Click here to switch to the map view.

The map label for this gene is mlaE [H]

Identifier: 213158857

GI number: 213158857

Start: 3656865

End: 3657644

Strand: Reverse

Name: mlaE [H]

Synonym: AB57_3553

Alternate gene names: 213158857

Gene position: 3657644-3656865 (Counterclockwise)

Preceding gene: 213158858

Following gene: 213158856

Centisome position: 90.3

GC content: 43.08

Gene sequence:

>780_bases
ATGAATACGATTGCCTGGTTAGGTAGACTCGTTATTGAGCGGATTCGAGGCATTGGTGCTGCGGCACATATGCTCTTGCA
GATTATTTTTTCATTTCCTACCAAAGGTGGTTTTGGTCGTTTTGTTTACCAAATGCACCGCGTAGGTGTGATGTCCTTAC
TTATTATTGCTGTTTCCGGTTTGTTTATTGGCGCGGTTCTTGGCTTGCAAATGTATAGCATTTTGGTCACTTTCGGTGCT
GAGTCGATGTTGGGTACAGCGATTTCGTTAACACTACTCCGTGAGCTTGCTTCTGTGGTTGCTGCATTGCTATTTGCAGG
ACGAGCAGGTTCTGCATTAACTGCCGAAATTGGTTCAATGAAACAAAGTGAACAGCTTGCCAGTATGGAAATGATTGGTG
TTGATCCTTTAAAGCAAATCGTATCACCACGTCTTTGGGCTGGCATAGTCAGTTTGCCAATGTTAACTGTTATCTTTGCG
GCCATTGGTATTATTGGTGGCAAGATGGTCGGTGTTGACTTTTTAGGTGCTGATGAAGGCTCCTTCTGGAGTGGTATGCA
AAATACAGTGCGTTTCGGGCATGACATTTTCAATGGCACGATTATTAAAAGTATTGTTTTTGCTTTGATTTGTACATGGG
TTGCTGTTTATCAAGGTTATGCCTGTGATCCAACACCTGAAGGTATTGCAACGGCAATGACCCGGACGGTTGTGTATTCT
TCATTATGTGTTTTAGGTTTTGATTTCGTGTTGACTGCGGTCATGTTCGGAGGGATTTAA

Upstream 100 bases:

>100_bases
ATGCTTCTCCATTTGTAAAACAGTTCTTAACCGGTTCGGTAGAAGGTCCGGTTGAATATCAGTTTAGCCACCAAGCTTAT
TTAGATAACGAGGTTCGTCC

Downstream 100 bases:

>100_bases
TGAAATCACGTACTAGTGAGCTGGCCGTAGGTATCTTTGTCATTATCTTCGGTATCGCTTTATTCTTTTTAGCAATGAAA
GTGAGTGGTTTAGTGGGCAC

Product: toluene tolerance protein Ttg2B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MNTIAWLGRLVIERIRGIGAAAHMLLQIIFSFPTKGGFGRFVYQMHRVGVMSLLIIAVSGLFIGAVLGLQMYSILVTFGA
ESMLGTAISLTLLRELASVVAALLFAGRAGSALTAEIGSMKQSEQLASMEMIGVDPLKQIVSPRLWAGIVSLPMLTVIFA
AIGIIGGKMVGVDFLGADEGSFWSGMQNTVRFGHDIFNGTIIKSIVFALICTWVAVYQGYACDPTPEGIATAMTRTVVYS
SLCVLGFDFVLTAVMFGGI

Sequences:

>Translated_259_residues
MNTIAWLGRLVIERIRGIGAAAHMLLQIIFSFPTKGGFGRFVYQMHRVGVMSLLIIAVSGLFIGAVLGLQMYSILVTFGA
ESMLGTAISLTLLRELASVVAALLFAGRAGSALTAEIGSMKQSEQLASMEMIGVDPLKQIVSPRLWAGIVSLPMLTVIFA
AIGIIGGKMVGVDFLGADEGSFWSGMQNTVRFGHDIFNGTIIKSIVFALICTWVAVYQGYACDPTPEGIATAMTRTVVYS
SLCVLGFDFVLTAVMFGGI
>Mature_259_residues
MNTIAWLGRLVIERIRGIGAAAHMLLQIIFSFPTKGGFGRFVYQMHRVGVMSLLIIAVSGLFIGAVLGLQMYSILVTFGA
ESMLGTAISLTLLRELASVVAALLFAGRAGSALTAEIGSMKQSEQLASMEMIGVDPLKQIVSPRLWAGIVSLPMLTVIFA
AIGIIGGKMVGVDFLGADEGSFWSGMQNTVRFGHDIFNGTIIKSIVFALICTWVAVYQGYACDPTPEGIATAMTRTVVYS
SLCVLGFDFVLTAVMFGGI

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=259, Percent_Identity=55.984555984556, Blast_Score=276, Evalue=1e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 27558; Mature: 27558

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNTIAWLGRLVIERIRGIGAAAHMLLQIIFSFPTKGGFGRFVYQMHRVGVMSLLIIAVSG
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LFIGAVLGLQMYSILVTFGAESMLGTAISLTLLRELASVVAALLFAGRAGSALTAEIGSM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
KQSEQLASMEMIGVDPLKQIVSPRLWAGIVSLPMLTVIFAAIGIIGGKMVGVDFLGADEG
HHHHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCEEEECCCCC
SFWSGMQNTVRFGHDIFNGTIIKSIVFALICTWVAVYQGYACDPTPEGIATAMTRTVVYS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
SLCVLGFDFVLTAVMFGGI
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]