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The map label for this gene is opuD [H]

Identifier: 213158130

GI number: 213158130

Start: 2925184

End: 2926881

Strand: Direct

Name: opuD [H]

Synonym: AB57_2842

Alternate gene names: 213158130

Gene position: 2925184-2926881 (Clockwise)

Preceding gene: 213158122

Following gene: 213158131

Centisome position: 72.22

GC content: 37.22

Gene sequence:

>1698_bases
ATGCGTACAACAACAGGAGTCATGAAAGGGCTTAATCCGACTGTTACTATTGTCTCGAAGTTATTACTCTTCATTTTTAT
CGCTTTTTGCATTTTTAAGGAAAAACAGGCAGGTGAATATTTTCAGCTGATATCTAATACCTTATTACAAAATGCAAAAT
GGTATCTCTTGTTACTAGCCACAAGCTTATTAGTATTTCTTCTTTACTTATGTATGAGTCGTTTTGGACAAATAAAATTA
GGAAAAGATAACGAAAAGCCTGAATATAGTAATTTGGCCTGGATATGTATGCTTTTCTCATGCGGTATGGGAATTGGCCT
AATGTTTTGGTCTGTAGCTGAGCCAATCAGTCACTATGCTAGCAATCCTTTTACTCAAGGGCTATCTGATGAATCAGCCC
GTATGGCAATGCAGTTAACATTTTTCCATTGGGGACTACACCCATGGGGACTGTTTTGTTTAGTAGCTGTTGCTTTAGGG
TATTTTGCATATCGTAAAGACTTACCTTTCGCAATGCGCTCAGTCTTATATCCTCTGATTGGGAAAAAAATATATGGTCC
AATTGGAGATATAGTCGATACATTAACTATTGTCATTACCGCTTTTGGTATTTCCCAATCATTAAGTATGGGGATTCTTG
AGCTAAATACAGGCCTTAATCATACTTTTGGTATTAGTATGGATGTGAAAATCCAATTAATCCTAGTACTAGTACTTTGT
GCACTAGCAACTCTCTCACTTTTATCTGCAATAAGTAAAGGATTTAAAATTATTGCCGAAGGTAATATGGTTCTTTCCTA
CGTTCTAGTATTTGCTATTATCTTTATTGGTTCATCAAGTTACATTTTAAATACTTTTTTTGAAGGTATTGGTGACTATC
TACAGAATATTATTGGGATGAGTTTATGGAGTGATGCTCAGAAAGATTCTGGCTGGCAAAATTTATGGACCGCTTATTAT
TGGCCGTGGTGGATGACTTGGGCGCCATTTGTAGGGTTATTTATTGCCCGAATATCAAAAGGGCGTACCATTCGCCAAGT
TATCTTAGGCTCACTTGCAGCCCCCACTTTACTTACTTTTCTTTGGATAGCCGCATTTGGTGGGGCCGCACTGAAAATAG
AGAAAACTTCCCACCAACAAACATCTTCAACAATTGTTACGAATATCCCTTCTAGTACATCAGTCGATAAAGTTCCAACT
CACGAACTTACTATCACCGAAGCGACTCAACAAGATCCAGCACGAGCGGTATTCGTGTTCTTTGACCATCTCACTCCAAA
CTCTGATACTAAAATCATTCTCAGCGCATTACTTTGCTTACTATTAGCCATTCATGTGATTACCTTAGTTAACTCTGGAA
TATTAGTACTTTGTTTCTTAGATTCTTCTGGTGCAACGGACCCATCAATCGGAATTCGGCTGATTTGGAGTGCTATTATT
CCTTTAATTGGTGGAGGACTACTACTAGCTGGCGGTCTAACTGCTATAAAAACAGCTTGTATTATTGCGGGTTTTCCTAT
CTCGATTCTATTAGGAATTATGGGTTTCTCATTAATAAAAAGCTTACGACAAGATCCGAAATTTCAGGAAAATGAAATAG
TTTCTATTCAAGCCGATGCTTCCCCCTTGGAGAAAAAATTAGTTCAAACTCCTAAAAAAGAGGATAAAGAATTAAATGAT
CAACTGATTGTAGAGTAA

Upstream 100 bases:

>100_bases
AAATCAGTTGAGCTATCTTCTCCCACCTCACTACCCTGTAATATTAGTATTAAGCAGCTAATCGTTTTTTAGCTGTTGGA
AACGCATCCAAGGAGTAGTT

Downstream 100 bases:

>100_bases
TTAACTATTATCTCCAATTGGCTAGAAGTAAAATCTTCTAGCCAATTTTATAATGTGTAATTTTTCATATTGTCTTCTAT
CCAACTACCTATGATAAAAA

Product: glycine betaine transporter OpuD

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 565; Mature: 565

Protein sequence:

>565_residues
MRTTTGVMKGLNPTVTIVSKLLLFIFIAFCIFKEKQAGEYFQLISNTLLQNAKWYLLLLATSLLVFLLYLCMSRFGQIKL
GKDNEKPEYSNLAWICMLFSCGMGIGLMFWSVAEPISHYASNPFTQGLSDESARMAMQLTFFHWGLHPWGLFCLVAVALG
YFAYRKDLPFAMRSVLYPLIGKKIYGPIGDIVDTLTIVITAFGISQSLSMGILELNTGLNHTFGISMDVKIQLILVLVLC
ALATLSLLSAISKGFKIIAEGNMVLSYVLVFAIIFIGSSSYILNTFFEGIGDYLQNIIGMSLWSDAQKDSGWQNLWTAYY
WPWWMTWAPFVGLFIARISKGRTIRQVILGSLAAPTLLTFLWIAAFGGAALKIEKTSHQQTSSTIVTNIPSSTSVDKVPT
HELTITEATQQDPARAVFVFFDHLTPNSDTKIILSALLCLLLAIHVITLVNSGILVLCFLDSSGATDPSIGIRLIWSAII
PLIGGGLLLAGGLTAIKTACIIAGFPISILLGIMGFSLIKSLRQDPKFQENEIVSIQADASPLEKKLVQTPKKEDKELND
QLIVE

Sequences:

>Translated_565_residues
MRTTTGVMKGLNPTVTIVSKLLLFIFIAFCIFKEKQAGEYFQLISNTLLQNAKWYLLLLATSLLVFLLYLCMSRFGQIKL
GKDNEKPEYSNLAWICMLFSCGMGIGLMFWSVAEPISHYASNPFTQGLSDESARMAMQLTFFHWGLHPWGLFCLVAVALG
YFAYRKDLPFAMRSVLYPLIGKKIYGPIGDIVDTLTIVITAFGISQSLSMGILELNTGLNHTFGISMDVKIQLILVLVLC
ALATLSLLSAISKGFKIIAEGNMVLSYVLVFAIIFIGSSSYILNTFFEGIGDYLQNIIGMSLWSDAQKDSGWQNLWTAYY
WPWWMTWAPFVGLFIARISKGRTIRQVILGSLAAPTLLTFLWIAAFGGAALKIEKTSHQQTSSTIVTNIPSSTSVDKVPT
HELTITEATQQDPARAVFVFFDHLTPNSDTKIILSALLCLLLAIHVITLVNSGILVLCFLDSSGATDPSIGIRLIWSAII
PLIGGGLLLAGGLTAIKTACIIAGFPISILLGIMGFSLIKSLRQDPKFQENEIVSIQADASPLEKKLVQTPKKEDKELND
QLIVE
>Mature_565_residues
MRTTTGVMKGLNPTVTIVSKLLLFIFIAFCIFKEKQAGEYFQLISNTLLQNAKWYLLLLATSLLVFLLYLCMSRFGQIKL
GKDNEKPEYSNLAWICMLFSCGMGIGLMFWSVAEPISHYASNPFTQGLSDESARMAMQLTFFHWGLHPWGLFCLVAVALG
YFAYRKDLPFAMRSVLYPLIGKKIYGPIGDIVDTLTIVITAFGISQSLSMGILELNTGLNHTFGISMDVKIQLILVLVLC
ALATLSLLSAISKGFKIIAEGNMVLSYVLVFAIIFIGSSSYILNTFFEGIGDYLQNIIGMSLWSDAQKDSGWQNLWTAYY
WPWWMTWAPFVGLFIARISKGRTIRQVILGSLAAPTLLTFLWIAAFGGAALKIEKTSHQQTSSTIVTNIPSSTSVDKVPT
HELTITEATQQDPARAVFVFFDHLTPNSDTKIILSALLCLLLAIHVITLVNSGILVLCFLDSSGATDPSIGIRLIWSAII
PLIGGGLLLAGGLTAIKTACIIAGFPISILLGIMGFSLIKSLRQDPKFQENEIVSIQADASPLEKKLVQTPKKEDKELND
QLIVE

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=540, Percent_Identity=35.3703703703704, Blast_Score=322, Evalue=4e-89,
Organism=Escherichia coli, GI1788102, Length=505, Percent_Identity=26.1386138613861, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI1786224, Length=496, Percent_Identity=27.4193548387097, Blast_Score=169, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 62206; Mature: 62206

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CHHHHHHHHCCCHHHHCCCCCCCCC
>Mature Secondary Structure
MRTTTGVMKGLNPTVTIVSKLLLFIFIAFCIFKEKQAGEYFQLISNTLLQNAKWYLLLLA
CCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHH
TSLLVFLLYLCMSRFGQIKLGKDNEKPEYSNLAWICMLFSCGMGIGLMFWSVAEPISHYA
HHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
SNPFTQGLSDESARMAMQLTFFHWGLHPWGLFCLVAVALGYFAYRKDLPFAMRSVLYPLI
CCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
GKKIYGPIGDIVDTLTIVITAFGISQSLSMGILELNTGLNHTFGISMDVKIQLILVLVLC
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEECCCCCHHHHHHHHHHHH
ALATLSLLSAISKGFKIIAEGNMVLSYVLVFAIIFIGSSSYILNTFFEGIGDYLQNIIGM
HHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
SLWSDAQKDSGWQNLWTAYYWPWWMTWAPFVGLFIARISKGRTIRQVILGSLAAPTLLTF
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LWIAAFGGAALKIEKTSHQQTSSTIVTNIPSSTSVDKVPTHELTITEATQQDPARAVFVF
HHHHHCCCCEEEEECCCCCHHCCCEEEECCCCCCCCCCCCCCEEEECCCCCCCCCEEEEE
FDHLTPNSDTKIILSALLCLLLAIHVITLVNSGILVLCFLDSSGATDPSIGIRLIWSAII
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHH
PLIGGGLLLAGGLTAIKTACIIAGFPISILLGIMGFSLIKSLRQDPKFQENEIVSIQADA
HHHCCHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCC
SPLEKKLVQTPKKEDKELNDQLIVE
CHHHHHHHHCCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]