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The map label for this gene is puuA [H]

Identifier: 213158518

GI number: 213158518

Start: 2560868

End: 2562292

Strand: Direct

Name: puuA [H]

Synonym: AB57_2469

Alternate gene names: 213158518

Gene position: 2560868-2562292 (Clockwise)

Preceding gene: 213158516

Following gene: 213158528

Centisome position: 63.22

GC content: 36.28

Gene sequence:

>1425_bases
ATGAGTACGCTTATTTACCCAAATTTAAACTTAAAAGATCCTCACTTTAAGGCATTTAAAATATCAGATGATTTTATTCC
ACAGACACAAAGTCATACGTTCAACTCAGAGCTTTTTTTAAAAGAAGTTGATGAATATTTAAGTTTATATCCTCACACTC
AGCATATTGATATTTGTCTACATGACTTAAACGGGCATATTCGTGGTAAACGAATTGATGTTAAAAGTCTAAAAAATCTA
TCGAATGGATGCTATTTTCCTCTTTCTGTTTATGCCATGAGCCTTGATGGGAAAGTGATTGAAGAAACAGGTCTGGGAAA
ATATATTGGTGAACCAGATAGACTTTGCCTGCCCATTTTAGGCAGCTTGCAACCCAGTGCCTTATCCCCAGAATTAAATG
CTCAGCTTTACTTATCCATGCAAGAAGAAGATGGTTCTGGCTGTCGCTATGAACCACGTAATATATTAAAAAAACTATTA
AATCAGTTACATGCTAATAATTATTTCCCTGTCATGGCTGCCGAGCTAGAGTTTTATCTTTTCTCACCTCAGCATCAATC
CGAGATATGTTCAGAAAACCAATGTTTTGATATAGATGCGCCAAATAATTATCAGCAAGTTCTGGATGAAGTTGAAAAAG
CAGCCCTACTACAATCAATTGAAATTACCGCTATTGTTGCAGAGTCATCCCCTGGACAATATGAATTAAACCTTCAACAC
AACCATGACATTTTAAAACTCTGCGATCAAATTAATGCCTTAAAAAGAATTGTTAAACAAGTTGCAAGAAAGCATGACTT
AACCGCTTGTTTCATGGCGAAACCAAACTTGGCAAAGGCTGGTAGTGGTATGCATTTTCATATGAGTCTGCTTAATCAAT
ACCATCAAAATATATTTAGCTCAGAAGAAGAAAAAGATGAGCTTGCAACCAAATTATTGAGCGCAATTAGTGGACTCATT
GAGTTAATGCCTGCATCTATGGCGATTTTAGCGCCTAATATTAATTCTTATAGACGTTTTAAAATTGGTCATCACGTGCC
ATTAGAAGCCAATTGGGATACTAATAACCGAAATGTTGCAATCCGAATTCCATGCTCAGACGCACAAAATCAACGCTTAG
AATATCGTGTTGCTGGAGCGGACTGTAATCCCTATTTGGTAACAGCGACTATTTTAGCGGGCGCGTCTTATGGCTTATCT
CATAAGTTACCGCTACCAAAACCTGCACATCTTTTAAAATTTCCAGACGAGCACATTTTATTAGCTAACAACCAACCAGA
GGCCTTAAAAATATTTAAAGGAAGTCTTATTTTAAAAGGATATTTAGGCGCTGATTTTGTCGAACATTGGTACACAGTTA
AGCAAGCTGAATATCAAAGTATTTACAGCCAAATGACTGAAGCAGAACAACATTGGAATATTTGA

Upstream 100 bases:

>100_bases
ACTTATAAATAAACTATTTATTATTTTACTGTTTTTTTACGGGATTAACGTCAAAGGCTACGGTAGGATTTGAGCTAAAT
AACCACCAAGGTTTTTTATA

Downstream 100 bases:

>100_bases
ATAAAAAAAGCAAAAAACTATAAACCACTTTGCAAACCCTTAGGGAGAAATACCCTAAGGTTTTTTTACATCTAATTAAT
GATTTTTCTGCTTAGCTAAA

Product: glutamine synthetase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSTLIYPNLNLKDPHFKAFKISDDFIPQTQSHTFNSELFLKEVDEYLSLYPHTQHIDICLHDLNGHIRGKRIDVKSLKNL
SNGCYFPLSVYAMSLDGKVIEETGLGKYIGEPDRLCLPILGSLQPSALSPELNAQLYLSMQEEDGSGCRYEPRNILKKLL
NQLHANNYFPVMAAELEFYLFSPQHQSEICSENQCFDIDAPNNYQQVLDEVEKAALLQSIEITAIVAESSPGQYELNLQH
NHDILKLCDQINALKRIVKQVARKHDLTACFMAKPNLAKAGSGMHFHMSLLNQYHQNIFSSEEEKDELATKLLSAISGLI
ELMPASMAILAPNINSYRRFKIGHHVPLEANWDTNNRNVAIRIPCSDAQNQRLEYRVAGADCNPYLVTATILAGASYGLS
HKLPLPKPAHLLKFPDEHILLANNQPEALKIFKGSLILKGYLGADFVEHWYTVKQAEYQSIYSQMTEAEQHWNI

Sequences:

>Translated_474_residues
MSTLIYPNLNLKDPHFKAFKISDDFIPQTQSHTFNSELFLKEVDEYLSLYPHTQHIDICLHDLNGHIRGKRIDVKSLKNL
SNGCYFPLSVYAMSLDGKVIEETGLGKYIGEPDRLCLPILGSLQPSALSPELNAQLYLSMQEEDGSGCRYEPRNILKKLL
NQLHANNYFPVMAAELEFYLFSPQHQSEICSENQCFDIDAPNNYQQVLDEVEKAALLQSIEITAIVAESSPGQYELNLQH
NHDILKLCDQINALKRIVKQVARKHDLTACFMAKPNLAKAGSGMHFHMSLLNQYHQNIFSSEEEKDELATKLLSAISGLI
ELMPASMAILAPNINSYRRFKIGHHVPLEANWDTNNRNVAIRIPCSDAQNQRLEYRVAGADCNPYLVTATILAGASYGLS
HKLPLPKPAHLLKFPDEHILLANNQPEALKIFKGSLILKGYLGADFVEHWYTVKQAEYQSIYSQMTEAEQHWNI
>Mature_473_residues
STLIYPNLNLKDPHFKAFKISDDFIPQTQSHTFNSELFLKEVDEYLSLYPHTQHIDICLHDLNGHIRGKRIDVKSLKNLS
NGCYFPLSVYAMSLDGKVIEETGLGKYIGEPDRLCLPILGSLQPSALSPELNAQLYLSMQEEDGSGCRYEPRNILKKLLN
QLHANNYFPVMAAELEFYLFSPQHQSEICSENQCFDIDAPNNYQQVLDEVEKAALLQSIEITAIVAESSPGQYELNLQHN
HDILKLCDQINALKRIVKQVARKHDLTACFMAKPNLAKAGSGMHFHMSLLNQYHQNIFSSEEEKDELATKLLSAISGLIE
LMPASMAILAPNINSYRRFKIGHHVPLEANWDTNNRNVAIRIPCSDAQNQRLEYRVAGADCNPYLVTATILAGASYGLSH
KLPLPKPAHLLKFPDEHILLANNQPEALKIFKGSLILKGYLGADFVEHWYTVKQAEYQSIYSQMTEAEQHWNI

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=324, Percent_Identity=23.4567901234568, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI87081870, Length=463, Percent_Identity=45.3563714902808, Blast_Score=406, Evalue=1e-114,
Organism=Escherichia coli, GI1790301, Length=176, Percent_Identity=34.0909090909091, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 53635; Mature: 53504

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLIYPNLNLKDPHFKAFKISDDFIPQTQSHTFNSELFLKEVDEYLSLYPHTQHIDICL
CCEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEE
HDLNGHIRGKRIDVKSLKNLSNGCYFPLSVYAMSLDGKVIEETGLGKYIGEPDRLCLPIL
EECCCCCCCCEECHHHHHHCCCCCEEEHEEEEEECCCEEEECCCCCCCCCCCCCCHHHHH
GSLQPSALSPELNAQLYLSMQEEDGSGCRYEPRNILKKLLNQLHANNYFPVMAAELEFYL
CCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEE
FSPQHQSEICSENQCFDIDAPNNYQQVLDEVEKAALLQSIEITAIVAESSPGQYELNLQH
ECCCHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHEEEEEEEEECCCCEEEEEECC
NHDILKLCDQINALKRIVKQVARKHDLTACFMAKPNLAKAGSGMHFHMSLLNQYHQNIFS
CCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHCCCCCEEHHHHHHHHHHHHHC
SEEEKDELATKLLSAISGLIELMPASMAILAPNINSYRRFKIGHHVPLEANWDTNNRNVA
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEECCCCCCEECCCCCCCCEEE
IRIPCSDAQNQRLEYRVAGADCNPYLVTATILAGASYGLSHKLPLPKPAHLLKFPDEHIL
EEEECCCCCCCCEEEEEECCCCCCEEEEEHHHHCCCCCCCCCCCCCCCHHHHCCCCCEEE
LANNQPEALKIFKGSLILKGYLGADFVEHWYTVKQAEYQSIYSQMTEAEQHWNI
EECCCCCEEEEEECCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STLIYPNLNLKDPHFKAFKISDDFIPQTQSHTFNSELFLKEVDEYLSLYPHTQHIDICL
CEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEE
HDLNGHIRGKRIDVKSLKNLSNGCYFPLSVYAMSLDGKVIEETGLGKYIGEPDRLCLPIL
EECCCCCCCCEECHHHHHHCCCCCEEEHEEEEEECCCEEEECCCCCCCCCCCCCCHHHHH
GSLQPSALSPELNAQLYLSMQEEDGSGCRYEPRNILKKLLNQLHANNYFPVMAAELEFYL
CCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEE
FSPQHQSEICSENQCFDIDAPNNYQQVLDEVEKAALLQSIEITAIVAESSPGQYELNLQH
ECCCHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHEEEEEEEEECCCCEEEEEECC
NHDILKLCDQINALKRIVKQVARKHDLTACFMAKPNLAKAGSGMHFHMSLLNQYHQNIFS
CCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHCCCCCEEHHHHHHHHHHHHHC
SEEEKDELATKLLSAISGLIELMPASMAILAPNINSYRRFKIGHHVPLEANWDTNNRNVA
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEECCCCCCEECCCCCCCCEEE
IRIPCSDAQNQRLEYRVAGADCNPYLVTATILAGASYGLSHKLPLPKPAHLLKFPDEHIL
EEEECCCCCCCCEEEEEECCCCCCEEEEEHHHHCCCCCCCCCCCCCCCHHHHCCCCCEEE
LANNQPEALKIFKGSLILKGYLGADFVEHWYTVKQAEYQSIYSQMTEAEQHWNI
EECCCCCEEEEEECCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]