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The map label for this gene is fmt [H]

Identifier: 213158778

GI number: 213158778

Start: 4038435

End: 4039397

Strand: Direct

Name: fmt [H]

Synonym: AB57_3912

Alternate gene names: 213158778

Gene position: 4038435-4039397 (Clockwise)

Preceding gene: 213158774

Following gene: 213158779

Centisome position: 99.7

GC content: 42.89

Gene sequence:

>963_bases
GTGAAAATTATTTTTGCTGGCACCCCCGAATTTGCTGCAACAGCATTGGCGGCTCTACTAAAAACTTCCCATGAAATTAT
CGCGGTTTATACTCAACCAGACCGTAAAGCAGGTCGTGGACAAAAACTAACACCGTCTCCTGTAAAACAGCTTGCGCTGG
AACATAATATTCCTGTTTATCAACCACTTCACTTCAAAGCTTCAACTGAAGAAGGCTTGGCAGCTCAACAAGAACTTGCT
GCTTTAGGCGCTGATGTCATGGTAGTTGCAGCATATGGTTTAATTCTTCCACAAGCTGTGCTCGATACACCTAAATATGG
CTGTTTAAATATTCACGGCTCATTATTACCACGCTGGCGCGGTGCAGCACCGATTCAGCGTGCAATTGCAACAGGTGACG
ACGAAACTGGTATCACCATCATGCAAATGGCGGCGGGTTTAGATACAGGCGATATGATGTATAAAACCTATTGTCCAATC
GCTTCCGAAGATACATCAGCGACGTTGCATGACAAACTTGCAGCGCAAGGAGCAACAGCAATTTGTGCGGTACTTGAGTC
AGAAGAAACTCTACAAAAATATTTGGCAGAACGCGAAGTTCAAGACGAAAGCTTAACAGTCTATGCGCATAAACTCGTGA
AATCAGAAGCTCGTATTGATTGGTCTATGAATGCAGTTCAAGTTGACCGCAATATCCGTGCATTCAACCCATGGCCTGTT
GCCTTTATTCAACTTGATGAAAATAATGCGCTGCGTGTTTGGAATTCAATAATTTCTAGTCAAAGCAAAGTAAATGCTCA
AGCTGGTGAAATTATTGCAATCGATAAACAGGGCGTACATGTTGCTTGCGGTGAAAATACATTTATTTGTTTAACGAGTG
TGCAGTGGCCGGGTGGTAAAGCATTAAATGCTCAACAAATTGCACAAACTCAAAAACTTCACGTAGGACAGATTCTGCCA
TGA

Upstream 100 bases:

>100_bases
TTGTTCACTATTTATGGCTTTAGCTCTCCTTTGTACCTCGATTTAGGGCTTCAATCGTTTTTCACGTATACTAGCGTTAA
ACTTTTTTTGGATTGCATTT

Downstream 100 bases:

>100_bases
GTCAGGTTACTTCAAATTCATCACGTTTTAACCTGCGCGCTCAAGTCGTCCAGACCTTGCTAAAAGTTCAGCAAGGTCAA
TCGTTGGCTAGTATTTTAAA

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MKIIFAGTPEFAATALAALLKTSHEIIAVYTQPDRKAGRGQKLTPSPVKQLALEHNIPVYQPLHFKASTEEGLAAQQELA
ALGADVMVVAAYGLILPQAVLDTPKYGCLNIHGSLLPRWRGAAPIQRAIATGDDETGITIMQMAAGLDTGDMMYKTYCPI
ASEDTSATLHDKLAAQGATAICAVLESEETLQKYLAEREVQDESLTVYAHKLVKSEARIDWSMNAVQVDRNIRAFNPWPV
AFIQLDENNALRVWNSIISSQSKVNAQAGEIIAIDKQGVHVACGENTFICLTSVQWPGGKALNAQQIAQTQKLHVGQILP

Sequences:

>Translated_320_residues
MKIIFAGTPEFAATALAALLKTSHEIIAVYTQPDRKAGRGQKLTPSPVKQLALEHNIPVYQPLHFKASTEEGLAAQQELA
ALGADVMVVAAYGLILPQAVLDTPKYGCLNIHGSLLPRWRGAAPIQRAIATGDDETGITIMQMAAGLDTGDMMYKTYCPI
ASEDTSATLHDKLAAQGATAICAVLESEETLQKYLAEREVQDESLTVYAHKLVKSEARIDWSMNAVQVDRNIRAFNPWPV
AFIQLDENNALRVWNSIISSQSKVNAQAGEIIAIDKQGVHVACGENTFICLTSVQWPGGKALNAQQIAQTQKLHVGQILP
>Mature_320_residues
MKIIFAGTPEFAATALAALLKTSHEIIAVYTQPDRKAGRGQKLTPSPVKQLALEHNIPVYQPLHFKASTEEGLAAQQELA
ALGADVMVVAAYGLILPQAVLDTPKYGCLNIHGSLLPRWRGAAPIQRAIATGDDETGITIMQMAAGLDTGDMMYKTYCPI
ASEDTSATLHDKLAAQGATAICAVLESEETLQKYLAEREVQDESLTVYAHKLVKSEARIDWSMNAVQVDRNIRAFNPWPV
AFIQLDENNALRVWNSIISSQSKVNAQAGEIIAIDKQGVHVACGENTFICLTSVQWPGGKALNAQQIAQTQKLHVGQILP

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family [H]

Homologues:

Organism=Homo sapiens, GI21614513, Length=264, Percent_Identity=26.8939393939394, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI238814322, Length=321, Percent_Identity=23.3644859813084, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI164663775, Length=247, Percent_Identity=30.7692307692308, Blast_Score=95, Evalue=9e-20,
Organism=Escherichia coli, GI1789683, Length=313, Percent_Identity=53.3546325878594, Blast_Score=332, Evalue=2e-92,
Organism=Escherichia coli, GI1788589, Length=323, Percent_Identity=28.7925696594427, Blast_Score=112, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI133930964, Length=254, Percent_Identity=31.1023622047244, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI45550868, Length=236, Percent_Identity=30.0847457627119, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI28571984, Length=153, Percent_Identity=34.640522875817, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24585660, Length=224, Percent_Identity=29.0178571428571, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR015518 [H]

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N [H]

EC number: =2.1.2.9 [H]

Molecular weight: Translated: 34637; Mature: 34637

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIFAGTPEFAATALAALLKTSHEIIAVYTQPDRKAGRGQKLTPSPVKQLALEHNIPVY
CEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCC
QPLHFKASTEEGLAAQQELAALGADVMVVAAYGLILPQAVLDTPKYGCLNIHGSLLPRWR
CCEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCC
GAAPIQRAIATGDDETGITIMQMAAGLDTGDMMYKTYCPIASEDTSATLHDKLAAQGATA
CCCHHHHHHHCCCCCCCEEEEHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCHHH
ICAVLESEETLQKYLAEREVQDESLTVYAHKLVKSEARIDWSMNAVQVDRNIRAFNPWPV
HHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEECCCCE
AFIQLDENNALRVWNSIISSQSKVNAQAGEIIAIDKQGVHVACGENTFICLTSVQWPGGK
EEEEECCCCHHHHHHHHHCCHHHCCCCCCCEEEEECCCEEEEECCCEEEEEEEEECCCCC
ALNAQQIAQTQKLHVGQILP
CCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKIIFAGTPEFAATALAALLKTSHEIIAVYTQPDRKAGRGQKLTPSPVKQLALEHNIPVY
CEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCC
QPLHFKASTEEGLAAQQELAALGADVMVVAAYGLILPQAVLDTPKYGCLNIHGSLLPRWR
CCEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCC
GAAPIQRAIATGDDETGITIMQMAAGLDTGDMMYKTYCPIASEDTSATLHDKLAAQGATA
CCCHHHHHHHCCCCCCCEEEEHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCHHH
ICAVLESEETLQKYLAEREVQDESLTVYAHKLVKSEARIDWSMNAVQVDRNIRAFNPWPV
HHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEECCCCE
AFIQLDENNALRVWNSIISSQSKVNAQAGEIIAIDKQGVHVACGENTFICLTSVQWPGGK
EEEEECCCCHHHHHHHHHCCHHHCCCCCCCEEEEECCCEEEEECCCEEEEEEEEECCCCC
ALNAQQIAQTQKLHVGQILP
CCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA