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The map label for this gene is glpK [H]

Identifier: 213158357

GI number: 213158357

Start: 3193998

End: 3195506

Strand: Reverse

Name: glpK [H]

Synonym: AB57_3087

Alternate gene names: 213158357

Gene position: 3195506-3193998 (Counterclockwise)

Preceding gene: 213157613

Following gene: 213158356

Centisome position: 78.89

GC content: 42.48

Gene sequence:

>1509_bases
ATGTCAAATTGCCCGAAAAAATATATTGTCGCTTTTGATCAGGGAACAACAAGCTCAAGAGCAATTGTTTTAGATCATGA
TGCGAACGTTGTCAGTATTGCTCAAAGAGAATTTACCCAGATTTACCCTCAGCCAGGTTGGGTTGAGCATGATCCTATGG
AAATTTGGGCAACTCAAAGTGCTGTATGGGTCGAAGCATTGGCTCAAGCTGGCATAAAGAGTGAACAAGTTGCGGCAATT
GGAATTACAAACCAACGGGAAACCACTATTGTCTGGGACAAAAAAACAGGAAGACCTATTTACAATGCGATTGTTTGGCA
AAGCCGACAAACAACGGAAATATGCAATCAATTGTATAAAGCAGGTTGGCAAGAATATATCCGTAAGACTACTGGTTTAG
TCATTGATCCATATTTCTCGGCCACAAAAATTAAATGGATTTTGGATCATGTTGAAGGCAGTCGTGAGCGTGCTGAACGG
GGAGAGTTATTATTCGGTACGGTGGACACCTGGCTCATTTGGAAACTAACTAATGGTGCTGTTCATGTCACTGATTTTAC
CAATGCATCTCGAACCATGCTGTTTGATATCGAAAAGCTTGAGTGGGATGAAAAACTTTTACAAGCTTTAGATATTCCTA
GAGCAATGTTGCCAGAGGTTCGTAGTTCATCTGAGGTTTATGGATATACACATACCATTAGTGGACAAGAAGTAGGGATC
CCAATTGCCGGAATCGCGGGTGATCAGCAGGCTGCGCTTTTTGGTCAAATGTGTGTTGAGTCGGGACAGGCTAAAAATAC
CTACGGTACTGGCTGTTTTCTACTCATGAATACGGGTAAAAAGATTGTCCGTTCAGAGCATGGGTTACTCACAACTATTG
CCTGTGGTGCGAGTGGTGAGGTGAATTATGCCTTAGAGGGTGCCGTATTTAACGGTGGTTCTTGCGTACAGTGGTTACGC
GATGAATTAAAGGTTATTAAGAATGCTAAGGATTCCGAGCTTTATGCAACACGTGTAAAGGATAATAATGGTGTATATGT
AGTACCTGCTTTTACCGGATTAGGAGCACCGTATTGGGACCCGACAGCTCGCGGCGCAATTTTTGGTCTGACACGTGGGG
CTAGTATTGAACATATTATCCGCGCAACTCTAGAGTCTATTGCCTTTCAAACTCGGGATGTTTTAGATGCCATGCAACAA
GATGCAGAGGAAGAGCTTCGTACACTCCGTGTTGATGGAGGAGTAACGGAAAATAATTTCTTGATGCAATTTCAGGCCGA
TATTTTAACGACACCGGTAGAGCGTCCAATAATGAAAGAAACTACAGCATTGGGTGCAGCTTTCTTAGCAGGGTTGGCCA
CTGGTTTTTGGCAAGATCTTCATGAGTTAAGAAATAAATCTGCCATAGAAAAGGTTTTTGAACCGAAAATGCCTTCTGAA
CAAGCTGAACTCATTTATAAAGGTTGGCTAAAAGCAGTTAAGCGTAGTCAAAATTGGGCAGAGGATTGA

Upstream 100 bases:

>100_bases
ATAATGATATATTCCAAAGAAAATTTTAAATAAATACAGTTAGGCATGATGTTTTTGCATTGTGGGCTAGATGTGTGTGG
AAAATGAAAGGGACATTCAT

Downstream 100 bases:

>100_bases
ACTTTTTCTTAATTAATTGAATTCAACAGTAGGGTAGTCAATGGATAGTTAAATGCTAGAGGATGCAAAATTGATTATTC
TACTTTTTTGATTGTTGTTT

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MSNCPKKYIVAFDQGTTSSRAIVLDHDANVVSIAQREFTQIYPQPGWVEHDPMEIWATQSAVWVEALAQAGIKSEQVAAI
GITNQRETTIVWDKKTGRPIYNAIVWQSRQTTEICNQLYKAGWQEYIRKTTGLVIDPYFSATKIKWILDHVEGSRERAER
GELLFGTVDTWLIWKLTNGAVHVTDFTNASRTMLFDIEKLEWDEKLLQALDIPRAMLPEVRSSSEVYGYTHTISGQEVGI
PIAGIAGDQQAALFGQMCVESGQAKNTYGTGCFLLMNTGKKIVRSEHGLLTTIACGASGEVNYALEGAVFNGGSCVQWLR
DELKVIKNAKDSELYATRVKDNNGVYVVPAFTGLGAPYWDPTARGAIFGLTRGASIEHIIRATLESIAFQTRDVLDAMQQ
DAEEELRTLRVDGGVTENNFLMQFQADILTTPVERPIMKETTALGAAFLAGLATGFWQDLHELRNKSAIEKVFEPKMPSE
QAELIYKGWLKAVKRSQNWAED

Sequences:

>Translated_502_residues
MSNCPKKYIVAFDQGTTSSRAIVLDHDANVVSIAQREFTQIYPQPGWVEHDPMEIWATQSAVWVEALAQAGIKSEQVAAI
GITNQRETTIVWDKKTGRPIYNAIVWQSRQTTEICNQLYKAGWQEYIRKTTGLVIDPYFSATKIKWILDHVEGSRERAER
GELLFGTVDTWLIWKLTNGAVHVTDFTNASRTMLFDIEKLEWDEKLLQALDIPRAMLPEVRSSSEVYGYTHTISGQEVGI
PIAGIAGDQQAALFGQMCVESGQAKNTYGTGCFLLMNTGKKIVRSEHGLLTTIACGASGEVNYALEGAVFNGGSCVQWLR
DELKVIKNAKDSELYATRVKDNNGVYVVPAFTGLGAPYWDPTARGAIFGLTRGASIEHIIRATLESIAFQTRDVLDAMQQ
DAEEELRTLRVDGGVTENNFLMQFQADILTTPVERPIMKETTALGAAFLAGLATGFWQDLHELRNKSAIEKVFEPKMPSE
QAELIYKGWLKAVKRSQNWAED
>Mature_501_residues
SNCPKKYIVAFDQGTTSSRAIVLDHDANVVSIAQREFTQIYPQPGWVEHDPMEIWATQSAVWVEALAQAGIKSEQVAAIG
ITNQRETTIVWDKKTGRPIYNAIVWQSRQTTEICNQLYKAGWQEYIRKTTGLVIDPYFSATKIKWILDHVEGSRERAERG
ELLFGTVDTWLIWKLTNGAVHVTDFTNASRTMLFDIEKLEWDEKLLQALDIPRAMLPEVRSSSEVYGYTHTISGQEVGIP
IAGIAGDQQAALFGQMCVESGQAKNTYGTGCFLLMNTGKKIVRSEHGLLTTIACGASGEVNYALEGAVFNGGSCVQWLRD
ELKVIKNAKDSELYATRVKDNNGVYVVPAFTGLGAPYWDPTARGAIFGLTRGASIEHIIRATLESIAFQTRDVLDAMQQD
AEEELRTLRVDGGVTENNFLMQFQADILTTPVERPIMKETTALGAAFLAGLATGFWQDLHELRNKSAIEKVFEPKMPSEQ
AELIYKGWLKAVKRSQNWAED

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI42794763, Length=506, Percent_Identity=48.4189723320158, Blast_Score=450, Evalue=1e-126,
Organism=Homo sapiens, GI189409120, Length=502, Percent_Identity=48.605577689243, Blast_Score=450, Evalue=1e-126,
Organism=Homo sapiens, GI4504007, Length=502, Percent_Identity=48.605577689243, Blast_Score=449, Evalue=1e-126,
Organism=Homo sapiens, GI41393575, Length=506, Percent_Identity=47.6284584980237, Blast_Score=442, Evalue=1e-124,
Organism=Homo sapiens, GI88196792, Length=509, Percent_Identity=36.5422396856582, Blast_Score=335, Evalue=8e-92,
Organism=Escherichia coli, GI1790361, Length=496, Percent_Identity=71.7741935483871, Blast_Score=759, Evalue=0.0,
Organism=Escherichia coli, GI1789987, Length=489, Percent_Identity=26.5848670756646, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1790005, Length=503, Percent_Identity=23.8568588469185, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787789, Length=491, Percent_Identity=24.2362525458248, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI1789138, Length=511, Percent_Identity=20.9393346379648, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1789168, Length=495, Percent_Identity=21.8181818181818, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17535599, Length=504, Percent_Identity=43.0555555555556, Blast_Score=396, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI71986140, Length=521, Percent_Identity=25.7197696737044, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI71986147, Length=295, Percent_Identity=31.5254237288136, Blast_Score=133, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6321755, Length=557, Percent_Identity=36.8043087971275, Blast_Score=314, Evalue=2e-86,
Organism=Drosophila melanogaster, GI17864214, Length=506, Percent_Identity=44.4664031620553, Blast_Score=418, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24655555, Length=498, Percent_Identity=43.3734939759036, Blast_Score=400, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24655560, Length=498, Percent_Identity=43.3734939759036, Blast_Score=400, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24655570, Length=498, Percent_Identity=43.3734939759036, Blast_Score=400, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24655574, Length=498, Percent_Identity=43.3734939759036, Blast_Score=400, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24655565, Length=498, Percent_Identity=43.3734939759036, Blast_Score=400, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24654573, Length=443, Percent_Identity=46.0496613995485, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI19922058, Length=531, Percent_Identity=36.5348399246704, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI24652871, Length=493, Percent_Identity=37.1196754563895, Blast_Score=307, Evalue=1e-83,
Organism=Drosophila melanogaster, GI24656367, Length=516, Percent_Identity=30.0387596899225, Blast_Score=236, Evalue=3e-62,
Organism=Drosophila melanogaster, GI21357867, Length=532, Percent_Identity=29.1353383458647, Blast_Score=231, Evalue=7e-61,
Organism=Drosophila melanogaster, GI24656372, Length=363, Percent_Identity=31.9559228650138, Blast_Score=182, Evalue=3e-46,
Organism=Drosophila melanogaster, GI45552909, Length=303, Percent_Identity=29.3729372937294, Blast_Score=123, Evalue=3e-28,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 55928; Mature: 55797

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00933 FGGY_KINASES_1 ; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNCPKKYIVAFDQGTTSSRAIVLDHDANVVSIAQREFTQIYPQPGWVEHDPMEIWATQS
CCCCCCEEEEEEECCCCCCCEEEEECCCCEEHHHHHHHHHHCCCCCCCCCCCHHEEECCH
AVWVEALAQAGIKSEQVAAIGITNQRETTIVWDKKTGRPIYNAIVWQSRQTTEICNQLYK
HHHHHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCHHHHHHHCCCCCHHHHHHHHHH
AGWQEYIRKTTGLVIDPYFSATKIKWILDHVEGSRERAERGELLFGTVDTWLIWKLTNGA
HHHHHHHHHHCCEEEECCCCHHHHHHHHHHHCCCHHHHHCCCEEEEECCEEEEEEEECCE
VHVTDFTNASRTMLFDIEKLEWDEKLLQALDIPRAMLPEVRSSSEVYGYTHTISGQEVGI
EEEEECCCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEEECCCCCCCC
PIAGIAGDQQAALFGQMCVESGQAKNTYGTGCFLLMNTGKKIVRSEHGLLTTIACGASGE
EEEECCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHHHCCCCEEEEEEECCCCC
VNYALEGAVFNGGSCVQWLRDELKVIKNAKDSELYATRVKDNNGVYVVPAFTGLGAPYWD
EEEEEECEEECCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCC
PTARGAIFGLTRGASIEHIIRATLESIAFQTRDVLDAMQQDAEEELRTLRVDGGVTENNF
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCE
LMQFQADILTTPVERPIMKETTALGAAFLAGLATGFWQDLHELRNKSAIEKVFEPKMPSE
EEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
QAELIYKGWLKAVKRSQNWAED
HHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SNCPKKYIVAFDQGTTSSRAIVLDHDANVVSIAQREFTQIYPQPGWVEHDPMEIWATQS
CCCCCEEEEEEECCCCCCCEEEEECCCCEEHHHHHHHHHHCCCCCCCCCCCHHEEECCH
AVWVEALAQAGIKSEQVAAIGITNQRETTIVWDKKTGRPIYNAIVWQSRQTTEICNQLYK
HHHHHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCHHHHHHHCCCCCHHHHHHHHHH
AGWQEYIRKTTGLVIDPYFSATKIKWILDHVEGSRERAERGELLFGTVDTWLIWKLTNGA
HHHHHHHHHHCCEEEECCCCHHHHHHHHHHHCCCHHHHHCCCEEEEECCEEEEEEEECCE
VHVTDFTNASRTMLFDIEKLEWDEKLLQALDIPRAMLPEVRSSSEVYGYTHTISGQEVGI
EEEEECCCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEEECCCCCCCC
PIAGIAGDQQAALFGQMCVESGQAKNTYGTGCFLLMNTGKKIVRSEHGLLTTIACGASGE
EEEECCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHHHCCCCEEEEEEECCCCC
VNYALEGAVFNGGSCVQWLRDELKVIKNAKDSELYATRVKDNNGVYVVPAFTGLGAPYWD
EEEEEECEEECCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCC
PTARGAIFGLTRGASIEHIIRATLESIAFQTRDVLDAMQQDAEEELRTLRVDGGVTENNF
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCE
LMQFQADILTTPVERPIMKETTALGAAFLAGLATGFWQDLHELRNKSAIEKVFEPKMPSE
EEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
QAELIYKGWLKAVKRSQNWAED
HHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA