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The map label for this gene is thiE [H]

Identifier: 213158179

GI number: 213158179

Start: 2987446

End: 2988057

Strand: Reverse

Name: thiE [H]

Synonym: AB57_2896

Alternate gene names: 213158179

Gene position: 2988057-2987446 (Counterclockwise)

Preceding gene: 213158180

Following gene: 213158178

Centisome position: 73.77

GC content: 41.83

Gene sequence:

>612_bases
ATGCGCGGTTTATACCTCATTACCAATGATGACCCAATCCAATTATTATTAGAAAAATTAGACGCCGCACTCGCAACCCG
TCAAATCGCAATTTTACAGTACCGCCGTAAAAAAGTAGACAAAGCCGACCAACCTGCTGAAGTTGAACAGATCAAACAAT
TATGTGAAAAATATCAGGTTCCTTTTGTCATTAATGATGACCTAAAACTGGCTGCTCAGTTTGGTTTAGGTGTGCATTTA
GGTCAAAGCGATGGCGAAATCACCGATGCAAAATCGCAGTTACCAGAAGGTGTTATTATTGGCCGTACTTGCTTAAACTC
ATTAGAACTTGCCCAAAAAGCTATTGCCGATGGTGCAACTTATGTTGCCTTTGGTGCGGTTTATGCAACTGCCACTAAAC
CGGAGGCTGGCAATGTAGGTATAGAAGTGATTAAGCAAGCAGCAGCTCAATATGATTTACCTATTTGTGCAATTGGTGGT
TTGACTGTCGAAAATTCAAAACCTGTGATTGAGGCCGGAGCTGACCTCTGTGCAGTCATTAGTGATATATTAGGTCGATC
AACTGCTGAAATTCCTGCCCGTGTACAGGCGTGGGCAAAATTATTTTCTTAA

Upstream 100 bases:

>100_bases
AAGATAAAAAAACAAATGATCACTGCTTAGTATTCTTTATCTTTAAATTTTAAAGAAGAAAATATATTCTGAGCTCAAGA
TATTTAGTGGTGAAATTTTT

Downstream 100 bases:

>100_bases
GCTTTCAAAATTATTTTTTAGATATTTAAGACGAGCATTTCCATGAGTTTATCTCCAAAGCAAGAACAGTTATTTAAACA
AGCCAGCAAACACATTCCAG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MRGLYLITNDDPIQLLLEKLDAALATRQIAILQYRRKKVDKADQPAEVEQIKQLCEKYQVPFVINDDLKLAAQFGLGVHL
GQSDGEITDAKSQLPEGVIIGRTCLNSLELAQKAIADGATYVAFGAVYATATKPEAGNVGIEVIKQAAAQYDLPICAIGG
LTVENSKPVIEAGADLCAVISDILGRSTAEIPARVQAWAKLFS

Sequences:

>Translated_203_residues
MRGLYLITNDDPIQLLLEKLDAALATRQIAILQYRRKKVDKADQPAEVEQIKQLCEKYQVPFVINDDLKLAAQFGLGVHL
GQSDGEITDAKSQLPEGVIIGRTCLNSLELAQKAIADGATYVAFGAVYATATKPEAGNVGIEVIKQAAAQYDLPICAIGG
LTVENSKPVIEAGADLCAVISDILGRSTAEIPARVQAWAKLFS
>Mature_203_residues
MRGLYLITNDDPIQLLLEKLDAALATRQIAILQYRRKKVDKADQPAEVEQIKQLCEKYQVPFVINDDLKLAAQFGLGVHL
GQSDGEITDAKSQLPEGVIIGRTCLNSLELAQKAIADGATYVAFGAVYATATKPEAGNVGIEVIKQAAAQYDLPICAIGG
LTVENSKPVIEAGADLCAVISDILGRSTAEIPARVQAWAKLFS

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=184, Percent_Identity=31.5217391304348, Blast_Score=71, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6325042, Length=203, Percent_Identity=27.0935960591133, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 21750; Mature: 21750

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGLYLITNDDPIQLLLEKLDAALATRQIAILQYRRKKVDKADQPAEVEQIKQLCEKYQV
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCC
PFVINDDLKLAAQFGLGVHLGQSDGEITDAKSQLPEGVIIGRTCLNSLELAQKAIADGAT
CEEECCCHHHHHHHCCEEEECCCCCCCCCHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCH
YVAFGAVYATATKPEAGNVGIEVIKQAAAQYDLPICAIGGLTVENSKPVIEAGADLCAVI
HHHHHHHHEECCCCCCCCHHHHHHHHHHHHCCCCEEEECCEEECCCCCHHHHCHHHHHHH
SDILGRSTAEIPARVQAWAKLFS
HHHHCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MRGLYLITNDDPIQLLLEKLDAALATRQIAILQYRRKKVDKADQPAEVEQIKQLCEKYQV
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCC
PFVINDDLKLAAQFGLGVHLGQSDGEITDAKSQLPEGVIIGRTCLNSLELAQKAIADGAT
CEEECCCHHHHHHHCCEEEECCCCCCCCCHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCH
YVAFGAVYATATKPEAGNVGIEVIKQAAAQYDLPICAIGGLTVENSKPVIEAGADLCAVI
HHHHHHHHEECCCCCCCCHHHHHHHHHHHHCCCCEEEECCEEECCCCCHHHHCHHHHHHH
SDILGRSTAEIPARVQAWAKLFS
HHHHCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA