number: 213159109

Start: 3940199

End: 3941776

Strand: Reverse

Name: gshA [H]

Synonym: AB57_3819

Alternate gene names: 213159109

Gene position: 3941776-3940199 (Counterclockwise)

Preceding gene: 213159115

Following gene: 213159108

Centisome position: 97.32

GC content: 41.19

Gene sequence:

>1578_bases
ATGAGTCAACCCACTACACCATCTCAATCAGTTATTCCTGCTTGGGTGGATTCGTCGCTACTACAAGGCATGTTACGTGG
AATAGAACGTGAAAGCCTACGTATGCAAAGTAATGGGTTCTTATCACAAGAGTTACATCCAAAGGCATTAGGTTCAGCTT
TAACACATCCGAAAATCACCACAGATTATTCTGAAGCGTTGATGGAGTTTATTACTTCACCACAGCCAACCATTGGTGAT
GCCTTGCATGAGCTCACCGATATTCATGCGGTTGTGCACCGTCATTTAGAAAATGGTGAAAAGCTCTGGCCGTTGTCTAT
GCCATGTATGCTAGATGACAATGAAGAACGTATTCGTCTAGCTCAATATGGCACTTCTAATATTGGCCGTTTCAAGACGC
TTTATCGCCGTGGTTTAGGTATTCGTTATGGTCGCCGTATGCAGACGATTTCGGGCGTACACTATAATTTATCTTTCCCT
GATTCTCTTTTTTCTGCCTTGCAGGCACATGAAACTGACGAAAAATTAAAAGCACTTAGTCTGCAAGATTATCGTAGTCA
TCGCTATTTTGGTCTGATTCGTAACTTTATTCGTTTAACGCCACTGGTTATGTTTTTAGTGGGAGCGAGCCCATCGGTTT
GCCGTTGTTTTCTTACGGGTCATGAACATCATTTGTTGCCTTTAATCAAAGGGTCATATTATTTACCGTATGCTACAGCC
CTACGTATGGGACGTTTAGGCTACCAAAACTCGGCACAAAAAAGTTTAGGCATTCACTATAACAACTTAACTGGTTATTT
GGATGGTTTACAAAAAGCAGTACATTCTCCATACCCGCCATTTAGCCGTTTAGGGTTAAATGATGCCTCTGGTGAACCAT
TGCAAATTAATGATCATGTTTTACAAATCGAGAATGAATATTACAGTCTCGTGCGTCCAAAACAAGTTCCTCAAGCGGGT
GAAACACCGTCTCAGGCCTTGAAAAATCGAGGAGTGGGTTATGTTGAACTTCGTGCAGTGGACGTAAATCCATATTCGGC
AATTGGCGTCAATGAAACAACAGCAGGTTTCCTTGAGGCATTAGCGCTTTATTGTTTATTAAGCGATAGTCCTGAACTGT
TATGCCCTGAGCAAGATTTGATTGAGAAAAACCAGACAGAAGTGGTTAATCGTGGTCGAGCACCAAACGCAACCATTACT
GATTTAAACGGGTCTTACCATATTGAAGATTGGGCACGTCAGCATATTTCTAAAATGCAGGATTGTGCTCGCTTACTCGA
TCAGACCTATGCAACAGATCTATACAGCTCGGCTTTGGCTGTGATGCAAGAACGTATTGATGAAGTAGATGAAACATTAT
CTGCTCATGTGATTGATGACACATTAAAGCATGGTGGAACCTGGAGCTTTGGTAGTCATATGGCACAGTTACATGCTGAA
ACTTATGAAAAGCATGAGATTAGTGCAGAGACTTTGCAATATTTTGAACAATTGGCTGTGCAATCTTTACAGCAGCAAGA
ACAACTTGAGCAAGATAGTCAGATCAGTTTTGATCAATATCTTGAACAATATAGATAA

Upstream 100 bases:

>100_bases
ATTGTGATAAAAATTGCCAAACTGAAATGATGTGATCATATCAATAGCCGTATCATGTCACGATTTTGTATATAGTGATC
TTTTATAAGCGAGCAAATGC

Downstream 100 bases:

>100_bases
AAACGAGGTTTTACATGCGATTAAGTTACCGTTTGGTGAGTGGACTGCTTGTTCTGGCAAGCATTGTCGGCATGTCTTTT
GCGTTGTATTTGGAACATGT

Product: glutamate--cysteine ligase

Products: NA

Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase [H]

Number of amino acids: Translated: 525; Mature: 524

Protein sequence:

>525_residues
MSQPTTPSQSVIPAWVDSSLLQGMLRGIERESLRMQSNGFLSQELHPKALGSALTHPKITTDYSEALMEFITSPQPTIGD
ALHELTDIHAVVHRHLENGEKLWPLSMPCMLDDNEERIRLAQYGTSNIGRFKTLYRRGLGIRYGRRMQTISGVHYNLSFP
DSLFSALQAHETDEKLKALSLQDYRSHRYFGLIRNFIRLTPLVMFLVGASPSVCRCFLTGHEHHLLPLIKGSYYLPYATA
LRMGRLGYQNSAQKSLGIHYNNLTGYLDGLQKAVHSPYPPFSRLGLNDASGEPLQINDHVLQIENEYYSLVRPKQVPQAG
ETPSQALKNRGVGYVELRAVDVNPYSAIGVNETTAGFLEALALYCLLSDSPELLCPEQDLIEKNQTEVVNRGRAPNATIT
DLNGSYHIEDWARQHISKMQDCARLLDQTYATDLYSSALAVMQERIDEVDETLSAHVIDDTLKHGGTWSFGSHMAQLHAE
TYEKHEISAETLQYFEQLAVQSLQQQEQLEQDSQISFDQYLEQYR

Sequences:

>Translated_525_residues
MSQPTTPSQSVIPAWVDSSLLQGMLRGIERESLRMQSNGFLSQELHPKALGSALTHPKITTDYSEALMEFITSPQPTIGD
ALHELTDIHAVVHRHLENGEKLWPLSMPCMLDDNEERIRLAQYGTSNIGRFKTLYRRGLGIRYGRRMQTISGVHYNLSFP
DSLFSALQAHETDEKLKALSLQDYRSHRYFGLIRNFIRLTPLVMFLVGASPSVCRCFLTGHEHHLLPLIKGSYYLPYATA
LRMGRLGYQNSAQKSLGIHYNNLTGYLDGLQKAVHSPYPPFSRLGLNDASGEPLQINDHVLQIENEYYSLVRPKQVPQAG
ETPSQALKNRGVGYVELRAVDVNPYSAIGVNETTAGFLEALALYCLLSDSPELLCPEQDLIEKNQTEVVNRGRAPNATIT
DLNGSYHIEDWARQHISKMQDCARLLDQTYATDLYSSALAVMQERIDEVDETLSAHVIDDTLKHGGTWSFGSHMAQLHAE
TYEKHEISAETLQYFEQLAVQSLQQQEQLEQDSQISFDQYLEQYR
>Mature_524_residues
SQPTTPSQSVIPAWVDSSLLQGMLRGIERESLRMQSNGFLSQELHPKALGSALTHPKITTDYSEALMEFITSPQPTIGDA
LHELTDIHAVVHRHLENGEKLWPLSMPCMLDDNEERIRLAQYGTSNIGRFKTLYRRGLGIRYGRRMQTISGVHYNLSFPD
SLFSALQAHETDEKLKALSLQDYRSHRYFGLIRNFIRLTPLVMFLVGASPSVCRCFLTGHEHHLLPLIKGSYYLPYATAL
RMGRLGYQNSAQKSLGIHYNNLTGYLDGLQKAVHSPYPPFSRLGLNDASGEPLQINDHVLQIENEYYSLVRPKQVPQAGE
TPSQALKNRGVGYVELRAVDVNPYSAIGVNETTAGFLEALALYCLLSDSPELLCPEQDLIEKNQTEVVNRGRAPNATITD
LNGSYHIEDWARQHISKMQDCARLLDQTYATDLYSSALAVMQERIDEVDETLSAHVIDDTLKHGGTWSFGSHMAQLHAET
YEKHEISAETLQYFEQLAVQSLQQQEQLEQDSQISFDQYLEQYR

Specific function: Glutathione biosynthesis; first step. [C]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789044, Length=534, Percent_Identity=38.5767790262172, Blast_Score=350, Evalue=2e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007370
- InterPro:   IPR006334 [H]

Pfam domain/function: PF04262 Glu_cys_ligase [H]

EC number: =6.3.2.2 [H]

Molecular weight: Translated: 59316; Mature: 59184

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPTTPSQSVIPAWVDSSLLQGMLRGIERESLRMQSNGFLSQELHPKALGSALTHPKIT
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCC
TDYSEALMEFITSPQPTIGDALHELTDIHAVVHRHLENGEKLWPLSMPCMLDDNEERIRL
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCEECCCHHHEEH
AQYGTSNIGRFKTLYRRGLGIRYGRRMQTISGVHYNLSFPDSLFSALQAHETDEKLKALS
HHCCCCCHHHHHHHHHHCCCCHHCCHHHHHCCCEEECCCCHHHHHHHHHHCHHHHHHHHH
LQDYRSHRYFGLIRNFIRLTPLVMFLVGASPSVCRCFLTGHEHHLLPLIKGSYYLPYATA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHEECCCCCCEEHEECCCCCCHHHHH
LRMGRLGYQNSAQKSLGIHYNNLTGYLDGLQKAVHSPYPPFSRLGLNDASGEPLQINDHV
HHHHHCCCCCCCHHHCCCEECCHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCEEECCCE
LQIENEYYSLVRPKQVPQAGETPSQALKNRGVGYVELRAVDVNPYSAIGVNETTAGFLEA
EEECHHHHHHCCCCCCCCCCCCHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHH
LALYCLLSDSPELLCPEQDLIEKNQTEVVNRGRAPNATITDLNGSYHIEDWARQHISKMQ
HHHHHHHCCCCCCCCCCHHHHHCHHHHHHHCCCCCCCEEEECCCCEEHHHHHHHHHHHHH
DCARLLDQTYATDLYSSALAVMQERIDEVDETLSAHVIDDTLKHGGTWSFGSHMAQLHAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
TYEKHEISAETLQYFEQLAVQSLQQQEQLEQDSQISFDQYLEQYR
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCC
>Mature Secondary Structure 
SQPTTPSQSVIPAWVDSSLLQGMLRGIERESLRMQSNGFLSQELHPKALGSALTHPKIT
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCC
TDYSEALMEFITSPQPTIGDALHELTDIHAVVHRHLENGEKLWPLSMPCMLDDNEERIRL
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCEECCCHHHEEH
AQYGTSNIGRFKTLYRRGLGIRYGRRMQTISGVHYNLSFPDSLFSALQAHETDEKLKALS
HHCCCCCHHHHHHHHHHCCCCHHCCHHHHHCCCEEECCCCHHHHHHHHHHCHHHHHHHHH
LQDYRSHRYFGLIRNFIRLTPLVMFLVGASPSVCRCFLTGHEHHLLPLIKGSYYLPYATA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHEECCCCCCEEHEECCCCCCHHHHH
LRMGRLGYQNSAQKSLGIHYNNLTGYLDGLQKAVHSPYPPFSRLGLNDASGEPLQINDHV
HHHHHCCCCCCCHHHCCCEECCHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCEEECCCE
LQIENEYYSLVRPKQVPQAGETPSQALKNRGVGYVELRAVDVNPYSAIGVNETTAGFLEA
EEECHHHHHHCCCCCCCCCCCCHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHH
LALYCLLSDSPELLCPEQDLIEKNQTEVVNRGRAPNATITDLNGSYHIEDWARQHISKMQ
HHHHHHHCCCCCCCCCCHHHHHCHHHHHHHCCCCCCCEEEECCCCEEHHHHHHHHHHHHH
DCARLLDQTYATDLYSSALAVMQERIDEVDETLSAHVIDDTLKHGGTWSFGSHMAQLHAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
TYEKHEISAETLQYFEQLAVQSLQQQEQLEQDSQISFDQYLEQYR
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA