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The map label for this gene is argT [H]

Identifier: 213158701

GI number: 213158701

Start: 2746669

End: 2747454

Strand: Reverse

Name: argT [H]

Synonym: AB57_2659

Alternate gene names: 213158701

Gene position: 2747454-2746669 (Counterclockwise)

Preceding gene: 213158702

Following gene: 213158700

Centisome position: 67.83

GC content: 39.82

Gene sequence:

>786_bases
ATGGCAAGCTTTATGAAAAAAATATTCGTTTCAACAACCTTAGTTCTGGCTGCTGTTGCGACGAATGTACAAGCAAAGGA
TTGGAAGGTGATTCGTTTTGGTACTGAGTCGTCTTATGCGCCGTTTGAATATAAAACACCGGATGGCAAGTTAACTGGTT
TTGATGTTGATTTGGGTAATGCTATTTGTGCAAAACTCAAAGCTAAATGTGTTTGGGTTGAAAACTCTTTTGACGGGATG
ATTCCCGCGCTTAAAGCGAAAAAATTTGATGGCATTCTTTCATCAATGACTGTTACTGATGAACGTGCAAAACAAATTTT
ATTTAGTAGCAAAATCTACAACACACCAACGCGTATGGTTGCAAAAAAAGGTTCACCGTTATTACCAACACCTACTTCAT
TAAAAGGTAAGCGTGTCGGTGTACAGCAGGGCACAATTCAAGAAACTTACGCTAAAACCTATTGGGCTCCAAAAGGTGTA
AGTGTTGTCCCTTATCCAACTCAAGACTTGATTTACCAAGACATGATGTCTGGTCGTTTGGATGCAACTTTACAAGATGC
AATCATGGTTGATGGCGCTTTCTTAAAACAACCGAAGGGTAAAAACTTCTCTTTTGCCGGCGGCAATGTGGTTGATGTCA
AAACATTGGGTGTAGGTGCGGCTATCGGTTTACGTAAAGAAGATGCAGACTTGAAAGCGAATATTGACAAGGCACTCGCT
GCAATCATTGCGGATGGTACATACAAGAAACTCGAGAAAAAATACTTCTCTTTTAGCATTTACTAA

Upstream 100 bases:

>100_bases
TAACTTATGGTATAGAAATTGCTGTTATTTCAGCAGATGAGTAAGTTCGAAATACTCAAGTTTTTATCACCTTATTTGTG
TTTTAAGGAGAAGCTTCAAG

Downstream 100 bases:

>100_bases
TGATGTGAAAAATGTCGGCTGAGTGCCGACATTTTTAAATCTATTCTCATCTCATCAATATTAGATATTGTTTTTAAGGA
GAAGATGAGTCATGTCTTTT

Product: histidine-binding periplasmic protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MASFMKKIFVSTTLVLAAVATNVQAKDWKVIRFGTESSYAPFEYKTPDGKLTGFDVDLGNAICAKLKAKCVWVENSFDGM
IPALKAKKFDGILSSMTVTDERAKQILFSSKIYNTPTRMVAKKGSPLLPTPTSLKGKRVGVQQGTIQETYAKTYWAPKGV
SVVPYPTQDLIYQDMMSGRLDATLQDAIMVDGAFLKQPKGKNFSFAGGNVVDVKTLGVGAAIGLRKEDADLKANIDKALA
AIIADGTYKKLEKKYFSFSIY

Sequences:

>Translated_261_residues
MASFMKKIFVSTTLVLAAVATNVQAKDWKVIRFGTESSYAPFEYKTPDGKLTGFDVDLGNAICAKLKAKCVWVENSFDGM
IPALKAKKFDGILSSMTVTDERAKQILFSSKIYNTPTRMVAKKGSPLLPTPTSLKGKRVGVQQGTIQETYAKTYWAPKGV
SVVPYPTQDLIYQDMMSGRLDATLQDAIMVDGAFLKQPKGKNFSFAGGNVVDVKTLGVGAAIGLRKEDADLKANIDKALA
AIIADGTYKKLEKKYFSFSIY
>Mature_260_residues
ASFMKKIFVSTTLVLAAVATNVQAKDWKVIRFGTESSYAPFEYKTPDGKLTGFDVDLGNAICAKLKAKCVWVENSFDGMI
PALKAKKFDGILSSMTVTDERAKQILFSSKIYNTPTRMVAKKGSPLLPTPTSLKGKRVGVQQGTIQETYAKTYWAPKGVS
VVPYPTQDLIYQDMMSGRLDATLQDAIMVDGAFLKQPKGKNFSFAGGNVVDVKTLGVGAAIGLRKEDADLKANIDKALAA
IIADGTYKKLEKKYFSFSIY

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=256, Percent_Identity=50, Blast_Score=273, Evalue=9e-75,
Organism=Escherichia coli, GI1788648, Length=231, Percent_Identity=50.6493506493506, Blast_Score=260, Evalue=7e-71,
Organism=Escherichia coli, GI1787085, Length=245, Percent_Identity=33.8775510204082, Blast_Score=148, Evalue=3e-37,
Organism=Escherichia coli, GI1787088, Length=253, Percent_Identity=31.2252964426877, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1788228, Length=239, Percent_Identity=31.7991631799163, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1787031, Length=256, Percent_Identity=32.421875, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1786876, Length=241, Percent_Identity=27.8008298755187, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28486; Mature: 28355

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASFMKKIFVSTTLVLAAVATNVQAKDWKVIRFGTESSYAPFEYKTPDGKLTGFDVDLGN
CHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEECCCCCEEEEEEECCH
AICAKLKAKCVWVENSFDGMIPALKAKKFDGILSSMTVTDERAKQILFSSKIYNTPTRMV
HHHHHHEEEEEEEECCCCCCCHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHCCCHHHHH
AKKGSPLLPTPTSLKGKRVGVQQGTIQETYAKTYWAPKGVSVVPYPTQDLIYQDMMSGRL
HCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHCCCC
DATLQDAIMVDGAFLKQPKGKNFSFAGGNVVDVKTLGVGAAIGLRKEDADLKANIDKALA
CCHHHHHHEECCHHHCCCCCCCEEECCCCEEEEEECCCCEEECCCCCCCCHHHHHHHHHH
AIIADGTYKKLEKKYFSFSIY
HHHCCCHHHHHHHHHEEEECC
>Mature Secondary Structure 
ASFMKKIFVSTTLVLAAVATNVQAKDWKVIRFGTESSYAPFEYKTPDGKLTGFDVDLGN
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEECCCCCEEEEEEECCH
AICAKLKAKCVWVENSFDGMIPALKAKKFDGILSSMTVTDERAKQILFSSKIYNTPTRMV
HHHHHHEEEEEEEECCCCCCCHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHCCCHHHHH
AKKGSPLLPTPTSLKGKRVGVQQGTIQETYAKTYWAPKGVSVVPYPTQDLIYQDMMSGRL
HCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHCCCC
DATLQDAIMVDGAFLKQPKGKNFSFAGGNVVDVKTLGVGAAIGLRKEDADLKANIDKALA
CCHHHHHHEECCHHHCCCCCCCEEECCCCEEEEEECCCCEEECCCCCCCCHHHHHHHHHH
AIIADGTYKKLEKKYFSFSIY
HHHCCCHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]