Click here to switch to the map view.

The map label for this gene is glnA [H]

Identifier: 213158071

GI number: 213158071

Start: 2852545

End: 2853960

Strand: Reverse

Name: glnA [H]

Synonym: AB57_2783

Alternate gene names: 213158071

Gene position: 2853960-2852545 (Counterclockwise)

Preceding gene: 213158075

Following gene: 213158070

Centisome position: 70.46

GC content: 43.57

Gene sequence:

>1416_bases
ATGAGCATGGCGAACAAGGTCCTTCAACTCATACAAGAAAGTGGCGCAAAATGGGTCGATTTTCGCTTTACTGATACTAA
GGGTAAAGAACAGCACGTAACTTACCCAGCTGATTCTATTGATGAAGATACTTTTGAAGACGGTAAAATGTTCGATGGTT
CTTCAATCGCTGGTTGGAAAGGTATTGAAGCATCTGACATGATTTTACGTCCGGATGCAGAAACTGGTTTCATCGACCCG
TTCTTTGCTGAGCCAACAGTAGTTGTGACTTGTGACGTTATTGAACCTTCAACTGGTCAAGGTTATGAACGTGACCCACG
TTCGATTGCTCGCCGTGCAGAAGAATACTTAAAATCTACAGGTATCGGTGATACTGCATTCTTTGGTCCAGAGCCAGAAT
TCTTTGTATTTGACGAAGTGAAATGGGACATCGATATGTCTGGCGCTCGTCATACATTAATCGCTGAAGAGGCTGCTTGG
TCTACTGGTAAAGATTACGAGTCTGGTAACTCAGGTCACCGTCCACGTGTTAAAGGTGGTTACTTCCCAGTTCCTCCAGT
TGACTCTGCACAAGATATGCGTGCAGAAATGTGTGCAAAAATTGAAGACATTATGGGTCCAGGTCGTGTAGAAGTACACC
ACCACGAAGTTGCTTCTTGCCAATTAGAAATTGGTGTGAGCTTCAATACGCTTGTACGTAAAGCTGACGAAGTTCAACAG
TTCAAATATGCTGTTTGGAACGTTGCACATCAATATGCAAAAACAGCTACGTTTATGCCTAAACCAATGGTAGGTGATAA
CGGTTCTGGTATGCACGTTCACATGTCTATCTCTAAAGATGGTAAGAACTTGTTTGCTGGTGATGAATATGCAGGCCTTT
CAGAAATGGCGTTGTACTTCATCGGTGGTATCATCAAGCACGCTCGTGCATTGAATGCAATCACAAACCCATCTACAAAC
TCATACAAGCGTTTGGTTCCTCACTTCGAAGCACCAATTATGCTTGCTTACTCAGCGCGTAACCGTTCTGCGTCTATCCG
TATTCCTTACGTTTCTAACCCTAAAGGCAAGCGTATCGAAGCTCGTTTCCCAGACCCAATGATGAACCCGTACTTAGGTT
TCGCTGCGTTATTAATGGCTGGTATCGACGGTATCCAAAACAAGATCCATCCGGGCGAAGCTGCTGACAAGAACTTGTAC
GATCTTCCTCCAGAAGAAGAAGCAAAAATCCCAACAGTTGCTCATAGCCTAGATATGGCTCTTGAAGCACTTCAAGCAGA
TCACGAATTCTTGTTAAAAGGTGGCGTGTTCACTAAAGAAATGCTTGATGCATACATCGAACTTAAAACTGAAGATGTAC
GTCGTCTTAACACGACTACTCACCCAGTTGAATTCGATATGTACTACAGCCTGTAA

Upstream 100 bases:

>100_bases
TGTTTTAGGTAAGTCTTATAAAGTTGGTATGAGAATTGCTTAATAAATACCAGCAACTAACACGCACTCAACAAGTGCAA
CAAAATTTCATTGGAGCAAA

Downstream 100 bases:

>100_bases
TACATACTTCATAAAAAAGCCTGCTTATGCAGGCTTTTTTATTGCTTGGAATGTTGATTGAATTCTCTACGTTTTTTATC
AAAAAATTGGGGTAAACTAG

Product: glutamine synthetase, type I

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MSMANKVLQLIQESGAKWVDFRFTDTKGKEQHVTYPADSIDEDTFEDGKMFDGSSIAGWKGIEASDMILRPDAETGFIDP
FFAEPTVVVTCDVIEPSTGQGYERDPRSIARRAEEYLKSTGIGDTAFFGPEPEFFVFDEVKWDIDMSGARHTLIAEEAAW
STGKDYESGNSGHRPRVKGGYFPVPPVDSAQDMRAEMCAKIEDIMGPGRVEVHHHEVASCQLEIGVSFNTLVRKADEVQQ
FKYAVWNVAHQYAKTATFMPKPMVGDNGSGMHVHMSISKDGKNLFAGDEYAGLSEMALYFIGGIIKHARALNAITNPSTN
SYKRLVPHFEAPIMLAYSARNRSASIRIPYVSNPKGKRIEARFPDPMMNPYLGFAALLMAGIDGIQNKIHPGEAADKNLY
DLPPEEEAKIPTVAHSLDMALEALQADHEFLLKGGVFTKEMLDAYIELKTEDVRRLNTTTHPVEFDMYYSL

Sequences:

>Translated_471_residues
MSMANKVLQLIQESGAKWVDFRFTDTKGKEQHVTYPADSIDEDTFEDGKMFDGSSIAGWKGIEASDMILRPDAETGFIDP
FFAEPTVVVTCDVIEPSTGQGYERDPRSIARRAEEYLKSTGIGDTAFFGPEPEFFVFDEVKWDIDMSGARHTLIAEEAAW
STGKDYESGNSGHRPRVKGGYFPVPPVDSAQDMRAEMCAKIEDIMGPGRVEVHHHEVASCQLEIGVSFNTLVRKADEVQQ
FKYAVWNVAHQYAKTATFMPKPMVGDNGSGMHVHMSISKDGKNLFAGDEYAGLSEMALYFIGGIIKHARALNAITNPSTN
SYKRLVPHFEAPIMLAYSARNRSASIRIPYVSNPKGKRIEARFPDPMMNPYLGFAALLMAGIDGIQNKIHPGEAADKNLY
DLPPEEEAKIPTVAHSLDMALEALQADHEFLLKGGVFTKEMLDAYIELKTEDVRRLNTTTHPVEFDMYYSL
>Mature_470_residues
SMANKVLQLIQESGAKWVDFRFTDTKGKEQHVTYPADSIDEDTFEDGKMFDGSSIAGWKGIEASDMILRPDAETGFIDPF
FAEPTVVVTCDVIEPSTGQGYERDPRSIARRAEEYLKSTGIGDTAFFGPEPEFFVFDEVKWDIDMSGARHTLIAEEAAWS
TGKDYESGNSGHRPRVKGGYFPVPPVDSAQDMRAEMCAKIEDIMGPGRVEVHHHEVASCQLEIGVSFNTLVRKADEVQQF
KYAVWNVAHQYAKTATFMPKPMVGDNGSGMHVHMSISKDGKNLFAGDEYAGLSEMALYFIGGIIKHARALNAITNPSTNS
YKRLVPHFEAPIMLAYSARNRSASIRIPYVSNPKGKRIEARFPDPMMNPYLGFAALLMAGIDGIQNKIHPGEAADKNLYD
LPPEEEAKIPTVAHSLDMALEALQADHEFLLKGGVFTKEMLDAYIELKTEDVRRLNTTTHPVEFDMYYSL

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=469, Percent_Identity=66.0980810234542, Blast_Score=656, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=188, Percent_Identity=28.7234042553192, Blast_Score=79, Evalue=6e-16,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52418; Mature: 52287

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMANKVLQLIQESGAKWVDFRFTDTKGKEQHVTYPADSIDEDTFEDGKMFDGSSIAGWK
CCHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEECCCCCCCCCCCCCCCEECCCCCCCCC
GIEASDMILRPDAETGFIDPFFAEPTVVVTCDVIEPSTGQGYERDPRSIARRAEEYLKST
CCCCCCEEECCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHC
GIGDTAFFGPEPEFFVFDEVKWDIDMSGARHTLIAEEAAWSTGKDYESGNSGHRPRVKGG
CCCCCCCCCCCCCEEEEEEEEEEEECCCCCEEEEEHHHCCCCCCCCCCCCCCCCCCCCCC
YFPVPPVDSAQDMRAEMCAKIEDIMGPGRVEVHHHEVASCQLEIGVSFNTLVRKADEVQQ
CCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEEEECCCHHHHHHHHHHHHH
FKYAVWNVAHQYAKTATFMPKPMVGDNGSGMHVHMSISKDGKNLFAGDEYAGLSEMALYF
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHH
IGGIIKHARALNAITNPSTNSYKRLVPHFEAPIMLAYSARNRSASIRIPYVSNPKGKRIE
HHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCEEE
ARFPDPMMNPYLGFAALLMAGIDGIQNKIHPGEAADKNLYDLPPEEEAKIPTVAHSLDMA
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LEALQADHEFLLKGGVFTKEMLDAYIELKTEDVRRLNTTTHPVEFDMYYSL
HHHHHCCCHHHHCCCCHHHHHHHHHHHHCHHHHHHHCCCCCCEEEEEEECC
>Mature Secondary Structure 
SMANKVLQLIQESGAKWVDFRFTDTKGKEQHVTYPADSIDEDTFEDGKMFDGSSIAGWK
CHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEECCCCCCCCCCCCCCCEECCCCCCCCC
GIEASDMILRPDAETGFIDPFFAEPTVVVTCDVIEPSTGQGYERDPRSIARRAEEYLKST
CCCCCCEEECCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHC
GIGDTAFFGPEPEFFVFDEVKWDIDMSGARHTLIAEEAAWSTGKDYESGNSGHRPRVKGG
CCCCCCCCCCCCCEEEEEEEEEEEECCCCCEEEEEHHHCCCCCCCCCCCCCCCCCCCCCC
YFPVPPVDSAQDMRAEMCAKIEDIMGPGRVEVHHHEVASCQLEIGVSFNTLVRKADEVQQ
CCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEEEECCCHHHHHHHHHHHHH
FKYAVWNVAHQYAKTATFMPKPMVGDNGSGMHVHMSISKDGKNLFAGDEYAGLSEMALYF
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHH
IGGIIKHARALNAITNPSTNSYKRLVPHFEAPIMLAYSARNRSASIRIPYVSNPKGKRIE
HHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCEEE
ARFPDPMMNPYLGFAALLMAGIDGIQNKIHPGEAADKNLYDLPPEEEAKIPTVAHSLDMA
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LEALQADHEFLLKGGVFTKEMLDAYIELKTEDVRRLNTTTHPVEFDMYYSL
HHHHHCCCHHHHCCCCHHHHHHHHHHHHCHHHHHHHCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1969923 [H]