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The map label for this gene is prfB [H]

Identifier: 213159055

GI number: 213159055

Start: 3880439

End: 3881371

Strand: Reverse

Name: prfB [H]

Synonym: AB57_3765

Alternate gene names: 213159055

Gene position: 3881371-3880439 (Counterclockwise)

Preceding gene: 213159057

Following gene: 213159054

Centisome position: 95.82

GC content: 41.69

Gene sequence:

>933_bases
ATGGCGAAAGAAAAGGGCGAACTAGAAAACGTTATTAATGTATTAGATGGCTTATCTACACAGCTTGAAGATGCAAAAGC
AATGTTGGATTTGGCTGTAGAAGCTGATGATGAAAGCTTACTTGAAGATGTTCAGTCTGAACTGAGTACTGCTGAAGAAG
AATTAGCAAAACTTGAATTCCGCCGTATGTTTAGCAATCCAATGGACCCGAATCCTTGCTATGTCGAGATTCAGGCGGGT
TCAGGTGGTACAGAAGCACAAGATTGGGCTTCAATGCTATTACGTATGTATATGCGCTGGATTGAACGTCATGGTTTTAA
AGCAGAACTTATGGAAGTCTCTGACGGTGACGTCGCAGGTATCAAATCTGCAACGATTCGTGTTGAAGGTGAATATGCGT
ATGGTTGGTTACGTACAGAATCTGGTGTACACCGTTTAGTACGTAAATCACCATTTGACAGTGGTAACCGTCGTCATACT
TCTTTCTCTGCGGTATTTATTTCTCCAGAAGTCGATGACAATATCGAAATTGATATTAATCCTTCAGATGTTCGTACTGA
TACTTACCGTGCATCTGGTGCAGGTGGTCAGCACATTAACAAAACCGATTCGGCAGTACGTTTGACGCATATTCCGACTG
GTATTGTAGTGGCATGTCAGAACCAGCGTTCACAGCATGCGAACCGTGATCATGCATGGAAACAGTTACGTGCAAAATTG
TATGAATTAGAAATGCAAAAACGTAATGAAGCCGCTCAAGCACTTGAAGACTCGAAGTCTGATATTGGTTGGGGTAGCCA
AATTCGTTCTTATGTACTTGATGACTCTCGAATCAAGGATTTACGTACGGGTGTTGAAAATTCAAATACTGGTGCGGTTC
TTGATGGTGACCTTGATCGTTTTATTGAAGCGAGTTTAAAACAGGGGTTATAA

Upstream 100 bases:

>100_bases
GGGTATCTTTGACTACGATCTAAAAAAAGAACGTTTAGAAGAAGTTTTACGTGAGTTAGAAGACCCTGCGATCTGGAATG
ATCAAAACCGTGCTCAGGCA

Downstream 100 bases:

>100_bases
GAGTCTTTTGAACTTTTAATTCTCTATAACTCTTAAATTTTTAGCGATTAAAACGGCAAAGCAGTTCAGTGTTTGGAAGT
TAATCGGAAAAATACGATAT

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MAKEKGELENVINVLDGLSTQLEDAKAMLDLAVEADDESLLEDVQSELSTAEEELAKLEFRRMFSNPMDPNPCYVEIQAG
SGGTEAQDWASMLLRMYMRWIERHGFKAELMEVSDGDVAGIKSATIRVEGEYAYGWLRTESGVHRLVRKSPFDSGNRRHT
SFSAVFISPEVDDNIEIDINPSDVRTDTYRASGAGGQHINKTDSAVRLTHIPTGIVVACQNQRSQHANRDHAWKQLRAKL
YELEMQKRNEAAQALEDSKSDIGWGSQIRSYVLDDSRIKDLRTGVENSNTGAVLDGDLDRFIEASLKQGL

Sequences:

>Translated_310_residues
MAKEKGELENVINVLDGLSTQLEDAKAMLDLAVEADDESLLEDVQSELSTAEEELAKLEFRRMFSNPMDPNPCYVEIQAG
SGGTEAQDWASMLLRMYMRWIERHGFKAELMEVSDGDVAGIKSATIRVEGEYAYGWLRTESGVHRLVRKSPFDSGNRRHT
SFSAVFISPEVDDNIEIDINPSDVRTDTYRASGAGGQHINKTDSAVRLTHIPTGIVVACQNQRSQHANRDHAWKQLRAKL
YELEMQKRNEAAQALEDSKSDIGWGSQIRSYVLDDSRIKDLRTGVENSNTGAVLDGDLDRFIEASLKQGL
>Mature_309_residues
AKEKGELENVINVLDGLSTQLEDAKAMLDLAVEADDESLLEDVQSELSTAEEELAKLEFRRMFSNPMDPNPCYVEIQAGS
GGTEAQDWASMLLRMYMRWIERHGFKAELMEVSDGDVAGIKSATIRVEGEYAYGWLRTESGVHRLVRKSPFDSGNRRHTS
FSAVFISPEVDDNIEIDINPSDVRTDTYRASGAGGQHINKTDSAVRLTHIPTGIVVACQNQRSQHANRDHAWKQLRAKLY
ELEMQKRNEAAQALEDSKSDIGWGSQIRSYVLDDSRIKDLRTGVENSNTGAVLDGDLDRFIEASLKQGL

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=314, Percent_Identity=34.0764331210191, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI34577120, Length=301, Percent_Identity=32.5581395348837, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI166795305, Length=213, Percent_Identity=33.8028169014084, Blast_Score=118, Evalue=9e-27,
Organism=Escherichia coli, GI2367172, Length=310, Percent_Identity=69.3548387096774, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI1787462, Length=294, Percent_Identity=38.0952380952381, Blast_Score=191, Evalue=7e-50,
Organism=Caenorhabditis elegans, GI17542784, Length=311, Percent_Identity=34.4051446945338, Blast_Score=131, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6321295, Length=298, Percent_Identity=34.5637583892617, Blast_Score=151, Evalue=1e-37,
Organism=Drosophila melanogaster, GI19921226, Length=224, Percent_Identity=37.0535714285714, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 34638; Mature: 34507

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKGELENVINVLDGLSTQLEDAKAMLDLAVEADDESLLEDVQSELSTAEEELAKLEF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
RRMFSNPMDPNPCYVEIQAGSGGTEAQDWASMLLRMYMRWIERHGFKAELMEVSDGDVAG
HHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
IKSATIRVEGEYAYGWLRTESGVHRLVRKSPFDSGNRRHTSFSAVFISPEVDDNIEIDIN
CCEEEEEEECCEEEEEEECHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEC
PSDVRTDTYRASGAGGQHINKTDSAVRLTHIPTGIVVACQNQRSQHANRDHAWKQLRAKL
CCCCCCCCEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCCHHHCCCHHHHHHHHHHHH
YELEMQKRNEAAQALEDSKSDIGWGSQIRSYVLDDSRIKDLRTGVENSNTGAVLDGDLDR
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEECCHHHH
FIEASLKQGL
HHHHHHHHCC
>Mature Secondary Structure 
AKEKGELENVINVLDGLSTQLEDAKAMLDLAVEADDESLLEDVQSELSTAEEELAKLEF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
RRMFSNPMDPNPCYVEIQAGSGGTEAQDWASMLLRMYMRWIERHGFKAELMEVSDGDVAG
HHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
IKSATIRVEGEYAYGWLRTESGVHRLVRKSPFDSGNRRHTSFSAVFISPEVDDNIEIDIN
CCEEEEEEECCEEEEEEECHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEC
PSDVRTDTYRASGAGGQHINKTDSAVRLTHIPTGIVVACQNQRSQHANRDHAWKQLRAKL
CCCCCCCCEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCCHHHCCCHHHHHHHHHHHH
YELEMQKRNEAAQALEDSKSDIGWGSQIRSYVLDDSRIKDLRTGVENSNTGAVLDGDLDR
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEECCHHHH
FIEASLKQGL
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA