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The map label for this gene is opuD [H]

Identifier: 213158131

GI number: 213158131

Start: 2927099

End: 2928775

Strand: Direct

Name: opuD [H]

Synonym: AB57_2843

Alternate gene names: 213158131

Gene position: 2927099-2928775 (Clockwise)

Preceding gene: 213158130

Following gene: 213158132

Centisome position: 72.26

GC content: 38.04

Gene sequence:

>1677_bases
ATGCGTACAACAAGGGTTGCTGAATCCAATTCTGCGGTTTCAATTATTTCAAAAATATTAGTAGTCGCATTTGTACTTTT
TTGCGCAATTTATGCAACCGAAGCAGCGAAAATATTTGCTGATTTATCAAGCGCCTTACTGCGCAATATGAAATGGTTCA
TTTTGCTATCTGTTACAGCGATCTGCTTTTTCTTAATTTATATCATGTGCAGCCGTTATGGTTTAATAAAACTGGGAAAA
GATACAGATAAACCTGAATACAGTTATTTTGCATGGATTTCGATGTTGTTTTCATGTGCCATGGGAGTCGGTTTAGTATT
TTGGGGCGTTGCTGAACCAATGAACCATTATGCGAGTAATCCATTTACAAAAGGTTTCACGGATGAAGCAGCTAGTATGG
CAATGCAAATTACCTACTTTCACTGGGGATTACATGCATGGTCTGTTTACTGTATTGCAGCGTTAGCAATCGGTTATTTT
TCTTATCGTAAAGGTCTACCTTTTTCTTTACGTTCAATTTTACATCCGCTTATTGGCGATCGTATTTATGGTCCTATCGG
ACATTTTATTGATATTGTCGTAATTGTTGTCACAACTTTTGGTATCTCTCAAACTCTCGCTTTAGGTGTTTTACAAATAA
ATGCCGGATTACATAAAGTTTTTGGCCTAGAAACCAACACAACTGTTCAATTTATTCTCATGATTACCTTATCCAGTATT
GCCACTTTCTCTGTAGTGCGCGGTATTGGTACAGGAATGCGCCGTATTTCTGAACTTAATATTATCCTATCAATCATTTT
GATTGTAATCCTTATGCTCATTGGACCTGCTAGATACATTACTAATTCTTACTTAGAAGGTATTGGTAACTATACATCTA
ATTTTGTAGGTATGAGTTTGTGGAGCGATACTCAAAAAGACTCTGAGTGGCAAAACTGGTGGACAGCTTTCTATTGGTTA
TGGTGGTCAACTTGGGCTCCATTTGTAGGTATGTTCATTGCTAAAATTTCAAAAGGACGTACTATCCGTCAACTCATTGC
AGGTGTGCTTGTTGTTCCAACTTTGGTTACTTTTTTCTGGTTTGCAGTGTTCGGCGGTTCAGCTCTAAAAATTGAGCAAG
ACTCTCGCCATGCATTTGAAAAACAGCAATCAGCTTTAGTAGAGAATACGCAATCAACCACTGCAACAACACATCCTACC
ATTACTTCTCAAACTGAAGATAAAGCAGAACCACAGGAAGAGTTTACTGGTGGTCCTATAGTAAAAGCGACACAAAAAGA
CAGTACCCTCGCCGTTTTTGCTTTATTTGATGCTGTTGGAGGAGGAGGTATTTTTGGACAAATTCTTAGTATTGCAACCT
GTCTTCTACTCATTACTTATCTTGTAACATCACAAGATGGCGGAACACATGTTCTATGTTTCCTTGATACCTTATCTGAA
AAAGACACTCCTATCCGTACTCGTCTTTTGTGGTGTGTGTTTGTTACTGCAATCAGCCTAGGTTTGCTTTATGTAGGAGG
TCTCAAAGCGATTCAAGCCGCTGTCACACTATTTGGATTCCCTATTATTGTTTTGCTCACAATTATGGCTGTAGCACTTA
TGAAAGCTTTCAGGCAAGAAGATATAGCAAATATTAACGTTGTACCTAAACATCTGAAGATTGAGCCTGAAGCATAA

Upstream 100 bases:

>100_bases
ATAAATCGGTTGAGCAAAATTAGTACTCCTCACTACTCTATATTATTCGTATAAAACAGCTAATGATTCTAGCTGTTTAC
CACGCATCCAAGGAGGAGTT

Downstream 100 bases:

>100_bases
TCTAGACTTCTATCTCACAGTTTAGAAGAAAGTTATAATCTAAAACTCTCTTCTAAACTTATATTTTCAATTTAAAACAA
ATAGTTAAACCATAAGAAAT

Product: glycine betaine transporter OpuD

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 558; Mature: 558

Protein sequence:

>558_residues
MRTTRVAESNSAVSIISKILVVAFVLFCAIYATEAAKIFADLSSALLRNMKWFILLSVTAICFFLIYIMCSRYGLIKLGK
DTDKPEYSYFAWISMLFSCAMGVGLVFWGVAEPMNHYASNPFTKGFTDEAASMAMQITYFHWGLHAWSVYCIAALAIGYF
SYRKGLPFSLRSILHPLIGDRIYGPIGHFIDIVVIVVTTFGISQTLALGVLQINAGLHKVFGLETNTTVQFILMITLSSI
ATFSVVRGIGTGMRRISELNIILSIILIVILMLIGPARYITNSYLEGIGNYTSNFVGMSLWSDTQKDSEWQNWWTAFYWL
WWSTWAPFVGMFIAKISKGRTIRQLIAGVLVVPTLVTFFWFAVFGGSALKIEQDSRHAFEKQQSALVENTQSTTATTHPT
ITSQTEDKAEPQEEFTGGPIVKATQKDSTLAVFALFDAVGGGGIFGQILSIATCLLLITYLVTSQDGGTHVLCFLDTLSE
KDTPIRTRLLWCVFVTAISLGLLYVGGLKAIQAAVTLFGFPIIVLLTIMAVALMKAFRQEDIANINVVPKHLKIEPEA

Sequences:

>Translated_558_residues
MRTTRVAESNSAVSIISKILVVAFVLFCAIYATEAAKIFADLSSALLRNMKWFILLSVTAICFFLIYIMCSRYGLIKLGK
DTDKPEYSYFAWISMLFSCAMGVGLVFWGVAEPMNHYASNPFTKGFTDEAASMAMQITYFHWGLHAWSVYCIAALAIGYF
SYRKGLPFSLRSILHPLIGDRIYGPIGHFIDIVVIVVTTFGISQTLALGVLQINAGLHKVFGLETNTTVQFILMITLSSI
ATFSVVRGIGTGMRRISELNIILSIILIVILMLIGPARYITNSYLEGIGNYTSNFVGMSLWSDTQKDSEWQNWWTAFYWL
WWSTWAPFVGMFIAKISKGRTIRQLIAGVLVVPTLVTFFWFAVFGGSALKIEQDSRHAFEKQQSALVENTQSTTATTHPT
ITSQTEDKAEPQEEFTGGPIVKATQKDSTLAVFALFDAVGGGGIFGQILSIATCLLLITYLVTSQDGGTHVLCFLDTLSE
KDTPIRTRLLWCVFVTAISLGLLYVGGLKAIQAAVTLFGFPIIVLLTIMAVALMKAFRQEDIANINVVPKHLKIEPEA
>Mature_558_residues
MRTTRVAESNSAVSIISKILVVAFVLFCAIYATEAAKIFADLSSALLRNMKWFILLSVTAICFFLIYIMCSRYGLIKLGK
DTDKPEYSYFAWISMLFSCAMGVGLVFWGVAEPMNHYASNPFTKGFTDEAASMAMQITYFHWGLHAWSVYCIAALAIGYF
SYRKGLPFSLRSILHPLIGDRIYGPIGHFIDIVVIVVTTFGISQTLALGVLQINAGLHKVFGLETNTTVQFILMITLSSI
ATFSVVRGIGTGMRRISELNIILSIILIVILMLIGPARYITNSYLEGIGNYTSNFVGMSLWSDTQKDSEWQNWWTAFYWL
WWSTWAPFVGMFIAKISKGRTIRQLIAGVLVVPTLVTFFWFAVFGGSALKIEQDSRHAFEKQQSALVENTQSTTATTHPT
ITSQTEDKAEPQEEFTGGPIVKATQKDSTLAVFALFDAVGGGGIFGQILSIATCLLLITYLVTSQDGGTHVLCFLDTLSE
KDTPIRTRLLWCVFVTAISLGLLYVGGLKAIQAAVTLFGFPIIVLLTIMAVALMKAFRQEDIANINVVPKHLKIEPEA

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=516, Percent_Identity=35.4651162790698, Blast_Score=320, Evalue=1e-88,
Organism=Escherichia coli, GI1788102, Length=494, Percent_Identity=29.7570850202429, Blast_Score=205, Evalue=7e-54,
Organism=Escherichia coli, GI1786224, Length=531, Percent_Identity=22.0338983050847, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 61699; Mature: 61699

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCEECCCCEECCCC
>Mature Secondary Structure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CCCCCCEECCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]