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The map label for this gene is est [H]

Identifier: 213159085

GI number: 213159085

Start: 3914453

End: 3915364

Strand: Direct

Name: est [H]

Synonym: AB57_3795

Alternate gene names: 213159085

Gene position: 3914453-3915364 (Clockwise)

Preceding gene: 213159083

Following gene: 213159087

Centisome position: 96.64

GC content: 39.14

Gene sequence:

>912_bases
ATGAATATACCTAATGTTTTAAAATATTATTTCACAGAAACATTTCTTAAAACAGCAATTCGCAAACCAAGTCAGTTGAA
TTTACCTCCAACTGCTCTGCGCCCTATGCTTGAACAACTTTGCCGAGCCTTTCCAAAACAAAAAAATGTAACCGTTCGAC
CAATCCGACTTGCTGGCATTAAGGGTGAAGAAATTAAAGCCCAAGATTCAGCGACCCAGCTTATTTTTCATATTCACGGC
GGCGCCTTTTTTTTAGGAAGTTTAAAAACACATCATGCGTTCATGACAGATTTGGCTGCTCGTACCCAAATGCAAATTAT
TCATGTGGACTATCCACTTGCACCAGAGCATCCATATCCTGAGGCGACTGAGGCCTTATATGACGTCTATCAATCATTAT
TAGTTCAAGGTATCCAGCCTAAAGATATTATTTTATCTGGTGACTCTTGTGGAGCTAATTTGGCTTTGGCACTTTGCTTA
CGTTTAAAAGAACAACCCGAACTTATGCCAAGTGGCCTGATTTTATTGTCACCTTTTCTAGATTTAACCCTAACCAGTGA
GTCTTTAAGATTTAACCAAAAACATGATGCCCTACTTTCTCTTGAAGCTCTTCAGATCGGTATTCAACATTATATTGGCA
ATCATATTGCGGCAGATGACCCGCGTGTTTCACCTATTTTCGATAACTTAGAAGGTTTACCGCCAACATTAGTTCAGGTC
GGTTCAAAAGAAATTTTATTAGACGATGCCAAACGTTTTAGAGAGAAAGCTGAAGAAGCAGGTGTCAAAGTACACTTTAA
ACTTTACACAGGCATGTGGCATAACTTCCAAATGTTTAATGCTTGGTTCGAAGAAGCAAAACAGGCGATGGCGGATATTG
CTGAGTTTGCTCATGAACTTGATAAGACGTAA

Upstream 100 bases:

>100_bases
ACGATATTGCAATTTTTGAATGTGACTGTTTCGTGTAACCTTGTAACCTTGAAAAATAAAATTTATAGGGAAACAAGTAA
TTAACATAAGCGGATACTAT

Downstream 100 bases:

>100_bases
TCAAAATGAAGAAGTGACAAATTTAAAATTTGTCACTTCTTCTTATTTTTCACAATCCAAATCATGAGGTTTAATTTATG
AATCATGGTATGTTTAGGTT

Product: esterase

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MNIPNVLKYYFTETFLKTAIRKPSQLNLPPTALRPMLEQLCRAFPKQKNVTVRPIRLAGIKGEEIKAQDSATQLIFHIHG
GAFFLGSLKTHHAFMTDLAARTQMQIIHVDYPLAPEHPYPEATEALYDVYQSLLVQGIQPKDIILSGDSCGANLALALCL
RLKEQPELMPSGLILLSPFLDLTLTSESLRFNQKHDALLSLEALQIGIQHYIGNHIAADDPRVSPIFDNLEGLPPTLVQV
GSKEILLDDAKRFREKAEEAGVKVHFKLYTGMWHNFQMFNAWFEEAKQAMADIAEFAHELDKT

Sequences:

>Translated_303_residues
MNIPNVLKYYFTETFLKTAIRKPSQLNLPPTALRPMLEQLCRAFPKQKNVTVRPIRLAGIKGEEIKAQDSATQLIFHIHG
GAFFLGSLKTHHAFMTDLAARTQMQIIHVDYPLAPEHPYPEATEALYDVYQSLLVQGIQPKDIILSGDSCGANLALALCL
RLKEQPELMPSGLILLSPFLDLTLTSESLRFNQKHDALLSLEALQIGIQHYIGNHIAADDPRVSPIFDNLEGLPPTLVQV
GSKEILLDDAKRFREKAEEAGVKVHFKLYTGMWHNFQMFNAWFEEAKQAMADIAEFAHELDKT
>Mature_303_residues
MNIPNVLKYYFTETFLKTAIRKPSQLNLPPTALRPMLEQLCRAFPKQKNVTVRPIRLAGIKGEEIKAQDSATQLIFHIHG
GAFFLGSLKTHHAFMTDLAARTQMQIIHVDYPLAPEHPYPEATEALYDVYQSLLVQGIQPKDIILSGDSCGANLALALCL
RLKEQPELMPSGLILLSPFLDLTLTSESLRFNQKHDALLSLEALQIGIQHYIGNHIAADDPRVSPIFDNLEGLPPTLVQV
GSKEILLDDAKRFREKAEEAGVKVHFKLYTGMWHNFQMFNAWFEEAKQAMADIAEFAHELDKT

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Secreted (Potential). Note=External to the cytoplasmic membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21328446, Length=144, Percent_Identity=31.25, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1786682, Length=254, Percent_Identity=28.740157480315, Blast_Score=89, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17567059, Length=315, Percent_Identity=26.6666666666667, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI115533410, Length=113, Percent_Identity=32.7433628318584, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI115533412, Length=113, Percent_Identity=32.7433628318584, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24656084, Length=106, Percent_Identity=38.6792452830189, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24656076, Length=106, Percent_Identity=38.6792452830189, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI20130169, Length=106, Percent_Identity=38.6792452830189, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 34113; Mature: 34113

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01173 LIPASE_GDXG_HIS ; PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIPNVLKYYFTETFLKTAIRKPSQLNLPPTALRPMLEQLCRAFPKQKNVTVRPIRLAGI
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECC
KGEEIKAQDSATQLIFHIHGGAFFLGSLKTHHAFMTDLAARTQMQIIHVDYPLAPEHPYP
CCCCCCCCCCCEEEEEEECCCEEEECCHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCC
EATEALYDVYQSLLVQGIQPKDIILSGDSCGANLALALCLRLKEQPELMPSGLILLSPFL
HHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
DLTLTSESLRFNQKHDALLSLEALQIGIQHYIGNHIAADDPRVSPIFDNLEGLPPTLVQV
HHEECHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCHHHHHC
GSKEILLDDAKRFREKAEEAGVKVHFKLYTGMWHNFQMFNAWFEEAKQAMADIAEFAHEL
CCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DKT
CCC
>Mature Secondary Structure
MNIPNVLKYYFTETFLKTAIRKPSQLNLPPTALRPMLEQLCRAFPKQKNVTVRPIRLAGI
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECC
KGEEIKAQDSATQLIFHIHGGAFFLGSLKTHHAFMTDLAARTQMQIIHVDYPLAPEHPYP
CCCCCCCCCCCEEEEEEECCCEEEECCHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCC
EATEALYDVYQSLLVQGIQPKDIILSGDSCGANLALALCLRLKEQPELMPSGLILLSPFL
HHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
DLTLTSESLRFNQKHDALLSLEALQIGIQHYIGNHIAADDPRVSPIFDNLEGLPPTLVQV
HHEECHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCHHHHHC
GSKEILLDDAKRFREKAEEAGVKVHFKLYTGMWHNFQMFNAWFEEAKQAMADIAEFAHEL
CCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DKT
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2545531 [H]