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The map label for this gene is metF [H]

Identifier: 213158056

GI number: 213158056

Start: 2835548

End: 2836384

Strand: Direct

Name: metF [H]

Synonym: AB57_2768

Alternate gene names: 213158056

Gene position: 2835548-2836384 (Clockwise)

Preceding gene: 213158055

Following gene: 213158057

Centisome position: 70.0

GC content: 40.74

Gene sequence:

>837_bases
ATGACTAAACGTACACCTATTTCTTTTGAGTTTTTTCCGCCAAAAACTGATGCCGGTGCTGAAAAACTTCGTATTGTTCA
CCAAGAATTACAGCAATTAAATCCAGAGTTTTTCTCAATTACTTATGGTGCTGGTGGTTCTACTCGTGAACGCACACTTG
CTGCTATTGAAGATTTCAATGGTAAAGGCACTCCTGTTGCCCCTCACCTTTCTTGTATTGGTGATGATAAGACGCGTATT
GCTGAACTACTCGATTTATACAAAGCTCAAGGCATTGACCGTATTGTCGCTTTACGTGGCGACTTACCATCTGGACAGGT
TGGTCTTGGCGAACTTCCTTATGCTCAAGATTTAGTACGTTTTATTCGTGAGCACTCAGGTGATCATTTTCATATTGAAG
TTGCGGCTTATCCAGAAATGCATCCTCAAGCCGAAAGCCTTGACTCAGATATCCAACGTTTTATTGAAAAAGTACAAGCT
GGTGCCAATGCGGGAATTACTCAATTCTTCTTTAATCCAGACTCATACTTTTATTTTATAGAGCGTTTGGATAAAGCTGG
AATTAACATTCCAGTTGCTCCTGGTATCATGCCTATTACCAATGCAAGTAATTTAATTCGCTTTGCAGATGGTACTGGTG
CTGAAATTCCTCGCTGGATTCGTAAGCAATTACAAGCCTATGGCGATGACAGTGAAAGTATTAAAGCTTTTGGTCATGAA
GTTGTTGTTAAGCTATGCGAACGCCTCATTGCAGGTGGTGCACCAAGCTTACACTTCTACTCAATGAACCAAGTCGAACC
AACTCGTCAGCTTGTTGTTGACCTTGGTTTAAATTAA

Upstream 100 bases:

>100_bases
CATTCAAATCTGACGCTTACAAATACTAAGAACTTAAGGGCAGACGGTGAACCGACTGCCCTTTTTTCTAAAAATGTTTC
GCTGTTTACAAGGATTTGAC

Downstream 100 bases:

>100_bases
TTTTGTAAGGCGTACCCAATGAATCGTTTTTTTATCGAAACTGAACTTACAGTTGGCTCAACAATTCAACTAACTGAATC
AGTCTTCCATCATTGGGTAC

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MTKRTPISFEFFPPKTDAGAEKLRIVHQELQQLNPEFFSITYGAGGSTRERTLAAIEDFNGKGTPVAPHLSCIGDDKTRI
AELLDLYKAQGIDRIVALRGDLPSGQVGLGELPYAQDLVRFIREHSGDHFHIEVAAYPEMHPQAESLDSDIQRFIEKVQA
GANAGITQFFFNPDSYFYFIERLDKAGINIPVAPGIMPITNASNLIRFADGTGAEIPRWIRKQLQAYGDDSESIKAFGHE
VVVKLCERLIAGGAPSLHFYSMNQVEPTRQLVVDLGLN

Sequences:

>Translated_278_residues
MTKRTPISFEFFPPKTDAGAEKLRIVHQELQQLNPEFFSITYGAGGSTRERTLAAIEDFNGKGTPVAPHLSCIGDDKTRI
AELLDLYKAQGIDRIVALRGDLPSGQVGLGELPYAQDLVRFIREHSGDHFHIEVAAYPEMHPQAESLDSDIQRFIEKVQA
GANAGITQFFFNPDSYFYFIERLDKAGINIPVAPGIMPITNASNLIRFADGTGAEIPRWIRKQLQAYGDDSESIKAFGHE
VVVKLCERLIAGGAPSLHFYSMNQVEPTRQLVVDLGLN
>Mature_277_residues
TKRTPISFEFFPPKTDAGAEKLRIVHQELQQLNPEFFSITYGAGGSTRERTLAAIEDFNGKGTPVAPHLSCIGDDKTRIA
ELLDLYKAQGIDRIVALRGDLPSGQVGLGELPYAQDLVRFIREHSGDHFHIEVAAYPEMHPQAESLDSDIQRFIEKVQAG
ANAGITQFFFNPDSYFYFIERLDKAGINIPVAPGIMPITNASNLIRFADGTGAEIPRWIRKQLQAYGDDSESIKAFGHEV
VVKLCERLIAGGAPSLHFYSMNQVEPTRQLVVDLGLN

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=279, Percent_Identity=35.4838709677419, Blast_Score=162, Evalue=3e-40,
Organism=Escherichia coli, GI1790377, Length=272, Percent_Identity=39.7058823529412, Blast_Score=198, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI25147436, Length=285, Percent_Identity=33.3333333333333, Blast_Score=158, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI25147441, Length=285, Percent_Identity=33.3333333333333, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6325234, Length=288, Percent_Identity=35.7638888888889, Blast_Score=149, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6321313, Length=266, Percent_Identity=32.3308270676692, Blast_Score=142, Evalue=6e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 30802; Mature: 30671

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRTPISFEFFPPKTDAGAEKLRIVHQELQQLNPEFFSITYGAGGSTRERTLAAIEDFN
CCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCC
GKGTPVAPHLSCIGDDKTRIAELLDLYKAQGIDRIVALRGDLPSGQVGLGELPYAQDLVR
CCCCCCCCCHHCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHHHH
FIREHSGDHFHIEVAAYPEMHPQAESLDSDIQRFIEKVQAGANAGITQFFFNPDSYFYFI
HHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHH
ERLDKAGINIPVAPGIMPITNASNLIRFADGTGAEIPRWIRKQLQAYGDDSESIKAFGHE
HHHHHCCCCCCCCCCCEEECCCCCEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHH
VVVKLCERLIAGGAPSLHFYSMNQVEPTRQLVVDLGLN
HHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCCC
>Mature Secondary Structure 
TKRTPISFEFFPPKTDAGAEKLRIVHQELQQLNPEFFSITYGAGGSTRERTLAAIEDFN
CCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCC
GKGTPVAPHLSCIGDDKTRIAELLDLYKAQGIDRIVALRGDLPSGQVGLGELPYAQDLVR
CCCCCCCCCHHCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHHHH
FIREHSGDHFHIEVAAYPEMHPQAESLDSDIQRFIEKVQAGANAGITQFFFNPDSYFYFI
HHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHH
ERLDKAGINIPVAPGIMPITNASNLIRFADGTGAEIPRWIRKQLQAYGDDSESIKAFGHE
HHHHHCCCCCCCCCCCEEECCCCCEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHH
VVVKLCERLIAGGAPSLHFYSMNQVEPTRQLVVDLGLN
HHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]