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The map label for this gene is ghrB [H]

Identifier: 213158650

GI number: 213158650

Start: 2696196

End: 2697161

Strand: Reverse

Name: ghrB [H]

Synonym: AB57_2604

Alternate gene names: 213158650

Gene position: 2697161-2696196 (Counterclockwise)

Preceding gene: 213158651

Following gene: 213158649

Centisome position: 66.59

GC content: 38.3

Gene sequence:

>966_bases
ATGAAAAAGGTTGTGGTTTTTAGCCAAATTGATGAGGAAATTTTGTCACGGTTACAGCAGGATTACCATGTTGTCGTATT
GAACCCTAAACTGGGTGATATTAATGAACAGATACGACAAGAAGTAGTAGATGCTGATGGCATGATTGGGGCGGGTCGTT
TGCTCAATGAAAGTAATCTTTCTCCTGCCCAAAAATTAAAAATTATTTCTTCTGTGTCTGTAGGTTATGACAATTATGAT
GTTGGTTATTTAAATCAGAAAAAAATCTGGTTAGCAAACACACCTCATGTTTTAACTGAAACGACCGCAGATCTTGCTTT
TACTTTGTTATTAAGTGCAGCCAGAAAAGTTCCTTTTCTAGATCACTGGACTAAACAAGGTGAGTGGAAAAGAACAGTTG
GGCCACAGCAGTTTGGTCTCGATGTTTTTGGAAAAACATTAGGTATTATTGGACTTGGTAATATTGGTGCTGCAATTGCA
CGTCGTGGCTTTTATGGTTTTAACATGAATATTGTTTACCATAATCGTCGCGAGAAACCTGAGTTGGCGGAACCTTTAAA
AGCTCAATATCTGGGGTTGGAAGAATTATTACAACAATCTGATTTTGTGGTGACGGCTGTTGATTTAAATGCGGAATCTA
AAGCTTTAATGGGCAAGGCTCAATTTGAGCTTATGCAAAAGCATGCCATATTTATTAATATTGCGCGTGGCTCAGTTGTC
GATGAGCAAGCCCTAATTGAAGCATTGCAAAATGAAGTTATTTTTGCTGCTGGTTTAGACGTCTATGAGAAAGAACCTCT
GCAAGAATCTGCACTTTTCACTTTGCCGAACGTTGTCACTTTGCCTCACGTAGGTTCTGCTACTGCTGAAACACGTAAGA
AAATGGCAAATTTAGCTTATAAAAACTTGGTGGAAGCACTAGAGGACAAGACGCCTAGATATTTAGTGAATCCTAATTTT
GTGTAA

Upstream 100 bases:

>100_bases
TTATTCATTTAGCTAGTTTAAAAGAAATGTTAGATAAGCCTCTTCTTAAGAAAAGGTTGTGGCAACTTATGCAAGATAAC
AATGAGTAGTAGGGATTTAC

Downstream 100 bases:

>100_bases
AGGATGTATAACACTTCATTGCAAATATAGTCTATTTTCAATGTTATAGTGAAAGCCTGTTGATATGATGCAAAATAACT
GAAGCATGATATTGATGGGT

Product: 2-ketogluconate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MKKVVVFSQIDEEILSRLQQDYHVVVLNPKLGDINEQIRQEVVDADGMIGAGRLLNESNLSPAQKLKIISSVSVGYDNYD
VGYLNQKKIWLANTPHVLTETTADLAFTLLLSAARKVPFLDHWTKQGEWKRTVGPQQFGLDVFGKTLGIIGLGNIGAAIA
RRGFYGFNMNIVYHNRREKPELAEPLKAQYLGLEELLQQSDFVVTAVDLNAESKALMGKAQFELMQKHAIFINIARGSVV
DEQALIEALQNEVIFAAGLDVYEKEPLQESALFTLPNVVTLPHVGSATAETRKKMANLAYKNLVEALEDKTPRYLVNPNF
V

Sequences:

>Translated_321_residues
MKKVVVFSQIDEEILSRLQQDYHVVVLNPKLGDINEQIRQEVVDADGMIGAGRLLNESNLSPAQKLKIISSVSVGYDNYD
VGYLNQKKIWLANTPHVLTETTADLAFTLLLSAARKVPFLDHWTKQGEWKRTVGPQQFGLDVFGKTLGIIGLGNIGAAIA
RRGFYGFNMNIVYHNRREKPELAEPLKAQYLGLEELLQQSDFVVTAVDLNAESKALMGKAQFELMQKHAIFINIARGSVV
DEQALIEALQNEVIFAAGLDVYEKEPLQESALFTLPNVVTLPHVGSATAETRKKMANLAYKNLVEALEDKTPRYLVNPNF
V
>Mature_321_residues
MKKVVVFSQIDEEILSRLQQDYHVVVLNPKLGDINEQIRQEVVDADGMIGAGRLLNESNLSPAQKLKIISSVSVGYDNYD
VGYLNQKKIWLANTPHVLTETTADLAFTLLLSAARKVPFLDHWTKQGEWKRTVGPQQFGLDVFGKTLGIIGLGNIGAAIA
RRGFYGFNMNIVYHNRREKPELAEPLKAQYLGLEELLQQSDFVVTAVDLNAESKALMGKAQFELMQKHAIFINIARGSVV
DEQALIEALQNEVIFAAGLDVYEKEPLQESALFTLPNVVTLPHVGSATAETRKKMANLAYKNLVEALEDKTPRYLVNPNF
V

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrB subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=245, Percent_Identity=42.4489795918367, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI23308577, Length=318, Percent_Identity=29.559748427673, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI61743967, Length=294, Percent_Identity=28.9115646258503, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI145580575, Length=294, Percent_Identity=28.5714285714286, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI145580578, Length=294, Percent_Identity=28.5714285714286, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI4557499, Length=294, Percent_Identity=28.5714285714286, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI4557497, Length=294, Percent_Identity=28.9115646258503, Blast_Score=115, Evalue=5e-26,
Organism=Escherichia coli, GI87082289, Length=321, Percent_Identity=45.7943925233645, Blast_Score=273, Evalue=7e-75,
Organism=Escherichia coli, GI1789279, Length=269, Percent_Identity=30.1115241635688, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787645, Length=200, Percent_Identity=29, Blast_Score=89, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17532191, Length=311, Percent_Identity=30.2250803858521, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI25147481, Length=296, Percent_Identity=23.9864864864865, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324055, Length=256, Percent_Identity=37.890625, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6322116, Length=255, Percent_Identity=30.1960784313725, Blast_Score=110, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6320925, Length=292, Percent_Identity=28.0821917808219, Blast_Score=110, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6321253, Length=292, Percent_Identity=26.027397260274, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6325144, Length=195, Percent_Identity=26.6666666666667, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324964, Length=288, Percent_Identity=23.9583333333333, Blast_Score=66, Evalue=6e-12,
Organism=Drosophila melanogaster, GI45551003, Length=313, Percent_Identity=35.1437699680511, Blast_Score=164, Evalue=5e-41,
Organism=Drosophila melanogaster, GI45552429, Length=315, Percent_Identity=35.2380952380952, Blast_Score=164, Evalue=5e-41,
Organism=Drosophila melanogaster, GI28574284, Length=313, Percent_Identity=35.1437699680511, Blast_Score=164, Evalue=6e-41,
Organism=Drosophila melanogaster, GI24585514, Length=313, Percent_Identity=35.1437699680511, Blast_Score=164, Evalue=6e-41,
Organism=Drosophila melanogaster, GI28574282, Length=313, Percent_Identity=35.1437699680511, Blast_Score=164, Evalue=6e-41,
Organism=Drosophila melanogaster, GI28574286, Length=276, Percent_Identity=37.3188405797101, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI28571528, Length=275, Percent_Identity=36, Blast_Score=158, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24585516, Length=278, Percent_Identity=34.8920863309352, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI19921140, Length=319, Percent_Identity=27.2727272727273, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24646446, Length=299, Percent_Identity=29.7658862876254, Blast_Score=118, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24646448, Length=299, Percent_Identity=29.7658862876254, Blast_Score=118, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24646452, Length=299, Percent_Identity=29.7658862876254, Blast_Score=118, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24646450, Length=299, Percent_Identity=29.7658862876254, Blast_Score=118, Evalue=4e-27,
Organism=Drosophila melanogaster, GI62472511, Length=299, Percent_Identity=29.7658862876254, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 35766; Mature: 35766

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVVVFSQIDEEILSRLQQDYHVVVLNPKLGDINEQIRQEVVDADGMIGAGRLLNESNL
CCEEEEHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHCCCCCC
SPAQKLKIISSVSVGYDNYDVGYLNQKKIWLANTPHVLTETTADLAFTLLLSAARKVPFL
CHHHHHHHHHHHCCCCCCCCCCEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCH
DHWTKQGEWKRTVGPQQFGLDVFGKTLGIIGLGNIGAAIARRGFYGFNMNIVYHNRREKP
HHHCCCCCCCCCCCCHHHCHHHHHHHHCEEEECHHHHHHHHCCCCEEEEEEEECCCCCCC
ELAEPLKAQYLGLEELLQQSDFVVTAVDLNAESKALMGKAQFELMQKHAIFINIARGSVV
HHHHHHHHHHCCHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCEEEEEECCCCCC
DEQALIEALQNEVIFAAGLDVYEKEPLQESALFTLPNVVTLPHVGSATAETRKKMANLAY
CHHHHHHHHHHCEEEEECCCHHHCCCCHHHHHEECCCEEECCCCCCHHHHHHHHHHHHHH
KNLVEALEDKTPRYLVNPNFV
HHHHHHHHCCCCEEEECCCCC
>Mature Secondary Structure
MKKVVVFSQIDEEILSRLQQDYHVVVLNPKLGDINEQIRQEVVDADGMIGAGRLLNESNL
CCEEEEHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHCCCCCC
SPAQKLKIISSVSVGYDNYDVGYLNQKKIWLANTPHVLTETTADLAFTLLLSAARKVPFL
CHHHHHHHHHHHCCCCCCCCCCEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCH
DHWTKQGEWKRTVGPQQFGLDVFGKTLGIIGLGNIGAAIARRGFYGFNMNIVYHNRREKP
HHHCCCCCCCCCCCCHHHCHHHHHHHHCEEEECHHHHHHHHCCCCEEEEEEEECCCCCCC
ELAEPLKAQYLGLEELLQQSDFVVTAVDLNAESKALMGKAQFELMQKHAIFINIARGSVV
HHHHHHHHHHCCHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCEEEEEECCCCCC
DEQALIEALQNEVIFAAGLDVYEKEPLQESALFTLPNVVTLPHVGSATAETRKKMANLAY
CHHHHHHHHHHCEEEEECCCHHHCCCCHHHHHEECCCEEECCCCCCHHHHHHHHHHHHHH
KNLVEALEDKTPRYLVNPNFV
HHHHHHHHCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA