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The map label for this gene is mlaD [H]

Identifier: 213158856

GI number: 213158856

Start: 3656185

End: 3656865

Strand: Reverse

Name: mlaD [H]

Synonym: AB57_3552

Alternate gene names: 213158856

Gene position: 3656865-3656185 (Counterclockwise)

Preceding gene: 213158857

Following gene: 213158855

Centisome position: 90.28

GC content: 40.68

Gene sequence:

>681_bases
ATGAAATCACGTACTAGTGAGCTGGCCGTAGGTATCTTTGTCATTATCTTCGGTATCGCTTTATTCTTTTTAGCAATGAA
AGTGAGTGGTTTAGTGGGCACGAACTTGAGCGATGGCTACACCATGAAAGCTCAGTTTGATAACGTTAATGGTTTAAAGC
CTCGTGCTAAAGTGACGATGAGCGGTGTAACAATTGGCCGCGTTGATTCAATCACGCTTGATCCGGTTACACGTTTGGCT
ACGGTTACTTTTGATCTTGATGGAAAACTGACAAGCTTTAATGCTGAACAGTTAAAAGAAGTTCAAAAAAATGCTTTAGA
CGAATTACGTTATAGCTCTGATTATACGCAAGCGACTCCAGCGCAACAAAAAACAATGGAACAGCAACTGATCAGTAACA
TGAACTCCATCACAAGTATTGATGAAGATGCTTATATAATGGTTGCAACCAATGGTTTATTGGGTGAGAAATATTTAAAA
ATCGTACCAGGTGGTGGTCTAAACTACCTTAAACGTGGCGATACTATTTCTAATACCCAAGGAACGATGGATCTAGAAGA
TCTTATTAGTAAATTTATTACTGGTGGTGGCGCAGGTAAGGTTGCCGCTGGTTCATCCTCAGCAGAAGAAAAAGCTCCTG
CAAGTACTGACAGCAGTGCACAGCCGTCATTTGTTGAGTAA

Upstream 100 bases:

>100_bases
AAGGTATTGCAACGGCAATGACCCGGACGGTTGTGTATTCTTCATTATGTGTTTTAGGTTTTGATTTCGTGTTGACTGCG
GTCATGTTCGGAGGGATTTA

Downstream 100 bases:

>100_bases
GAGATTATTTGAAGGAGTGGTTCCAGGTGAATACGTTGTTTAAACAAACCCTTACAGCAAGTATTTTGTCTACTATGATT
GCTGGTACAGCTTTTGCTGC

Product: toluene tolerance efflux transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MKSRTSELAVGIFVIIFGIALFFLAMKVSGLVGTNLSDGYTMKAQFDNVNGLKPRAKVTMSGVTIGRVDSITLDPVTRLA
TVTFDLDGKLTSFNAEQLKEVQKNALDELRYSSDYTQATPAQQKTMEQQLISNMNSITSIDEDAYIMVATNGLLGEKYLK
IVPGGGLNYLKRGDTISNTQGTMDLEDLISKFITGGGAGKVAAGSSSAEEKAPASTDSSAQPSFVE

Sequences:

>Translated_226_residues
MKSRTSELAVGIFVIIFGIALFFLAMKVSGLVGTNLSDGYTMKAQFDNVNGLKPRAKVTMSGVTIGRVDSITLDPVTRLA
TVTFDLDGKLTSFNAEQLKEVQKNALDELRYSSDYTQATPAQQKTMEQQLISNMNSITSIDEDAYIMVATNGLLGEKYLK
IVPGGGLNYLKRGDTISNTQGTMDLEDLISKFITGGGAGKVAAGSSSAEEKAPASTDSSAQPSFVE
>Mature_226_residues
MKSRTSELAVGIFVIIFGIALFFLAMKVSGLVGTNLSDGYTMKAQFDNVNGLKPRAKVTMSGVTIGRVDSITLDPVTRLA
TVTFDLDGKLTSFNAEQLKEVQKNALDELRYSSDYTQATPAQQKTMEQQLISNMNSITSIDEDAYIMVATNGLLGEKYLK
IVPGGGLNYLKRGDTISNTQGTMDLEDLISKFITGGGAGKVAAGSSSAEEKAPASTDSSAQPSFVE

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG1463

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, periplasmic component

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein; Periplasmic side (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaD family [H]

Homologues:

Organism=Escherichia coli, GI1789584, Length=224, Percent_Identity=30.3571428571429, Blast_Score=90, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003399 [H]

Pfam domain/function: PF02470 MCE [H]

EC number: NA

Molecular weight: Translated: 24115; Mature: 24115

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSRTSELAVGIFVIIFGIALFFLAMKVSGLVGTNLSDGYTMKAQFDNVNGLKPRAKVTM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCEEEE
SGVTIGRVDSITLDPVTRLATVTFDLDGKLTSFNAEQLKEVQKNALDELRYSSDYTQATP
CCEEECCCCCEEECCHHEEEEEEEECCCCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCC
AQQKTMEQQLISNMNSITSIDEDAYIMVATNGLLGEKYLKIVPGGGLNYLKRGDTISNTQ
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEECCCCCHHHHCCCCCCCCC
GTMDLEDLISKFITGGGAGKVAAGSSSAEEKAPASTDSSAQPSFVE
CCCCHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKSRTSELAVGIFVIIFGIALFFLAMKVSGLVGTNLSDGYTMKAQFDNVNGLKPRAKVTM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCEEEE
SGVTIGRVDSITLDPVTRLATVTFDLDGKLTSFNAEQLKEVQKNALDELRYSSDYTQATP
CCEEECCCCCEEECCHHEEEEEEEECCCCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCC
AQQKTMEQQLISNMNSITSIDEDAYIMVATNGLLGEKYLKIVPGGGLNYLKRGDTISNTQ
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEECCCCCHHHHCCCCCCCCC
GTMDLEDLISKFITGGGAGKVAAGSSSAEEKAPASTDSSAQPSFVE
CCCCHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]