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The map label for this gene is nemR [H]

Identifier: 213159033

GI number: 213159033

Start: 3851333

End: 3851935

Strand: Reverse

Name: nemR [H]

Synonym: AB57_3741

Alternate gene names: 213159033

Gene position: 3851935-3851333 (Counterclockwise)

Preceding gene: 213159035

Following gene: 213159032

Centisome position: 95.1

GC content: 41.96

Gene sequence:

>603_bases
ATGAAACGATTAAATAAAAGCGAAACCACACGACAACACATTTTAGATACCAGTTTTGAACTGGTGCTACATAAAGGTTT
TGTCGGTGTTGGTTTGCAAGAAATTTTAAAGGCTTGTGACGTACCTAAAGGTTCGTTCTATCACTACTTTGCTTCAAAAG
AGGCGTTTGGCTGTGCGTTGCTCGAGCAATACATGGCCAATTATAAAGTCCGTATGGAACAGCTTTGGCAGCACAGTGAG
CAAAGTGCCCATGCCCGTTTAATGGCTTTATGGCAGGCGTGGATTGATGATCCGGTCCATGGTAGCTGGGCTGAAAACTG
CTTGATTGTGAAATTGGCAGCCGAAGTTTCGGACTTATCAGAAGATATGCGTCAAATTCTAAATGACGGTGTGCACAAAT
TAACGCAGCGTCTAGCTTTGCTACTCAAAGAAGGGCAACAAGAAGGATCAATTCCAAAACATATTGAACCTTTGAAAACT
GCGCAAGTCATGTATCAGTTATGGTTAGGTGCTGCTTTACTCACAAAATTGTCGCAAGACAAAGCACATCTACATCTAGC
CTTAGAAACCACCCAACAACTCTTAAAACCGATAGAGGAATAA

Upstream 100 bases:

>100_bases
TTTTTAACATTCCTCATTCTACATTTCTACTCTAAGCAAATTTTAACCAGCAACATAATTTTACTAGACGACTGGTCTAA
TAGTTGTTTATGATGAATGC

Downstream 100 bases:

>100_bases
AGCATGCGAAGTATTATCCATCGTAATTTTGGCGAACCTGTAGACGTTTTAGAGCAGGCGGATATGCCAAAGCCTGAACC
AAAAGCAGGCGAAGTGCGTA

Product: transcriptional regulator, TetR family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MKRLNKSETTRQHILDTSFELVLHKGFVGVGLQEILKACDVPKGSFYHYFASKEAFGCALLEQYMANYKVRMEQLWQHSE
QSAHARLMALWQAWIDDPVHGSWAENCLIVKLAAEVSDLSEDMRQILNDGVHKLTQRLALLLKEGQQEGSIPKHIEPLKT
AQVMYQLWLGAALLTKLSQDKAHLHLALETTQQLLKPIEE

Sequences:

>Translated_200_residues
MKRLNKSETTRQHILDTSFELVLHKGFVGVGLQEILKACDVPKGSFYHYFASKEAFGCALLEQYMANYKVRMEQLWQHSE
QSAHARLMALWQAWIDDPVHGSWAENCLIVKLAAEVSDLSEDMRQILNDGVHKLTQRLALLLKEGQQEGSIPKHIEPLKT
AQVMYQLWLGAALLTKLSQDKAHLHLALETTQQLLKPIEE
>Mature_200_residues
MKRLNKSETTRQHILDTSFELVLHKGFVGVGLQEILKACDVPKGSFYHYFASKEAFGCALLEQYMANYKVRMEQLWQHSE
QSAHARLMALWQAWIDDPVHGSWAENCLIVKLAAEVSDLSEDMRQILNDGVHKLTQRLALLLKEGQQEGSIPKHIEPLKT
AQVMYQLWLGAALLTKLSQDKAHLHLALETTQQLLKPIEE

Specific function: Represses the transcription of the nemRA operon by binding to the nemR box [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI145693141, Length=186, Percent_Identity=43.010752688172, Blast_Score=154, Evalue=6e-39,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR001647 [H]

Pfam domain/function: PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 22829; Mature: 22829

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLNKSETTRQHILDTSFELVLHKGFVGVGLQEILKACDVPKGSFYHYFASKEAFGCAL
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHCCHHHHHHH
LEQYMANYKVRMEQLWQHSEQSAHARLMALWQAWIDDPVHGSWAENCLIVKLAAEVSDLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCEEEEHHHHHHHHHH
EDMRQILNDGVHKLTQRLALLLKEGQQEGSIPKHIEPLKTAQVMYQLWLGAALLTKLSQD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
KAHLHLALETTQQLLKPIEE
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRLNKSETTRQHILDTSFELVLHKGFVGVGLQEILKACDVPKGSFYHYFASKEAFGCAL
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHCCHHHHHHH
LEQYMANYKVRMEQLWQHSEQSAHARLMALWQAWIDDPVHGSWAENCLIVKLAAEVSDLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCEEEEHHHHHHHHHH
EDMRQILNDGVHKLTQRLALLLKEGQQEGSIPKHIEPLKTAQVMYQLWLGAALLTKLSQD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
KAHLHLALETTQQLLKPIEE
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]