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The map label for this gene is gdhA [H]

Identifier: 213158889

GI number: 213158889

Start: 3695272

End: 3696543

Strand: Reverse

Name: gdhA [H]

Synonym: AB57_3588

Alternate gene names: 213158889

Gene position: 3696543-3695272 (Counterclockwise)

Preceding gene: 213158890

Following gene: 213158888

Centisome position: 91.26

GC content: 43.0

Gene sequence:

>1272_bases
ATGTCAGATCTTTCATACGTAAACGAACATAGCGATGCATGGCAAACTTACTTGGCTCAAATTGACCGTGTAGCGCCATA
CTTAGAACATTTAGCTGATTATATCGATACTTTAAAACGTCCTAAGCGTGCGCTTATTGTGGACGTGCCAATTGTAATGG
ACGACGGTTCAATCCAGCACTTTGAAGGCTACCGTGTACAACACAACTTGTCTCGTGGTCCTGGTAAAGGTGGTGTTCGT
TACCATCCAAATGTTGACTTAAATGAAGTGATGGCATTGTCTGCATGGATGACAATTAAAACTGCTGTTTTGAACTTGCC
ATTTGGTGGTGCAAAAGGTGGTATCCGTGTTGACCCACGTAAATTATCAAATCGTGAATTAGAGCGTTTAACTCGCCGTT
ATACGACTGAAATTGGTCACATTATCGGACCTCAAAAAGACATTCCTGCTCCAGACGTTGGTACAAATGCCAATATCATG
GGTTGGATGATGGATACCTACTCGACTAGCCAAGGTCACACAGTGACTGGTGTTGTAACTGGTAAACCAGTTCACTTAGG
CGGCTCTTTAGGTCGTGTGAAAGCAACTGGTCGCGGTGTATTCGTTACTGGTCGTGAAGTTGCTGCAAAAATCAACTTAC
CAATTGAAGGCGCAAAAGTTGCTGTTCAAGGTTTTGGTAATGTAGGTAGTGAAGCTGCTTTCTTATTTGTTGAATCAAAA
GCAAAAATCACTCACGTTCAAGACCACACTGGCACAATTTTCAATGCTGATGGTATTGATCTTGTTGCGCTACGTGATCA
CGTAAATGCAAACCAAGGTGTTGGTGGTTTTGCTGGTGCACAAACAATTGCAGATGAAGATTTCTGGACTGCAGAAGTAG
ACATCATTATTCCTGCTGCATTAGAAGGCCAAATTACAGTTGAGCGTGCTGAAAAACTTAAAGCTAAGTTGATTTTAGAA
GGCGCGAACGGCCCGACTTATCCTAAAGCTGAAGATGTATTGGTTGAGCGTGGCATTGTTGTAGTTCCTGACGTGGTATG
TAACGCCGGCGGTGTAACTGTAAGTTATTTCGAATGGGTTCAAGACATGGCGAGCTACTTCTGGAGCGAAGAAGAAATCA
ACGAGCGTTTAGACAAGTTGATGGTTCAAGCAATGGATGACGTTTGGAATACAGCGAACAAGAATGCTTGTACTTTACGT
ACAGCAGCTTACATCTTGGCATGTGAGCGTATCTTAAAAGCTCGTAAAGAACGCGGTATTTTCCCAGGCTAA

Upstream 100 bases:

>100_bases
TAAATAAATCAAAACATTGTTATTGCAAGGTTTTGGTTGCTGAATAATAAAGACTGCAGATTTATCACATCTGAGTCTGT
GTAGGTAGATAGGAAAAAAG

Downstream 100 bases:

>100_bases
TCTGAGGAAACTACCTTTTAGGAAAGGTGGGCCGAGCAAATATAAAGGATGTTATAGGGTTATTTGTTCGGTCCCTTTTT
CTAAATGAATTAAATTAAAG

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MSDLSYVNEHSDAWQTYLAQIDRVAPYLEHLADYIDTLKRPKRALIVDVPIVMDDGSIQHFEGYRVQHNLSRGPGKGGVR
YHPNVDLNEVMALSAWMTIKTAVLNLPFGGAKGGIRVDPRKLSNRELERLTRRYTTEIGHIIGPQKDIPAPDVGTNANIM
GWMMDTYSTSQGHTVTGVVTGKPVHLGGSLGRVKATGRGVFVTGREVAAKINLPIEGAKVAVQGFGNVGSEAAFLFVESK
AKITHVQDHTGTIFNADGIDLVALRDHVNANQGVGGFAGAQTIADEDFWTAEVDIIIPAALEGQITVERAEKLKAKLILE
GANGPTYPKAEDVLVERGIVVVPDVVCNAGGVTVSYFEWVQDMASYFWSEEEINERLDKLMVQAMDDVWNTANKNACTLR
TAAYILACERILKARKERGIFPG

Sequences:

>Translated_423_residues
MSDLSYVNEHSDAWQTYLAQIDRVAPYLEHLADYIDTLKRPKRALIVDVPIVMDDGSIQHFEGYRVQHNLSRGPGKGGVR
YHPNVDLNEVMALSAWMTIKTAVLNLPFGGAKGGIRVDPRKLSNRELERLTRRYTTEIGHIIGPQKDIPAPDVGTNANIM
GWMMDTYSTSQGHTVTGVVTGKPVHLGGSLGRVKATGRGVFVTGREVAAKINLPIEGAKVAVQGFGNVGSEAAFLFVESK
AKITHVQDHTGTIFNADGIDLVALRDHVNANQGVGGFAGAQTIADEDFWTAEVDIIIPAALEGQITVERAEKLKAKLILE
GANGPTYPKAEDVLVERGIVVVPDVVCNAGGVTVSYFEWVQDMASYFWSEEEINERLDKLMVQAMDDVWNTANKNACTLR
TAAYILACERILKARKERGIFPG
>Mature_422_residues
SDLSYVNEHSDAWQTYLAQIDRVAPYLEHLADYIDTLKRPKRALIVDVPIVMDDGSIQHFEGYRVQHNLSRGPGKGGVRY
HPNVDLNEVMALSAWMTIKTAVLNLPFGGAKGGIRVDPRKLSNRELERLTRRYTTEIGHIIGPQKDIPAPDVGTNANIMG
WMMDTYSTSQGHTVTGVVTGKPVHLGGSLGRVKATGRGVFVTGREVAAKINLPIEGAKVAVQGFGNVGSEAAFLFVESKA
KITHVQDHTGTIFNADGIDLVALRDHVNANQGVGGFAGAQTIADEDFWTAEVDIIIPAALEGQITVERAEKLKAKLILEG
ANGPTYPKAEDVLVERGIVVVPDVVCNAGGVTVSYFEWVQDMASYFWSEEEINERLDKLMVQAMDDVWNTANKNACTLRT
AAYILACERILKARKERGIFPG

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI4885281, Length=339, Percent_Identity=43.3628318584071, Blast_Score=268, Evalue=7e-72,
Organism=Homo sapiens, GI31377775, Length=342, Percent_Identity=42.9824561403509, Blast_Score=266, Evalue=4e-71,
Organism=Escherichia coli, GI1788059, Length=402, Percent_Identity=31.8407960199005, Blast_Score=179, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17544676, Length=378, Percent_Identity=37.8306878306878, Blast_Score=259, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6324951, Length=417, Percent_Identity=29.0167865707434, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6319256, Length=416, Percent_Identity=29.8076923076923, Blast_Score=149, Evalue=6e-37,
Organism=Drosophila melanogaster, GI45549226, Length=363, Percent_Identity=40.771349862259, Blast_Score=274, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24649283, Length=382, Percent_Identity=39.7905759162304, Blast_Score=272, Evalue=3e-73,
Organism=Drosophila melanogaster, GI45553475, Length=359, Percent_Identity=40.6685236768802, Blast_Score=271, Evalue=6e-73,
Organism=Drosophila melanogaster, GI24649460, Length=313, Percent_Identity=40.8945686900958, Blast_Score=232, Evalue=3e-61,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 46209; Mature: 46078

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLSYVNEHSDAWQTYLAQIDRVAPYLEHLADYIDTLKRPKRALIVDVPIVMDDGSIQH
CCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCEEE
FEGYRVQHNLSRGPGKGGVRYHPNVDLNEVMALSAWMTIKTAVLNLPFGGAKGGIRVDPR
CCCEEEEECCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCEECHH
KLSNRELERLTRRYTTEIGHIIGPQKDIPAPDVGTNANIMGWMMDTYSTSQGHTVTGVVT
HHCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEEE
GKPVHLGGSLGRVKATGRGVFVTGREVAAKINLPIEGAKVAVQGFGNVGSEAAFLFVESK
CCEEEECCCCCEEEECCCEEEEECCEEEEEECCCCCCCEEEEEECCCCCCCEEEEEEECC
AKITHVQDHTGTIFNADGIDLVALRDHVNANQGVGGFAGAQTIADEDFWTAEVDIIIPAA
CEEEEEECCCCCEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEC
LEGQITVERAEKLKAKLILEGANGPTYPKAEDVLVERGIVVVPDVVCNAGGVTVSYFEWV
CCCCEEHHHHHHCEEEEEEECCCCCCCCCHHHHHHHCCEEEECHHHCCCCCCCHHHHHHH
QDMASYFWSEEEINERLDKLMVQAMDDVWNTANKNACTLRTAAYILACERILKARKERGI
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCCC
FPG
CCC
>Mature Secondary Structure 
SDLSYVNEHSDAWQTYLAQIDRVAPYLEHLADYIDTLKRPKRALIVDVPIVMDDGSIQH
CCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCEEE
FEGYRVQHNLSRGPGKGGVRYHPNVDLNEVMALSAWMTIKTAVLNLPFGGAKGGIRVDPR
CCCEEEEECCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCEECHH
KLSNRELERLTRRYTTEIGHIIGPQKDIPAPDVGTNANIMGWMMDTYSTSQGHTVTGVVT
HHCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEEE
GKPVHLGGSLGRVKATGRGVFVTGREVAAKINLPIEGAKVAVQGFGNVGSEAAFLFVESK
CCEEEECCCCCEEEECCCEEEEECCEEEEEECCCCCCCEEEEEECCCCCCCEEEEEEECC
AKITHVQDHTGTIFNADGIDLVALRDHVNANQGVGGFAGAQTIADEDFWTAEVDIIIPAA
CEEEEEECCCCCEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEC
LEGQITVERAEKLKAKLILEGANGPTYPKAEDVLVERGIVVVPDVVCNAGGVTVSYFEWV
CCCCEEHHHHHHCEEEEEEECCCCCCCCCHHHHHHHCCEEEECHHHCCCCCCCHHHHHHH
QDMASYFWSEEEINERLDKLMVQAMDDVWNTANKNACTLRTAAYILACERILKARKERGI
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCCC
FPG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9680336; 10360571; 9135121; 9654452; 10366510 [H]